LOCUS       NM_005441               4466 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens chromatin assembly factor 1 subunit B (CHAF1B), mRNA.
ACCESSION   NM_005441
VERSION     NM_005441.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Saleiro,D., Kosciuczuk,E.M., Fischietti,M., Perez,R.E., Yang,G.S.,
            Eckerdt,F., Beauchamp,E.M., Hou,Y., Wang,Q., Weinberg,R.S.,
            Fish,E.N., Yue,F., Hoffman,R. and Platanias,L.C.
  TITLE     Targeting CHAF1B Enhances IFN Activity against Myeloproliferative
            Neoplasm Cells
  JOURNAL   Cancer Res Commun 3 (5), 943-951 (2023)
   PUBMED   37377894
  REMARK    GeneRIF: Targeting CHAF1B Enhances IFN Activity against
            Myeloproliferative Neoplasm Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Shrestha,R.L., Balachandra,V., Kim,J.H., Rossi,A., Vadlamani,P.,
            Sethi,S.C., Ozbun,L., Lin,S., Cheng,K.C., Chari,R., Karpova,T.S.,
            Pegoraro,G., Foltz,D.R., Caplen,N.J. and Basrai,M.A.
  TITLE     The histone H3/H4 chaperone CHAF1B prevents the mislocalization of
            CENP-A for chromosomal stability
  JOURNAL   J Cell Sci 136 (10) (2023)
   PUBMED   37129573
  REMARK    GeneRIF: The histone H3/H4 chaperone CHAF1B prevents the
            mislocalization of CENP-A for chromosomal stability.
REFERENCE   3  (bases 1 to 4466)
  AUTHORS   Geis,F.K., Sabo,Y., Chen,X., Li,Y., Lu,C. and Goff,S.P.
  TITLE     CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in
            infection
  JOURNAL   Proc Natl Acad Sci U S A 119 (4) (2022)
   PUBMED   35074917
  REMARK    GeneRIF: CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs
            early in infection.
REFERENCE   4  (bases 1 to 4466)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4466)
  AUTHORS   Duan,Y., Liu,T., Li,S., Huang,M., Li,X., Zhao,H. and Li,J.
  TITLE     CHAF1B promotes proliferation and reduces apoptosis in 95-D lung
            cancer cells and predicts a poor prognosis in non-small cell lung
            cancer
  JOURNAL   Oncol Rep 41 (4), 2518-2528 (2019)
   PUBMED   30720130
  REMARK    GeneRIF: Study revealed that CHAF1B was highly expressed in
            nonsmall cell lung cancer (NSCLC) lung tissues and 95D cells.
            Survival analysis indicated that high CHAF1B expression in tumor
            tissue was associated with poor clinical outcomes in NSCLC
            patients. CHAF1B knockdown in 95D cells markedly inhibited tumor
            proliferation, reduced colony formation, induced cell cycle arrest
            and promoted apoptosis.
REFERENCE   6  (bases 1 to 4466)
  AUTHORS   Verreault,A., Kaufman,P.D., Kobayashi,R. and Stillman,B.
  TITLE     Nucleosome assembly by a complex of CAF-1 and acetylated histones
            H3/H4
  JOURNAL   Cell 87 (1), 95-104 (1996)
   PUBMED   8858152
REFERENCE   7  (bases 1 to 4466)
  AUTHORS   Katsanis,N. and Fisher,E.M.
  TITLE     The gene encoding the p60 subunit of chromatin assembly factor I
            (CAF1P60) maps to human chromosome 21q22.2, a region associated
            with some of the major features of Down syndrome
  JOURNAL   Hum Genet 98 (4), 497-499 (1996)
   PUBMED   8792829
REFERENCE   8  (bases 1 to 4466)
  AUTHORS   Matsumoto-Taniura,N., Pirollet,F., Monroe,R., Gerace,L. and
            Westendorf,J.M.
  TITLE     Identification of novel M phase phosphoproteins by expression
            cloning
  JOURNAL   Mol Biol Cell 7 (9), 1455-1469 (1996)
   PUBMED   8885239
REFERENCE   9  (bases 1 to 4466)
  AUTHORS   Blouin,J.L., Duriaux-Sail,G., Chen,H., Gos,A., Morris,M.A.,
            Rossier,C. and Antonarakis,S.E.
  TITLE     Mapping of the gene for the p60 subunit of the human chromatin
            assembly factor (CAF1A) to the Down syndrome region of chromosome
            21
  JOURNAL   Genomics 33 (2), 309-312 (1996)
   PUBMED   8660983
REFERENCE   10 (bases 1 to 4466)
  AUTHORS   Kaufman,P.D., Kobayashi,R., Kessler,N. and Stillman,B.
  TITLE     The p150 and p60 subunits of chromatin assembly factor I: a
            molecular link between newly synthesized histones and DNA
            replication
  JOURNAL   Cell 81 (7), 1105-1114 (1995)
   PUBMED   7600578
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL711314.1, BC001143.1,
            BC021218.2 and AP000694.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005441.2.
            
            Summary: Chromatin assembly factor I (CAF-I) is required for the
            assembly of histone octamers onto newly-replicated DNA. CAF-I is
            composed of three protein subunits, p50, p60, and p150. The protein
            encoded by this gene corresponds to the p60 subunit and is required
            for chromatin assembly after replication. The encoded protein is
            differentially phosphorylated in a cell cycle-dependent manner. In
            addition, it is normally found in the nucleus except during
            mitosis, when it is released into the cytoplasm. This protein is a
            member of the WD-repeat HIR1 family and may also be involved in DNA
            repair. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.214318.1,
                                           SRR1660803.173669.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314103.6/ ENSP00000315700.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AL711314.1         6-28
            24-67               BC001143.1         1-44
            68-2278             BC021218.2         1-2211
            2279-4466           AP000694.1         33745-35932
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.12-q22.13"
     gene            1..4466
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /note="chromatin assembly factor 1 subunit B"
                     /db_xref="GeneID:8208"
                     /db_xref="HGNC:HGNC:1911"
                     /db_xref="MIM:601245"
     exon            1..60
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            61..263
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..1817
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /note="human chromatin assembly factor-I p60 subunit;
                     chromatin assembly factor I p60 subunit; CAF-1 subunit B;
                     CAF-I 60 kDa subunit; M-phase phosphoprotein 7; CAF-I p60"
                     /codon_start=1
                     /product="chromatin assembly factor 1 subunit B"
                     /protein_id="NP_005432.1"
                     /db_xref="CCDS:CCDS13644.1"
                     /db_xref="GeneID:8208"
                     /db_xref="HGNC:HGNC:1911"
                     /db_xref="MIM:601245"
                     /translation="MKVITCEIAWHNKEPVYSLDFQHGTAGRIHRLASAGVDTNVRIW
                     KVEKGPDGKAIVEFLSNLARHTKAVNVVRFSPTGEILASGGDDAVILLWKVNDNKEPE
                     QIAFQDEDEAQLNKENWTVVKTLRGHLEDVYDICWATDGNLMASASVDNTAIIWDVSK
                     GQKISIFNEHKSYVQGVTWDPLGQYVATLSCDRVLRVYSIQKKRVAFNVSKMLSGIGA
                     EGEARSYRMFHDDSMKSFFRRLSFTPDGSLLLTPAGCVESGENVMNTTYVFSRKNLKR
                     PIAHLPCPGKATLAVRCCPVYFELRPVVETGVELMSLPYRLVFAVASEDSVLLYDTQQ
                     SFPFGYVSNIHYHTLSDISWSSDGAFLAISSTDGYCSFVTFEKDELGIPLKEKPVLNM
                     RTPDTAKKTKSQTHRGSSPGPRPVEGTPASRTQDPSSPGTTPPQARQAPAPTVIRDPP
                     SITPAVKSPLPGPSEEKTLQPSSQNTKAHPSRRVTLNTLQAWSKTTPRRINLTPLKTD
                     TPPSSVPTSVISTPSTEEIQSETPGDAQGSPPELKRPRLDENKGGTESLDP"
     exon            264..396
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            397..514
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            515..618
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            619..715
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            716..800
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            801..894
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            895..964
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1056
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1198
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1630
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1725
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..4466
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2261..2266
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /note="hexamer: ATTAAA"
     polyA_site      2278
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /note="major polyA site"
     regulatory      4445..4450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
                     /note="hexamer: AATAAA"
     polyA_site      4466
                     /gene="CHAF1B"
                     /gene_synonym="CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60;
                     MPHOSPH7; MPP7"
ORIGIN      
        1 gggaagcggc gcgcgctgcg cgggaggtga cggtgcctct gactgtccgg gtccctccag
       61 cattttgcag ctttctcctg tcttgaagaa gtagaacggt gcccgagaaa cgtttttccc
      121 cttcgagact caggaggatg aaagtcatca cttgtgaaat agcctggcac aacaaggagc
      181 ccgtgtacag cctggacttc cagcatggga cggctgggag gatccacaga ctggcgtctg
      241 ccggcgtgga caccaatgtc aggatctgga aggtagaaaa gggaccagat ggaaaagcca
      301 tcgtggaatt tttgtccaat cttgctcgtc ataccaaagc cgtcaatgtt gtgcgttttt
      361 ctccaactgg ggaaatttta gcatcgggag gagatgatgc tgtcatccta ttgtggaagg
      421 tgaatgataa caaggagccg gagcagatcg cttttcagga tgaggacgag gcccagctga
      481 acaaggagaa ctggacggtt gtgaagactc tgcggggcca cttagaagat gtgtatgata
      541 tttgctgggc aactgatggg aatttaatgg cttctgcctc tgtggataac acagccatca
      601 tatgggatgt cagcaaagga caaaagatat caatttttaa tgaacataaa agttatgtcc
      661 aaggagtaac ctgggaccct ttgggtcaat atgttgctac tctgagctgt gacagggtgc
      721 tgcgagtata cagtatacag aagaagcgtg tggctttcaa tgtttcgaag atgctgtctg
      781 gaataggggc tgaaggagag gcaagaagct accggatgtt tcacgacgac agcatgaagt
      841 ctttcttccg tagactgagt ttcactcccg acggatcttt gcttctcacg ccagctggat
      901 gtgtggaatc tggtgaaaat gtaatgaata ccacttatgt tttctccagg aagaatctta
      961 aaaggcccat cgctcatctt ccatgtcctg gaaaagccac tcttgctgtt cgctgctgtc
     1021 cggtctactt tgaactgagg ccagtggtgg aaacaggtgt ggagctgatg agtctgccct
     1081 accgcctggt gtttgctgtg gcctcggagg attccgtgct tctgtatgac acccagcagt
     1141 ccttcccttt tggttacgtg tctaatatac attaccacac cctcagtgac atttcatggt
     1201 ccagcgatgg tgccttcctg gccatttctt ccacggacgg ttactgctca tttgtgacat
     1261 ttgagaaaga tgaacttgga attcctttga aagagaagcc agttttgaac atgagaactc
     1321 ctgatacagc aaagaaaacc aagagtcaga cacatcgagg gtcttcgcca ggacccagac
     1381 cggtagaggg aacccctgcc agcagaaccc aagaccccag cagccccggc acgactcccc
     1441 ctcaggccag acaggcccca gccccaacag tcatcaggga ccctccctcc atcactcctg
     1501 ctgtcaaaag ccccttgccg gggccttcgg aggagaagac cctgcagccc agtagtcaaa
     1561 acacaaaagc ccacccatcc cggagggtca ctctgaacac actgcaagcc tggagcaaga
     1621 caacaccccg gagaataaac ttaacaccct taaagacgga cactccacca agttctgtac
     1681 caaccagtgt gatttccacc ccttctacag aagaaattca gtcagagacg cctggagacg
     1741 ctcagggcag tcccccagag ctaaagcggc ccagactcga tgaaaacaaa ggaggcacgg
     1801 aaagtctgga cccttgatgg gacctcggct tctgctcgaa gcctaccagg ctcccggtgt
     1861 gtgcagggag acggtaaagc tggaggtgcc tgagaccagg gcttccatgg agcgggacac
     1921 actgtaaatg gatttctata acagaagtga catgtgtact gatttttctc cagaaatatg
     1981 gatgctgttg tattcagtat ccatttttaa cttgggacat gaacgtttta acgtagtaaa
     2041 tcctcttttt gatgagtttc tgaaactgga gcggttcaac gttatccagt gtgaaaatca
     2101 gtgagtcctc cctggcatcc tcgtgaaagt gcacacactt catggaggga ctccttttca
     2161 ataagaatta ggaagatgag aaagtaattt gagattttac tctgtcgaat tttagagtat
     2221 ttgaagtgat tgttagattt cacttctaag gagttgattg attaaacttt ggaaggtagt
     2281 tgtgtttgtt ttctgatttt aaaggtgacg atgcttattc ttttagtaac taagtgaagt
     2341 gaaaaagatg ggtatcaccc atcatcttcc caccagaagt aaccctcgtt aatgttgtag
     2401 tatatttcct tagcattttt gtggatctat ttattctaaa aagagaattg agattttatt
     2461 tcatatgcaa tttggtatct aaataggtta gttttttaga ttggtttttc aaagcaaaaa
     2521 aagtatcttt tttgtttttt gagacagggt cttgctctgt tgcccaggct ggagtgcagt
     2581 ggcgctatct cagctcactg cagcctcgac ctcctgggct cgagcgatcc ttccacctca
     2641 gcttcccgag tagctgggac tacaggcatg tgccaccatg ccatgctaat ttcacacaca
     2701 catgcaccca cacacacaca tatatatgat acatatatat atatatacac acacacacaa
     2761 gtatgtgtgt atatatatat tttttttctt ttttttttga gatggagtct cactctgtcg
     2821 cccaggctgg agtacagtgg cacaatctcg gctcactata acctccgcct cctgggttca
     2881 aacgattctc ctgcctctgc ctcttgagta gctaggatta caggtgtgtg ccaccacatc
     2941 cggctaattt ttatattttt agtagagatg gggttttgcc atgttggcca agctggtctc
     3001 taactcctga cctcagatga tctgcctgcc tcggcctccc aaagtgttgg gattacaggt
     3061 gtgagctact gcacctggcc taattattat atttttaggg gagacggggt ttttccatgt
     3121 tgcccaggct ggtctcgaac tcctggggct caagtaatcc tccctcccca gcctcccaaa
     3181 gtgttgggat tacaggcgtg agacaccatg cctggtcaaa tggtatctgt tttgacgggt
     3241 ggttctcaac tgtagctgca tactgaaatc acctaagagc tgacatacca gtgcctgggt
     3301 tcccgcttcg gaggatttgg cctaatcggt ctggggtatg ttctgaatat taggatttct
     3361 taaagctcct ggagtaattc tgatgcgcag ccaagattga gaaatctaag ggaaaaaact
     3421 ttccctggat acggtctgtt ctggttaaat gcatttccac tgaaagcatt tttttttttt
     3481 tccgagacag agtctcgctc tgtcacccag gctggagtgc agtggcgcaa tctccactca
     3541 ctgcaacctc acctcctagg ttcaagggat tctcctgcct cagcctctcc agtagctggg
     3601 attacaggca cccgccacca cgcccggcta atttttgtat ttttagtaga gatggtgttt
     3661 caccatgttg gtcaggctgg tctccaacgc ctgacctcgt gatctgccca cctcagcctc
     3721 ccaaagtgct gggattacag gcgtgagcca ccgcgcccag ccgaaagcat tttcttgatt
     3781 atctctgccc cgtcactgat tgacatcctc ctttgctggg gccgcggact gaaggacagt
     3841 atgcaggagg tgccatcagc tcttctgaaa agagacagct ttcagtgtca tgtggctggc
     3901 gtgaggtgcg gtttgcagtt gttgatcctg tgcagacaga tgaatgacca tgggtcccct
     3961 gtccttattg agcagcctgc ttcctggcca tacctgggga ggaacagtat taggtttttc
     4021 tgggtgtggt ggctcacacc tgtaatccca gcactttggg aggccaaggt gggtggatca
     4081 cttgaggttg ggagtttgag accagcctgg ccaacatggt ggaaccccat ctctactaaa
     4141 aatacaaaaa ctagccaggc gcggtggcac acacttgtaa tcccagctac tcgggaggct
     4201 gaggcacaag aatcgcttga acccgggagg cggagtttgt agtgagctga gatggtgcca
     4261 ctgtactcca gcctgggcga cagagcgaga ctctgtctca aaaaaaaaaa aaaagagaaa
     4321 aagaagaaca gtattaggtt ttacaaatct gtttcttgtg cattgattgt gggagttggg
     4381 gtgggggacc cctgatggcc tgcctgttct cagccatcat ccttaaatat aaatcaaaat
     4441 tggcaataaa agaccaagtg aagtca
//
