LOCUS       NM_005483               3366 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.
ACCESSION   NM_005483
VERSION     NM_005483.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Chan,F.F., Yuen,V.W., Shen,J., Chin,D.W., Law,C.T., Wong,B.P.,
            Chan,C.Y., Cheu,J.W., Ng,I.O., Wong,C.C. and Wong,C.M.
  TITLE     Inhibition of CAF-1 histone chaperone complex triggers cytosolic
            DNA and dsRNA sensing pathways and induces intrinsic immunity of
            hepatocellular carcinoma
  JOURNAL   Hepatology 80 (2), 295-311 (2024)
   PUBMED   38051950
  REMARK    GeneRIF: Inhibition of CAF-1 histone chaperone complex triggers
            cytosolic DNA and dsRNA sensing pathways and induces intrinsic
            immunity of hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Gopinathan Nair,A., Rabas,N., Lejon,S., Homiski,C., Osborne,M.J.,
            Cyr,N., Sverzhinsky,A., Melendy,T., Pascal,J.M., Laue,E.D.,
            Borden,K.L.B., Omichinski,J.G. and Verreault,A.
  TITLE     Unorthodox PCNA Binding by Chromatin Assembly Factor 1
  JOURNAL   Int J Mol Sci 23 (19), 11099 (2022)
   PUBMED   36232396
  REMARK    GeneRIF: Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3366)
  AUTHORS   Yan,W., Shi,X., Wang,H., Liao,A. and Yang,W.
  TITLE     Aberrant SPOP-CHAF1A ubiquitination axis triggers tumor autophagy
            that endows a therapeutical vulnerability in diffuse large B cell
            lymphoma
  JOURNAL   J Transl Med 20 (1), 296 (2022)
   PUBMED   35773729
  REMARK    GeneRIF: Aberrant SPOP-CHAF1A ubiquitination axis triggers tumor
            autophagy that endows a therapeutical vulnerability in diffuse
            large B cell lymphoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3366)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 3366)
  AUTHORS   Geis,F.K., Sabo,Y., Chen,X., Li,Y., Lu,C. and Goff,S.P.
  TITLE     CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in
            infection
  JOURNAL   Proc Natl Acad Sci U S A 119 (4) (2022)
   PUBMED   35074917
  REMARK    GeneRIF: CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs
            early in infection.
REFERENCE   6  (bases 1 to 3366)
  AUTHORS   Murzina,N., Verreault,A., Laue,E. and Stillman,B.
  TITLE     Heterochromatin dynamics in mouse cells: interaction between
            chromatin assembly factor 1 and HP1 proteins
  JOURNAL   Mol Cell 4 (4), 529-540 (1999)
   PUBMED   10549285
REFERENCE   7  (bases 1 to 3366)
  AUTHORS   Shibahara,K. and Stillman,B.
  TITLE     Replication-dependent marking of DNA by PCNA facilitates
            CAF-1-coupled inheritance of chromatin
  JOURNAL   Cell 96 (4), 575-585 (1999)
   PUBMED   10052459
REFERENCE   8  (bases 1 to 3366)
  AUTHORS   Marheineke,K. and Krude,T.
  TITLE     Nucleosome assembly activity and intracellular localization of
            human CAF-1 changes during the cell division cycle
  JOURNAL   J Biol Chem 273 (24), 15279-15286 (1998)
   PUBMED   9614144
REFERENCE   9  (bases 1 to 3366)
  AUTHORS   Verreault,A., Kaufman,P.D., Kobayashi,R. and Stillman,B.
  TITLE     Nucleosome assembly by a complex of CAF-1 and acetylated histones
            H3/H4
  JOURNAL   Cell 87 (1), 95-104 (1996)
   PUBMED   8858152
REFERENCE   10 (bases 1 to 3366)
  AUTHORS   Kaufman,P.D., Kobayashi,R., Kessler,N. and Stillman,B.
  TITLE     The p150 and p60 subunits of chromatin assembly factor I: a
            molecular link between newly synthesized histones and DNA
            replication
  JOURNAL   Cell 81 (7), 1105-1114 (1995)
   PUBMED   7600578
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011498.7, BQ889365.1, BC052620.1, U20979.1, BG775588.1,
            BM826511.1, H98924.1, BE796132.1 and AA576757.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005483.2.
            
            Summary: Chromatin assembly factor I (CAF1) is a nuclear complex
            consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A)
            subunits that assembles histone octamers onto replicating DNA in
            vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM,
            Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.121232.1,
                                           SRR1660803.107529.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301280.10/ ENSP00000301280.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AC011498.7         34278-34300
            24-28               BQ889365.1         1-5
            29-1397             BC052620.1         5-1373
            1398-2446           U20979.1           1261-2309
            2447-2455           BG775588.1         306-314
            2456-2890           U20979.1           2319-2753
            2891-2936           BM826511.1         516-561
            2937-3017           U20979.1           2800-2880
            3018-3022           H98924.1           348-352             c
            3023-3235           U20979.1           2886-3098
            3236-3319           BE796132.1         564-647
            3320-3366           AA576757.1         2-48                c
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..3366
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /note="chromatin assembly factor 1 subunit A"
                     /db_xref="GeneID:10036"
                     /db_xref="HGNC:HGNC:1910"
                     /db_xref="MIM:601246"
     exon            1..175
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..2994
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /note="chromatin assembly factor I (150 kDa); hp150; CAF-I
                     p150; CAF-1 subunit A; CAF-I 150 kDa subunit; chromatin
                     assembly factor I p150 subunit; CTB-50L17.7"
                     /codon_start=1
                     /product="chromatin assembly factor 1 subunit A"
                     /protein_id="NP_005474.2"
                     /db_xref="CCDS:CCDS32875.1"
                     /db_xref="GeneID:10036"
                     /db_xref="HGNC:HGNC:1910"
                     /db_xref="MIM:601246"
                     /translation="MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNL
                     VPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFL
                     RNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGL
                     LKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCP
                     RKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFP
                     EEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNK
                     LRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREK
                     DEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQ
                     KPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSF
                     LKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENH
                     RPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDM
                     GEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQ
                     PVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLL
                     PLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRL
                     KRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSV
                     PSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDC
                     MIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS"
     exon            176..226
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            227..1083
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1140
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1370
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1431
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1500
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1727
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1896
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1977
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2070
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2326
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..2796
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2893
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..3366
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3197..3202
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /note="hexamer: AATAAA"
     polyA_site      3214
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
     regulatory      3343..3348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /note="hexamer: TATAAA"
     polyA_site      3366
                     /gene="CHAF1A"
                     /gene_synonym="CAF-1; CAF1; CAF1B; CAF1P150; P150"
                     /note="major polyA site"
ORIGIN      
        1 caaatacgag cgcggcggcc gcggcggcag cagcggcgcg ggcgggaggg cgaagagcag
       61 cggccgcctg aggggagccc gcgcctccgc cgcctgagag gaggtcgagc tgccgccggg
      121 gcgatgctgg aggagctgga gtgcggggcg cccggcgcca ggggagccgc cacagccatg
      181 gattgcaaag atagaccagc ttttccagtt aagaagttaa tacaagcccg tctgccgttt
      241 aagcgcctga atcttgtccc aaaggggaaa gccgatgaca tgtcagacga tcagggtact
      301 tctgtgcaaa gtaaaagccc cgatttagag gcctctttgg acaccttgga aaacaactgt
      361 catgtgggtt ctgacataga ctttagaccg aaacttgtca acgggaaggg tcccttagat
      421 aactttttaa gaaatagaat cgaaaccagt attggccaga gcacagtcat cattgatttg
      481 acagaggact cgaatgagca gccagacagt cttgtggacc acaataaact aaattctgaa
      541 gcctctccct ccagggaggc aataaatggc cagcgagaag acactgggga tcagcagggg
      601 ttgttgaagg ccattcagaa cgacaagttg gcatttcctg gagagaccct ttcagacatt
      661 ccttgcaaaa cagaggagga gggtgttggc tgtggaggtg cagggaggag aggcgactcc
      721 caggaatgtt cgccacggag ctgcccggag ctgacgagtg gcccgagaat gtgccccaga
      781 aaggagcagg acagttggag tgaagctggg ggcatcctgt tcaaagggaa ggtgcctatg
      841 gtggtcttgc aggacatctt ggctgtgaga ccaccgcaaa tcaagtccct tccagccaca
      901 ccccaaggca agaacatgac ccctgagagt gaggtgctgg aatctttccc cgaagaagac
      961 tctgtactca gccattcgtc cctgagctct ccctcttcca ccagctcgcc cgaggggccg
     1021 cctgctcccc caaagcagca cagcagtacc agtcccttcc ccacctccac gcccctccgc
     1081 agaataacta agaaattcgt caaaggctct acagagaaga acaagctcag actgcaaaga
     1141 gatcaggagc gtctgggcaa gcagctcaag ttacgtgcag aaagggaaga aaaggagaag
     1201 ctgaaagagg aggccaagcg ggccaaggag gaggccaaga agaagaagga ggaagagaag
     1261 gagcttaagg aaaaggagag gcgggagaag cgggagaagg atgagaagga gaaggcggag
     1321 aagcagcggc tcaaggagga gcggcgcaag gagagacagg aagccctgga ggctaaactt
     1381 gaggaaaaaa ggaaaaagga agaagagaaa cggttaagag aagaagagaa gcgcattaaa
     1441 gcagagaagg ccgaaatcac gaggttcttc cagaaaccaa agactccaca ggcccccaag
     1501 accctggccg gctcctgtgg gaagtttgcc ccctttgaaa ttaaagagca catggtcctg
     1561 gcccctcggc gtcggaccgc tttccatcca gacctctgca gtcagctgga ccagctcctc
     1621 cagcagcaga gcggcgagtt ctccttcttg aaagacctca aaggccggca gcccctgagg
     1681 tccggaccca cgcacgtttc cacccggaat gcagatattt ttaacagtga tgtcgtcatc
     1741 gtggagcgtg ggaagggcga cggtgttccc gagaggagga agtttggcag gatgaagctc
     1801 ctgcagttct gtgagaacca ccggcctgcc tactggggta cctggaataa gaagacggca
     1861 ctcatccgcg cgcgagaccc ctgggcccag gacacgaagc tcctggacta tgaggtggac
     1921 agtgatgagg agtgggaaga agaggagcct ggggagtccc tgtcccacag tgagggggat
     1981 gatgatgacg acatgggaga ggatgaagat gaggacgatg gtttctttgt gccccatggg
     2041 tacctgtctg aggacgaagg tgtgacagag gagtgtgccg accctgagaa ccataaggtc
     2101 cgccagaaac tgaaggccaa ggagtgggac gagttcctgg ctaaggggaa gcgctttcgc
     2161 gtcctgcaac ctgtgaagat cggctgcgtg tgggcggctg acagagactg cgcaggcgat
     2221 gacctgaagg tactgcagca gttcgcagcc tgcttcctgg agaccctgcc ggcccaggag
     2281 gagcagacgc ccaaggcctc caagcgggag aggagagacg agcagatcct ggcccagctg
     2341 ctgccgctcc tgcacggcaa tgtgaacggg agcaaggtca tcatccggga gttccaggag
     2401 cactgccgcc ggggactgct cagcaaccac accggcagcc cgcggagccc ctccaccacc
     2461 tacctgcaca cccccacccc cagcgaggat gccgccatcc cctctaagtc ccggctcaag
     2521 cggctcattt ccgagaactc agtgtatgag aagcggcctg acttcaggat gtgctggtac
     2581 gtgcacccgc aggtgctaca gagcttccag caggagcacc tgcccgtgcc gtgccagtgg
     2641 agctatgtga catcggtgcc ctcggccccc aaagaggaca gtggcagcgt cccctccacg
     2701 gggcccagcc agggcactcc catctcgctg aagaggaagt cagcgggcag catgtgcatc
     2761 acccaattca tgaagaagcg caggcacgac ggccagattg gtgctgaaga catggacggc
     2821 ttccaggcag acacggagga ggaggaagag gaggagggcg actgtatgat cgtggatgtc
     2881 ccggatgctg cggaggtcca agccccgtgt ggagccgctt ccggagctgg gggtggtgtg
     2941 ggggtggaca ccggcaaggc caccctgacc gcgagcccac tgggtgcatc ctgagagcag
     3001 gggtgacgta tgtagaatgc ttagggtgtc ctccccacag agcagatact tgaaccgact
     3061 caattcctgt gtaaagagca ctttgtcctg cttcacggac ctccccaaag tgtgcagagt
     3121 tctatatagg atgctggatt agttcctttg atatttgtaa aaattccccc aagagccgca
     3181 tatgaatctg ccctttaata aagcattatt gagattgctg gcctattggg gaagcctgcg
     3241 ggcacaggag caggcgtgga atccaatact tgtaaatgaa ttgaagcgtc aggaccaccc
     3301 gcctggccac gtgcgcgggc ccctggacct aacgaggcag tgtataaact tattctctag
     3361 ccctga
//
