LOCUS       NM_001273               6491 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4),
            transcript variant 1, mRNA.
ACCESSION   NM_001273
VERSION     NM_001273.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Mamontova,V., Trifault,B., Gribling-Burrer,A.S., Bohn,P., Boten,L.,
            Preckwinkel,P., Gallant,P., Solvie,D., Ade,C.P., Papadopoulos,D.,
            Eilers,M., Gutschner,T., Smyth,R.P. and Burger,K.
  TITLE     NEAT1 promotes genome stability via m6A methylation-dependent
            regulation of CHD4
  JOURNAL   Genes Dev 38 (17-20), 915-930 (2024)
   PUBMED   39362776
  REMARK    GeneRIF: NEAT1 promotes genome stability via m[6]A
            methylation-dependent regulation of CHD4.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Zhang,Q., Zhu,F., Tong,Y., Shi,D. and Zhang,J.
  TITLE     CHD4 R975H mutant activates tumorigenic pathways and promotes
            stemness and M2-like macrophage polarization in endometrial cancer
  JOURNAL   Sci Rep 14 (1), 18617 (2024)
   PUBMED   39127769
  REMARK    GeneRIF: CHD4 R975H mutant activates tumorigenic pathways and
            promotes stemness and M2-like macrophage polarization in
            endometrial cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6491)
  AUTHORS   Hino,Y., Kohashi,K., Tamaki,A., Kawakubo,N., Hamada,H.,
            Fukuhara,M., Shibui,Y., Tajiri,T. and Oda,Y.
  TITLE     FOXM1 and CHD4 expression is associated with chemoresistance in
            hepatoblastoma
  JOURNAL   Pathol Res Pract 258, 155348 (2024)
   PUBMED   38761648
  REMARK    GeneRIF: FOXM1 and CHD4 expression is associated with
            chemoresistance in hepatoblastoma.
REFERENCE   4  (bases 1 to 6491)
  AUTHORS   Shi,W., Wasson,L.K., Dorr,K.M., Robbe,Z.L., Wilczewski,C.M.,
            Hepperla,A.J., Davis,I.J., Seidman,C.E., Seidman,J.G. and
            Conlon,F.L.
  TITLE     CHD4 and SMYD1 repress common transcriptional programs in the
            developing heart
  JOURNAL   Development 151 (8) (2024)
   PUBMED   38619323
  REMARK    GeneRIF: CHD4 and SMYD1 repress common transcriptional programs in
            the developing heart.
REFERENCE   5  (bases 1 to 6491)
  AUTHORS   Le Gallo,M., O'Hara,A.J., Rudd,M.L., Urick,M.E., Hansen,N.F.,
            O'Neil,N.J., Price,J.C., Zhang,S., England,B.M., Godwin,A.K.,
            Sgroi,D.C., Hieter,P., Mullikin,J.C., Merino,M.J. and Bell,D.W.
  CONSRTM   NIH Intramural Sequencing Center (NISC) Comparative Sequencing
            Program
  TITLE     Exome sequencing of serous endometrial tumors identifies recurrent
            somatic mutations in chromatin-remodeling and ubiquitin ligase
            complex genes
  JOURNAL   Nat Genet 44 (12), 1310-1315 (2012)
   PUBMED   23104009
REFERENCE   6  (bases 1 to 6491)
  AUTHORS   Woodage,T., Basrai,M.A., Baxevanis,A.D., Hieter,P. and Collins,F.S.
  TITLE     Characterization of the CHD family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (21), 11472-11477 (1997)
   PUBMED   9326634
REFERENCE   7  (bases 1 to 6491)
  AUTHORS   Seelig,H.P., Renz,M., Targoff,I.N., Ge,Q. and Frank,M.B.
  TITLE     Two forms of the major antigenic protein of the
            dermatomyositis-specific Mi-2 autoantigen
  JOURNAL   Arthritis Rheum 39 (10), 1769-1771 (1996)
   PUBMED   8843877
REFERENCE   8  (bases 1 to 6491)
  AUTHORS   Seelig,H.P., Moosbrugger,I., Ehrfeld,H., Fink,T., Renz,M. and
            Genth,E.
  TITLE     The major dermatomyositis-specific Mi-2 autoantigen is a presumed
            helicase involved in transcriptional activation
  JOURNAL   Arthritis Rheum 38 (10), 1389-1399 (1995)
   PUBMED   7575689
REFERENCE   9  (bases 1 to 6491)
  AUTHORS   Ge,Q., Nilasena,D.S., O'Brien,C.A., Frank,M.B. and Targoff,I.N.
  TITLE     Molecular analysis of a major antigenic region of the 240-kD
            protein of Mi-2 autoantigen
  JOURNAL   J Clin Invest 96 (4), 1730-1737 (1995)
   PUBMED   7560064
REFERENCE   10 (bases 1 to 6491)
  AUTHORS   Weiss,K. and Lachlan,K.
  TITLE     CHD4 Neurodevelopmental Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   32881470
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP234473.1, X86691.1,
            AC006064.10 and BC038596.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001273.4.
            
            Summary: The product of this gene belongs to the SNF2/RAD54
            helicase family. It represents the main component of the nucleosome
            remodeling and deacetylase complex and plays an important role in
            epigenetic transcriptional repression. Patients with
            dermatomyositis develop antibodies against this protein. Somatic
            mutations in this gene are associated with serous endometrial
            tumors. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jul
            2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X86691.1, SRR11853563.6734.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000544040.7/ ENSP00000440542.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                BP234473.1         48-116
            70-574              X86691.1           1-505
            575-575             AC006064.10        159062-159062       c
            576-6415            X86691.1           507-6346
            6416-6485           X86691.1           6348-6417
            6486-6491           BC038596.2         6542-6547
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..6491
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /note="chromodomain helicase DNA binding protein 4"
                     /db_xref="GeneID:1108"
                     /db_xref="HGNC:HGNC:1919"
                     /db_xref="MIM:603277"
     exon            1..80
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            81..258
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..5897
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; Mi-2
                     autoantigen 218 kDa protein; ATP-dependent helicase CHD4"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 4
                     isoform 1"
                     /protein_id="NP_001264.2"
                     /db_xref="CCDS:CCDS8552.1"
                     /db_xref="GeneID:1108"
                     /db_xref="HGNC:HGNC:1919"
                     /db_xref="MIM:603277"
                     /translation="MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLS
                     ETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALR
                     SDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGM
                     EDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPF
                     KGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKG
                     SPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDG
                     STSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAY
                     HMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHME
                     FCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIW
                     KWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELH
                     CQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMI
                     HRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEE
                     GRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFS
                     WAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWA
                     PDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELIT
                     IDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFH
                     LLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTEL
                     IVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAM
                     EAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEH
                     EGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDS
                     DWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLG
                     SKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQ
                     DETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRH
                     HYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFD
                     ERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQ
                     DAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLT
                     RIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPN
                     TPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVE
                     PPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEE
                     KKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTY
                     EIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLAR
                     RFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAG
                     NKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRA
                     PEPTPQQVAQQQ"
     exon            259..380
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            381..596
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            597..715
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            716..957
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1085
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1221
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1400
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1640
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1844
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2050
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..2182
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2279
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2280..2471
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2472..2672
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..2810
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2811..2932
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            2933..3106
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3107..3248
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3249..3380
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3381..3498
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3499..3623
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3624..3861
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..4037
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4038..4218
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4219..4305
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4306..4394
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4395..4528
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4529..4673
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4674..4839
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4840..4937
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            4938..5067
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5068..5139
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5140..5277
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5278..5386
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5387..5519
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5520..5715
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5716..5879
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     exon            5880..6491
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6469..6474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /note="hexamer: AATAAA"
     polyA_site      6491
                     /gene="CHD4"
                     /gene_synonym="CHD-4; Mi-2b; Mi2-BETA; SIHIWES"
                     /note="major polyA site"
ORIGIN      
        1 cccccttcgg ccgcggcgag gaggagccgg agcgggagtg acaccgagcc ggacccagcg
       61 cgacctgcgg cggctccggg tgactcgggc cagtgtagag gtcctcaggc cgccggcagg
      121 agcagctggg ccaattccct ggccgggagc ggaaggggat ggcgtcgggc ctgggctccc
      181 cgtccccctg ctcggcgggc agtgaggagg aggatatgga tgcacttttg aacaacagcc
      241 tgcccccacc ccacccagaa aatgaagagg acccagaaga ggatttgtca gaaacagaga
      301 ctccaaagct caagaagaag aaaaagccta agaaacctcg ggaccctaaa atccctaaga
      361 gcaagcgcca aaaaaaggag cgtatgctct tatgccggca gctgggggac agctctgggg
      421 aggggccaga gtttgtggag gaggaggaag aggtggctct gcgctcagac agtgagggca
      481 gcgactatac tcctggcaag aagaagaaga agaagcttgg acctaagaaa gagaagaaga
      541 gcaaatccaa gcggaaggag gaggaggagg aggaggatga tgatgatgat tcaaaggagc
      601 ctaaatcatc tgctcagctc ctggaagact ggggcatgga agacattgac cacgtgttct
      661 cagaggagga ttatcgaacc ctcaccaact acaaggcctt cagccagttt gtcagacccc
      721 tcattgctgc caaaaatccc aagattgctg tctccaagat gatgatggtt ttgggtgcaa
      781 aatggcggga gttcagtacc aataacccct tcaaaggcag ttctggggca tcagtggcag
      841 ctgcggcagc agcagcggta gctgtggtgg agagcatggt gacagccact gaggttgcac
      901 caccacctcc ccctgtggag gtgcctatcc gcaaggccaa gaccaaggag ggcaaaggtc
      961 ccaatgctcg gaggaagccc aagggcagcc ctcgtgtacc tgatgccaag aagcctaaac
     1021 ccaagaaagt agctcccctg aaaatcaagc tgggaggttt tggttccaag cgtaagagat
     1081 cctcgagtga ggatgatgac ttagatgtgg aatctgactt cgatgatgcc agtatcaata
     1141 gctattctgt ttctgatggt tccaccagcc gtagtagccg cagccgcaag aaactccgaa
     1201 ccactaaaaa gaaaaagaaa ggcgaggagg aggtgactgc tgtggatggt tatgagacag
     1261 accaccagga ctattgcgag gtgtgccagc aaggcggtga gatcatcctg tgtgatacct
     1321 gtccccgtgc ttaccacatg gtctgcctgg atcccgacat ggagaaggct cccgagggca
     1381 agtggagctg cccacactgc gagaaggaag gcatccagtg ggaagctaaa gaggacaatt
     1441 cggagggtga ggagatcctg gaagaggttg ggggagacct cgaagaggag gatgaccacc
     1501 atatggaatt ctgtcgggtc tgcaaggatg gtggggaact gctctgctgt gatacctgtc
     1561 cttcttccta ccacatccac tgcctgaatc ccccacttcc agagatcccc aacggtgaat
     1621 ggctctgtcc ccgttgtacg tgtccagctc tgaagggcaa agtgcagaag atcctaatct
     1681 ggaagtgggg tcagccacca tctcccacac cagtgcctcg gcctccagat gctgatccca
     1741 acacgccctc cccaaagccc ttggaggggc ggccagagcg gcagttcttt gtgaaatggc
     1801 aaggcatgtc ttactggcac tgctcctggg tttctgaact gcagctggag ctgcactgtc
     1861 aggtgatgtt ccgaaactat cagcggaaga atgatatgga tgagccacct tctggggact
     1921 ttggtggtga tgaagagaaa agccgaaagc gaaagaacaa ggaccctaaa tttgcagaga
     1981 tggaggaacg cttctatcgc tatgggataa aacccgagtg gatgatgatc caccgaatcc
     2041 tcaaccacag tgtggacaag aagggccacg tccactactt gatcaagtgg cgggacttac
     2101 cttacgatca ggcttcttgg gagagtgagg atgtggagat ccaggattac gacctgttca
     2161 agcagagcta ttggaatcac agggagttaa tgaggggtga ggaaggccga ccaggcaaga
     2221 agctcaagaa ggtgaagctt cggaagttgg agaggcctcc agaaacgcca acagttgatc
     2281 caacagtgaa gtatgagcga cagccagagt acctggatgc tacaggtgga accctgcacc
     2341 cctatcaaat ggagggcctg aattggttgc gcttctcctg ggctcagggc actgacacca
     2401 tcttggctga tgagatgggc cttgggaaaa ctgtacagac agcagtcttc ctgtattccc
     2461 tttacaagga gggtcattcc aaaggcccct tcctagtgag cgcccctctt tctaccatca
     2521 tcaactggga gcgggagttt gaaatgtggg ctccagacat gtatgtcgta acctatgtgg
     2581 gtgacaagga cagccgtgcc atcatccgag agaatgagtt ctcctttgaa gacaatgcca
     2641 ttcgtggtgg caagaaggcc tcccgcatga agaaagaggc atctgtgaaa ttccatgtgc
     2701 tgctgacatc ctatgaattg atcaccattg acatggctat tttgggctct attgattggg
     2761 cctgcctcat cgtggatgaa gcccatcggc tgaagaacaa tcagtctaag ttcttccggg
     2821 tattgaatgg ttactcactc cagcacaagc tgttgctgac tgggacacca ttacaaaaca
     2881 atctggaaga gttgtttcat ctgctcaact ttctcacccc cgagaggttc cacaatttgg
     2941 aaggtttttt ggaggagttt gctgacattg ccaaggagga ccagataaaa aaactgcatg
     3001 acatgctggg gccgcacatg ttgcggcggc tcaaagccga tgtgttcaag aacatgccct
     3061 ccaagacaga actaattgtg cgtgtggagc tgagccctat gcagaagaaa tactacaagt
     3121 acatcctcac tcgaaatttt gaagcactca atgcccgagg tggtggcaac caggtgtctc
     3181 tgctgaatgt ggtgatggat cttaagaagt gctgcaacca tccatacctc ttccctgtgg
     3241 ctgcaatgga agctcctaag atgcctaatg gcatgtatga tggcagtgcc ctaatcagag
     3301 catctgggaa attattgctg ctgcagaaaa tgctcaagaa ccttaaggag ggtgggcatc
     3361 gtgtactcat cttttcccag atgaccaaga tgctagacct gctagaggat ttcttggaac
     3421 atgaaggtta taaatacgaa cgcatcgatg gtggaatcac tgggaacatg cggcaagagg
     3481 ccattgaccg cttcaatgca ccgggtgctc agcagttctg cttcttgctt tccactcgag
     3541 ctgggggcct tggaatcaat ctggccactg ctgacacagt tattatctat gactctgact
     3601 ggaaccccca taatgacatt caggccttta gcagagctca ccggattggg caaaataaaa
     3661 aggtaatgat ctaccggttt gtgacccgtg cgtcagtgga ggagcgcatc acgcaggtgg
     3721 caaagaagaa aatgatgctg acgcatctag tggtgcggcc tgggctgggc tccaagactg
     3781 gatctatgtc caaacaggag cttgatgata tcctcaaatt tggcactgag gaactattca
     3841 aggatgaagc cactgatgga ggaggagaca acaaagaggg agaagatagc agtgttatcc
     3901 actacgatga taaggccatt gaacggctgc tagaccgtaa ccaggatgag actgaagaca
     3961 cagaattgca gggcatgaat gaatatttga gctcattcaa agtggcccag tatgtggtac
     4021 gggaagaaga aatgggggag gaagaggagg tagaacggga aatcattaaa caggaagaaa
     4081 gtgtggatcc tgactactgg gagaaattgc tgcggcacca ttatgagcag cagcaagaag
     4141 atctagcccg aaatctgggc aaaggaaaaa gaatccgtaa acaggtcaac tacaatgatg
     4201 gctcccagga ggaccgagat tggcaggacg accagtccga caaccagtcc gattactcag
     4261 tggcttcaga ggaaggtgat gaagactttg atgaacgttc agaagctccc cgtaggccca
     4321 gtcgtaaggg cctgcggaat gataaagata agccattgcc tcctctgttg gcccgtgttg
     4381 gtgggaatat tgaagtactt ggttttaatg ctcgtcagcg aaaagccttt cttaatgcaa
     4441 ttatgcgata tggtatgcca cctcaggatg cttttactac ccagtggctt gtaagagacc
     4501 tgcgaggcaa atcagagaaa gagttcaagg catatgtctc tcttttcatg cggcatttat
     4561 gtgagccggg ggcagatggg gctgagacct ttgctgatgg tgtcccccga gaaggcctgt
     4621 ctcgccagca tgtccttact agaattggtg ttatgtcttt gattcgcaag aaggttcagg
     4681 agtttgaaca tgttaatggg cgctggagca tgcctgaact ggctgaggtg gaggaaaaca
     4741 agaagatgtc ccagccaggg tcaccctccc caaaaactcc tacaccctcc actccagggg
     4801 acacgcagcc caacactcct gcacctgtcc cacctgctga agatgggata aaaatagagg
     4861 aaaatagcct caaagaagaa gagagcatag aaggagaaaa ggaggttaaa tctacagccc
     4921 ctgagactgc cattgagtgt acacaggccc ctgcccctgc ctcagaggat gaaaaggtcg
     4981 ttgttgaacc ccctgaggga gaggagaaag tggaaaaggc agaggtgaag gagagaacag
     5041 aggaacctat ggagacagag cccaaaggtg ctgctgatgt agagaaggtg gaggaaaagt
     5101 cagcaataga tctgacccct attgtggtag aagacaaaga agagaagaaa gaagaagaag
     5161 agaaaaaaga ggtgatgctt cagaatggag agacccccaa ggacctgaat gatgagaaac
     5221 agaagaaaaa tattaaacaa cgtttcatgt ttaacattgc agatggtggt tttactgagt
     5281 tgcactccct ttggcagaat gaagagcggg cagccacagt taccaagaag acttatgaga
     5341 tctggcatcg acggcatgac tactggctgc tagccggcat tataaaccat ggctatgccc
     5401 ggtggcaaga catccagaat gacccacgct atgccatcct caatgagcct ttcaagggtg
     5461 aaatgaaccg tggcaatttc ttagagatca agaataaatt tctagctcga aggtttaagc
     5521 tcttagaaca agctctggtg attgaggaac agctgcgccg ggctgcttac ttgaacatgt
     5581 cagaagaccc ttctcaccct tccatggccc tcaacacccg ctttgctgag gtggagtgtt
     5641 tggcggaaag tcatcagcac ctgtccaagg agtcaatggc aggaaacaag ccagccaatg
     5701 cagtcctgca caaagttctg aaacagctgg aagaactgct gagtgacatg aaagctgatg
     5761 tgactcgact cccagctacc attgcccgaa ttcccccagt tgctgtgagg ttacagatgt
     5821 cagagcgtaa cattctcagc cgcctggcaa accgggcacc cgaacctacc ccacagcagg
     5881 tagcccagca gcagtgaaga tgcagactga taccacctcc accgctgagc agtgaccttc
     5941 ctcactttct cttgtcccag cttctcccct gggggcctga gagaccctca ccttccttct
     6001 gcccatcttc catgttgtaa aggaacagcc ccagtgcact gggggagggg agggagtgag
     6061 gggcagtggt gcccttcctg cagaagagac atgcagcagt agcgctggcg ccatctgcag
     6121 gagctggcgg gctggccttc tggaccctgg cttctcccca ctgtaacgcc tgttacacac
     6181 aaactgttgt gggttcctgc caggcttgaa gaaaatgatc tgaatttttt cctccttttg
     6241 gttttatttt gttggtttat tttgtgtttt cttttctcct ttttgggggg tattcagagt
     6301 gggctgggcc cctgggcgag acacagctac ctctgttggc atctttttaa taccaggaac
     6361 ccagcggctc tagccactga gcggctaaat gaaataaagt ggaaaaaaaa aaaaaggaaa
     6421 aaaccaaaag cataaaaaac cacagcaaat ttcttgatga aaattgaaaa taaaagtttc
     6481 cttgtatttt a
//
