LOCUS       NM_001005273            7385 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens chromodomain helicase DNA binding protein 3 (CHD3),
            transcript variant 1, mRNA.
ACCESSION   NM_001005273
VERSION     NM_001005273.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Goldfarb Yaacobi,R. and Sukenik Halevy,R.
  TITLE     A severe neurocognitive phenotype caused by biallelic CHD3 variants
            in two siblings
  JOURNAL   Am J Med Genet A 194 (4), e63503 (2024)
   PUBMED   38116750
  REMARK    GeneRIF: A severe neurocognitive phenotype caused by biallelic CHD3
            variants in two siblings.
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Pascual,P., Tenorio-Castano,J., Mignot,C., Afenjar,A., Arias,P.,
            Gallego-Zazo,N., Parra,A., Miranda,L., Cazalla,M., Silvan,C.,
            Heron,D., Keren,B., Popa,I., Palomares,M., Rikeros,E., Ramos,F.J.,
            Almoguera,B., Ayuso,C., Swafiri,S.T., Barbero,A.I.S.,
            Srinivasan,V.M., Gowda,V.K., Morleo,M., Nigro,V., D'Arrigo,S.,
            Ciaccio,C., Martin Mesa,C., Paumard,B., Guillen,G., Anton,A.T.S.,
            Jimenez,M.D., Seidel,V., Suarez,J., Cormier-Daire,V.,
            Consortium,T.S., Nevado,J. and Lapunzina,P.
  TITLE     Snijders Blok-Campeau Syndrome: Description of 20 Additional
            Individuals with Variants in CHD3 and Literature Review
  JOURNAL   Genes (Basel) 14 (9), 1664 (2023)
   PUBMED   37761804
  REMARK    GeneRIF: Snijders Blok-Campeau Syndrome: Description of 20
            Additional Individuals with Variants in CHD3 and Literature Review.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7385)
  AUTHORS   van der Spek,J., den Hoed,J., Snijders Blok,L., Dingemans,A.J.M.,
            Schijven,D., Nellaker,C., Venselaar,H., Astuti,G.D.N.,
            Barakat,T.S., Bebin,E.M., Beck-Wodl,S., Beunders,G., Brown,N.J.,
            Brunet,T., Brunner,H.G., Campeau,P.M., Cuturilo,G., Gilissen,C.,
            Haack,T.B., Huning,I., Husain,R.A., Kamien,B., Lim,S.C.,
            Lovrecic,L., Magg,J., Maver,A., Miranda,V., Monteil,D.C.,
            Ockeloen,C.W., Pais,L.S., Plaiasu,V., Raiti,L., Richmond,C.,
            Riess,A., Schwaibold,E.M.C., Simon,M.E.H., Spranger,S., Tan,T.Y.,
            Thompson,M.L., de Vries,B.B.A., Wilkins,E.J., Willemsen,M.H.,
            Francks,C., Vissers,L.E.L.M., Fisher,S.E. and Kleefstra,T.
  TITLE     Inherited variants in CHD3 show variable expressivity in Snijders
            Blok-Campeau syndrome
  JOURNAL   Genet Med 24 (6), 1283-1296 (2022)
   PUBMED   35346573
  REMARK    GeneRIF: Inherited variants in CHD3 show variable expressivity in
            Snijders Blok-Campeau syndrome.
REFERENCE   4  (bases 1 to 7385)
  AUTHORS   Hoffmeister,H., Fuchs,A., Komives,E., Groebner-Ferreira,R.,
            Strobl,L., Nazet,J., Heizinger,L., Merkl,R., Dove,S. and Langst,G.
  TITLE     Sequence and functional differences in the ATPase domains of CHD3
            and SNF2H promise potential for selective regulability and
            drugability
  JOURNAL   FEBS J 288 (13), 4000-4023 (2021)
   PUBMED   33403747
  REMARK    GeneRIF: Sequence and functional differences in the ATPase domains
            of CHD3 and SNF2H promise potential for selective regulability and
            drugability.
REFERENCE   5  (bases 1 to 7385)
  AUTHORS   Spruijt,C.G., Grawe,C., Kleinendorst,S.C., Baltissen,M.P.A. and
            Vermeulen,M.
  TITLE     Cross-linking mass spectrometry reveals the structural topology of
            peripheral NuRD subunits relative to the core complex
  JOURNAL   FEBS J 288 (10), 3231-3245 (2021)
   PUBMED   33283408
REFERENCE   6  (bases 1 to 7385)
  AUTHORS   Woodage,T., Basrai,M.A., Baxevanis,A.D., Hieter,P. and Collins,F.S.
  TITLE     Characterization of the CHD family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (21), 11472-11477 (1997)
   PUBMED   9326634
REFERENCE   7  (bases 1 to 7385)
  AUTHORS   Seelig,H.P., Renz,M., Targoff,I.N., Ge,Q. and Frank,M.B.
  TITLE     Two forms of the major antigenic protein of the
            dermatomyositis-specific Mi-2 autoantigen
  JOURNAL   Arthritis Rheum 39 (10), 1769-1771 (1996)
   PUBMED   8843877
REFERENCE   8  (bases 1 to 7385)
  AUTHORS   Seelig,H.P., Moosbrugger,I., Ehrfeld,H., Fink,T., Renz,M. and
            Genth,E.
  TITLE     The major dermatomyositis-specific Mi-2 autoantigen is a presumed
            helicase involved in transcriptional activation
  JOURNAL   Arthritis Rheum 38 (10), 1389-1399 (1995)
   PUBMED   7575689
REFERENCE   9  (bases 1 to 7385)
  AUTHORS   Ge,Q., Nilasena,D.S., O'Brien,C.A., Frank,M.B. and Targoff,I.N.
  TITLE     Molecular analysis of a major antigenic region of the 240-kD
            protein of Mi-2 autoantigen
  JOURNAL   J Clin Invest 96 (4), 1730-1737 (1995)
   PUBMED   7560064
REFERENCE   10 (bases 1 to 7385)
  AUTHORS   Gieser,L. and Swaroop,A.
  TITLE     Expressed sequence tags and chromosomal localization of cDNA clones
            from a subtracted retinal pigment epithelium library
  JOURNAL   Genomics 13 (3), 873-876 (1992)
   PUBMED   1639417
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104581.22, U91543.1,
            AK125928.1 and BC110648.1.
            
            On Aug 31, 2019 this sequence version replaced NM_001005273.2.
            
            Summary: This gene encodes a member of the CHD family of proteins
            which are characterized by the presence of chromo (chromatin
            organization modifier) domains and SNF2-related helicase/ATPase
            domains. This protein is one of the components of a histone
            deacetylase complex referred to as the Mi-2/NuRD complex which
            participates in the remodeling of chromatin by deacetylating
            histones. Chromatin remodeling is essential for many processes
            including transcription. Autoantibodies against this protein are
            found in a subset of patients with dermatomyositis. Three
            alternatively spliced transcripts encoding different isoforms have
            been described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variant 1 encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U91543.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330494.12/ ENSP00000332628.7
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                AC104581.22        52026-52087
            63-5323             U91543.1           1-5261
            5324-5926           AK125928.1         1678-2280
            5927-6833           U91543.1           5865-6771
            6834-7385           BC110648.1         2605-3156
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..7385
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /note="chromodomain helicase DNA binding protein 3"
                     /db_xref="GeneID:1107"
                     /db_xref="HGNC:HGNC:1918"
                     /db_xref="MIM:602120"
     exon            1..312
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..6215
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     zinc-finger helicase (Snf2-like); hZFH; CHD-3; zinc finger
                     helicase; ATP-dependent helicase CHD3; mi-2 autoantigen
                     240 kDa protein"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 3
                     isoform 1"
                     /protein_id="NP_001005273.1"
                     /db_xref="CCDS:CCDS32554.1"
                     /db_xref="GeneID:1107"
                     /db_xref="HGNC:HGNC:1918"
                     /db_xref="MIM:602120"
                     /translation="MKAADTVILWARSKNDQLRISFPPGLCWGDRMPDKDDIRLLPSA
                     LGVKKRKRGPKKQKENKPGKPRKRKKRDSEEEFGSERDEYREKSESGGSEYGTGPGRK
                     RRRKHREKKEKKTKRRKKGEGDGGQKQVEQKSSATLLLTWGLEDVEHVFSEEDYHTLT
                     NYKAFSQFMRPLIAKKNPKIPMSKMMTILGAKWREFSANNPFKGSAAAVAAAAAAAAA
                     AVAEQVSAAVSSATPIAPSGPPALPPPPAADIQPPPIRRAKTKEGKGPGHKRRSKSPR
                     VPDGRKKLRGKKMAPLKIKLGLLGGKRKKGGSYVFQSDEGPEPEAEESDLDSGSVHSA
                     SGRPDGPVRTKKLKRGRPGRKKKKVLGCPAVAGEEEVDGYETDHQDYCEVCQQGGEII
                     LCDTCPRAYHLVCLDPELDRAPEGKWSCPHCEKEGVQWEAKEEEEEYEEEGEEEGEKE
                     EEDDHMEYCRVCKDGGELLCCDACISSYHIHCLNPPLPDIPNGEWLCPRCTCPVLKGR
                     VQKILHWRWGEPPVAVPAPQQADGNPDVPPPRPLQGRSEREFFVKWVGLSYWHCSWAK
                     ELQLEIFHLVMYRNYQRKNDMDEPPPLDYGSGEDDGKSDKRKVKDPHYAEMEEKYYRF
                     GIKPEWMTVHRIINHSVDKKGNYHYLVKWRDLPYDQSTWEEDEMNIPEYEEHKQSYWR
                     HRELIMGEDPAQPRKYKKKKKELQGDGPPSSPTNDPTVKYETQPRFITATGGTLHMYQ
                     LEGLNWLRFSWAQGTDTILADEMGLGKTIQTIVFLYSLYKEGHTKGPFLVSAPLSTII
                     NWEREFQMWAPKFYVVTYTGDKDSRAIIRENEFSFEDNAIKGGKKAFKMKREAQVKFH
                     VLLTSYELITIDQAALGSIRWACLVVDEAHRLKNNQSKFFRVLNGYKIDHKLLLTGTP
                     LQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADV
                     FKNMPAKTELIVRVELSPMQKKYYKYILTRNFEALNSRGGGNQVSLLNIMMDLKKCCN
                     HPYLFPVAAMESPKLPSGAYEGGALIKSSGKLMLLQKMLRKLKEQGHRVLIFSQMTKM
                     LDLLEDFLDYEGYKYERIDGGITGALRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLA
                     TADTVIIFDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRASVEERITQVAKRKMML
                     THLVVRPGLGSKAGSMSKQELDDILKFGTEELFKDENEGENKEEDSSVIHYDNEAIAR
                     LLDRNQDATEDTDVQNMNEYLSSFKVAQYVVREEDKIEEIEREIIKQEENVDPDYWEK
                     LLRHHYEQQQEDLARNLGKGKRVRKQVNYNDAAQEDQDNQSEYSVGSEEEDEDFDERP
                     EGRRQSKRQLRNEKDKPLPPLLARVGGNIEVLGFNTRQRKAFLNAVMRWGMPPQDAFT
                     TQWLVRDLRGKTEKEFKAYVSLFMRHLCEPGADGSETFADGVPREGLSRQQVLTRIGV
                     MSLVKKKVQEFEHINGRWSMPELMPDPSADSKRSSRASSPTKTSPTTPEASATNSPCT
                     SKPATPAPSEKGEGIRTPLEKEEAENQEEKPEKNSRIGEKMETEADAPSPAPSLGERL
                     EPRKIPLEDEVPGVPGEMEPEPGYRGDREKSATESTPGERGEEKPLDGQEHRERPEGE
                     TGDLGKREDVKGDRELRPGPRDEPRSNGRREEKTEKPRFMFNIADGGFTELHTLWQNE
                     ERAAISSGKLNEIWHRRHDYWLLAGIVLHGYARWQDIQNDAQFAIINEPFKTEANKGN
                     FLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNLSQEPAHPAMALHARFAEAECLAES
                     HQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPATLSRIPPIAARLQMSE
                     RSILSRLASKGTEPHPTPAYPPGPYATPPGYGAAFSAAPVGALAAAGANYSQMPAGSF
                     ITAATNGPPVLVKKEKEMVGALVSDGLDRKEPRAGEVICIDD"
     exon            313..425
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            426..596
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            597..721
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            722..1005
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1136
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1287
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1481
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1715
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1919
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2131
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2263
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2363
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2555
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2756
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            2757..2894
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            2895..3016
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3190
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3332
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3464
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3582
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3583..3707
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3708..3939
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            3940..4106
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4107..4284
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4350
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4351..4436
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4570
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4571..4715
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4716..4878
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            4879..5000
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5001..5136
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5137..5238
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5239..5364
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5365..5473
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5474..5606
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5607..5802
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5803..5966
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            5967..6093
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     exon            6094..7385
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6806..6811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /note="hexamer: AATAAA"
     polyA_site      6834
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /note="major polyA site"
     regulatory      7365..7370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /note="hexamer: TATAAA"
     polyA_site      7385
                     /gene="CHD3"
                     /gene_synonym="Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH"
                     /note="major polyA site"
ORIGIN      
        1 atatctttgt ttgggtctcc catactgcgt atagatgaat gggtcaggat atctggaaca
       61 aaatatggag gtgaagggtg agatcgggaa acaaagggta tggcccccta gttcccaaag
      121 ggagcaggga gatgggaata gaattgaagg taggttttag gctacttggg aggaggaata
      181 tttaggtaat tgtggagact ttctcctgtg tgatgaaggc ggcagacact gtgatcctgt
      241 gggcaagaag taaaaatgac cagctgagga tttcttttcc tccaggactg tgttggggtg
      301 acaggatgcc tgataaggat gacattcggc tgctgccgtc agcattgggt gtgaagaaga
      361 gaaaacgagg acccaagaag cagaaggaga acaagccagg aaaaccccga aaacgcaaga
      421 agcgtgacag tgaggaggaa tttggttctg agcgagatga gtaccgggag aagtcagaga
      481 gtgggggcag tgaatatgga accggaccgg gtcggaaacg aagaaggaag caccgagaaa
      541 aaaaggagaa gaagacaaag cggcggaaaa agggggaggg agatgggggg caaaagcaag
      601 tggaacagaa gtcatcagca actctgcttc tgacctgggg cctggaggat gtggagcatg
      661 tgttctctga ggaggattac cacacgctca ccaactacaa agccttcagc cagttcatga
      721 ggcccctaat tgctaagaag aatcctaaga tcccaatgtc taagatgatg accatccttg
      781 gggccaaatg gagagagttc agtgccaaca accccttcaa ggggtcagca gctgctgtgg
      841 cggcggcagc ggcagcagca gcagcagctg tagctgagca ggtgtcagct gctgtctcgt
      901 cggccacccc catagcaccc tccggacccc ccgcccttcc accaccccct gctgctgata
      961 tccagccccc acccatccga agagccaaaa ccaaagaggg caaaggtcca ggccataaga
     1021 ggcggagtaa gagcccccga gtgcctgatg gacgcaagaa gcttcgggga aagaaaatgg
     1081 caccactcaa aataaaacta gggcttctgg gtggcaagag gaagaaagga ggctcgtatg
     1141 tttttcagag cgacgaaggt cctgaaccag aggctgagga atcagacctg gacagtggca
     1201 gtgtccacag tgcctcaggc cggcctgatg gccctgtccg caccaagaaa ctaaagagag
     1261 gccggccagg aaggaagaag aagaaggtcc tgggctgtcc tgcagtggcc ggggaggagg
     1321 aggttgatgg ctacgagacg gatcaccagg attactgtga ggtgtgccag cagggtgggg
     1381 aaattattct gtgtgacacc tgccctcgtg cctaccacct cgtctgcctt gatcctgagc
     1441 ttgaccgggc tccagagggc aaatggagct gccctcactg tgagaaggag ggggtccagt
     1501 gggaggccaa ggaggaagaa gaagaatacg aagaggaggg agaggaagaa ggggagaagg
     1561 aggaggagga tgatcacatg gagtactgcc gcgtatgcaa ggacggcggg gagctcctgt
     1621 gctgtgacgc gtgcatctcc tcctaccaca ttcattgtct aaaccctccc ctgcctgaca
     1681 ttcccaatgg tgaatggctg tgtccccgat gcacatgccc cgtgctgaag ggtcgagtgc
     1741 agaagatcct acattggcgg tggggggagc cacctgtagc agtgccagcc cctcaacagg
     1801 cagatggaaa tccagatgtc ccaccccccc gtcctcttca aggcagatca gagcgagagt
     1861 tctttgtcaa gtgggtagga ctatcctact ggcactgctc ctgggccaag gagcttcagc
     1921 tggaaatctt ccatttggtt atgtatcgaa actaccagcg gaagaatgac atggatgagc
     1981 ccccacccct ggactatggc tccggcgagg atgatgggaa gagcgacaag cgtaaagtga
     2041 aagacccgca ctatgctgag atggaggaga agtactatcg ttttggcatc aagccagagt
     2101 ggatgaccgt ccaccgcatc atcaaccaca gtgtggataa aaaggggaat taccactatc
     2161 tagtaaaatg gagggactta ccatatgacc agtccacgtg ggaggaagat gaaatgaata
     2221 tccctgaata cgaagaacat aagcaaagct actggagaca ccgagaacta attatggggg
     2281 aagaccctgc ccagccccgc aagtataaga agaagaagaa ggagctacag ggtgatgggc
     2341 ctcccagttc tcccactaat gatcctaccg tgaaatatga gactcagcca cggtttatca
     2401 cagccactgg aggcaccctg cacatgtatc agttggaagg gctgaactgg ctacgcttct
     2461 cctgggccca gggcactgac accattctag ctgatgagat ggggctaggc aagaccatac
     2521 aaaccatcgt cttcctctac tcactctaca aggagggcca cacaaaaggt cccttcctgg
     2581 tgagtgcccc actctctacc atcattaact gggagcggga gttccagatg tgggcaccca
     2641 aattctatgt ggtgacatac acgggtgaca aggacagccg ggccatcatt cgtgagaatg
     2701 aattctcctt tgaggacaat gccatcaaag ggggcaagaa agcttttaag atgaagaggg
     2761 aggcacaggt gaagttccat gttctcctga catcgtatga gctgatcacc attgatcagg
     2821 cagcacttgg ttccatccgc tgggcctgtc ttgtggtaga tgaggcccat cgactcaaga
     2881 acaaccagtc caagtttttc agggttctca atggttacaa gatagatcat aagttgctgc
     2941 tgacaggaac cccattgcag aataatctgg aggagctctt ccatctcctg aacttcctca
     3001 ccccagagag atttaacaac ttggagggct tcctggagga gtttgctgac atatccaaag
     3061 aggaccagat caagaaactg catgatttgc tggggccaca catgctgcgg agactcaagg
     3121 cagatgtctt taagaacatg ccagccaaga cagagctcat cgttcgggtg gagctaagcc
     3181 ccatgcagaa gaaatactac aaatacatcc tgactcgaaa ttttgaggcc ttgaattcac
     3241 gaggtggtgg gaaccaggtg tcgctgctta atatcatgat ggatcttaag aagtgctgca
     3301 accatccata cctttttccc gtggctgcta tggagtcccc caaactcccc agtggggctt
     3361 atgagggtgg ggcacttatt aagtcgtctg ggaagctcat gctgctccag aagatgctgc
     3421 gaaagctgaa ggagcaagga caccgagtgc tcatcttctc gcagatgacc aaaatgttag
     3481 acttgcttga ggacttctta gactatgaag gctacaagta tgagcgcatc gatggtggta
     3541 tcacgggtgc cctgaggcag gaggccatcg atcggtttaa tgctcctggg gcccaacaat
     3601 tctgcttcct cctgtccacc cgagctgggg gcctgggcat caatctggcc actgctgaca
     3661 ctgtcatcat ctttgattct gactggaacc cccataatga catccaggcc tttagccggg
     3721 ctcatcggat tggccaggcc aacaaagtga tgatttaccg gtttgtgact cgcgcgtcag
     3781 tggaagagcg aatcacacaa gtggccaaga gaaagatgat gctgacacac ctggttgtgc
     3841 ggcctgggct gggctccaag gcaggctcca tgtccaagca ggagcttgac gacattctca
     3901 aatttggcac tgaagagcta ttcaaggatg aaaacgaggg ggagaacaag gaggaggaca
     3961 gcagtgtgat tcattatgac aatgaggcca tcgctcggct gttggaccgg aaccaggatg
     4021 caactgagga cactgacgtg cagaacatga atgagtatct cagctccttc aaggtggcac
     4081 agtacgtcgt gcgggaagaa gacaagattg aggaaattga gcgagagatc atcaagcagg
     4141 aggagaatgt ggaccctgac tactgggaga agctgctgag gcatcactat gagcaacagc
     4201 aggaagacct agcccggaat ctaggcaagg gcaagcgggt tcgcaagcaa gttaactaca
     4261 atgatgctgc tcaggaagac caagacaacc agtcagagta ctcggtgggt tcagaggagg
     4321 aggatgaaga cttcgatgaa cgtcctgaag ggcgtagaca gtcaaagagg cagctccgga
     4381 atgagaaaga taagccactg cctccactgc tggcccgagt cgggggcaac attgaggtgc
     4441 tgggcttcaa cacccgtcag cggaaggctt tcctcaatgc tgtgatgcgc tgggggatgc
     4501 caccacagga tgccttcacc acacagtggc tggtgcggga cctgaggggc aagactgaga
     4561 aggagtttaa ggcctatgtg tctttgttca tgcgccatct gtgtgagcct ggggcagacg
     4621 gctctgaaac ctttgccgat ggggtccctc gggagggact gagtcgccag caggtgttga
     4681 cccgcattgg agtcatgtct ctcgtcaaaa agaaggtgca ggagtttgag cacatcaatg
     4741 ggcgttggtc aatgccggaa ctgatgcctg accccagcgc cgattctaag cgctcctcca
     4801 gagcctcctc tcctaccaaa acgtctccca ccactcctga ggcttctgct accaacagtc
     4861 cctgcacctc taaacctgct actccagctc caagtgagaa aggagaaggc ataaggacac
     4921 ctcttgagaa ggaggaagct gaaaaccagg aggaaaagcc agagaagaac agcagaattg
     4981 gggagaagat ggagacagag gctgatgccc ccagcccagc cccatcactt ggggagcggc
     5041 tggagccaag gaagattcct ctagaggatg aggtgccagg ggtgcctgga gagatggagc
     5101 ctgaacctgg gtaccgtggg gacagagaga agtcagccac agagtcgacg ccaggagaaa
     5161 ggggggagga gaagccgttg gatggacagg aacacaggga gaggccggag ggggaaacag
     5221 gggatttggg caagagagaa gatgtaaaag gtgaccggga gcttcgacca gggcctcgag
     5281 atgagccacg gtccaatggg cgacgagagg aaaagacaga gaagccccgg ttcatgttca
     5341 atatcgccga tggtggcttc acagagcttc acacactgtg gcagaatgag gaacgggcag
     5401 ctatttcctc ggggaaactc aatgagatct ggcacagaag acatgactat tggcttctgg
     5461 ctgggattgt cctccatggc tatgcacggt ggcaggacat ccagaatgat gctcaatttg
     5521 ccattatcaa cgagccattt aaaactgaag ccaataaggg gaactttctg gagatgaaaa
     5581 ataagttcct ggcccggagg ttcaagctcc tggagcaggc gctggtgatt gaggagcagc
     5641 tgcggcgggc ggcctacctg aacctgtcgc aggagccggc gcaccccgcc atggccctcc
     5701 acgcccgctt cgccgaggcc gagtgcctgg ccgagagcca ccagcacctc tccaaggagt
     5761 cgctggcggg gaacaagccg gccaacgccg tcctgcacaa ggttctgaac cagctggagg
     5821 agttgctgag cgacatgaag gcggacgtga cccgcctgcc agccacgctg tcccgaatac
     5881 cccccatcgc agcccgcctt cagatgtccg agcgcagcat cctcagccgg ctggccagca
     5941 agggcacgga gcctcacccc acaccggcct acccgccggg tccctacgct acacctccgg
     6001 ggtacggggc ggccttcagc gccgcacccg taggggccct ggccgccgca ggcgccaatt
     6061 acagccagat gcctgcaggg tccttcatca cagccgccac caacggccct ccagtgcttg
     6121 tgaagaagga gaaggaaatg gtgggggcat tggtgtcaga cgggctggat cggaaggagc
     6181 cccgagccgg ggaggtgatc tgtatagacg actgactgga tcccaggcct gcccttcacc
     6241 caggccccgt ccccgaggcc gacccccagc tcaagcgctg gggcctgctg ccagccctcc
     6301 accttcccca ccccttgggc catcactggg ctaggaaccc ctttgcccct ctctgcagct
     6361 cctctcttca agaagggccc tttgtctttc tccactccca cacacctttc ccaccaagcc
     6421 ttgaagactg tgctggtgag aagaagtctg ggtgggagat ggctggcagg gtcttccaag
     6481 taccttcctc ccacactgcc aagtatacac aacttcccag taaatggttg tggggaggaa
     6541 agaggtggag cctccccagc cgtttccctg cagaatcagc tctgtctcat gtggaagtgg
     6601 agaatcagcc ttgcctggcc tttaggaact tttgtgggga agagagcttt gaagagagga
     6661 gggggacttt agagagggat gaaaatgagc cctgggaggg aggaagggac gaggaggggt
     6721 ggctgcatgt taccgtcccc tacctctccc cacgtggagg gtggagcagt tatgagggag
     6781 gaagtcaact gctgttcagc ctcagaataa aggtgccgtt cactggctca gttacctcct
     6841 gtgtaccggc atcttgtgtt gggaatgttc ccccctccct agggaccaag gaccacccct
     6901 acaaaaagag taatggttgg gtgatactcc ctcaagccaa agaggagctc cccaacctgt
     6961 tctagggacc caggtaacct agaagggtgg gagagaatac aatgggccag atgtggtgga
     7021 agcccagctc tggggctcag gttcctggaa gacttctact accctccctc ctcaaggcct
     7081 ggatacagac taaatttgta taagtcaggc aggggaccta gtcagggtct tgggagctac
     7141 cttgtcgttg ggaccagagc aaaatagtgg agggcaggct agggaaatgt gggcacatcc
     7201 cccctcccag gaggggccgg ggagagtggc agtttgcatg gcgaaccccc cacttcctct
     7261 ttgctgcccc ttcactttct tgctgcccct ttcccagtct ctcttcacac ccactcctgg
     7321 tctgtcctga tcccctcttc tgtatcaggt ttattggttg tacatataaa ttatactttc
     7381 ctttc
//
