LOCUS       NM_032866               7214 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens cingulin like 1 (CGNL1), transcript variant 2, mRNA.
ACCESSION   NM_032866
VERSION     NM_032866.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 7214)
  AUTHORS   Chrifi,I., Hermkens,D., Brandt,M.M., van Dijk,C.G.M.,
            Burgisser,P.E., Haasdijk,R., Pei,J., van de Kamp,E.H.M., Zhu,C.,
            Blonden,L., Kros,J.M., Duncker,D.J., Duckers,H.J. and Cheng,C.
  TITLE     Cgnl1, an endothelial junction complex protein, regulates GTPase
            mediated angiogenesis
  JOURNAL   Cardiovasc Res 113 (14), 1776-1788 (2017)
   PUBMED   29016873
  REMARK    GeneRIF: demonstrate in a postnatal retinal vascular development
            model in mice that Cgnl1 function is crucial for sustaining
            neovascular growth and stability
REFERENCE   4  (bases 1 to 7214)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 7214)
  AUTHORS   Pulimeno,P., Paschoud,S. and Citi,S.
  TITLE     A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and
            adherens junctions of epithelial cells
  JOURNAL   J Biol Chem 286 (19), 16743-16750 (2011)
   PUBMED   21454477
  REMARK    GeneRIF: ZO-1 and PLEKHA7 are paracingulin-interacting proteins
            that are involved in its recruitment to epithelial tight and
            adherens junctions, respectively
REFERENCE   6  (bases 1 to 7214)
  AUTHORS   Bandyopadhyay,S., Chiang,C.Y., Srivastava,J., Gersten,M., White,S.,
            Bell,R., Kurschner,C., Martin,C., Smoot,M., Sahasrabudhe,S.,
            Barber,D.L., Chanda,S.K. and Ideker,T.
  TITLE     A human MAP kinase interactome
  JOURNAL   Nat Methods 7 (10), 801-805 (2010)
   PUBMED   20936779
REFERENCE   7  (bases 1 to 7214)
  AUTHORS   Guillemot,L., Paschoud,S., Jond,L., Foglia,A. and Citi,S.
  TITLE     Paracingulin regulates the activity of Rac1 and RhoA GTPases by
            recruiting Tiam1 and GEF-H1 to epithelial junctions
  JOURNAL   Mol Biol Cell 19 (10), 4442-4453 (2008)
   PUBMED   18653465
  REMARK    GeneRIF: Paracingulin regulates the activity of Rac1 and RhoA
            GTPases by recruiting Tiam1 and GEF-H1 to epithelial junctions
REFERENCE   8  (bases 1 to 7214)
  AUTHORS   Demura,M., Martin,R.M., Shozu,M., Sebastian,S., Takayama,K.,
            Hsu,W.T., Schultz,R.A., Neely,K., Bryant,M., Mendonca,B.B.,
            Hanaki,K., Kanzaki,S., Rhoads,D.B., Misra,M. and Bulun,S.E.
  TITLE     Regional rearrangements in chromosome 15q21 cause formation of
            cryptic promoters for the CYP19 (aromatase) gene
  JOURNAL   Hum Mol Genet 16 (21), 2529-2541 (2007)
   PUBMED   17584767
REFERENCE   9  (bases 1 to 7214)
  AUTHORS   Ohnishi,H., Nakahara,T., Furuse,K., Sasaki,H., Tsukita,S. and
            Furuse,M.
  TITLE     JACOP, a novel plaque protein localizing at the apical junctional
            complex with sequence similarity to cingulin
  JOURNAL   J Biol Chem 279 (44), 46014-46022 (2004)
   PUBMED   15292197
  REMARK    GeneRIF: This paper studied the mouse JACOP protein and found that
            the JACOP protein has structural and functional similarities to
            cingulin.
REFERENCE   10 (bases 1 to 7214)
  AUTHORS   Shozu,M., Sebastian,S., Takayama,K., Hsu,W.T., Schultz,R.A.,
            Neely,K., Bryant,M. and Bulun,S.E.
  TITLE     Estrogen excess associated with novel gain-of-function mutations
            affecting the aromatase gene
  JOURNAL   N Engl J Med 348 (19), 1855-1865 (2003)
   PUBMED   12736278
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC415862.1, AY274808.1 and
            AA661765.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_032866.4.
            
            Summary: This gene encodes a member of the cingulin family. The
            encoded protein localizes to both adherens and tight cell-cell
            junctions and mediates junction assembly and maintenance by
            regulating the activity of the small GTPases RhoA and Rac1.
            Heterozygous chromosomal rearrangements resulting in association of
            the promoter for this gene with the aromatase gene are a cause of
            aromatase excess syndrome. Alternatively spliced transcript
            variants have been observed for this gene. [provided by RefSeq, Nov
            2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY274808.1, BC118918.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281282.6/ ENSP00000281282.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                DC415862.1         1-53
            54-121              DC415862.1         55-122
            122-7212            AY274808.1         120-7210
            7213-7214           AA661765.1         25-26               c
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.3"
     gene            1..7214
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /note="cingulin like 1"
                     /db_xref="GeneID:84952"
                     /db_xref="HGNC:HGNC:25931"
                     /db_xref="MIM:607856"
     exon            1..63
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            64..1680
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..3987
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /note="paracingulin; junction-associated coiled-coil
                     protein"
                     /codon_start=1
                     /product="cingulin-like protein 1"
                     /protein_id="NP_116255.2"
                     /db_xref="CCDS:CCDS10161.1"
                     /db_xref="GeneID:84952"
                     /db_xref="HGNC:HGNC:25931"
                     /db_xref="MIM:607856"
                     /translation="MELYFGEYQHVQQEYGVHLRLASDDTQKSRSSQNSKAGSYGVSI
                     RVQGIDGHPYIVLNNTERCLAGTSFSENGPPFPPPVINNLPLHSSNGSVPKENSEELQ
                     LPENPYAQPSPIRNLKQPLLHEGKNGVLDRKDGSVKPSHLLNFQRHPELLQPYDPEKN
                     ELNLQNHQPSESNWLKTLTEEGINNKKPWTCFPKPSNSQPTSPSLEDPAKSGVTAIRL
                     CSSVVIEDPKKQTSVCVNVQSCTKERVGEEALFTSGRPLTAHSPHAHPETKKTRPDVL
                     PFRRQDSAGPVLDGARSRRSSSSSTTPTSANSLYRFLLDDQECAIHADNVNRHENRRY
                     IPFLPGTGRDIDTGSIPGVDQLIEKFDQKPGLQRRGRSGKRNRINTDDRKRSRSVDSA
                     FPFGLQGNSEYLIEFSRNLGKSSEHLLRPSQVCPQRPLSQERRGKQSVGRTFAKLQGA
                     AHGASCAHSRPPQPNIDGKVLETEGSQESTVIRAPSLGAQSKKEEEVKTATATLMLQN
                     RATATSPDSGAKKISVKTFPSASNTQATPDLLKGQQELTQQTNEETAKQILYNYLKEG
                     STDNDDATKRKVNLVFEKIQTLKSRAAGSAQGNNQACNSTSEVKDLLEQKSKLTIEVA
                     ELQRQLQLEVKNQQNIKEERERMRANLEELRSQHNEKVEENSTLQQRLEESEGELRKN
                     LEELFQVKMEREQHQTEIRDLQDQLSEMHDELDSAKRSEDREKGALIEELLQAKQDLQ
                     DLLIAKEEQEDLLRKRERELTALKGALKEEVSSHDQEMDKLKEQYDAELQALRESVEE
                     ATKNVEVLASRSNTSEQDQAGTEMRVKLLQEENEKLQGRSEELERRVAQLQRQIEDLK
                     GDEAKAKETLKKYEGEIRQLEEALVHARKEEKEAVSARRALENELEAAQGNLSQTTQE
                     QKQLSEKLKEESEQKEQLRRLKNEMENERWHLGKTIEKLQKEMADIVEASRTSTLELQ
                     NQLDEYKEKNRRELAEMQRQLKEKTLEAEKSRLTAMKMQDEMRLMEEELRDYQRAQDE
                     ALTKRQLLEQTLKDLEYELEAKSHLKDDRSRLVKQMEDKVSQLEMELEEERNNSDLLS
                     ERISRSREQMEQLRNELLQERAARQDLECDKISLERQNKDLKSRIIHLEGSYRSSKEG
                     LVVQMEARIAELEDRLESEERDRANLQLSNRRLERKVKELVMQVDDEHLSLTDQKDQL
                     SLRLKAMKRQVEEAEEEIDRLESSKKKLQRELEEQMDMNEHLQGQLNSMKKDLRLKKL
                     PSKVLDDMDDDDDLSTDGGSLYEAPVSYTFSKDSTVASQI"
     exon            1681..1775
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1881
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1983
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2132
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2268
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2481
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2688
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2793
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2946
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            2947..3117
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3118..3279
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3280..3369
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3370..3453
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3578
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..3687
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..3851
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     exon            3852..7214
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7190..7195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /note="hexamer: AATAAA"
     polyA_site      7214
                     /gene="CGNL1"
                     /gene_synonym="JACOP; PCING"
                     /note="major polyA site"
ORIGIN      
        1 gcactccggc cgggctctgc tggctgcggc gggagctgga cgcgggagga ggcggcggcg
       61 gcgctggaac agtgaaccat ggagctgtat ttcggtgaat atcaacatgt gcagcaggaa
      121 tatggggtcc atctgagact cgcaagtgat gatacccaaa aatcaaggag ttcccagaac
      181 tccaaggcag gctcctacgg tgtcagtatt cgggtccagg gaattgatgg tcacccctat
      241 attgtcctga ataacacaga acggtgccta gcaggcacat cgttttctga aaatgggcca
      301 ccctttccac ctccagtgat aaataacctg cctctacatt ccagcaatgg ttctgtgcca
      361 aaggagaaca gtgaagaact tcagcttcca gaaaacccat acgcccagcc tagcccaata
      421 agaaacctga aacagcccct gctccatgag ggcaagaatg gagttctaga tcgcaaagac
      481 gggtctgtga agccatctca cctgctgaac tttcagaggc atccagagct tttgcaaccc
      541 tatgaccctg aaaagaatga gttgaattta caaaatcacc agccttctga gagtaattgg
      601 ctaaaaacgt tgacagaaga aggcatcaac aataagaagc cttggacttg ctttcccaaa
      661 cctagcaatt cccagcctac cagtccctcc ttggaagacc cggccaaatc tggtgtgaca
      721 gctattcgtt tatgcagctc cgtggtcata gaggacccca aaaagcagac ctcagtgtgt
      781 gtaaacgttc agagctgcac caaggagagg gtgggagagg aggccctttt cactagcggg
      841 aggcccctga ctgcccacag cccacatgcc caccctgaaa ccaagaaaac caggccagat
      901 gttcttccct tccggcgaca ggattcagcg ggacccgtcc tggatggagc tcggtcccgg
      961 aggtcctcct cgtcatccac aactcccacg tcagccaact ctttgtacag gtttttactg
     1021 gatgatcagg aatgtgccat ccatgccgac aacgtcaatc gtcatgaaaa cagaaggtat
     1081 attcccttcc tgccaggaac tggacgggat attgatacag gatcaattcc tggtgtggat
     1141 cagttaattg aaaaatttga tcaaaaacct gggcttcaga gaagaggaag gtctgggaag
     1201 cgaaacagaa ttaatacaga tgacaggaaa agatccagaa gcgtggatag cgcctttcct
     1261 tttggcctcc aagggaactc ggagtacctg attgaattca gtaggaactt gggcaagtca
     1321 agcgaacacc tcctccggcc ttcccaggtg tgcccgcagc ggccactgtc tcaggagcgc
     1381 cgtgggaaac agagcgtggg ccgcaccttt gcaaagctgc agggagcagc gcacggggct
     1441 tcatgtgccc actccaggcc tccccagccg aacatagatg ggaaagttct ggaaaccgaa
     1501 ggtagtcagg aaagtacagt gatccgtgcg ccctcccttg gtgcacagag taaaaaggag
     1561 gaggaggtga aaacagccac cgctacgctg atgttacaga accgggcaac agcaacttcg
     1621 cctgattctg gtgccaagaa aatttccgtg aagacatttc cttcggcctc aaatactcag
     1681 gccacaccgg atctcttaaa gggccagcaa gagctcactc agcaaaccaa tgaggagaca
     1741 gctaagcaga ttctctacaa ttacctcaaa gaaggaagca ctgataatga cgatgctact
     1801 aaaaggaaag tcaacttggt ctttgagaaa atccagacct taaagtctcg agcagctggg
     1861 agcgcccaag gaaataacca agcttgtaat tccacatctg aagtcaaaga tctattggaa
     1921 cagaaaagca agttgaccat agaagtggct gaacttcaga gacagcttca actggaagtc
     1981 aagaatcaac agaacattaa agaagagaga gagaggatga gagcaaacct agaagagctc
     2041 cgaagccaac acaacgaaaa ggtggaggag aactccacat tgcagcaacg actggaagaa
     2101 agtgaagggg agctccggaa gaatctggag gagctattcc aggtgaagat ggaacgggag
     2161 cagcatcaga ctgagatcag ggatctccag gaccagctct cagaaatgca cgatgaactg
     2221 gacagtgcaa agcgatcgga ggacagggag aagggagctc tgattgagga gctcttacag
     2281 gcaaaacagg atcttcaaga tctgctgatt gccaaagagg agcaagaaga cctcttgaga
     2341 aagcgagagc gtgaactcac cgccctgaag ggagccctga aagaagaggt ttccagccat
     2401 gatcaggaga tggacaagct gaaggagcaa tatgatgctg agttgcaggc cctgagggag
     2461 agtgtggaag aagcaaccaa gaatgtcgag gtcttggcga gcaggagcaa cacttcagag
     2521 caagaccagg cggggactga aatgcgcgtg aagcttctgc aggaggagaa tgagaagctg
     2581 cagggaagaa gcgaagagct ggagcggaga gttgctcagc ttcaaaggca gatcgaggac
     2641 ctgaaaggcg atgaagccaa ggcgaaggaa acgctgaaga agtacgaggg agaaatacga
     2701 cagttagagg aggcccttgt gcacgccaga aaggaagaaa aagaagctgt gtcagccaga
     2761 agggccctgg agaatgaact ggaggctgct cagggaaatc tgagtcagac tacccaggag
     2821 cagaagcagt tgtctgagaa gctcaaagag gagagtgagc agaaggagca gctaagaagg
     2881 ttgaagaacg agatggagaa tgagcggtgg cacctgggca aaaccattga gaaactgcag
     2941 aaggagatgg cagacattgt tgaggcctcc cgtacctcaa ccctggagct ccagaaccag
     3001 ctggatgagt ataaggagaa aaaccgcagg gagctcgcag aaatgcaaag acagttgaag
     3061 gagaaaacgc tggaggcaga aaagtcccga ctgacagcca tgaaaatgca ggatgagatg
     3121 cgtctgatgg aggaagagtt acgggactac cagagagctc aggatgaagc actcacaaaa
     3181 aggcagcttc tggagcagac gctgaaggac ctggagtatg agctggaagc caagagtcac
     3241 ctcaaagatg accgcagcag gctggtcaag cagatggagg acaaggtgtc tcaactggag
     3301 atggaactgg aagaagagag aaacaactca gatttgctgt ctgagaggat cagtaggagc
     3361 agggaacaga tggagcagtt gaggaatgag ctacttcagg agagagctgc gagacaagac
     3421 ttggagtgcg acaagatttc cctggagagg cagaacaagg acttaaagag ccggattatc
     3481 cacctggaag gttcctacag gtccagcaaa gaggggctgg ttgtgcagat ggaggccagg
     3541 atcgcggagc tggaggaccg cctggagagt gaggagaggg atcgggccaa tcttcagctc
     3601 agcaaccggc ggctggagcg gaaagtgaag gagctggtga tgcaggtgga tgatgagcac
     3661 ctgtcattga ctgatcagaa ggaccagctg agcttgcgtt tgaaagccat gaagcggcag
     3721 gtggaggagg ctgaggagga aatcgacaga ctggaaagtt ctaaaaagaa gctgcagagg
     3781 gagctggagg agcagatgga catgaatgag catctgcagg ggcagctcaa ctccatgaag
     3841 aaggacttaa gactgaagaa gctgccgagt aaagtgctgg atgacatgga tgacgacgat
     3901 gacctcagca cggatggggg aagcctctat gaggcgcctg tgagctacac attctccaag
     3961 gacagcaccg tcgccagcca gatctgagtg ctctcccggg ctgtggaagt acctgtcatt
     4021 cctgcaggag ctgcagccac ccaaagtggg aggcagggag gggagcatct gtctgccact
     4081 gagaccaatc acagcctctt tgcacagcat gccagctcct cagtgttaca ttcctcgcca
     4141 gggtctctca gtgggtcttc gacagagagc ttttgcagtt taaaatgttg ggatgcctga
     4201 ggaccaggag ccaccaccca ctggggtgtt gtgagagata cactttggta ggctgaggcc
     4261 cctgttccac agccactatt tgcattgctg tccctgcccc ctcatcactc cccctgccaa
     4321 gacaaagggt ccagaaggct aggacttact taatagcact gtgcagggag gaaaccatgt
     4381 atagcttgta catattttaa accagacctt ctcacgaact gctgctgttg ggtttggaat
     4441 gtgatggagg cttggtaggg tggctggttt gtaaagttca tgcatctata ttgagtattc
     4501 tttttttttt tttttgagat ggagtttcat tcttgttgcc gaggctggag tgcaatggcg
     4561 caaccttggc tcactgcaac ctctgcctcc ctggttcaag tgattctcct gcctcatcct
     4621 cccaagaagc tgagattaca gatgaccacc accactcctg gctaattttt tgtatttagt
     4681 agagacgggg tttcaccatg ttggtcaggc tggtcttgaa ctcctgacct caggtgatcc
     4741 acctgcctca gcctcccaaa gtgctgagat tacaggcatg agccaccacg cccagccatt
     4801 gagtattctt ttttaaaata tgaagctatt agattttaag tgttgaggag cagaggtgag
     4861 aagagagaga gagagagaaa gtcaaataaa gtcaaagacc agtttggaaa ccaaagttgg
     4921 aaaagaagaa ttgtagtggt catggacagc taagactgag catagcagtt cacatggtct
     4981 gggtaaacaa gtcactggca caggggaaag ccagaaggtt gcttgtgtct gtgactgcag
     5041 ctatggcctt gtttgcttag tttatctgga attctgttat aatttctgaa ctgttctgag
     5101 acctgttccc agtcactgat tgcaatttct tttacacagg ttgggtaggg agagttgtag
     5161 gtaaggcctt aaacaaatga ggccgtttct ggaaggatag ggctgtgtgg tcatgtccca
     5221 cagcaagctt ttgggatcgg gggaataaga gggtatgggg gaaaggttca tagacttagg
     5281 tgtgagaatg aggtgggagg tgtctggtaa ggtcatgggg gaagggagat gagagaggag
     5341 ataggcagat gtgggtatgg ggcccagtgt gagaaggaag acagagaagt tcttgaaagc
     5401 tgggggatgt gtgggcaaga ggtggattga gcaggatctc agggttatgt cgaggaaggg
     5461 aagaaagaaa cagaagtcag gcagaggcgt gtctgtgcaa actggaggac tccttccaac
     5521 ttcctggaac aaagcgacat cgccagcagc aggctggcag ggagtggttg aggtctgggg
     5581 gccaggccac atggctggag aggaatttca gggagcatag catcgggagg atgctacata
     5641 tccagggact ccctcctggc cttcctgtgt cacttcacaa tgtgacagtc cccaggcatg
     5701 cccacaggcc aggcccagtc acagctgggc ttccacaggt cagaatatgg gacaggacat
     5761 ggtcagggag aggaccctgt gagttggtga catatatgtg gcagagatgg tagtgttcag
     5821 ctgcttcagc agtgtcccca accatcccaa tagggtgggg gcctgtgggc cagaggactc
     5881 catgacagta gccatggagt cccacctgtg ccaggtcaga gcccacccgg caggtttcag
     5941 agtggtcagc ccagtttaag ggtgggagtc tggagccaga cagccaagcc ccttccacat
     6001 ctacagggtc actgtcatcc tcaccacagc caggggtgat tctcacctct gcctgcttat
     6061 gtggggtgtg gccgattcct ccctttagtc attcattcat ttagcacata tttattgggc
     6121 atcggtgatg tgtcaggcac agtccttggc acacagaata cagcagtgaa ttaaacaggc
     6181 aaaaattcct cccctcctgg agctcagtgt caggaaaggg ggtgataaat gacaatcaaa
     6241 tcgacatgga ggttgagcac ctctgtactc agccctctgt ctgtccttct gaggacgcaa
     6301 caagcctggt tccctagagc tcactttgac cctcaatatg ttcataattt ccctgctgct
     6361 tctcccatcg ttagacatct gccagtaagc ctgctgagag aagcctcagc ccactcatac
     6421 ctagagacgc tgctggggaa ggtcagcaca cccaagaact ttgggagtgt gtgtctgttt
     6481 tggtttgtga gaaagtagct gcccctcatt ctggccagtt ctttccagta gccatggggc
     6541 tggctacaag aagcaaaact gcccatgcaa agagaacaca ggtcactttt caaaacactt
     6601 gcatcattac cctaaaaata ggctgttatg cgttttgaca attgcgctat cctacactct
     6661 gagggagttg gcaacgcatg cggtatagtg gagtgggaag agttgagact gaaagttaag
     6721 agaagggttt tcaagtcccg gctcatctca ccccagtgac tttgacagtg tgctcactct
     6781 cagagccatg gattacctgg aacaaagtgc agattagtgc ccacggtcct ttccaggtat
     6841 aacatgctat cgttctttaa ttttattata tatacatact tttcttctgt tttttgcttt
     6901 aagaactaac cccgatcaag agtattttct ttagctagct taaaaagaaa acatatattt
     6961 tatgtaaaaa cacatacatg gccaaatgca atactgaaga gacaatcatc ctgtatcttt
     7021 tatggttttt cctgcttctg agtgtcgttc tacaatgccc gttgacttat tccattgcgt
     7081 ctctgcttta gggtatggtt tggtcgcttt ggttgtcatg agaaagagac attctccttg
     7141 cagctacctc catgtgagtg aattcctgga taaagcaaga tttcctttca ataaacgctg
     7201 tcacccaatg ggaa
//
