LOCUS       NM_001271               9350 bp    mRNA    linear   PRI 28-APR-2025
DEFINITION  Homo sapiens chromodomain helicase DNA binding protein 2 (CHD2),
            transcript variant 1, mRNA.
ACCESSION   NM_001271
VERSION     NM_001271.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   You,C., Xu,L., Zhu,L., Qiu,S., Xu,N., Wang,Y. and Yang,L.
  TITLE     Clinical analysis of five CHD2 gene mutations in Chinese children
            with epilepsy
  JOURNAL   Seizure 121, 38-44 (2024)
   PUBMED   39068850
  REMARK    GeneRIF: Clinical analysis of five CHD2 gene mutations in Chinese
            children with epilepsy.
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Cogliati,F., Straniero,L., Rimoldi,V., Masciadri,M., Perego,S.,
            Rinaldi,B., Milani,D., Gentilini,D., Larizza,L., Asselta,R.,
            Russo,S. and Bedeschi,M.F.
  TITLE     Low-grade parental gonosomal mosaicism in CHD2 siblings with
            Smith-Magenis-like syndrome
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 195 (6), e32976 (2024)
   PUBMED   38385826
  REMARK    GeneRIF: Low-grade parental gonosomal mosaicism in CHD2 siblings
            with Smith-Magenis-like syndrome.
REFERENCE   3  (bases 1 to 9350)
  AUTHORS   LaFlamme,C.W., Rastin,C., Sengupta,S., Pennington,H.E.,
            Russ-Hall,S.J., Schneider,A.L., Bonkowski,E.S., Almanza
            Fuerte,E.P., Allan,T.J., Zalusky,M.P., Goffena,J., Gibson,S.B.,
            Nyaga,D.M., Lieffering,N., Hebbar,M., Walker,E.V., Darnell,D.,
            Olsen,S.R., Kolekar,P., Djekidel,M.N., Rosikiewicz,W., McConkey,H.,
            Kerkhof,J., Levy,M.A., Relator,R., Lev,D., Lerman-Sagie,T.,
            Park,K.L., Alders,M., Cappuccio,G., Chatron,N., Demain,L.,
            Genevieve,D., Lesca,G., Roscioli,T., Sanlaville,D., Tedder,M.L.,
            Gupta,S., Jones,E.A., Weisz-Hubshman,M., Ketkar,S., Dai,H.,
            Worley,K.C., Rosenfeld,J.A., Chao,H.T., Neale,G., Carvill,G.L.,
            Wang,Z., Berkovic,S.F., Sadleir,L.G., Miller,D.E., Scheffer,I.E.,
            Sadikovic,B. and Mefford,H.C.
  CONSRTM   Undiagnosed Diseases Network; University of Washington Center for
            Rare Disease Research
  TITLE     Diagnostic utility of DNA methylation analysis in genetically
            unsolved pediatric epilepsies and CHD2 episignature refinement
  JOURNAL   Nat Commun 15 (1), 6524 (2024)
   PUBMED   39107278
  REMARK    GeneRIF: Diagnostic utility of DNA methylation analysis in
            genetically unsolved pediatric epilepsies and CHD2 episignature
            refinement.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9350)
  AUTHORS   Bao,Y., Wu,L., Liu,Y., Fan,C., Zhang,J. and Yang,J.
  TITLE     Role of CircCHD2 in the pathogenesis of gestational diabetes
            mellitus by regulating autophagy via miR-33b-3p/ULK1 axis
  JOURNAL   Placenta 145, 27-37 (2024)
   PUBMED   38039841
  REMARK    GeneRIF: Role of CircCHD2 in the pathogenesis of gestational
            diabetes mellitus by regulating autophagy via miR-33b-3p/ULK1 axis.
REFERENCE   5  (bases 1 to 9350)
  AUTHORS   Hu,P., Guo,J., Zhao,B., Zhang,Z., Zhu,J. and Liu,F.
  TITLE     CircCHD2/miR-200b-3p/HLF Axis Promotes Liver Cirrhosis
  JOURNAL   J Environ Pathol Toxicol Oncol 41 (4), 1-10 (2022)
   PUBMED   36374958
  REMARK    GeneRIF: CircCHD2/miR-200b-3p/HLF Axis Promotes Liver Cirrhosis.
REFERENCE   6  (bases 1 to 9350)
  AUTHORS   Zody,M.C., Garber,M., Sharpe,T., Young,S.K., Rowen,L., O'Neill,K.,
            Whittaker,C.A., Kamal,M., Chang,J.L., Cuomo,C.A., Dewar,K.,
            FitzGerald,M.G., Kodira,C.D., Madan,A., Qin,S., Yang,X., Abbasi,N.,
            Abouelleil,A., Arachchi,H.M., Baradarani,L., Birditt,B., Bloom,S.,
            Bloom,T., Borowsky,M.L., Burke,J., Butler,J., Cook,A.,
            DeArellano,K., DeCaprio,D., Dorris,L. 3rd, Dors,M., Eichler,E.E.,
            Engels,R., Fahey,J., Fleetwood,P., Friedman,C., Gearin,G.,
            Hall,J.L., Hensley,G., Johnson,E., Jones,C., Kamat,A., Kaur,A.,
            Locke,D.P., Madan,A., Munson,G., Jaffe,D.B., Lui,A., Macdonald,P.,
            Mauceli,E., Naylor,J.W., Nesbitt,R., Nicol,R., O'Leary,S.B.,
            Ratcliffe,A., Rounsley,S., She,X., Sneddon,K.M., Stewart,S.,
            Sougnez,C., Stone,S.M., Topham,K., Vincent,D., Wang,S.,
            Zimmer,A.R., Birren,B.W., Hood,L., Lander,E.S. and Nusbaum,C.
  TITLE     Analysis of the DNA sequence and duplication history of human
            chromosome 15
  JOURNAL   Nature 440 (7084), 671-675 (2006)
   PUBMED   16572171
REFERENCE   7  (bases 1 to 9350)
  AUTHORS   Hiller,M., Huse,K., Platzer,M. and Backofen,R.
  TITLE     Non-EST based prediction of exon skipping and intron retention
            events using Pfam information
  JOURNAL   Nucleic Acids Res 33 (17), 5611-5621 (2005)
   PUBMED   16204458
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 9350)
  AUTHORS   Johnson,J.M., Castle,J., Garrett-Engele,P., Kan,Z., Loerch,P.M.,
            Armour,C.D., Santos,R., Schadt,E.E., Stoughton,R. and
            Shoemaker,D.D.
  TITLE     Genome-wide survey of human alternative pre-mRNA splicing with exon
            junction microarrays
  JOURNAL   Science 302 (5653), 2141-2144 (2003)
   PUBMED   14684825
REFERENCE   9  (bases 1 to 9350)
  AUTHORS   Woodage,T., Basrai,M.A., Baxevanis,A.D., Hieter,P. and Collins,F.S.
  TITLE     Characterization of the CHD family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (21), 11472-11477 (1997)
   PUBMED   9326634
REFERENCE   10 (bases 1 to 9350)
  AUTHORS   Carvill,G.L. and Mefford,H.C.
  TITLE     CHD2-Related Neurodevelopmental Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   26677509
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF006514.1, DB121252.1,
            AC013394.12, DB111282.1, CT003638.1 and AA907775.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001271.3.
            
            Summary: The CHD family of proteins is characterized by the
            presence of chromo (chromatin organization modifier) domains and
            SNF2-related helicase/ATPase domains. CHD genes alter gene
            expression possibly by modification of chromatin structure thus
            altering access of the transcriptional apparatus to its chromosomal
            DNA template. Alternatively spliced transcript variants encoding
            distinct isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394196.9/ ENSP00000377747.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2620              AF006514.1         136-2755
            2621-2895           DB121252.1         291-565
            2896-3289           AF006514.1         3031-3424
            3290-3299           AC013394.12        63130-63139         c
            3300-4037           AF006514.1         3435-4172
            4038-4596           DB111282.1         1-559
            4597-5478           AF006514.1         4732-5613
            5479-5960           CT003638.1         224-705
            5961-8992           AC013394.12        13875-16906         c
            8993-9350           AA907775.1         22-379              c
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..9350
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /note="chromodomain helicase DNA binding protein 2"
                     /db_xref="GeneID:1106"
                     /db_xref="HGNC:HGNC:1917"
                     /db_xref="MIM:602119"
     exon            1..501
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            502..634
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     CDS             573..6059
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CHD-2; ATP-dependent helicase CHD2"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 2
                     isoform 1"
                     /protein_id="NP_001262.3"
                     /db_xref="CCDS:CCDS10374.2"
                     /db_xref="GeneID:1106"
                     /db_xref="HGNC:HGNC:1917"
                     /db_xref="MIM:602119"
                     /translation="MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSD
                     PGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEE
                     YPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQ
                     EQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEAP
                     KRQTRRRAAKNVSYKEDDDFETDSDDLIEMTGEGVDEQQDNSETIEKVLDSRLGKKGA
                     TGASTTVYAIEANGDPSGDFDTEKDEGEIQYLIKWKGWSYIHSTWESEESLQQQKVKG
                     LKKLENFKKKEDEIKQWLGKVSPEDVEYFNCQQELASELNKQYQIVERVIAVKTSKST
                     LGQTDFPAHSRKPAPSNEPEYLCKWMGLPYSECSWEDEALIGKKFQNCIDSFHSRNNS
                     KTIPTRECKALKQRPRFVALKKQPAYLGGENLELRDYQLEGLNWLAHSWCKNNSVILA
                     DEMGLGKTIQTISFLSYLFHQHQLYGPFLIVVPLSTLTSWQREFEIWAPEINVVVYIG
                     DLMSRNTIREYEWIHSQTKRLKFNALITTYEILLKDKTVLGSINWAFLGVDEAHRLKN
                     DDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFEFWEDFEEDHGKGR
                     ENGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVEMSALQKQYYKWILTRNYKAL
                     AKGTRGSTSGFLNIVMELKKCCNHCYLIKPPEENERENGQEILLSLIRSSGKLILLDK
                     LLTRLRERGNRVLIFSQMVRMLDILAEYLTIKHYPFQRLDGSIKGEIRKQALDHFNAD
                     GSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYR
                     LVTKGTVEEEIIERAKKKMVLDHLVIQRMDTTGRTILENNSGRSNSNPFNKEELTAIL
                     KFGAEDLFKELEGEESEPQEMDIDEILRLAETRENEVSTSATDELLSQFKVANFATME
                     DEEELEERPHKDWDEIIPEEQRKKVEEEERQKELEEIYMLPRIRSSTKKAQTNDSDSD
                     TESKRQAQRSSASESETEDSDDDKKPKRRGRPRSVRKDLVEGFTDAEIRRFIKAYKKF
                     GLPLERLECIARDAELVDKSVADLKRLGELIHNSCVSAMQEYEEQLKENASEGKGPGK
                     RRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCLTCRVKAAHFDVEWG
                     VEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQGKQLQTRADYLLKL
                     LRKGLEKKGAVTGGEEAKLKKRKPRVKKENKVPRLKEEHGIELSSPRHSDNPSEEGEV
                     KDDGLEKSPMKKKQKKKENKENKEKQMSSRKDKEGDKERKKSKDKKEKPKSGDAKSSS
                     KSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALKQLDKPDKGLN
                     VQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEFDARKLHKLY
                     KMAHKKRSQEEEEQKKKDDVTGGKKPFRPEASGSSRDSLISQSHTSHNLHPQKPHLPA
                     SHGPQMHGHPRDNYNHPNKRHFSNADRGDWQRERKFNYGGGNNNPPWGSDRHHQYEQH
                     WYKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHHHDSKRR
                     RSDEFRPQNYHQQDFRRMSDHRPAMGYHGQGPSDHYRSFHTDKLGEYKQPLPPLHPAV
                     SDPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPDYNWNVRKT"
     exon            635..866
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            867..953
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1015
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1123
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1264
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1398
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1624
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1725
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1770
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1949
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2074
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2291
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2381
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2572
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2761
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2924
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            2925..3077
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3078..3149
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..3299
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3448
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3545
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3546..3638
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3639..3809
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3810..3985
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            3986..4027
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4028..4167
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4168..4306
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4307..4457
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4458..4580
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4581..4709
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4710..4850
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4851..4985
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            4986..5164
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            5165..5264
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            5265..5478
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            5479..5725
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     exon            5726..9350
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9273..9278
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /note="hexamer: ATTAAA"
     polyA_site      9296
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /note="major polyA site"
     regulatory      9321..9326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /note="hexamer: ATTAAA"
     polyA_site      9350
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /note="major polyA site"
ORIGIN      
        1 agagctggga aggaggctct agatggcggc tgtgccttag agagagcgcg ctctgctccc
       61 tgcctttgcc tcactttacg caactttccc taactttcgg gcagcctcag ggggcccccg
      121 tagccccctg cctttcctag ggacttactg gggtcgattc gaaccttttt ttgggagaaa
      181 agcagctttt aggagctttc ttttcgtgcc ttgttggaaa gaagcagccg tactgagagc
      241 ccaggtcgtt gttttttcca gcttagaagc catggcgcac ctccattttt gtgcgctctc
      301 ctaatgaggt tttttttctt tcggacctgt tttagtatta attattgctt tatttttttg
      361 accagttaac atatttgagg gttattttat ttatttttcg ttttttaacg gaggattttg
      421 cctttatttt taattatttg ggatctgata tttttctact agtagatagg actcttggtt
      481 tggacatact acatggatca gtaaatacct gggcacagga cttcaaagca aacacagatt
      541 ccccctcccc cttaatattt aagaattaaa agatgatgag aaataaggac aaaagccaag
      601 aggaggacag ttcgctacac agcaatgcat cgagtcactc agcctctgaa gaagcttcgg
      661 gttcagactc aggcagtcag tcggaaagtg agcagggaag tgatccagga agtggacatg
      721 gcagcgagtc gaacagcagc tctgaatctt ctgagagtca gtcggaatct gagagcgaat
      781 cagcaggttc caaatcccag ccagtcctcc cagaagccaa agagaagcca gcctctaaga
      841 aggaacggat agctgatgtg aagaagatgt gggaagaata tcctgatgtt tatggggtca
      901 ggcggtcaaa ccgaagcaga caagaaccat cgcgatttaa tattaaggaa gaggcaagta
      961 gcgggtctga gagtgggagc ccaaaaagaa gaggccagag gcagctgaaa aaacaagaaa
     1021 aatggaaaca ggaaccctca gaagatgaac aggaacaagg caccagtgca gagagtgagc
     1081 cagaacaaaa aaaagtaaaa gccagaagac ctgtccccag aagaacagtg cccaaacctc
     1141 gtgttaaaaa gcagccgaag actcagcgtg gaaagagaaa aaagcaagat tcttctgatg
     1201 aggatgatga tgatgacgaa gctcccaaaa ggcagactcg tcgaagagcg gctaaaaacg
     1261 ttagttacaa agaagatgat gactttgaga ctgactcaga tgatctcatt gaaatgactg
     1321 gagaaggagt tgatgaacag caagataata gtgaaactat tgaaaaggtc ttagattcaa
     1381 gactgggaaa gaaaggagcc actggagcat ctactactgt atatgcgatt gaagctaatg
     1441 gcgaccctag tggtgacttt gacactgaaa aggatgaagg tgaaatccag tacctcatca
     1501 agtggaaggg ttggtcttac atccacagca catgggagag tgaagaatcc ttacagcaac
     1561 agaaagtgaa gggcctaaaa aaactagaga acttcaagaa aaaagaggac gaaatcaaac
     1621 aatggttagg gaaagtttct cctgaagatg tagaatattt caattgccaa caggagctgg
     1681 cttcagagtt gaataaacag tatcagatag tagaaagagt aatagctgtg aagacaagta
     1741 aatctacatt gggtcaaaca gattttccag ctcatagtcg gaagccggca ccctcaaatg
     1801 agcccgaata tctatgtaaa tggatgggac tcccctattc agagtgtagc tgggaagatg
     1861 aagccctcat tggaaagaaa ttccagaatt gcattgacag cttccacagt aggaacaact
     1921 caaaaaccat cccaacaaga gaatgcaagg ccctgaagca gagaccacga tttgtagctt
     1981 taaagaaaca acctgcatat ttaggagggg agaatctgga acttcgagat tatcagctag
     2041 aaggtctaaa ctggctagct cattcctggt gcaaaaataa tagtgtaatc cttgctgatg
     2101 aaatgggcct aggaaagacc atccagacca tatcattcct ctcctacctg ttccaccaac
     2161 accagctgta tggccccttt cttatagtcg tccctttatc caccctcacc tcatggcaga
     2221 gagagtttga aatctgggca ccagagatta acgtagtggt ttacataggt gacctgatga
     2281 gcagaaatac gatacgggaa tatgaatgga ttcattccca aaccaaaaga ttgaagttca
     2341 acgcacttat aacaacatat gagatcctct tgaaagataa gactgtgctg ggcagtatta
     2401 actgggcctt tctgggagtg gatgaagccc atcggttgaa gaatgatgac tctttattgt
     2461 ataaaactct gattgatttc aagtccaacc ataggctcct gattacgggg acccctcttc
     2521 agaattccct caaagagctc tggtccttgc tgcactttat tatgccggag aagtttgaat
     2581 tttgggaaga ttttgaagaa gaccatggga aggggagaga aaatggctac cagagtcttc
     2641 ataaggtgct agagcctttc cttctccgga gagtcaaaaa agatgtggag aaatcccttc
     2701 ctgctaaagt ggaacagatt ctcagggtgg agatgtcagc ccttcagaaa cagtattaca
     2761 agtggattct gaccaggaat tacaaggctc ttgccaaagg aacaagaggc agcacatctg
     2821 gttttcttaa tattgtgatg gaactgaaaa aatgttgcaa ccactgctat ctgattaaac
     2881 cccctgaaga aaatgaaagg gaaaatggac aggagattct tctgtccctc ataaggagca
     2941 gtgggaagtt gattttatta gacaaactgt tgacaagact tcgagaaagg gggaatcgag
     3001 tgcttatctt ctctcagatg gtgagaatgt tggatatcct ggctgaatac ctaactatta
     3061 aacactatcc tttccagcgt ctggatggtt ccatcaaggg agaaatccga aaacaggcac
     3121 tggaccactt caatgcagat gggtctgagg acttctgttt cctgctctcg acaagggctg
     3181 gtggcctggg aatcaatttg gcttcagcgg acacagtcgt catctttgac tctgactgga
     3241 acccccagaa tgacttgcag gcacaagccc gagcgcatag aattggtcaa aagaagcagg
     3301 taaatattta ccgcttagtt acaaagggga ctgtggagga ggagatcata gaacgggcca
     3361 aaaagaagat ggtattagat catctggtga ttcagcgcat ggacaccact ggccggacga
     3421 tcctggaaaa caactcagga aggtccaact caaatccttt taataaagaa gagctgacag
     3481 ctattttgaa atttggagca gaggatctct tcaaagaact ggaaggggag gaatcagaac
     3541 ctcaggaaat ggatatagat gaaattttgc ggttggctga aacgagagag aatgaagtgt
     3601 caacaagtgc aacagatgaa cttctatcac agtttaaggt tgccaacttt gcaacaatgg
     3661 aagatgaaga agagctagaa gagcgtcctc acaaggactg ggatgagatc attccagagg
     3721 aacaaaggaa aaaagtagag gaggaagagc ggcagaagga gctagaagaa atttatatgc
     3781 tgcctcgaat tcggagttcc actaaaaagg ctcagacaaa tgacagtgac tctgacactg
     3841 agtctaagag gcaggcccag agatcctctg cttctgagag tgaaacggaa gactctgatg
     3901 atgacaagaa gccaaagcgc agagggcgtc cgaggagtgt gcggaaggac ctcgtggagg
     3961 gatttactga tgcagagatc cgaaggttca tcaaggctta taagaagttt ggtctccctc
     4021 ttgaacggct ggagtgcata gcacgtgatg ctgagctggt agataagtcg gtggcagatc
     4081 tgaagcgcct gggtgaactg atccacaaca gctgtgtgtc agcaatgcag gaatacgaag
     4141 agcagctgaa agaaaatgcc agcgagggaa aaggaccagg gaaaaggaga ggtccaacaa
     4201 tcaagatatc cggagttcag gttaatgtga aatccattat ccaacatgaa gaggagtttg
     4261 agatgctgca taaatctatc cctgtggacc ctgaagaaaa aaaaaaatac tgcttaacct
     4321 gtcgtgtcaa agctgcacat tttgatgtag agtggggggt ggaagatgat tctcgcctgt
     4381 tgctggggat ttatgaacat ggctatggaa actgggagtt aattaaaaca gacccagagc
     4441 ttaaattaac tgacaaaatt ctgccggtgg agacagataa aaagcctcag gggaagcagc
     4501 tacagacccg agcggattac ttgttgaagc tgctcagaaa gggtctggag aagaaggggg
     4561 ctgtgacagg tggggaagag gccaaattaa agaagcggaa gcctcgggta aagaaggaaa
     4621 acaaagtgcc caggctgaaa gaggagcatg gaattgagct ttcatctcct aggcattcag
     4681 ataatccatc agaagaggga gaagtgaaag atgatggctt ggaaaaaagt ccaatgaaaa
     4741 aaaaacagaa gaagaaagag aacaaggaga acaaggagaa acaaatgagt tctaggaaag
     4801 acaaagaagg ggacaaggaa agaaagaagt caaaagataa gaaagagaag cctaaaagtg
     4861 gtgatgccaa atcttcgagt aaatcaaagc gatctcaggg tcctgtccat attacagcag
     4921 gaagtgaacc tgtccccatt ggagaggatg aggatgatga tctggaccag gagacattca
     4981 gcatatgtaa ggagaggatg aggcccgtga aaaaggcact gaaacagctc gacaaacctg
     5041 acaaggggct caacgtgcaa gaacagctgg aacacacccg gaactgcctg ctgaaaatcg
     5101 gagaccggat agccgagtgc cttaaagcct actcagatca ggagcacatc aaactctgga
     5161 ggaggaacct atggattttt gtttccaagt ttacagaatt tgatgctcga aaactgcata
     5221 agttatacaa gatggctcat aagaaaaggt ctcaagaaga agaggagcaa aagaagaaag
     5281 acgacgtgac tgggggtaag aaaccatttc gtccagaggc ctcaggctcc agccgggact
     5341 ctctgatatc tcagtcccat acctcacaca accttcaccc tcagaagcct catttgcctg
     5401 cctcccatgg cccacagatg catggacacc caagagataa ctacaatcac cccaacaaga
     5461 gacacttcag taatgcagat cgaggagact ggcagaggga aagaaagttc aactatggtg
     5521 gtggcaacaa caatccacca tggggaagcg acaggcacca tcagtatgag cagcactggt
     5581 acaaggacca ccattatggg gaccggcgac atatggatgc ccaccgttcc ggaagctatc
     5641 gacccaacaa catgtccaga aagaggcctt atgaccagta cagcagtgac cgagaccacc
     5701 ggggacacag agattattat gacaggcacc atcatgactc caagcggagg agatccgatg
     5761 aatttaggcc tcaaaattac caccagcagg atttccgacg aatgtctgat caccgccccg
     5821 ctatgggcta ccatggccag ggaccctcag accattaccg ctctttccac acagataaac
     5881 tgggggaata taaacagcct ctacccccat tgcaccctgc agtctcagat cctcgctcac
     5941 ccccttctca gaaatctcct cacgattcca agtcacccct ggatcatagg tctcctttgg
     6001 agagatcact agaacagaaa aacaacccag attataactg gaatgttcgg aaaacataaa
     6061 ggacagctcg taaaggagag agtaagagtc accaaacacg tggatatttt tggtctgatc
     6121 ctacagtagc cggttatcta gaccagtaag tggagttttg gacatgctgc tgctgtcaac
     6181 tcactggctg aaggagcact tcaaggaatg ggaggccttt cactgggtcc agctctgatt
     6241 cgggtcacca ctcctgcact ttggcacccc atcccattcc agcctagttc tggcctccca
     6301 ctttgacggg cacttggagg aggagctgac tgtgtgtgta ccagcttcac tgggatgtgt
     6361 ttccccagtc aaggaacagg ggatcttcag agtcatgaat gttttcttgc cagggtcagt
     6421 gttcccagga ccttagtgca tggtcggggc aggaactggt gcatggaggc tgctgggacc
     6481 tggtgaacag tgtgtgattt ggttgatttg gttcactctg acatgatgga tgctgctgat
     6541 ggggagtggc gagttggggc aagcgggtgg ggacaagcat aggacttgaa ggggagcagg
     6601 tacacccctc aaatgtgttc ttgggagaag tgatacactc ggcctcatta tgtgaaacct
     6661 gtgggtgggg ttggggtgga gaagtagaga gagggcaaca gcttccacaa ctgcttcatc
     6721 tctgccaaca ctaatttttc ccacactgtc tttgtacatt tcagagcttt ggtctcctga
     6781 gtgggcctcc ttttcccact gtgccaggga aggtaggttt ctctggctga ctgagtactg
     6841 tgagtgaggc aacactgatg ccagcatggg tcttacttga ctggggagta ggctgagtga
     6901 ggggtagggt ggggtaggtg gggagtagtg tggccaggga actggaatcc ctggtggatt
     6961 tctgattcct gtggtgagaa ggaaagctac aggacctgga gaaggggatg cagaggcagg
     7021 cctgctgacc aacttgttgc aatcacaaag gtgggggtcc tggtgcaggc agtgaacagg
     7081 cttctaatgt ggggttcagt agtgccagca agtgggggaa actttcagta ttgcgctagg
     7141 tcaacacttc ctgctgcatt tccttccctt tgcacagctt gaagaaatag agtagacaga
     7201 atcacacatc atgtggtggg cagatggaaa taagtacctg tggtgaacaa gtttctactg
     7261 tagttggaga tcattagaat tgaattcagt ttctcttaga atataatcag gtataaacct
     7321 aagttaaact ttttcccaaa caaggagcat ccaaagacac agtgacttga gctatagata
     7381 gtaaaaatca tacgagagtt gaactgagtc aggtttagga agcaagtttg gttgcatcaa
     7441 ttaagcaggc tcttttcaat tgactgatgc tggggccttc agttttattc tcagtataga
     7501 ttgccagtat tgttaagagt atccaaaggc ctttctagat ggagacagaa taactgactt
     7561 gaacatacag tgtgcctgta agtgtccagg ctcagagctg gtgaaaaccc ttctgttggg
     7621 cgtgtgcagg gttaaactcc tgaagtaact tgtgaggact tcagtgcttg ctggtgtcct
     7681 gggcagcacc atgaatgcct ttaccaagac atgccaagtt ggatcccccg aatgaagcag
     7741 atgtggctgt ggtgtgaccc ttgctccctg ctacacagag catcgcaggg ctggcctgtg
     7801 tggtttccag atgagggtct gggtcccgga agcttgtgtt gagagctcag tggacccacc
     7861 ccgcttgctg aaccctcaca gttcttgggg ttgtccagcc tggacttgta gcacacatgt
     7921 cctgaagcaa agctccggtg actgcatgag ccacctggcc acagtcctcc catggagggc
     7981 ctgccgtgac gctcagtgga gagggcaggg cctgtgtctc cacttaggcc acacagtgat
     8041 gaggaaacca cagatggagc ttcttgccga taatactgac tctagcccat gattgccttg
     8101 cccaagccaa agaggaaggt taggttggct tgtcgagccc ttgagcgttg ggagatgggg
     8161 tgggaaggag gtgagcccct gcagagagtt gggtagtgtc cttcaggaat gaaaggaggg
     8221 gcaaaggagt caccagaggt cctgcatttc catcagggtt tccacagtca tcagggcttc
     8281 tctcttgagt tgctgatagg agatgtgagt tatgcccaga gatgtcttat cgtgaggaaa
     8341 aagaaacttc cttttgttca catttaggac tctcagtgcc atatgaagta gcaaaaggca
     8401 gtatcggcca gatcagtgtt acattgattc taaaattaca gtgtccccat tagacaacta
     8461 ttttaggtgc tggagtatgt ttgaagagtg tgctgggaaa aaggaagcat ttcttcattg
     8521 atttaaatca gtatgaatat tatatgccta aataaaaaat ttgcacaggt aaattctctc
     8581 acttgtgaat gggagaagct gccccaggaa tctgtgagga tggtattccc tggagtctgg
     8641 ctttgaaaga tttcattgtt ggtagaaata acaggttgag aaagagggag ttagcatcac
     8701 ctaaaacctg cacgtgaaca agggttgaca tgatacacta tggccttaga aaagggccag
     8761 gtgaaacccc aagctaatca ctgcggtctt tcagagccgg acagacaggt gccagcagag
     8821 cccggggctc actctccttt cagtcattcc tcagcccttc gaagggaagc ccaaacactt
     8881 tgcacgctgt gctgcagaca ttctggcctg gtgtgtctga aagttgcatc agtcctcacg
     8941 gtgcaaacac agttgattta ggaagtcaca caatgacact gaaatcctac agaccaaaat
     9001 ccacttgtca gcaggagcag cagcccaggc ccagcaccag cggtcttccg gctcctctga
     9061 gggctgccac gttgggcgag gggagccatg ccaagggtcc aggctgcttt aggccatctg
     9121 tgccccactc atctggggac agatggtttt tctttattgt aaaattgtgg acttttaaaa
     9181 cctgttgact aaacagtaat taatttatat ttgtgaaaaa tgccactgtc ctagtgattt
     9241 ctgatgtaaa taatgttgtt tatatagtat gtattaaatt ttcctacatt gtaaaactgc
     9301 tgtacttttg attcttgtat attaaaaagt gttactgagc atttttagaa
//
