LOCUS       NM_001363606            6461 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4),
            transcript variant 3, mRNA.
ACCESSION   NM_001363606 XM_006718962
VERSION     NM_001363606.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Mamontova,V., Trifault,B., Gribling-Burrer,A.S., Bohn,P., Boten,L.,
            Preckwinkel,P., Gallant,P., Solvie,D., Ade,C.P., Papadopoulos,D.,
            Eilers,M., Gutschner,T., Smyth,R.P. and Burger,K.
  TITLE     NEAT1 promotes genome stability via m6A methylation-dependent
            regulation of CHD4
  JOURNAL   Genes Dev 38 (17-20), 915-930 (2024)
   PUBMED   39362776
  REMARK    GeneRIF: NEAT1 promotes genome stability via m[6]A
            methylation-dependent regulation of CHD4.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Zhang,Q., Zhu,F., Tong,Y., Shi,D. and Zhang,J.
  TITLE     CHD4 R975H mutant activates tumorigenic pathways and promotes
            stemness and M2-like macrophage polarization in endometrial cancer
  JOURNAL   Sci Rep 14 (1), 18617 (2024)
   PUBMED   39127769
  REMARK    GeneRIF: CHD4 R975H mutant activates tumorigenic pathways and
            promotes stemness and M2-like macrophage polarization in
            endometrial cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6461)
  AUTHORS   Hino,Y., Kohashi,K., Tamaki,A., Kawakubo,N., Hamada,H.,
            Fukuhara,M., Shibui,Y., Tajiri,T. and Oda,Y.
  TITLE     FOXM1 and CHD4 expression is associated with chemoresistance in
            hepatoblastoma
  JOURNAL   Pathol Res Pract 258, 155348 (2024)
   PUBMED   38761648
  REMARK    GeneRIF: FOXM1 and CHD4 expression is associated with
            chemoresistance in hepatoblastoma.
REFERENCE   4  (bases 1 to 6461)
  AUTHORS   Shi,W., Wasson,L.K., Dorr,K.M., Robbe,Z.L., Wilczewski,C.M.,
            Hepperla,A.J., Davis,I.J., Seidman,C.E., Seidman,J.G. and
            Conlon,F.L.
  TITLE     CHD4 and SMYD1 repress common transcriptional programs in the
            developing heart
  JOURNAL   Development 151 (8) (2024)
   PUBMED   38619323
  REMARK    GeneRIF: CHD4 and SMYD1 repress common transcriptional programs in
            the developing heart.
REFERENCE   5  (bases 1 to 6461)
  AUTHORS   Le Gallo,M., O'Hara,A.J., Rudd,M.L., Urick,M.E., Hansen,N.F.,
            O'Neil,N.J., Price,J.C., Zhang,S., England,B.M., Godwin,A.K.,
            Sgroi,D.C., Hieter,P., Mullikin,J.C., Merino,M.J. and Bell,D.W.
  CONSRTM   NIH Intramural Sequencing Center (NISC) Comparative Sequencing
            Program
  TITLE     Exome sequencing of serous endometrial tumors identifies recurrent
            somatic mutations in chromatin-remodeling and ubiquitin ligase
            complex genes
  JOURNAL   Nat Genet 44 (12), 1310-1315 (2012)
   PUBMED   23104009
REFERENCE   6  (bases 1 to 6461)
  AUTHORS   Woodage,T., Basrai,M.A., Baxevanis,A.D., Hieter,P. and Collins,F.S.
  TITLE     Characterization of the CHD family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (21), 11472-11477 (1997)
   PUBMED   9326634
REFERENCE   7  (bases 1 to 6461)
  AUTHORS   Seelig,H.P., Renz,M., Targoff,I.N., Ge,Q. and Frank,M.B.
  TITLE     Two forms of the major antigenic protein of the
            dermatomyositis-specific Mi-2 autoantigen
  JOURNAL   Arthritis Rheum 39 (10), 1769-1771 (1996)
   PUBMED   8843877
REFERENCE   8  (bases 1 to 6461)
  AUTHORS   Seelig,H.P., Moosbrugger,I., Ehrfeld,H., Fink,T., Renz,M. and
            Genth,E.
  TITLE     The major dermatomyositis-specific Mi-2 autoantigen is a presumed
            helicase involved in transcriptional activation
  JOURNAL   Arthritis Rheum 38 (10), 1389-1399 (1995)
   PUBMED   7575689
REFERENCE   9  (bases 1 to 6461)
  AUTHORS   Ge,Q., Nilasena,D.S., O'Brien,C.A., Frank,M.B. and Targoff,I.N.
  TITLE     Molecular analysis of a major antigenic region of the 240-kD
            protein of Mi-2 autoantigen
  JOURNAL   J Clin Invest 96 (4), 1730-1737 (1995)
   PUBMED   7560064
REFERENCE   10 (bases 1 to 6461)
  AUTHORS   Weiss,K. and Lachlan,K.
  TITLE     CHD4 Neurodevelopmental Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   32881470
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006064.10.
            
            On Jun 2, 2019 this sequence version replaced NM_001363606.1.
            
            Summary: The product of this gene belongs to the SNF2/RAD54
            helicase family. It represents the main component of the nucleosome
            remodeling and deacetylase complex and plays an important role in
            epigenetic transcriptional repression. Patients with
            dermatomyositis develop antibodies against this protein. Somatic
            mutations in this gene are associated with serous endometrial
            tumors. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jul
            2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.258631.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AC006064.10        164381-164460       c
            81-258              AC006064.10        163355-163532       c
            259-380             AC006064.10        159457-159578       c
            381-596             AC006064.10        159041-159256       c
            597-715             AC006064.10        158729-158847       c
            716-957             AC006064.10        158370-158611       c
            958-1085            AC006064.10        158007-158134       c
            1086-1221           AC006064.10        157615-157750       c
            1222-1361           AC006064.10        157298-157437       c
            1362-1601           AC006064.10        156854-157093       c
            1602-1805           AC006064.10        155303-155506       c
            1806-2011           AC006064.10        154975-155180       c
            2012-2143           AC006064.10        153087-153218       c
            2144-2240           AC006064.10        152415-152511       c
            2241-2432           AC006064.10        151540-151731       c
            2433-2633           AC006064.10        150497-150697       c
            2634-2771           AC006064.10        150172-150309       c
            2772-2893           AC006064.10        149777-149898       c
            2894-3067           AC006064.10        149474-149647       c
            3068-3209           AC006064.10        148997-149138       c
            3210-3341           AC006064.10        148775-148906       c
            3342-3459           AC006064.10        148547-148664       c
            3460-3584           AC006064.10        145379-145503       c
            3585-3822           AC006064.10        144793-145030       c
            3823-3998           AC006064.10        144465-144640       c
            3999-4179           AC006064.10        140279-140459       c
            4180-4266           AC006064.10        140108-140194       c
            4267-4355           AC006064.10        139929-140017       c
            4356-4489           AC006064.10        139696-139829       c
            4490-4634           AC006064.10        139218-139362       c
            4635-4800           AC006064.10        138730-138895       c
            4801-4898           AC006064.10        138372-138469       c
            4899-5034           AC006064.10        138119-138254       c
            5035-5106           AC006064.10        135927-135998       c
            5107-5244           AC006064.10        135490-135627       c
            5245-5353           AC006064.10        135110-135218       c
            5354-5486           AC006064.10        134866-134998       c
            5487-5685           AC006064.10        130152-130350       c
            5686-5849           AC006064.10        127950-128113       c
            5850-6461           AC006064.10        127163-127774       c
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..6461
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /note="chromodomain helicase DNA binding protein 4"
                     /db_xref="GeneID:1108"
                     /db_xref="HGNC:HGNC:1919"
                     /db_xref="MIM:603277"
     exon            1..80
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            81..258
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..5867
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /EC_number="3.6.4.12"
                     /note="isoform 3 is encoded by transcript variant 3; Mi-2
                     autoantigen 218 kDa protein; ATP-dependent helicase CHD4"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 4
                     isoform 3"
                     /protein_id="NP_001350535.1"
                     /db_xref="CCDS:CCDS86267.1"
                     /db_xref="GeneID:1108"
                     /db_xref="HGNC:HGNC:1919"
                     /db_xref="MIM:603277"
                     /translation="MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLS
                     ETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALR
                     SDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGM
                     EDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPF
                     KGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKG
                     SPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDG
                     STSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAP
                     EGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLC
                     CDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPR
                     PPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKND
                     MDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGH
                     VHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLR
                     KLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEM
                     GLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKD
                     SRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWAC
                     LIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNL
                     EGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKY
                     YKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGS
                     ALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGIT
                     GNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSR
                     AHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDD
                     ILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNE
                     YLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNL
                     GKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKG
                     LRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLR
                     GKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQ
                     EFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIK
                     IEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEV
                     KERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGET
                     PKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWL
                     LAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVI
                     EEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKG
                     ILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQ
                     QQ"
     exon            259..380
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            381..596
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            597..715
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            716..957
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1085
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1221
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1361
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1601
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1805
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..2011
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2143
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2240
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2432
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2633
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2634..2771
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2772..2893
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..3067
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3068..3209
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3210..3341
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3459
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3584
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3585..3822
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3823..3998
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3999..4179
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4180..4266
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4355
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4489
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4490..4634
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4635..4800
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4801..4898
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4899..5034
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5035..5106
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5107..5244
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5245..5353
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5354..5486
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5487..5685
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5686..5849
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5850..6461
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6439..6444
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /note="hexamer: AATAAA"
     polyA_site      6461
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /note="major polyA site"
ORIGIN      
        1 cccccttcgg ccgcggcgag gaggagccgg agcgggagtg acaccgagcc ggacccagcg
       61 cgacctgcgg cggctccggg tgactcgggc cagtgtagag gtcctcaggc cgccggcagg
      121 agcagctggg ccaattccct ggccgggagc ggaaggggat ggcgtcgggc ctgggctccc
      181 cgtccccctg ctcggcgggc agtgaggagg aggatatgga tgcacttttg aacaacagcc
      241 tgcccccacc ccacccagaa aatgaagagg acccagaaga ggatttgtca gaaacagaga
      301 ctccaaagct caagaagaag aaaaagccta agaaacctcg ggaccctaaa atccctaaga
      361 gcaagcgcca aaaaaaggag cgtatgctct tatgccggca gctgggggac agctctgggg
      421 aggggccaga gtttgtggag gaggaggaag aggtggctct gcgctcagac agtgagggca
      481 gcgactatac tcctggcaag aagaagaaga agaagcttgg acctaagaaa gagaagaaga
      541 gcaaatccaa gcggaaggag gaggaggagg aggaggatga tgatgatgat tcaaaggagc
      601 ctaaatcatc tgctcagctc ctggaagact ggggcatgga agacattgac cacgtgttct
      661 cagaggagga ttatcgaacc ctcaccaact acaaggcctt cagccagttt gtcagacccc
      721 tcattgctgc caaaaatccc aagattgctg tctccaagat gatgatggtt ttgggtgcaa
      781 aatggcggga gttcagtacc aataacccct tcaaaggcag ttctggggca tcagtggcag
      841 ctgcggcagc agcagcggta gctgtggtgg agagcatggt gacagccact gaggttgcac
      901 caccacctcc ccctgtggag gtgcctatcc gcaaggccaa gaccaaggag ggcaaaggtc
      961 ccaatgctcg gaggaagccc aagggcagcc ctcgtgtacc tgatgccaag aagcctaaac
     1021 ccaagaaagt agctcccctg aaaatcaagc tgggaggttt tggttccaag cgtaagagat
     1081 cctcgagtga ggatgatgac ttagatgtgg aatctgactt cgatgatgcc agtatcaata
     1141 gctattctgt ttctgatggt tccaccagcc gtagtagccg cagccgcaag aaactccgaa
     1201 ccactaaaaa gaaaaagaaa gaccaccagg actattgcga ggtgtgccag caaggcggtg
     1261 agatcatcct gtgtgatacc tgtccccgtg cttaccacat ggtctgcctg gatcccgaca
     1321 tggagaaggc tcccgagggc aagtggagct gcccacactg cgagaaggaa ggcatccagt
     1381 gggaagctaa agaggacaat tcggagggtg aggagatcct ggaagaggtt gggggagacc
     1441 tcgaagagga ggatgaccac catatggaat tctgtcgggt ctgcaaggat ggtggggaac
     1501 tgctctgctg tgatacctgt ccttcttcct accacatcca ctgcctgaat cccccacttc
     1561 cagagatccc caacggtgaa tggctctgtc cccgttgtac gtgtccagct ctgaagggca
     1621 aagtgcagaa gatcctaatc tggaagtggg gtcagccacc atctcccaca ccagtgcctc
     1681 ggcctccaga tgctgatccc aacacgccct ccccaaagcc cttggagggg cggccagagc
     1741 ggcagttctt tgtgaaatgg caaggcatgt cttactggca ctgctcctgg gtttctgaac
     1801 tgcagctgga gctgcactgt caggtgatgt tccgaaacta tcagcggaag aatgatatgg
     1861 atgagccacc ttctggggac tttggtggtg atgaagagaa aagccgaaag cgaaagaaca
     1921 aggaccctaa atttgcagag atggaggaac gcttctatcg ctatgggata aaacccgagt
     1981 ggatgatgat ccaccgaatc ctcaaccaca gtgtggacaa gaagggccac gtccactact
     2041 tgatcaagtg gcgggactta ccttacgatc aggcttcttg ggagagtgag gatgtggaga
     2101 tccaggatta cgacctgttc aagcagagct attggaatca cagggagtta atgaggggtg
     2161 aggaaggccg accaggcaag aagctcaaga aggtgaagct tcggaagttg gagaggcctc
     2221 cagaaacgcc aacagttgat ccaacagtga agtatgagcg acagccagag tacctggatg
     2281 ctacaggtgg aaccctgcac ccctatcaaa tggagggcct gaattggttg cgcttctcct
     2341 gggctcaggg cactgacacc atcttggctg atgagatggg ccttgggaaa actgtacaga
     2401 cagcagtctt cctgtattcc ctttacaagg agggtcattc caaaggcccc ttcctagtga
     2461 gcgcccctct ttctaccatc atcaactggg agcgggagtt tgaaatgtgg gctccagaca
     2521 tgtatgtcgt aacctatgtg ggtgacaagg acagccgtgc catcatccga gagaatgagt
     2581 tctcctttga agacaatgcc attcgtggtg gcaagaaggc ctcccgcatg aagaaagagg
     2641 catctgtgaa attccatgtg ctgctgacat cctatgaatt gatcaccatt gacatggcta
     2701 ttttgggctc tattgattgg gcctgcctca tcgtggatga agcccatcgg ctgaagaaca
     2761 atcagtctaa gttcttccgg gtattgaatg gttactcact ccagcacaag ctgttgctga
     2821 ctgggacacc attacaaaac aatctggaag agttgtttca tctgctcaac tttctcaccc
     2881 ccgagaggtt ccacaatttg gaaggttttt tggaggagtt tgctgacatt gccaaggagg
     2941 accagataaa aaaactgcat gacatgctgg ggccgcacat gttgcggcgg ctcaaagccg
     3001 atgtgttcaa gaacatgccc tccaagacag aactaattgt gcgtgtggag ctgagcccta
     3061 tgcagaagaa atactacaag tacatcctca ctcgaaattt tgaagcactc aatgcccgag
     3121 gtggtggcaa ccaggtgtct ctgctgaatg tggtgatgga tcttaagaag tgctgcaacc
     3181 atccatacct cttccctgtg gctgcaatgg aagctcctaa gatgcctaat ggcatgtatg
     3241 atggcagtgc cctaatcaga gcatctggga aattattgct gctgcagaaa atgctcaaga
     3301 accttaagga gggtgggcat cgtgtactca tcttttccca gatgaccaag atgctagacc
     3361 tgctagagga tttcttggaa catgaaggtt ataaatacga acgcatcgat ggtggaatca
     3421 ctgggaacat gcggcaagag gccattgacc gcttcaatgc accgggtgct cagcagttct
     3481 gcttcttgct ttccactcga gctgggggcc ttggaatcaa tctggccact gctgacacag
     3541 ttattatcta tgactctgac tggaaccccc ataatgacat tcaggccttt agcagagctc
     3601 accggattgg gcaaaataaa aaggtaatga tctaccggtt tgtgacccgt gcgtcagtgg
     3661 aggagcgcat cacgcaggtg gcaaagaaga aaatgatgct gacgcatcta gtggtgcggc
     3721 ctgggctggg ctccaagact ggatctatgt ccaaacagga gcttgatgat atcctcaaat
     3781 ttggcactga ggaactattc aaggatgaag ccactgatgg aggaggagac aacaaagagg
     3841 gagaagatag cagtgttatc cactacgatg ataaggccat tgaacggctg ctagaccgta
     3901 accaggatga gactgaagac acagaattgc agggcatgaa tgaatatttg agctcattca
     3961 aagtggccca gtatgtggta cgggaagaag aaatggggga ggaagaggag gtagaacggg
     4021 aaatcattaa acaggaagaa agtgtggatc ctgactactg ggagaaattg ctgcggcacc
     4081 attatgagca gcagcaagaa gatctagccc gaaatctggg caaaggaaaa agaatccgta
     4141 aacaggtcaa ctacaatgat ggctcccagg aggaccgaga ttggcaggac gaccagtccg
     4201 acaaccagtc cgattactca gtggcttcag aggaaggtga tgaagacttt gatgaacgtt
     4261 cagaagctcc ccgtaggccc agtcgtaagg gcctgcggaa tgataaagat aagccattgc
     4321 ctcctctgtt ggcccgtgtt ggtgggaata ttgaagtact tggttttaat gctcgtcagc
     4381 gaaaagcctt tcttaatgca attatgcgat atggtatgcc acctcaggat gcttttacta
     4441 cccagtggct tgtaagagac ctgcgaggca aatcagagaa agagttcaag gcatatgtct
     4501 ctcttttcat gcggcattta tgtgagccgg gggcagatgg ggctgagacc tttgctgatg
     4561 gtgtcccccg agaaggcctg tctcgccagc atgtccttac tagaattggt gttatgtctt
     4621 tgattcgcaa gaaggttcag gagtttgaac atgttaatgg gcgctggagc atgcctgaac
     4681 tggctgaggt ggaggaaaac aagaagatgt cccagccagg gtcaccctcc ccaaaaactc
     4741 ctacaccctc cactccaggg gacacgcagc ccaacactcc tgcacctgtc ccacctgctg
     4801 aagatgggat aaaaatagag gaaaatagcc tcaaagaaga agagagcata gaaggagaaa
     4861 aggaggttaa atctacagcc cctgagactg ccattgagtg tacacaggcc cctgcccctg
     4921 cctcagagga tgaaaaggtc gttgttgaac cccctgaggg agaggagaaa gtggaaaagg
     4981 cagaggtgaa ggagagaaca gaggaaccta tggagacaga gcccaaaggt aaaggtgctg
     5041 ctgatgtaga gaaggtggag gaaaagtcag caatagatct gacccctatt gtggtagaag
     5101 acaaagaaga gaagaaagaa gaagaagaga aaaaagaggt gatgcttcag aatggagaga
     5161 cccccaagga cctgaatgat gagaaacaga agaaaaatat taaacaacgt ttcatgttta
     5221 acattgcaga tggtggtttt actgagttgc actccctttg gcagaatgaa gagcgggcag
     5281 ccacagttac caagaagact tatgagatct ggcatcgacg gcatgactac tggctgctag
     5341 ccggcattat aaaccatggc tatgcccggt ggcaagacat ccagaatgac ccacgctatg
     5401 ccatcctcaa tgagcctttc aagggtgaaa tgaaccgtgg caatttctta gagatcaaga
     5461 ataaatttct agctcgaagg tttaagctct tagaacaagc tctggtgatt gaggaacagc
     5521 tgcgccgggc tgcttacttg aacatgtcag aagacccttc tcacccttcc atggccctca
     5581 acacccgctt tgctgaggtg gagtgtttgg cggaaagtca tcagcacctg tccaaggagt
     5641 caatggcagg aaacaagcca gccaatgcag tcctgcacaa aggtattctg aaacagctgg
     5701 aagaactgct gagtgacatg aaagctgatg tgactcgact cccagctacc attgcccgaa
     5761 ttcccccagt tgctgtgagg ttacagatgt cagagcgtaa cattctcagc cgcctggcaa
     5821 accgggcacc cgaacctacc ccacagcagg tagcccagca gcagtgaaga tgcagactga
     5881 taccacctcc accgctgagc agtgaccttc ctcactttct cttgtcccag cttctcccct
     5941 gggggcctga gagaccctca ccttccttct gcccatcttc catgttgtaa aggaacagcc
     6001 ccagtgcact gggggagggg agggagtgag gggcagtggt gcccttcctg cagaagagac
     6061 atgcagcagt agcgctggcg ccatctgcag gagctggcgg gctggccttc tggaccctgg
     6121 cttctcccca ctgtaacgcc tgttacacac aaactgttgt gggttcctgc caggcttgaa
     6181 gaaaatgatc tgaatttttt cctccttttg gttttatttt gttggtttat tttgtgtttt
     6241 cttttctcct ttttgggggg tattcagagt gggctgggcc cctgggcgag acacagctac
     6301 ctctgttggc atctttttaa taccaggaac ccagcggctc tagccactga gcggctaaat
     6361 gaaataaagt ggaaaaaaaa aaaaaggaaa aaaccaaaag cataaaaaac cacagcaaat
     6421 ttcttgatga aaattgaaaa taaaagtttc cttgtatttt a
//
