LOCUS       NM_020770               5101 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens cingulin (CGN), mRNA.
ACCESSION   NM_020770
VERSION     NM_020770.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ishii,D., Shindo,Y., Arai,W., Konno,T., Kohno,T., Honda,K.,
            Miyajima,M., Watanabe,A. and Kojima,T.
  TITLE     The Roles and Regulatory Mechanisms of Tight Junction Protein
            Cingulin and Transcription Factor Forkhead Box Protein O1 in Human
            Lung Adenocarcinoma A549 Cells and Normal Lung Epithelial Cells
  JOURNAL   Int J Mol Sci 25 (3), 1411 (2024)
   PUBMED   38338691
  REMARK    GeneRIF: The Roles and Regulatory Mechanisms of Tight Junction
            Protein Cingulin and Transcription Factor Forkhead Box Protein O1
            in Human Lung Adenocarcinoma A549 Cells and Normal Lung Epithelial
            Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Zhu,G.J., Huang,Y., Zhang,L., Yan,K., Qiu,C., He,Y., Liu,Q.,
            Zhu,C., Morin,M., Moreno-Pelayo,M.A., Zhu,M.S., Cao,X., Zhou,H.,
            Qian,X., Xu,Z., Chen,J., Gao,X. and Wan,G.
  TITLE     Cingulin regulates hair cell cuticular plate morphology and is
            required for hearing in human and mouse
  JOURNAL   EMBO Mol Med 15 (11), e17611 (2023)
   PUBMED   37691516
  REMARK    GeneRIF: Cingulin regulates hair cell cuticular plate morphology
            and is required for hearing in human and mouse.
REFERENCE   3  (bases 1 to 5101)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 5101)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5101)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   6  (bases 1 to 5101)
  AUTHORS   D'Atri,F., Nadalutti,F. and Citi,S.
  TITLE     Evidence for a functional interaction between cingulin and ZO-1 in
            cultured cells
  JOURNAL   J Biol Chem 277 (31), 27757-27764 (2002)
   PUBMED   12023291
REFERENCE   7  (bases 1 to 5101)
  AUTHORS   Wolburg,H. and Lippoldt,A.
  TITLE     Tight junctions of the blood-brain barrier: development,
            composition and regulation
  JOURNAL   Vascul Pharmacol 38 (6), 323-337 (2002)
   PUBMED   12529927
  REMARK    Review article
REFERENCE   8  (bases 1 to 5101)
  AUTHORS   Citi,S., D'Atri,F. and Parry,D.A.
  TITLE     Human and Xenopus cingulin share a modular organization of the
            coiled-coil rod domain: predictions for intra- and intermolecular
            assembly
  JOURNAL   J Struct Biol 131 (2), 135-145 (2000)
   PUBMED   11042084
  REMARK    GeneRIF: Cingulin (KIAA1319 protein) is localized in the
            cytoplasmic domain of vertebrate epithelial tight junctions, and it
            interacts with other tight junction proteins such as ZO-1, and with
            the actomyosin cytoskeleton.
REFERENCE   9  (bases 1 to 5101)
  AUTHORS   Bazzoni,G., Martinez-Estrada,O.M., Orsenigo,F., Cordenonsi,M.,
            Citi,S. and Dejana,E.
  TITLE     Interaction of junctional adhesion molecule with the tight junction
            components ZO-1, cingulin, and occludin
  JOURNAL   J Biol Chem 275 (27), 20520-20526 (2000)
   PUBMED   10877843
REFERENCE   10 (bases 1 to 5101)
  AUTHORS   Cordenonsi,M., D'Atri,F., Hammar,E., Parry,D.A., Kendrick-Jones,J.,
            Shore,D. and Citi,S.
  TITLE     Cingulin contains globular and coiled-coil domains and interacts
            with ZO-1, ZO-2, ZO-3, and myosin
  JOURNAL   J Cell Biol 147 (7), 1569-1582 (1999)
   PUBMED   10613913
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CN359389.1, AF263462.1, AL365436.26, BC152445.1, BP331021.1 and
            BC146657.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020770.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC152445.1, AF263462.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000271636.12/ ENSP00000271636.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                CN359389.1         9-18
            11-29               AF263462.1         13-31
            30-30               AL365436.26        4523-4523
            31-97               AF263462.1         33-99
            98-3911             BC152445.1         72-3885
            3912-3958           BC152445.1         3887-3933
            3959-4005           BP331021.1         536-582
            4006-4564           AF263462.1         4015-4573
            4565-5101           BC146657.1         4846-5382
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     gene            1..5101
                     /gene="CGN"
                     /note="cingulin"
                     /db_xref="GeneID:57530"
                     /db_xref="HGNC:HGNC:17429"
                     /db_xref="MIM:609473"
     exon            1..129
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            130..1016
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..3755
                     /gene="CGN"
                     /codon_start=1
                     /product="cingulin"
                     /protein_id="NP_065821.1"
                     /db_xref="CCDS:CCDS999.1"
                     /db_xref="GeneID:57530"
                     /db_xref="HGNC:HGNC:17429"
                     /db_xref="MIM:609473"
                     /translation="MEQAPNMAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAK
                     DARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPE
                     NPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELA
                     PKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSR
                     LPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQS
                     FEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVR
                     RKVSLVLEKMQPLVMVSSGSTKAVAGQGELTRKVEELQRKLDEEVKKRQKLEPSQVGL
                     ERQLEEKTEECSRLQELLERRKGEAQQSNKELQNMKRLLDQGEDLRHGLETQVMELQN
                     KLKHVQGPEPAKEVLLKDLLETRELLEEVLEGKQRVEEQLRLRERELTALKGALKEEV
                     ASRDQEVEHVRQQYQRDTEQLRRSMQDATQDHAVLEAERQKMSALVRGLQRELEETSE
                     ETGHWQSMFQKNKEDLRATKQELLQLRMEKEEMEEELGEKIEVLQRELEQARASAGDT
                     RQVEVLKKELLRTQEELKELQAERQSQEVAGRHRDRELEKQLAVLRVEADRGRELEEQ
                     NLQLQKTLQQLRQDCEEASKAKMVAEAEATVLGQRRAAVETTLRETQEENDEFRRRIL
                     GLEQQLKETRGLVDGGEAVEARLRDKLQRLEAEKQQLEEALNASQEEEGSLAAAKRAL
                     EARLEEAQRGLARLGQEQQTLNRALEEEGKQREVLRRGKAELEEQKRLLDRTVDRLNK
                     ELEKIGEDSKQALQQLQAQLEDYKEKARREVADAQRQAKDWASEAEKTSGGLSRLQDE
                     IQRLRQALQASQAERDTARLDKELLAQRLQGLEQEAENKKRSQDDRARQLKGLEEKVS
                     RLETELDEEKNTVELLTDRVNRGRDQVDQLRTELMQERSARQDLECDKISLERQNKDL
                     KTRLASSEGFQKPSASLSQLESQNQLLQERLQAEEREKTVLQSTNRKLERKVKELSIQ
                     IEDERQHVNDQKDQLSLRVKALKRQVDEAEEEIERLDGLRKKAQREVEEQHEVNEQLQ
                     ARIKSLEKDSWRKASRSAAESALKNEGLSSDEEFDSVYDPSSIASLLTESNLQTSSC"
     exon            1017..1117
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1187
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1283
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1411
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1544
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1757
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1906
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2039
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2249
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2456
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2714
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2885
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..3047
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..3137
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3138..3221
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3222..3340
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3449
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3613
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     exon            3614..5101
                     /gene="CGN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5082..5087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CGN"
                     /note="hexamer: ATTACA"
     polyA_site      5101
                     /gene="CGN"
                     /note="major polyA site"
ORIGIN      
        1 agggagctcc gaggacgagg gggagggccg gagctgcgcg tgctgctttg cccgagcccg
       61 agcccgagcc cgagcccgag cccgagcccg agcccgaacg caagcctggg agcgcggagc
      121 ccggctaggg actcctccta tttatggagc aggcacccaa catggctgag ccccggggcc
      181 ccgtagacca tggagtccag attcgcttca tcacagagcc agtgagtggt gcagagatgg
      241 gcactctacg tcgaggtgga cgacgcccag ctaaggatgc aagagccagt acctacgggg
      301 ttgctgtgcg tgtgcaggga atcgctgggc agccctttgt ggtgctcaac agtggggaga
      361 aaggcggtga ctcctttggg gtccaaatca agggggccaa tgaccaaggg gcctcaggag
      421 ctctgagctc agatttggaa ctccctgaga acccctactc tcaggtcaag ggatttcctg
      481 ccccctcgca gagcagcaca tctgatgagg agcctggggc ctactggaat ggaaagctac
      541 tccgttccca ctcccaggcc tcactggcag gccctggccc agtggatcct agtaacagaa
      601 gcaacagcat gctggagcta gccccgaaag tggcttcccc aggtagcacc attgacactg
      661 ctcccctgtc ttcagtggac tcactcatca acaagtttga cagtcaactt ggaggccagg
      721 cccggggtcg gactggccgc cgaacacgga tgctaccccc tgaacagcgc aaacggagca
      781 agagcctgga cagccgcctc ccacgggaca cctttgagga acgggagcgc cagtccacca
      841 accactggac ctctagcaca aaatatgaca accatgtggg cacttcgaag cagccagccc
      901 agagccagaa cctgagtcct ctcagtggct ttagccgttc tcgtcagact caggactggg
      961 tccttcagag ttttgaggag ccgcggagga gtgcacagga ccccaccatg ctgcagttca
     1021 aatcaactcc agacctcctt cgagaccagc aggaggcagc cccaccaggc agtgtggacc
     1081 atatgaaggc caccatctat ggcatcctga gggagggaag ctcagaaagt gaaacctctg
     1141 tgaggaggaa ggttagtttg gtgctggaga agatgcagcc tctagtgatg gtttcttctg
     1201 gttctactaa ggccgtggca gggcagggtg agcttacccg aaaagtggag gagctacagc
     1261 gaaagctgga tgaagaggtg aagaagcggc agaagctaga gccatcccaa gttgggctgg
     1321 agcggcagct ggaggagaaa acagaagagt gcagccgact gcaggagctg ctggagagga
     1381 ggaaggggga ggcccagcag agcaacaagg agctccagaa catgaagcgc ctcttggacc
     1441 agggtgaaga tttacgacat gggctggaga cccaggtgat ggagctgcag aacaagctga
     1501 aacatgtcca gggtcctgag cctgctaagg aggtgttact gaaggacctg ttagagaccc
     1561 gggaacttct ggaagaggtc ttggagggga aacagcgagt agaggagcag ctgaggctgc
     1621 gggagcggga gttgacagcc ctgaaggggg ccctgaaaga ggaggtagcc tcccgtgacc
     1681 aggaggtgga acatgtccgg cagcagtacc agcgagacac agagcagctc cgcaggagca
     1741 tgcaagatgc aacccaggac catgcagtgc tggaggccga gaggcagaag atgtcagccc
     1801 ttgtgcgagg gctgcagagg gagctggagg agacttcaga ggagacaggg cattggcaga
     1861 gtatgttcca gaagaacaag gaggatctta gagccaccaa gcaggaactc ctgcagctgc
     1921 gaatggagaa ggaggagatg gaagaggagc ttggagagaa gatagaggtc ttgcagaggg
     1981 aattagagca ggcccgagct agtgctggag atactcgcca ggttgaggtg ctcaagaagg
     2041 agctgctccg gacacaggag gagcttaagg aactgcaggc agaacggcag agccaggagg
     2101 tggctgggcg acaccgggac cgggagttgg agaagcagct ggcggtcctg agggtcgagg
     2161 ctgatcgagg tcgggagctg gaagaacaga acctccagct acaaaagacc ctccagcaac
     2221 tgcgacagga ctgtgaagag gcttccaagg ctaagatggt ggccgaggca gaggcaacag
     2281 tgctggggca gcggcgggcc gcagtggaga cgacgcttcg ggagacccag gaggaaaatg
     2341 acgaattccg ccggcgcatc ctgggtttgg agcagcagct gaaggagact cgaggtctgg
     2401 tggatggtgg ggaagcggtg gaggcacgac tacgggacaa gctgcagcgg ctggaggcag
     2461 agaaacagca gctggaggag gccctgaatg cgtcccagga agaggagggg agtctggcag
     2521 cagccaagcg ggcactggag gcacgcctag aggaggctca gcgggggctg gcccgcctgg
     2581 ggcaggagca gcagacactg aaccgggccc tggaggagga agggaagcag cgggaggtgc
     2641 tccggcgagg caaggctgag ctggaggagc agaagcgttt gctggacagg actgtggacc
     2701 gactgaacaa ggagttggag aagatcgggg aggactctaa gcaagccctg cagcagctcc
     2761 aggcccagct ggaggattat aaggaaaagg cccggcggga ggtggcagat gcccagcgcc
     2821 aggccaagga ttgggccagt gaggctgaga agacctctgg aggactgagc cgacttcagg
     2881 atgagatcca gaggctgcgg caggccctgc aggcatccca ggctgagcgg gacacagccc
     2941 ggctggacaa agagctactg gcccagcgac tgcaggggct ggagcaagag gcagagaaca
     3001 agaagcgttc ccaggacgac agggcccggc agctgaaggg tctcgaggaa aaagtctcac
     3061 ggctggaaac agagttagat gaggagaaga acaccgtgga gctgctaaca gatcgggtga
     3121 atcgtggccg ggaccaggtg gatcagctga ggacagagct catgcaggaa aggtctgctc
     3181 ggcaggacct ggagtgtgac aaaatctcct tggagagaca gaacaaggac ctgaagaccc
     3241 ggttggccag ctcagaaggc ttccagaagc ctagtgccag cctctctcag cttgagtccc
     3301 agaatcagtt gttgcaggag cggctacagg ctgaagagag ggagaagaca gttctgcagt
     3361 ctaccaatcg aaaactggag cggaaagtta aagaactatc catccagatt gaagacgagc
     3421 ggcagcatgt caatgaccag aaagaccagc taagcctgag ggtgaaggct ttgaagcgtc
     3481 aggtggatga agcagaagag gaaattgagc gactggacgg cctgaggaag aaggcccagc
     3541 gtgaggtgga ggagcagcat gaggtcaatg aacagctcca ggcccggatc aagtctctgg
     3601 agaaggactc ctggcgcaaa gcttcccgct cagctgctga gtcagctctc aaaaacgaag
     3661 ggctgagctc agatgaggaa ttcgacagtg tctacgatcc ctcgtccatt gcatcactgc
     3721 ttacggagag caacctacag accagctcct gttagctcgt ggtcctcaag gactcagaaa
     3781 ccaggctcga ggcctatccc agcaagtgct gctctgctct gcccaccctg ggttctgcat
     3841 tcctatgggt gacccaatta ttcagaccta agacagggag gggtcagagt gatggtgata
     3901 aaaaaaaaaa atcatcagca ataagctgat agatggactt tccactgtag gagtggacat
     3961 ttcaagccaa ctgagccttt tcctcaagtg ccgacacctc cctcatctct cttatagtgg
     4021 aaggatggtc agcattaggc tgatggggac tgagaaggat aggaagggat agaaattgcc
     4081 atgtgtataa agctttattc tttagccctt aaccctaagg ctcagggaaa taccctatgt
     4141 tattgtgctc cctggattcc tgcaactcat tttccttcca ctctggagca gggtgagggg
     4201 aatgttatgg gtaacagaca tgcaggcatg gctctaccca tttctttgca caagtatggg
     4261 gcccatgtgg tagtccccat acccctccag ttcctatatt tttgtcttct tcctttcccc
     4321 tctttgccat tcctaccttg catttttcct gtcagtgcct tagccaaggc aaggagataa
     4381 ggatgctctt cttgcttttt atatctgcac attcatacct ctccaaagac cagcttttcc
     4441 ccagccaggg ccctcagcct tccctgctgc cccagtgatt gattgagaga gctgttgggg
     4501 tttctctgcc aatgacccct gggagaggga ctttggtagg gtcatgataa agtggcgggg
     4561 gtctggtcct gctcagggtt ttcatccttc ctcctctccc tcctctgtga ctgtggatat
     4621 ggttataagg tggttgcacc tgggagccct gacaactggc tgcacaaatt ccaaaagtaa
     4681 aggtgtcagt ccctgtggcc ttccttgggg cttctctgac cacatgtgcc caacttcaat
     4741 aagagaacca agggaccctc attttctgag gtgcttggct ctgattcagg gctttgcaag
     4801 gggttagaag ctgactgtaa aaatgggaag aggcaacgga agacatttat ttctcctttg
     4861 gattttgggg agaaccaagc cctggtaggg aagaggtaag ggggatgatt cacctccata
     4921 tttcctaagc aggttgtata gggagccggt ggcaggagga aggctgtttt cacaaatgac
     4981 ttgtaatgtc gtgattaaaa aaattcctat attcttctgc aaatcaaacg ttctttccca
     5041 atccaatcca gccttggttt tattttaaat taaatattaa aattacacat ttatattgaa
     5101 a
//
