LOCUS       NM_015557               9850 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens chromodomain helicase DNA binding protein 5 (CHD5),
            mRNA.
ACCESSION   NM_015557
VERSION     NM_015557.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Otani,R., Sadato,D., Yamada,R., Yajima,H., Kawamura,S., Shimizu,S.,
            Tanaka,S., Takayanagi,S., Takami,H. and Yamaguchi,T.
  TITLE     CHD5 gene variant predicts leptomeningeal metastasis after surgical
            resection of brain metastases of breast cancer
  JOURNAL   J Neurooncol 163 (3), 657-662 (2023)
   PUBMED   37440096
  REMARK    GeneRIF: CHD5 gene variant predicts leptomeningeal metastasis after
            surgical resection of brain metastases of breast cancer.
REFERENCE   2  (bases 1 to 9850)
  AUTHORS   Naeimi,N., Mohseni Kouchesfehani,H., Heidari,Z.,
            Mahmoudzadeh-Sagheb,H. and Movahed,S.
  TITLE     CHD5 gene (rs9434741) might be a genetic risk factor for
            infertility in non-obstructive azoospermia and severe
            oligozoospermia
  JOURNAL   Andrologia 54 (11), e14590 (2022)
   PUBMED   36102082
  REMARK    GeneRIF: CHD5 gene (rs9434741) might be a genetic risk factor for
            infertility in non-obstructive azoospermia and severe
            oligozoospermia.
REFERENCE   3  (bases 1 to 9850)
  AUTHORS   Xu,L., Shao,F., Luo,T., Li,Q., Tan,D. and Tan,Y.
  TITLE     Pan-Cancer Analysis Identifies CHD5 as a Potential Biomarker for
            Glioma
  JOURNAL   Int J Mol Sci 23 (15), 8489 (2022)
   PUBMED   35955624
  REMARK    GeneRIF: Pan-Cancer Analysis Identifies CHD5 as a Potential
            Biomarker for Glioma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9850)
  AUTHORS   Heidari,Z., Asemi-Rad,A., Moudi,B. and Mahmoudzadeh-Sagheb,H.
  TITLE     mRNA expression and epigenetic-based role of chromodomain helicase
            DNA-binding 5 in hepatocellular carcinoma
  JOURNAL   J Int Med Res 50 (7), 3000605221105344 (2022)
   PUBMED   35808817
  REMARK    GeneRIF: mRNA expression and epigenetic-based role of chromodomain
            helicase DNA-binding 5 in hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 9850)
  AUTHORS   Gorringe,K.L., Choong,D.Y., Williams,L.H., Ramakrishna,M.,
            Sridhar,A., Qiu,W., Bearfoot,J.L. and Campbell,I.G.
  TITLE     Mutation and methylation analysis of the chromodomain-helicase-DNA
            binding 5 gene in ovarian cancer
  JOURNAL   Neoplasia 10 (11), 1253-1258 (2008)
   PUBMED   18953434
  REMARK    GeneRIF: study demonstrated the existence of somatic mutations and
            methylation of CHD5 in primary epithelial ovarian cancers; CHD5 may
            play a role as a tumor suppressor gene in ovarian cancer
REFERENCE   6  (bases 1 to 9850)
  AUTHORS   Fujita,T., Igarashi,J., Okawa,E.R., Gotoh,T., Manne,J., Kolla,V.,
            Kim,J., Zhao,H., Pawel,B.R., London,W.B., Maris,J.M., White,P.S.
            and Brodeur,G.M.
  TITLE     CHD5, a tumor suppressor gene deleted from 1p36.31 in
            neuroblastomas
  JOURNAL   J Natl Cancer Inst 100 (13), 940-949 (2008)
   PUBMED   18577749
  REMARK    GeneRIF: CHD5 is the strongest candidate tumor suppressor gene that
            is deleted from 1p36.31 in neuroblastomas, and inactivation of the
            second allele may occur by an epigenetic mechanism.
REFERENCE   7  (bases 1 to 9850)
  AUTHORS   Mulero-Navarro,S. and Esteller,M.
  TITLE     Chromatin remodeling factor CHD5 is silenced by promoter CpG island
            hypermethylation in human cancer
  JOURNAL   Epigenetics 3 (4), 210-215 (2008)
   PUBMED   18698156
  REMARK    GeneRIF: the DNA methylation profiles of CHD family members
            (CHD1-9) in different tumor types.
REFERENCE   8  (bases 1 to 9850)
  AUTHORS   Bagchi,A. and Mills,A.A.
  TITLE     The quest for the 1p36 tumor suppressor
  JOURNAL   Cancer Res 68 (8), 2551-2556 (2008)
   PUBMED   18413720
  REMARK    GeneRIF: A recent functional study identified chromodomain helicase
            DNA-binding protein 5 (CHD5) as a novel tumor suppressor mapping to
            1p36. [REVIEW]
            Review article
REFERENCE   9  (bases 1 to 9850)
  AUTHORS   Bagchi,A., Papazoglu,C., Wu,Y., Capurso,D., Brodt,M., Francis,D.,
            Bredel,M., Vogel,H. and Mills,A.A.
  TITLE     CHD5 is a tumor suppressor at human 1p36
  JOURNAL   Cell 128 (3), 459-475 (2007)
   PUBMED   17289567
  REMARK    GeneRIF: Study demonstrates that Chd5 functions as a tumor
            suppressor in vivo and implicates deletion of CHD5 in human cancer.
REFERENCE   10 (bases 1 to 9850)
  AUTHORS   Thompson,P.M., Gotoh,T., Kok,M., White,P.S. and Brodeur,G.M.
  TITLE     CHD5, a new member of the chromodomain gene family, is
            preferentially expressed in the nervous system
  JOURNAL   Oncogene 22 (7), 1002-1011 (2003)
   PUBMED   12592387
  REMARK    GeneRIF: CDH5 may play a role in the development of the nervous
            system, and it may also play a role in the pathogenesis of neural
            tumors.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL031847.17, AW005809.1, AF425231.1 and AL035406.25.
            
            On Nov 22, 2018 this sequence version replaced NM_015557.2.
            
            Summary: This gene encodes a member of the chromodomain helicase
            DNA-binding protein family. Members of this family are
            characterized by a chromodomain, a helicase ATP-binding domain and
            an additional functional domain. This gene encodes a
            neuron-specific protein that may function in chromatin remodeling
            and gene transcription. This gene is a potential tumor suppressor
            gene that may play a role in the development of neuroblastoma.
            [provided by RefSeq, Feb 2012].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF425231.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262450.8/ ENSP00000262450.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-187               AL031847.17        129542-129728
            188-198             AW005809.1         1-11
            199-6784            AF425231.1         1-6586
            6785-9850           AL035406.25        38593-41658
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.31"
     gene            1..9850
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /note="chromodomain helicase DNA binding protein 5"
                     /db_xref="GeneID:26038"
                     /db_xref="HGNC:HGNC:16816"
                     /db_xref="MIM:610771"
     exon            1..377
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..6163
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /EC_number="3.6.4.12"
                     /note="ATP-dependent helicase CHD5"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 5"
                     /protein_id="NP_056372.1"
                     /db_xref="CCDS:CCDS57.1"
                     /db_xref="GeneID:26038"
                     /db_xref="HGNC:HGNC:16816"
                     /db_xref="MIM:610771"
                     /translation="MRGPVGTEEELPRLFAEEMENEDEMSEEEDGGLEAFDDFFPVEP
                     VSLPKKKKPKKLKENKCKGKRKKKEGSNDELSENEEDLEEKSESEGSDYSPNKKKKKK
                     LKDKKEKKAKRKKKDEDEDDNDDGCLKEPKSSGQLMAEWGLDDVDYLFSEEDYHTLTN
                     YKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGSSAAAAAAAVAAAVE
                     TVTISPPLAVSPPQVPQPVPIRKAKTKEGKGPGVRKKIKGSKDGKKKGKGKKTAGLKF
                     RFGGISNKRKKGSSSEEDEREESDFDSASIHSASVRSECSAALGKKSKRRRKKKRIDD
                     GDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGI
                     QWEPKDDDDEEEEGGCEEEEDDHMEFCRVCKDGGELLCCDACPSSYHLHCLNPPLPEI
                     PNGEWLCPRCTCPPLKGKVQRILHWRWTEPPAPFMVGLPGPDVEPSLPPPKPLEGIPE
                     REFFVKWAGLSYWHCSWVKELQLELYHTVMYRNYQRKNDMDEPPPFDYGSGDEDGKSE
                     KRKNKDPLYAKMEERFYRYGIKPEWMMIHRILNHSFDKKGDVHYLIKWKDLPYDQCTW
                     EIDDIDIPYYDNLKQAYWGHRELMLGEDTRLPKRLLKKGKKLRDDKQEKPPDTPIVDP
                     TVKFDKQPWYIDSTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLY
                     SLYKEGHSKGPYLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRSVIRENEFSFE
                     DNAIRSGKKVFRMKKEVQIKFHVLLTSYELITIDQAILGSIEWACLVVDEAHRLKNNQ
                     SKFFRVLNSYKIDYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKE
                     DQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSQMQKKYYKFILTRNFEALN
                     SKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGSSLVKSSGKLMLLQ
                     KMLKKLRDEGHRVLIFSQMTKMLDLLEDFLEYEGYKYERIDGGITGGLRQEAIDRFNA
                     PGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIY
                     RFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKSGSMTKQELDDILKFGTEELFKDD
                     VEGMMSQGQRPVTPIPDVQSSKGGNLAASAKKKHGSTPPGDNKDVEDSSVIHYDDAAI
                     SKLLDRNQDATDDTELQNMNEYLSSFKVAQYVVREEDGVEEVEREIIKQEENVDPDYW
                     EKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDELSDNQSEYSIGSED
                     EDEDFEERPEGQSGRRQSRRQLKSDRDKPLPPLLARVGGNIEVLGFNARQRKAFLNAI
                     MRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREG
                     LSRQHVLTRIGVMSLVRKKVQEFEHVNGKYSTPDLIPEGPEGKKSGEVISSDPNTPVP
                     ASPAHLLPAPLGLPDKMEAQLGYMDEKDPGAQKPRQPLEVQALPAALDRVESEDKHES
                     PASKERAREERPEETEKAPPSPEQLPREEVLPEKEKILDKLELSLIHSRGDSSELRPD
                     DTKAEEKEPIETQQNGDKEEDDEGKKEDKKGKFKFMFNIADGGFTELHTLWQNEERAA
                     VSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYMILNEPFKSEVHKGNYLEM
                     KNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPNHPAMALNARLAEVECLAESHQHL
                     SKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRIPPVAARLQMSERSIL
                     SRLTNRAGDPTIQQGAFGSSQMYSNNFGPNFRGPGPGGIVNYNQMPLGPYVTDI"
     exon            378..505
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            506..685
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            686..804
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            805..1043
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1168
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1292
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1459
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1681
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1888
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2100
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2232
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2341
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2533
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2734
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..2872
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..2994
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3168
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..3310
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            3311..3442
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            3443..3560
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..3685
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            3686..3917
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            3918..4028
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4201
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4202..4376
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4377..4469
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4470..4558
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4559..4692
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4693..4837
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4838..4997
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            4998..5077
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5078..5210
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5211..5300
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5301..5438
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5439..5547
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5548..5680
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5681..5876
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            5877..6040
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            6041..6155
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            6156..6209
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            6210..9850
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9825..9830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /note="hexamer: AATAAA"
     polyA_site      9850
                     /gene="CHD5"
                     /gene_synonym="CHD-5; PMNDS"
                     /note="major polyA site"
ORIGIN      
        1 gtgccgccgt gcctctggcg ctgccgccgc caccctcggc cggctgcggg actcgggctg
       61 cggggcgcgg tgggaggagc gcccgggctt tgcggggagc aggctaaggc ggccgagaga
      121 aaggggggtc gagacggggg ggtggaggtt tgggggggtg ggggggcagg cggccgccat
      181 cttctcgccg aggccgcctc gcccgccgcg cgcgccggtc cggcagcgca cgggttaagg
      241 ctggcaccgc gcccggcggg agggggggcg cccacctccc ctcctccccg cgccgggcat
      301 gcggggccca gtgggcaccg aggaggagct gccgcggctg ttcgccgagg agatggagaa
      361 tgaggacgag atgtcagaag aagaagatgg tggtcttgaa gccttcgatg actttttccc
      421 tgtggagccc gtgagccttc ctaagaagaa gaaacccaag aagctcaagg aaaacaagtg
      481 taaagggaag cggaagaaga aagaggggag caatgatgag ctatcagaga atgaagagga
      541 tctggaagag aagtcggaga gtgaaggcag tgactactcc ccgaataaaa agaagaagaa
      601 gaaactcaag gacaagaagg agaaaaaagc caagcgaaaa aagaaggatg aggatgagga
      661 tgataatgat gatggatgct taaaggagcc caagtcctcg gggcagctca tggccgagtg
      721 gggcctggac gacgtggact acctgttctc ggaggaggat taccacacgc tgaccaacta
      781 caaggccttc agccagttcc tcaggccact cattgccaag aagaacccga agatccccat
      841 gtccaaaatg atgaccgtcc tgggtgccaa gtggcgggag ttcagcgcca acaacccctt
      901 caagggcagc tccgcggcag cagcggcggc ggcggtggct gcggctgtag agacggtcac
      961 catctcccct ccgctagccg tcagcccccc gcaggtgccc cagcctgtgc ctatccgcaa
     1021 ggccaagacc aaggagggca aagggcctgg agtgaggaag aagatcaaag gctccaaaga
     1081 tgggaagaaa aagggcaaag ggaaaaagac ggccgggctc aagttccgct tcggggggat
     1141 cagcaacaag aggaagaaag gctcctcgag tgaagaagat gagagggagg agtcggactt
     1201 cgacagcgcc agcatccaca gtgcctccgt gcgctccgaa tgctctgcag ccctgggcaa
     1261 gaagagcaag aggaggcgca agaagaagag gattgatgat ggtgacggct atgagacaga
     1321 ccaccaggat tactgtgagg tgtgccagca gggtggggag atcatcctgt gcgacacctg
     1381 cccgagggcc taccatctcg tatgcctgga cccagagctg gagaaggctc ccgagggcaa
     1441 gtggagctgc ccccactgtg agaaggaggg gatccagtgg gagccgaagg acgacgacga
     1501 tgaagaggag gagggcggct gcgaggagga ggaggacgac cacatggagt tctgccgcgt
     1561 gtgcaaggac gggggcgagc tgctctgctg cgacgcctgc ccctcctcct accacctgca
     1621 ttgcctcaac ccgccgctgc ccgagatccc aaacggtgaa tggctctgcc cgcgctgtac
     1681 ttgcccccca ctgaagggca aagtccagcg gattctacac tggaggtgga cggagccccc
     1741 tgcccccttc atggtggggc tgccggggcc tgacgtggag cccagcctcc ctccacctaa
     1801 gcccctggag ggcatccctg agagagagtt ctttgtcaag tgggcagggc tgtcctactg
     1861 gcattgctcc tgggtgaagg agctacagct ggagctgtac cacacggtga tgtatcgcaa
     1921 ctaccaaaga aagaacgaca tggatgagcc gccccccttt gactacggct ctggggatga
     1981 agacggcaag agcgagaaga ggaagaacaa ggaccccctc tatgccaaga tggaggagcg
     2041 cttctaccgc tatggcatca agccagagtg gatgatgatt caccgaatcc tgaaccatag
     2101 ctttgacaag aagggggatg tgcactacct gatcaagtgg aaagacctgc cctacgacca
     2161 gtgcacctgg gagatcgatg acatcgacat cccctactac gacaacctca agcaggccta
     2221 ctggggccac agggagctga tgctgggaga agacaccagg ctgcccaaga ggctgctcaa
     2281 gaagggcaag aagctgaggg acgacaagca ggagaagccg ccggacacgc ccattgtgga
     2341 ccccacggtc aagttcgaca agcagccatg gtacatcgac tccacaggcg gcacactgca
     2401 cccgtaccag ctggagggcc tcaactggct gcgcttctct tgggcccagg gcactgacac
     2461 catcctggcc gatgagatgg gtctgggcaa gacggtgcag accatcgtgt tcctttactc
     2521 cctctacaag gagggccact ccaaagggcc ctacctggtt agcgcgcccc tctccaccat
     2581 catcaactgg gaacgcgagt ttgagatgtg ggcgcccgac ttctacgtgg tcacctacac
     2641 gggggacaag gagagccgct cggtgattcg ggagaacgag ttttcctttg aggacaacgc
     2701 cattcggagt gggaagaagg tattccgtat gaagaaagaa gtgcagatca aattccacgt
     2761 gctgctcacc tcctatgagc tcatcaccat tgaccaggcc atcctgggct ccatcgagtg
     2821 ggcctgcctg gtggtagatg aggcccaccg cctcaagaac aaccagtcca agttttttag
     2881 ggtcttaaac agctacaaga ttgattacaa gctgctgctg acagggaccc cccttcagaa
     2941 caacctggag gagctgttcc atctcctcaa cttcctgact ccagagaggt tcaacaacct
     3001 ggagggcttc ctggaggagt ttgctgacat ctccaaggaa gaccagatca agaagctgca
     3061 tgacctgctg gggccgcaca tgctcaggcg gctcaaggct gacgtgttca agaacatgcc
     3121 ggccaagacc gagctcattg tccgggtgga gctgagccag atgcagaaga agtactacaa
     3181 gttcatcctc acacggaact ttgaggcact gaactccaag gggggcggga accaagtatc
     3241 gctgctcaac atcatgatgg acctgaaaaa gtgctgcaac cacccctacc tcttccctgt
     3301 ggctgccgtg gaggcccctg tcttgcccaa tggctcctac gatggaagct ccctggtcaa
     3361 gtcttcaggg aagctcatgc tgctacagaa gatgctgaag aaactgcggg atgaggggca
     3421 ccgtgtgctc atcttctccc agatgaccaa gatgctggac ctcctggagg acttcctgga
     3481 gtacgaaggc tacaagtatg agcggattga tggtggcatc accgggggcc tccggcagga
     3541 ggcaatcgac agattcaatg cccccggggc ccagcagttc tgcttcctcc tctcaacccg
     3601 ggcaggtggt ctgggcatca acctggccac ggcggacact gtcatcatct acgactcgga
     3661 ctggaacccg cacaatgaca tccaggcctt cagccgcgcc caccgcatcg gccagaacaa
     3721 gaaggtgatg atctaccgct tcgtgactcg ggcctcggtg gaggagcgca tcacgcaggt
     3781 ggccaagcgc aagatgatgc tcacccacct ggtggtgcgg cccggcctcg gctccaagtc
     3841 ggggtccatg accaagcagg agctggacga catcctcaag ttcggcacgg aggaactctt
     3901 caaggacgac gtggagggca tgatgtctca gggccagagg ccggtcacac ccatccctga
     3961 tgtccagtcc tccaaagggg ggaacttggc cgccagtgca aagaagaagc acggtagcac
     4021 cccgccaggt gacaacaagg acgtggagga cagcagtgtg atccactatg acgatgcggc
     4081 catctccaag ctgctggacc ggaaccagga cgctacagat gacacggagc tacagaacat
     4141 gaacgagtac ctgagctcct tcaaggtggc gcagtacgtg gtgcgcgagg aggacggcgt
     4201 ggaggaggtg gagcgggaaa tcatcaagca ggaggagaac gtggaccccg actactggga
     4261 gaagctgctg cggcaccact atgagcagca gcaggaggac ctggcccgca acctgggcaa
     4321 gggcaagcgc atccgcaagc aggtcaacta caacgatgcc tcccaggagg accaggagtg
     4381 gcaggatgag ctctctgata accagtcaga atattccatt ggctctgagg atgaggatga
     4441 ggactttgaa gagaggccgg aagggcagag tggacgacga caatcccgga ggcagctgaa
     4501 gagtgacagg gacaagcccc tgcccccgct tctcgcccga gttggtggca acatcgaggt
     4561 gctgggcttc aatgcccgac agcggaaggc ctttctgaac gccatcatgc gctggggcat
     4621 gcccccgcag gacgccttca actcccactg gctggtgcgg gaccttcgag ggaagagcga
     4681 gaaggagttt agagcctatg tgtccctctt catgcggcac ctgtgtgagc cgggggcgga
     4741 tggtgcagag accttcgcag acggcgtgcc ccgggagggc ctctccaggc agcacgtgct
     4801 gacccgcatc ggggtcatgt cactagttag gaagaaggtt caggagtttg agcatgtcaa
     4861 cgggaagtac agcaccccag acttgatccc tgaggggccc gaggggaaga agtcgggcga
     4921 ggtgatctcc tcggacccca acacaccagt gcccgccagc cctgcccacc tcctgccagc
     4981 cccgctgggc ctgccagaca aaatggaagc ccagctgggc tacatggatg agaaagaccc
     5041 cggggcacag aagccaaggc agcccctgga agtccaggcc cttccagccg ccttggatag
     5101 agtggagagt gaggacaagc acgagagccc agccagcaag gagagagccc gagaggagcg
     5161 gccagaggag acggagaagg ccccgccctc cccggagcag ctgccgagag aggaggtgct
     5221 tcctgagaag gagaagatcc tggacaagct ggagctgagc ttgatccaca gcagagggga
     5281 cagttccgaa ctcaggccag atgacaccaa ggctgaggag aaggagccca ttgaaacaca
     5341 gcaaaatggt gacaaagagg aagatgacga ggggaagaag gaggacaaga aggggaaatt
     5401 caagttcatg ttcaacatcg cggacggggg cttcacggag ttgcacacgc tgtggcagaa
     5461 cgaggagcgg gctgctgtat cctctgggaa aatctacgac atctggcacc ggcgccatga
     5521 ctactggctg ctggcgggca tcgtgacgca cggctacgcc cgctggcagg acatccagaa
     5581 tgacccacgg tacatgatcc tcaacgagcc cttcaagtct gaggtccaca agggcaacta
     5641 cctggagatg aagaacaagt tcctggcccg caggtttaag ctgctggagc aggcgttggt
     5701 cattgaggag cagctccgga gggccgcgta cctgaacatg acgcaggacc ccaaccaccc
     5761 cgccatggcc ctcaacgccc gcctggctga agtggagtgc ctcgccgaga gccaccagca
     5821 cctgtccaag gagtcccttg ctgggaacaa gcctgccaat gccgtcctgc acaaggtcct
     5881 gaaccagctg gaggagctgc tgagcgacat gaaggccgac gtgacccggc tgccatccat
     5941 gctgtcccgc atccccccgg tggccgcccg gctgcagatg tcggagcgca gcatcctgag
     6001 ccgcctgacc aaccgcgccg gggaccccac catccagcag ggcgctttcg gctcctccca
     6061 gatgtacagc aacaactttg ggcccaactt ccggggccct ggaccgggag ggattgtcaa
     6121 ctacaaccag atgcccctgg ggccctatgt gaccgatatc tagccgtcct cgagacttcc
     6181 ctgtgttgca gcgctcattt ccagctgagc cacgcctgcc gggccacctg cccgacccac
     6241 atgggagaga aaagctgcca cctttttagg agccagcgcc accttgggac aaaaagggaa
     6301 acctagtaat gccatcacat ggaggacgag gcccagctca gctgggccag agcccagaag
     6361 tgccacctca tcataattca agtgttcttc cacacagcgt tgcccccaca accacgccgg
     6421 acgtgccccc tcgccacctt ttccagacga cttcttagaa gagatttcat ttatttgtac
     6481 atcttttgca ctttcctatt gaagacttga acacgtttgt cttgataaaa gttggatgac
     6541 gtatggaaga ttcgaacctg cagcactgat gtctctttac cgatgggttc cagacccaag
     6601 gtagtcctgg cactgccctg tggactcagc ccagctgggg aggacatggc gcccggtgcc
     6661 ctaggagccc tcagtgtccc ctacctgacc tgtctgcacc tgtgtgacag ccccttctga
     6721 tttggccccc tgcccgcttg gagcctccca gcaccagaca gggcaggttt ggggagccgc
     6781 tgtccagccc tatggtgaga ccctggctga catttcccct ccttcctgcc aaggctggag
     6841 gccgccaggt gtttgctctc tccttgtggg gaggtggatc ctctgccagc aggtgtctgt
     6901 ccccggccca gcgcatcttg aaggccctgg actctgttac cagtgggggg cctgtggccc
     6961 cggctcttca cagcaggcag aagcgtatga tcccagggag ggtgggctgg ggggggggca
     7021 gcacttgcct cggagccact gctgcgttgg gtttgccttc tgccagaggt gtggttaagg
     7081 gcagtgatag ctgcgcccca gcaaggaggg tcatgggccc tgagctggcc caggagaccc
     7141 tggccctgcc ctgtgccctg ggatgcctac cctgcccacc tccggaggtg gaaccgggct
     7201 gcccctgggg gcctggactt gtcccattcc cctgtccctg gaaaggcctt cccgggggct
     7261 ttttgcctga ggctgcactc ttggaaggcg tggggagagt tctgcctgga gggggactgg
     7321 acccagtgcc ctctgcagac ccctcccagc cgcagcacca agggcttgct gccttgtttc
     7381 ctgccaggag cgccctgaca tcccaaaacc atcttcccac atgtgggttg ttggaatccc
     7441 acccactccc ccggaccctg cccctccaag ttggggacgg agatggggag caggtcaggc
     7501 cctccctagg cctgtttgtg tgtgatcctt ctcctggccc caggtcgacc tggaaggcgt
     7561 ttctgagtct tgtactggga ccttcccagg gaaggtgcct tggggtgcag aatcctggga
     7621 atgttaggaa agggctgcgt gaaaaaccca gctgggcctg gggtggaggt gcacacgggc
     7681 tggggctcag ctccttggag agggcctgcc tgggactgag ggggccaggg ctgggtcagg
     7741 attcaccagc ttgtgttgca gacactggag cgattgcctg gccctcccta agccctatat
     7801 gcctcactca taatgaggct gtctctgaac cccggagggc gggacctaca agtccttcct
     7861 cttggcgcat tcccacactc cagtctggat ccaggtctgc aaggccagcc cgaccctgac
     7921 actgcacgtg ggcgtagtgg ggagcaccct ggagtggaga tgattgtcag cgggctcaca
     7981 tgttatggtt catccacatg cgtgttgtgt gctctgcggt gcctcctggc aaagggtcct
     8041 ggctcgggtc agaagctcac tcccgtgccc tcgtccccaa acaagcagtg gcaagcactg
     8101 gggttggccc tcgttgggag cagtgcccac cttccttggc ccacagcaga taggtcccga
     8161 gcagcaggac tggaggcctg tggcggtcag ggcaggggtc tgtgtctcca ccccaagggc
     8221 tgatggtccc tcccctgtgc gccttcctct caggcaggct tgctctgtcc cttcctctct
     8281 gcttccctcc caggcctcgc cagggctccc ttctcctctg cctggttaga ctcgggcacc
     8341 caggaaagcc tggccagggc tcctttgggc ctgggccccc tgcactgcct ggtccagaag
     8401 gggtggtgct gtccgtggcc agcaccccgg ggcccgggag gggtgggcta ctagagtcag
     8461 agcgggtttg gggctgacca gtttgggaga ggagaaaaga tctgagaatg tccttcttgg
     8521 tttgtcagtc atctctgcca aaagtggtga tggtggtgtc cgtatgtttg gcgtctttgg
     8581 gctgggtttg gtttttgctg ctggtagaat cagggtcctc gggcatgaac gcgagcccaa
     8641 agtgccagtc tgcgattgga aatttccagc cactttaagc cagtgctgag tagggcttct
     8701 gcagagccat gtttgagcca aggtcttgga aggcattgcc ccatgggctc aggtgactcg
     8761 gggtggagtg agcacgtctg cagggccctc tcatacacgc ctgaggcaga agcagcgtcc
     8821 cccgtgaaag ccaccttccg aagctcctgc gttttttgca aacttggctt cccccagggg
     8881 caggctggac tttccctgcc ccctatgatt gaagtcctcc tgcttttggg ggctgccttc
     8941 ccagagtccc ccgggtgctc ccctgccgag gtcaggagct gaccaagcct tggcccggtg
     9001 acacctgcag ccctcactcc tgtcatccca ggacacttga ggcccaagga ggtggagtgg
     9061 agagtgggct cgggtacatg ggagccagaa gccagatgga cttggtcaag tgtcggtcac
     9121 ttggagcctc cagtgtgcgt cagggtctgt gggcagggga cagggcgtgg gtgggggccg
     9181 aggctggcac gcccctctgc cctcaccgtc ttggtgacct ggcctcggcc cctcccccaa
     9241 gtctcttctg tgcaaggccc gcctcggcct cggcagctgg ttcctgtcct gttttctgtg
     9301 tctgaaagtt tacaggttgt ggtgcatcag cccaaactca ctggcgttgt gttttttttt
     9361 tctttaattt tcagattttt tttttaaaca aagtattttt ttaggtgcga taacccagaa
     9421 agggcccgtt gggtgtgtgt gtgtgtcctg aactcctcaa gcagcgattg gagcccaagc
     9481 acccctggag aggaagggag ggtccccact ggcccgtggg gtctgagttc aggggtgtgg
     9541 agggagcaga ctccaccggc ccaggcccag ctaagagggg gccgacccct ttccccaggc
     9601 acagccccag gctggcaaag ggagggccct gggctgggtg caaggcgcgc caggagtccc
     9661 agccagggtg gccccggcgg gggcgggtcc agctttggaa gccaggctcc cctgtgagcc
     9721 gtggcttgtc tggtcttcgc ccacgggagg ctggacagag gctgtagcca acacaatcac
     9781 ctttactttg tactctgtgt gtatgttttg gttttctgtg ttttaataaa tcctttggga
     9841 aaggatttaa
//
