LOCUS       NM_000492               6070 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens CF transmembrane conductance regulator (CFTR), mRNA.
ACCESSION   NM_000492
VERSION     NM_000492.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Hong,J.S., Tindall,J.M., Tindall,S.R. and Sorscher,E.J.
  TITLE     Mutation accumulation in H. sapiens F508del CFTR countermands dN/dS
            type genomic analysis
  JOURNAL   PLoS One 19 (7), e0305832 (2024)
   PUBMED   39024311
  REMARK    GeneRIF: Mutation accumulation in H. sapiens F508del CFTR
            countermands dN/dS type genomic analysis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6070)
  AUTHORS   Merigo,F., Lagni,A., Boschi,F., Bernardi,P., Conti,A., Plebani,R.,
            Romano,M., Sorio,C., Lotti,V. and Sbarbati,A.
  TITLE     Loss of CFTR Reverses Senescence Hallmarks in SARS-CoV-2 Infected
            Bronchial Epithelial Cells
  JOURNAL   Int J Mol Sci 25 (11), 6185 (2024)
   PUBMED   38892373
  REMARK    GeneRIF: Loss of CFTR Reverses Senescence Hallmarks in SARS-CoV-2
            Infected Bronchial Epithelial Cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6070)
  AUTHORS   Al Abdulsalam,E.A.
  TITLE     Insights and considerations on CFTR variant reporting in a study of
            cystic fibrosis patients in Saudi Arabia CFTR 1548del G and 1549del
            G: Navigating the discovery of novel mutations
  JOURNAL   Saudi Med J 45 (5), 541 (2024)
   PUBMED   38734430
  REMARK    GeneRIF: Insights and considerations on CFTR variant reporting in a
            study of cystic fibrosis patients in Saudi Arabia CFTR 1548del G
            and 1549del G: Navigating the discovery of novel mutations.
REFERENCE   5  (bases 1 to 6070)
  AUTHORS   Shelton,C., LaRusch,J. and Whitcomb,D.C.
  TITLE     Pancreatitis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24624459
REFERENCE   6  (bases 1 to 6070)
  AUTHORS   Savant,A., Lyman,B., Bojanowski,C. and Upadia,J.
  TITLE     Cystic Fibrosis
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301428
REFERENCE   7  (bases 1 to 6070)
  AUTHORS   Lissens,W., Bonduelle,M., Malfroot,A., Dab,I. and Liebaers,I.
  TITLE     A serine to proline substitution (S1255P) in the second nucleotide
            binding fold of the cystic fibrosis gene
  JOURNAL   Hum Mol Genet 1 (6), 441-442 (1992)
   PUBMED   1284530
REFERENCE   8  (bases 1 to 6070)
  AUTHORS   Shackleton,S., Beards,F. and Harris,A.
  TITLE     Detection of novel and rare mutations in exon 4 of the cystic
            fibrosis gene by SSCP
  JOURNAL   Hum Mol Genet 1 (6), 439-440 (1992)
   PUBMED   1284529
REFERENCE   9  (bases 1 to 6070)
  AUTHORS   Cheadle,J.P., Meredith,A.L. and al-Jader,L.N.
  TITLE     A new missense mutation (R1283M) in exon 20 of the cystic fibrosis
            transmembrane conductance regulator gene
  JOURNAL   Hum Mol Genet 1 (2), 123-125 (1992)
   PUBMED   1284468
REFERENCE   10 (bases 1 to 6070)
  AUTHORS   Jones,C.T., McIntosh,I., Keston,M., Ferguson,A. and Brock,D.J.
  TITLE     Three novel mutations in the cystic fibrosis gene detected by
            chemical cleavage: analysis of variant splicing and a nonsense
            mutation
  JOURNAL   Hum Mol Genet 1 (1), 11-17 (1992)
   PUBMED   1284466
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M28668.1, AC000111.1 and
            AC000061.1.
            
            On Aug 31, 2019 this sequence version replaced NM_000492.3.
            
            Summary: This gene encodes a member of the ATP-binding cassette
            (ABC) transporter superfamily. The encoded protein functions as a
            chloride channel, making it unique among members of this protein
            family, and controls ion and water secretion and absorption in
            epithelial tissues. Channel activation is mediated by cycles of
            regulatory domain phosphorylation, ATP-binding by the
            nucleotide-binding domains, and ATP hydrolysis. Mutations in this
            gene cause cystic fibrosis, the most common lethal genetic disorder
            in populations of Northern European descent. The most frequently
            occurring mutation in cystic fibrosis, DeltaF508, results in
            impaired folding and trafficking of the encoded protein. Multiple
            pseudogenes have been identified in the human genome. [provided by
            RefSeq, Aug 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M28668.1, SRR18074969.707190.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000003084.11/ ENSP00000003084.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1477              M28668.1           63-1539
            1478-1496           AC000111.1         99186-99204
            1497-1910           M28668.1           1559-1972
            1911-1928           AC000111.1         131715-131732
            1929-2566           M28668.1           1991-2628
            2567-2588           AC000111.1         134643-134664
            2589-5669           M28668.1           2651-5731
            5670-6070           AC000061.1         58618-59018
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.2"
     gene            1..6070
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /note="CF transmembrane conductance regulator"
                     /db_xref="GeneID:1080"
                     /db_xref="HGNC:HGNC:1884"
                     /db_xref="MIM:602421"
     exon            1..123
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..4513
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /EC_number="5.6.1.6"
                     /note="cAMP-dependent chloride channel; channel
                     conductance-controlling ATPase; cystic fibrosis
                     transmembrane conductance regulator (ATP-binding cassette
                     sub-family C, member 7); cystic fibrosis transmembrane
                     conductance regulating"
                     /codon_start=1
                     /product="cystic fibrosis transmembrane conductance
                     regulator"
                     /protein_id="NP_000483.3"
                     /db_xref="CCDS:CCDS5773.1"
                     /db_xref="GeneID:1080"
                     /db_xref="HGNC:HGNC:1884"
                     /db_xref="MIM:602421"
                     /translation="MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVD
                     SADNLSEKLEREWDRELASKKNPKLINALRRCFFWRFMFYGIFLYLGEVTKAVQPLLL
                     GRIIASYDPDNKEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLI
                     YKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWEL
                     LQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRAGKISERLVITSEMIENIQSVKAYC
                     WEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIILR
                     KIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEV
                     VMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIER
                     GQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIF
                     GVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDA
                     DLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSS
                     YFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTET
                     KKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVP
                     DSEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAP
                     QANLTELDIYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITVH
                     KSLIFVLIWCLVIFLAEVAASLVVLWLLGNTPLQDKGNSTHSRNNSYAVIITSTSSYY
                     VFYIYVGVADTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGI
                     LNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLR
                     AYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTAN
                     WFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWA
                     VNSSIDVDSLMRSVSRVFKFIDMPTEGKPTKSTKPYKNGQLSKVMIIENSHVKKDDIW
                     PSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLN
                     TEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVAD
                     EVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDP
                     VTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLNERSL
                     FRQAISPSDRVKLFPHRNSSKCKSKPQIAALKEETEEEVQDTRL"
     exon            124..234
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            235..343
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            344..559
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            560..649
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            650..813
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            814..939
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1186
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1279
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1462
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1654
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1749
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1836
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..2560
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2689
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..2727
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2978
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            2979..3058
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            3059..3209
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            3210..3437
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            3438..3538
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3787
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            3788..3943
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            3944..4033
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            4034..4206
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            4207..4312
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     exon            4313..6070
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6045..6050
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /note="hexamer: AATAAA"
     polyA_site      6070
                     /gene="CFTR"
                     /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1;
                     MRP7; TNR-CFTR"
                     /note="major polyA site"
ORIGIN      
        1 gtagtaggtc tttggcatta ggagcttgag cccagacggc cctagcaggg accccagcgc
       61 ccgagagacc atgcagaggt cgcctctgga aaaggccagc gttgtctcca aacttttttt
      121 cagctggacc agaccaattt tgaggaaagg atacagacag cgcctggaat tgtcagacat
      181 ataccaaatc ccttctgttg attctgctga caatctatct gaaaaattgg aaagagaatg
      241 ggatagagag ctggcttcaa agaaaaatcc taaactcatt aatgcccttc ggcgatgttt
      301 tttctggaga tttatgttct atggaatctt tttatattta ggggaagtca ccaaagcagt
      361 acagcctctc ttactgggaa gaatcatagc ttcctatgac ccggataaca aggaggaacg
      421 ctctatcgcg atttatctag gcataggctt atgccttctc tttattgtga ggacactgct
      481 cctacaccca gccatttttg gccttcatca cattggaatg cagatgagaa tagctatgtt
      541 tagtttgatt tataagaaga ctttaaagct gtcaagccgt gttctagata aaataagtat
      601 tggacaactt gttagtctcc tttccaacaa cctgaacaaa tttgatgaag gacttgcatt
      661 ggcacatttc gtgtggatcg ctcctttgca agtggcactc ctcatggggc taatctggga
      721 gttgttacag gcgtctgcct tctgtggact tggtttcctg atagtccttg ccctttttca
      781 ggctgggcta gggagaatga tgatgaagta cagagatcag agagctggga agatcagtga
      841 aagacttgtg attacctcag aaatgattga aaatatccaa tctgttaagg catactgctg
      901 ggaagaagca atggaaaaaa tgattgaaaa cttaagacaa acagaactga aactgactcg
      961 gaaggcagcc tatgtgagat acttcaatag ctcagccttc ttcttctcag ggttctttgt
     1021 ggtgttttta tctgtgcttc cctatgcact aatcaaagga atcatcctcc ggaaaatatt
     1081 caccaccatc tcattctgca ttgttctgcg catggcggtc actcggcaat ttccctgggc
     1141 tgtacaaaca tggtatgact ctcttggagc aataaacaaa atacaggatt tcttacaaaa
     1201 gcaagaatat aagacattgg aatataactt aacgactaca gaagtagtga tggagaatgt
     1261 aacagccttc tgggaggagg gatttgggga attatttgag aaagcaaaac aaaacaataa
     1321 caatagaaaa acttctaatg gtgatgacag cctcttcttc agtaatttct cacttcttgg
     1381 tactcctgtc ctgaaagata ttaatttcaa gatagaaaga ggacagttgt tggcggttgc
     1441 tggatccact ggagcaggca agacttcact tctaatggtg attatgggag aactggagcc
     1501 ttcagagggt aaaattaagc acagtggaag aatttcattc tgttctcagt tttcctggat
     1561 tatgcctggc accattaaag aaaatatcat ctttggtgtt tcctatgatg aatatagata
     1621 cagaagcgtc atcaaagcat gccaactaga agaggacatc tccaagtttg cagagaaaga
     1681 caatatagtt cttggagaag gtggaatcac actgagtgga ggtcaacgag caagaatttc
     1741 tttagcaaga gcagtataca aagatgctga tttgtattta ttagactctc cttttggata
     1801 cctagatgtt ttaacagaaa aagaaatatt tgaaagctgt gtctgtaaac tgatggctaa
     1861 caaaactagg attttggtca cttctaaaat ggaacattta aagaaagctg acaaaatatt
     1921 aattttgcat gaaggtagca gctattttta tgggacattt tcagaactcc aaaatctaca
     1981 gccagacttt agctcaaaac tcatgggatg tgattctttc gaccaattta gtgcagaaag
     2041 aagaaattca atcctaactg agaccttaca ccgtttctca ttagaaggag atgctcctgt
     2101 ctcctggaca gaaacaaaaa aacaatcttt taaacagact ggagagtttg gggaaaaaag
     2161 gaagaattct attctcaatc caatcaactc tatacgaaaa ttttccattg tgcaaaagac
     2221 tcccttacaa atgaatggca tcgaagagga ttctgatgag cctttagaga gaaggctgtc
     2281 cttagtacca gattctgagc agggagaggc gatactgcct cgcatcagcg tgatcagcac
     2341 tggccccacg cttcaggcac gaaggaggca gtctgtcctg aacctgatga cacactcagt
     2401 taaccaaggt cagaacattc accgaaagac aacagcatcc acacgaaaag tgtcactggc
     2461 ccctcaggca aacttgactg aactggatat atattcaaga aggttatctc aagaaactgg
     2521 cttggaaata agtgaagaaa ttaacgaaga agacttaaag gagtgctttt ttgatgatat
     2581 ggagagcata ccagcagtga ctacatggaa cacatacctt cgatatatta ctgtccacaa
     2641 gagcttaatt tttgtgctaa tttggtgctt agtaattttt ctggcagagg tggctgcttc
     2701 tttggttgtg ctgtggctcc ttggaaacac tcctcttcaa gacaaaggga atagtactca
     2761 tagtagaaat aacagctatg cagtgattat caccagcacc agttcgtatt atgtgtttta
     2821 catttacgtg ggagtagccg acactttgct tgctatggga ttcttcagag gtctaccact
     2881 ggtgcatact ctaatcacag tgtcgaaaat tttacaccac aaaatgttac attctgttct
     2941 tcaagcacct atgtcaaccc tcaacacgtt gaaagcaggt gggattctta atagattctc
     3001 caaagatata gcaattttgg atgaccttct gcctcttacc atatttgact tcatccagtt
     3061 gttattaatt gtgattggag ctatagcagt tgtcgcagtt ttacaaccct acatctttgt
     3121 tgcaacagtg ccagtgatag tggcttttat tatgttgaga gcatatttcc tccaaacctc
     3181 acagcaactc aaacaactgg aatctgaagg caggagtcca attttcactc atcttgttac
     3241 aagcttaaaa ggactatgga cacttcgtgc cttcggacgg cagccttact ttgaaactct
     3301 gttccacaaa gctctgaatt tacatactgc caactggttc ttgtacctgt caacactgcg
     3361 ctggttccaa atgagaatag aaatgatttt tgtcatcttc ttcattgctg ttaccttcat
     3421 ttccatttta acaacaggag aaggagaagg aagagttggt attatcctga ctttagccat
     3481 gaatatcatg agtacattgc agtgggctgt aaactccagc atagatgtgg atagcttgat
     3541 gcgatctgtg agccgagtct ttaagttcat tgacatgcca acagaaggta aacctaccaa
     3601 gtcaaccaaa ccatacaaga atggccaact ctcgaaagtt atgattattg agaattcaca
     3661 cgtgaagaaa gatgacatct ggccctcagg gggccaaatg actgtcaaag atctcacagc
     3721 aaaatacaca gaaggtggaa atgccatatt agagaacatt tccttctcaa taagtcctgg
     3781 ccagagggtg ggcctcttgg gaagaactgg atcagggaag agtactttgt tatcagcttt
     3841 tttgagacta ctgaacactg aaggagaaat ccagatcgat ggtgtgtctt gggattcaat
     3901 aactttgcaa cagtggagga aagcctttgg agtgatacca cagaaagtat ttattttttc
     3961 tggaacattt agaaaaaact tggatcccta tgaacagtgg agtgatcaag aaatatggaa
     4021 agttgcagat gaggttgggc tcagatctgt gatagaacag tttcctggga agcttgactt
     4081 tgtccttgtg gatgggggct gtgtcctaag ccatggccac aagcagttga tgtgcttggc
     4141 tagatctgtt ctcagtaagg cgaagatctt gctgcttgat gaacccagtg ctcatttgga
     4201 tccagtaaca taccaaataa ttagaagaac tctaaaacaa gcatttgctg attgcacagt
     4261 aattctctgt gaacacagga tagaagcaat gctggaatgc caacaatttt tggtcataga
     4321 agagaacaaa gtgcggcagt acgattccat ccagaaactg ctgaacgaga ggagcctctt
     4381 ccggcaagcc atcagcccct ccgacagggt gaagctcttt ccccaccgga actcaagcaa
     4441 gtgcaagtct aagccccaga ttgctgctct gaaagaggag acagaagaag aggtgcaaga
     4501 tacaaggctt tagagagcag cataaatgtt gacatgggac atttgctcat ggaattggag
     4561 ctcgtgggac agtcacctca tggaattgga gctcgtggaa cagttacctc tgcctcagaa
     4621 aacaaggatg aattaagttt ttttttaaaa aagaaacatt tggtaagggg aattgaggac
     4681 actgatatgg gtcttgataa atggcttcct ggcaatagtc aaattgtgtg aaaggtactt
     4741 caaatccttg aagatttacc acttgtgttt tgcaagccag attttcctga aaacccttgc
     4801 catgtgctag taattggaaa ggcagctcta aatgtcaatc agcctagttg atcagcttat
     4861 tgtctagtga aactcgttaa tttgtagtgt tggagaagaa ctgaaatcat acttcttagg
     4921 gttatgatta agtaatgata actggaaact tcagcggttt atataagctt gtattccttt
     4981 ttctctcctc tccccatgat gtttagaaac acaactatat tgtttgctaa gcattccaac
     5041 tatctcattt ccaagcaagt attagaatac cacaggaacc acaagactgc acatcaaaat
     5101 atgccccatt caacatctag tgagcagtca ggaaagagaa cttccagatc ctggaaatca
     5161 gggttagtat tgtccaggtc taccaaaaat ctcaatattt cagataatca caatacatcc
     5221 cttacctggg aaagggctgt tataatcttt cacaggggac aggatggttc ccttgatgaa
     5281 gaagttgata tgccttttcc caactccaga aagtgacaag ctcacagacc tttgaactag
     5341 agtttagctg gaaaagtatg ttagtgcaaa ttgtcacagg acagcccttc tttccacaga
     5401 agctccaggt agagggtgtg taagtagata ggccatgggc actgtgggta gacacacatg
     5461 aagtccaagc atttagatgt ataggttgat ggtggtatgt tttcaggcta gatgtatgta
     5521 cttcatgctg tctacactaa gagagaatga gagacacact gaagaagcac caatcatgaa
     5581 ttagttttat atgcttctgt tttataattt tgtgaagcaa aattttttct ctaggaaata
     5641 tttattttaa taatgtttca aacatatata acaatgctgt attttaaaag aatgattatg
     5701 aattacattt gtataaaata atttttatat ttgaaatatt gactttttat ggcactagta
     5761 tttctatgaa atattatgtt aaaactggga caggggagaa cctagggtga tattaaccag
     5821 gggccatgaa tcaccttttg gtctggaggg aagccttggg gctgatgcag ttgttgccca
     5881 cagctgtatg attcccagcc agcacagcct cttagatgca gttctgaaga agatggtacc
     5941 accagtctga ctgtttccat caagggtaca ctgccttctc aactccaaac tgactcttaa
     6001 gaagactgca ttatatttat tactgtaaga aaatatcact tgtcaataaa atccatacat
     6061 ttgtgtgaaa
//
