LOCUS       NM_001267               1705 bp    mRNA    linear   PRI 28-APR-2025
DEFINITION  Homo sapiens chondroadherin (CHAD), mRNA.
ACCESSION   NM_001267
VERSION     NM_001267.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Paracuellos,P., Kalamajski,S., Bonna,A., Bihan,D., Farndale,R.W.
            and Hohenester,E.
  TITLE     Structural and functional analysis of two small leucine-rich repeat
            proteoglycans, fibromodulin and chondroadherin
  JOURNAL   Matrix Biol 63, 106-116 (2017)
   PUBMED   28215822
  REMARK    GeneRIF: crystal structures at ~2.2A resolution of human
            fibromodulin and chondroadherin.
REFERENCE   3  (bases 1 to 1705)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 1705)
  AUTHORS   Haglund,L., Tillgren,V., Onnerfjord,P. and Heinegard,D.
  TITLE     The C-terminal peptide of chondroadherin modulates cellular
            activity by selectively binding to heparan sulfate chains
  JOURNAL   J Biol Chem 288 (2), 995-1008 (2013)
   PUBMED   23172228
  REMARK    GeneRIF: number of cells adhering via their beta(1) integrin
            receptor to collagen type II or chondroadherin was profoundly and
            rapidly enhanced by the addition of the heparin-binding peptide
REFERENCE   5  (bases 1 to 1705)
  AUTHORS   Haglund,L., Tillgren,V., Addis,L., Wenglen,C., Recklies,A. and
            Heinegard,D.
  TITLE     Identification and characterization of the integrin alpha2beta1
            binding motif in chondroadherin mediating cell attachment
  JOURNAL   J Biol Chem 286 (5), 3925-3934 (2011)
   PUBMED   21127050
  REMARK    GeneRIF: chondroadherin interaction with cells may be central for
            maintaining the adult chondrocyte phenotype and cartilage
            homeostasis.
REFERENCE   6  (bases 1 to 1705)
  AUTHORS   Haglund,L., Ouellet,J. and Roughley,P.
  TITLE     Variation in chondroadherin abundance and fragmentation in the
            human scoliotic disc
  JOURNAL   Spine (Phila Pa 1976) 34 (14), 1513-1518 (2009)
   PUBMED   19525844
  REMARK    GeneRIF: Changes in CHAD abundance or structure could be associated
            with the pathologic changes occurring in the scoliotic IVD.
REFERENCE   7  (bases 1 to 1705)
  AUTHORS   Mansson,B., Wenglen,C., Morgelin,M., Saxne,T. and Heinegard,D.
  TITLE     Association of chondroadherin with collagen type II
  JOURNAL   J Biol Chem 276 (35), 32883-32888 (2001)
   PUBMED   11445564
REFERENCE   8  (bases 1 to 1705)
  AUTHORS   Grover,J., Chen,X.N., Korenberg,J.R. and Roughley,P.J.
  TITLE     The structure and chromosome location of the human chondroadherin
            gene (CHAD)
  JOURNAL   Genomics 45 (2), 379-385 (1997)
   PUBMED   9344663
REFERENCE   9  (bases 1 to 1705)
  AUTHORS   Camper,L., Heinegard,D. and Lundgren-Akerlund,E.
  TITLE     Integrin alpha2beta1 is a receptor for the cartilage matrix protein
            chondroadherin
  JOURNAL   J Cell Biol 138 (5), 1159-1167 (1997)
   PUBMED   9281592
REFERENCE   10 (bases 1 to 1705)
  AUTHORS   Larsson,T., Sommarin,Y., Paulsson,M., Antonsson,P., Hedbom,E.,
            Wendel,M. and Heinegard,D.
  TITLE     Cartilage matrix proteins. A basic 36-kDa protein with a restricted
            distribution to cartilage and bone
  JOURNAL   J Biol Chem 266 (30), 20428-20433 (1991)
   PUBMED   1939097
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC021491.16, BC073974.1 and
            BC036360.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001267.2.
            
            Summary: Chondroadherin is a cartilage matrix protein thought to
            mediate adhesion of isolated chondrocytes.  The protein contains 11
            leucine-rich repeats flanked by cysteine-rich regions. The
            chondroadherin messenger RNA is present in chondrocytes at all
            ages. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.918782.1,
                                           SRR3476690.588765.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2155590, SAMEA2467148
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000508540.6/ ENSP00000423812.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC021491.16        140635-140648
            15-645              BC073974.1         1-631
            646-1705            BC036360.1         630-1689
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.33"
     gene            1..1705
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /note="chondroadherin"
                     /db_xref="GeneID:1101"
                     /db_xref="HGNC:HGNC:1909"
                     /db_xref="MIM:602178"
     exon            1..841
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..1147
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /note="chondroadherin proteoglycan; cartilage leucine-rich
                     protein"
                     /codon_start=1
                     /product="chondroadherin precursor"
                     /protein_id="NP_001258.2"
                     /db_xref="CCDS:CCDS11568.1"
                     /db_xref="GeneID:1101"
                     /db_xref="HGNC:HGNC:1909"
                     /db_xref="MIM:602178"
                     /translation="MVRPMLLLSLGLLAGLLPALAACPQNCHCHSDLQHVICDKVGLQ
                     KIPKVSEKTKLLNLQRNNFPVLAANSFRAMPNLVSLHLQHCQIREVAAGAFRGLKQLI
                     YLYLSHNDIRVLRAGAFDDLTELTYLYLDHNKVTELPRGLLSPLVNLFILQLNNNKIR
                     ELRAGAFQGAKDLRWLYLSENALSSLQPGALDDVENLAKFHVDRNQLSSYPSAALSKL
                     RVVEELKLSHNPLKSIPDNAFQSFGRYLETLWLDNTNLEKFSDGAFLGVTTLKHVHLE
                     NNRLNQLPSNFPFDSLETLALTNNPWKCTCQLRGLRRWLEAKASRPDATCASPAKFKG
                     QHIRDTDAFRSCKFPTKRSKKAGRH"
     exon            842..1005
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1151
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1705
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1683..1688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /note="hexamer: TATAAA"
     polyA_site      1705
                     /gene="CHAD"
                     /gene_synonym="SLRR4A"
                     /note="major polyA site"
ORIGIN      
        1 aggactcgcc gctgcccgcg ccccgccgcc gctgctgccc ccagccccgg ccccaggcgt
       61 cccagccatg gtccgcccaa tgctcttgct cagcctcggc ctcctggctg gtctgctgcc
      121 ggcgctggcc gcctgccccc agaactgcca ctgccacagc gacctgcagc acgtcatctg
      181 cgacaaggtg gggctgcaga agatccccaa ggtgtcagag aagaccaagc tgctcaacct
      241 acagcgcaac aacttcccgg tgctggctgc caattcgttc cgggccatgc cgaacctcgt
      301 gtcattgcac ctgcagcact gccagatccg cgaggtggcc gccggtgcct tccgcggcct
      361 caagcaactt atctacttgt acctgtccca taacgacatc cgcgtgctgc gcgcaggtgc
      421 cttcgacgac ctgaccgagc tgacctacct ctacctggac cacaacaagg tcactgagct
      481 gccccggggg ttgctctccc cgctggtcaa cctcttcatc ttgcagctca acaacaacaa
      541 gatccgtgag ctgcgcgcag gcgccttcca gggagccaag gacctgcgct ggctctacct
      601 gtcggaaaac gcgttgagct ccctgcagcc cggggccctg gacgacgtgg agaacctcgc
      661 caaattccac gtggacagga accagctgtc cagctacccc tcagctgccc tgagcaagct
      721 acgggtggtg gaggagctga agctgtccca caaccccctg aaaagcatcc cggacaatgc
      781 cttccagtcc tttggcagat acctggagac cctctggctg gacaacacca acctggagaa
      841 gttctcagat ggtgccttcc tgggtgtaac cacgctgaaa cacgtccatt tggagaacaa
      901 ccgcttgaac cagctaccct ccaacttccc cttcgacagc ctggagaccc tcgcccttac
      961 caataacccc tggaagtgta cctgccagct ccggggcctt cggcggtggc tggaagccaa
     1021 ggcctcccgc ccagatgcca cctgtgcctc acctgccaag ttcaagggcc agcacatccg
     1081 tgacacggac gccttccgca gctgcaagtt ccccaccaag aggtccaaga aagctggccg
     1141 ccattaaaca ggttctgacc cagccagtcc tggtgactgg cctctgcctt ccaccggaga
     1201 gactactgac cttctcacct ccacccatac cttctcccca cagcctctgc tgatgcacag
     1261 agctgcctac acctagacac gtcctggcag ggggcctcgg gcactccact accaacccag
     1321 ctccacccag cagtgtcctg gggagaagga aggctgagcc tctccccagc cttcatgcct
     1381 tccccaccct ccagctcctc ttggagaagc tgttgctgag accccccccc caagtcaatc
     1441 agaaccacaa caggttggtc atcaggatgg ccaccctccc aggatcatcc ttcctctgtt
     1501 cctctttccc tgccacgtgg aaacaatcat cagatccttg ccccacccct tgcttccaga
     1561 aagggtttta aagcccatgc cccaactctg ccagccccca cctgccagga cgttctagca
     1621 ggacatcggt gctttgctgt ccatcttccc atgctgcaat ttcttcctga gatttctata
     1681 aatataaatg tatgtatgta ttata
//
