LOCUS       NM_001164496           10238 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens cilia and flagella associated protein 44 (CFAP44),
            transcript variant 1, mRNA.
ACCESSION   NM_001164496
VERSION     NM_001164496.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   Wu,H., Li,W., He,X., Liu,C., Fang,Y., Zhu,F., Jiang,H., Liu,W.,
            Song,B., Wang,X., Zhou,P., Wei,Z., Zhang,F. and Cao,Y.
  TITLE     NovelCFAP43 andCFAP44 mutations cause male infertility with
            multiple morphological abnormalities of the sperm flagella (MMAF)
  JOURNAL   Reprod Biomed Online 38 (5), 769-778 (2019)
   PUBMED   30904354
  REMARK    GeneRIF: important cause of multiple morphological abnormalities of
            the sperm flagellum in the Chinese population
REFERENCE   3  (bases 1 to 10238)
  AUTHORS   Sha,Y.W., Wang,X., Xu,X., Su,Z.Y., Cui,Y., Mei,L.B., Huang,X.J.,
            Chen,J., He,X.M., Ji,Z.Y., Bao,H., Yang,X., Li,P. and Li,L.
  TITLE     Novel Mutations in CFAP44 and CFAP43 Cause Multiple Morphological
            Abnormalities of the Sperm Flagella (MMAF)
  JOURNAL   Reprod Sci 26 (1), 26-34 (2019)
   PUBMED   29277146
  REMARK    GeneRIF: Study demonstrated that biallelic mutations in CFAP44 and
            CFAP43 cause multiple morphological abnormalities of the sperm
            flagella (MMAF). These results provide researchers with a new
            insight to understand the genetic etiology of MMAF and to identify
            new loci for genetic counselling of MMAF.
REFERENCE   4  (bases 1 to 10238)
  AUTHORS   Coutton,C., Vargas,A.S., Amiri-Yekta,A., Kherraf,Z.E., Ben
            Mustapha,S.F., Le Tanno,P., Wambergue-Legrand,C., Karaouzene,T.,
            Martinez,G., Crouzy,S., Daneshipour,A., Hosseini,S.H., Mitchell,V.,
            Halouani,L., Marrakchi,O., Makni,M., Latrous,H., Kharouf,M.,
            Deleuze,J.F., Boland,A., Hennebicq,S., Satre,V., Jouk,P.S.,
            Thierry-Mieg,N., Conne,B., Dacheux,D., Landrein,N., Schmitt,A.,
            Stouvenel,L., Lores,P., El Khouri,E., Bottari,S.P., Faure,J.,
            Wolf,J.P., Pernet-Gallay,K., Escoffier,J., Gourabi,H.,
            Robinson,D.R., Nef,S., Dulioust,E., Zouari,R., Bonhivers,M.,
            Toure,A., Arnoult,C. and Ray,P.F.
  TITLE     Mutations in CFAP43 and CFAP44 cause male infertility and flagellum
            defects in Trypanosoma and human
  JOURNAL   Nat Commun 9 (1), 686 (2018)
   PUBMED   29449551
  REMARK    GeneRIF: demonstrate that CFAP43 and CFAP44 have a similar
            structure with a unique axonemal localization and are necessary to
            produce functional flagella in species ranging from Trypanosoma to
            human
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10238)
  AUTHORS   Tanaka,A.S. and Tanaka,M.
  TITLE     A novel gene, cilia flagella associated protein 44, encoding an
            enzyme cleaving FtsZ and tubulin contributes to the regulation of
            secretory pathway
  JOURNAL   Biochem Biophys Res Commun 493 (1), 399-408 (2017)
   PUBMED   28887034
REFERENCE   6  (bases 1 to 10238)
  AUTHORS   Tang,S., Wang,X., Li,W., Yang,X., Li,Z., Liu,W., Li,C., Zhu,Z.,
            Wang,L., Wang,J., Zhang,L., Sun,X., Zhi,E., Wang,H., Li,H., Jin,L.,
            Luo,Y., Wang,J., Yang,S. and Zhang,F.
  TITLE     Biallelic Mutations in CFAP43 and CFAP44 Cause Male Infertility
            with Multiple Morphological Abnormalities of the Sperm Flagella
  JOURNAL   Am J Hum Genet 100 (6), 854-864 (2017)
   PUBMED   28552195
  REMARK    GeneRIF: biallelic mutations in either CFAP43 or CFAP44 can cause
            sperm flagellar abnormalities and impair sperm motility.
REFERENCE   7  (bases 1 to 10238)
  AUTHORS   de Keyser,C.E., Becker,M.L., Hofman,A., Lous,J.J.,
            Uitterlinden,A.G., Visser,L.E. and Stricker,B.H.
  TITLE     The rs13064411 polymorphism in the WDR52 gene, associated with
            PCSK9 levels, modifies statin-induced changes in serum total and
            LDL cholesterol levels
  JOURNAL   Pharmacogenet Genomics 25 (3), 134-142 (2015)
   PUBMED   25602530
  REMARK    GeneRIF: Results show that the rs13064411 polymorphism in the WDR52
            gene was associated with a modified effect of statin therapy on
            serum LDL and total cholesterol levels.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB050626.1, BC113553.1, AL133606.1, AK002004.1, AC026329.14 and
            BM978031.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001164496.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393845.9/ ENSP00000377428.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DB050626.1         22-41
            21-2637             BC113553.1         1-2617
            2638-4913           AL133606.1         1-2276
            4914-5015           AK002004.1         730-831
            5016-5016           AC026329.14        16117-16117
            5017-5196           AK002004.1         833-1012
            5197-5548           AL133606.1         2560-2911
            5549-9637           AC026329.14        28521-32609
            9638-10238          BM978031.1         19-619              c
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.2"
     gene            1..10238
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="cilia and flagella associated protein 44"
                     /db_xref="GeneID:55779"
                     /db_xref="HGNC:HGNC:25631"
                     /db_xref="MIM:617559"
     exon            1..41
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            42..146
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..5611
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="isoform 1 is encoded by transcript variant 1; WD
                     repeat-containing protein 52; WD repeat domain 52"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 44
                     isoform 1"
                     /protein_id="NP_001157968.1"
                     /db_xref="CCDS:CCDS54624.1"
                     /db_xref="GeneID:55779"
                     /db_xref="HGNC:HGNC:25631"
                     /db_xref="MIM:617559"
                     /translation="MKEPDDQDTDGEKSVTSKSDGKKSLRSSKSESRSPVQEDNTFLE
                     DDTDETFTKGEGSYLEEDSDEERLEGSLSSFQYGDLQSTTVPQQTPAPAVEEAEEEVK
                     KKISESFFYDYMELASMPFVTLDSNIPLDLLTLVHSFGYDCRKRANLQLLDDSIAIYI
                     AGNQLIFLNLKTKEQIYLRSSSGEGIGVIGVHPHKTYFTVAEKGSFPDIIIYEYPSLR
                     PYRVLRDGTEKGYAYVDFNYSGNLLASVGSNPDYTLTIWNWKEEQPILRTKAFSQEVF
                     KVTFNPDKEEQLTTSGSGHIKFWEMAFTFTGLKLQGSLGRFGKTITTDIEGYMELPDG
                     KVLSGSEWGNMLLWEGGLIKVELCRGTSKSCHNGPINQIMLYEGEVITVGSDGYVRIW
                     DFETIDTADVIDETGLLEIEPINELQVDKNVNLFSMIKMNETGNNFWLAQDANGAIWK
                     LDLSFSNITQDPECLFSFHSGAIEAVAVSPLTYLMATTALDCSVRIYDFASKTPLAQM
                     KFKQGGTALVWVPRMVNFTGAQIIVGFEDGVVRILELYDPKGLTIFAGRKKILDADIQ
                     LKQVFKPHTACVTALAYERDGEILATGSKDQTVFFFEVERDYKPIGYINTPGPVCQLM
                     WSPMSHPESTLLIICENGYILEAPLPTIKQEEDDHDVVSYEIKDMCIKCFHFSSVKSK
                     ILRLIEIEKRERQRELKEKIREERRNKLAAEMGEDGEKEFQEEEEEKEEEEEEEEPLP
                     EIFIPSTPSPILCGFYSEPGKFWVSLGGYDSGFLYHCEFPPCDESSDFKEQKDEPIDV
                     RYLADTEDNPIQTITFNINKVMMFCGMKNGAIRVYVLNQNDPSLTSLVDYWHFNMHDN
                     NYGCIKSIANSFDDRFLVTAGADGNIFVFNIFSEFMLRKDMKAKVPSPRFGIETEPIP
                     EDIEDPKAYSIENARRKREHDKLMKEVGEIKARKREQIKALRSEFCNLLEMNEKLPKH
                     MQFKRTDFDVDSQIRAEMHRKTAFKIQQVEKELAWEKEKHELGLMKLKNRFRDPLESD
                     TIVVHAILSDHKISSYRLVQPSKYSKFKRASQSERKPSKLDRFEKEGPGRKDSQRDAG
                     GSVTIQEESIIEKGKKFRPKTLSEIIVENQIEKTRKLILKAERAQLKIQQRKKEWEEL
                     YKSKPGDDYEDPKDLQAIKEAQVYMGDFNLKTAPDYKIPEHMRINAAKKEEELGHLDS
                     LVHGNKRHMNKCILSLRDLKVAVVEEIQCLVQELKNIQSTLHISKHIPIPKIPQIHPE
                     EVPEKRFQYDEETLLNFKQQQMKSKDEKSPGVEQTGSGGPVGGFLKLSSRKDGDLTTR
                     DSISRSSKASTFSLDIPKCLEFEKAEPTDVELEIMKRDEIKHVYMQQYLVNRIKELVV
                     TFDAELRLLRHQKLKLDTQMKLSDLHHVTLFQEILLLKNFEKQENILQERVNSLDKEE
                     QYMQWKINETLKEMEEKKNEITKLQEQEKALYAGFQAAIGENNKFANFLMKVLKKKIK
                     RVKKKEVEGDADEDEESEESSEEESSLESDEDESESEDEVFDDSICPTNCDVALFELA
                     LHLREKRLDIEEALVEEKKIVDNLKKEYDTLSKKVKIVATNLNAAEEALEAYQREKQQ
                     RLNELLVVIPLKLHQIEYVVFGEIPSDLSGTLVFSNHALRRLQERIHELQEENSKQQK
                     LNKEWRERRKQLIREKREMTKTIHKMEETVRQLMISKFGRVVNLEALQTLSVNTTLEE
                     LKIRKLRKELANAKEMKMWEEKIAQMRWELMMKTKEHTRKLYQMNDLCIEKKKLDSRL
                     NTLQNQQGNAFQGPREADVVAREEVTELIQLQAERISALKEEIALLRRKGSLILPPIQ
                     SPREKEIQPADL"
     exon            147..299
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            300..453
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            454..616
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            617..719
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            720..936
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1051
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1216
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1372
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1420
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1520
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1615
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1825
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1936
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2098
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2344
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2490
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2761
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2817
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..2980
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..3111
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            3112..3308
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            3309..3483
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            3484..3661
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            3662..4162
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            4163..4366
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            4367..4562
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            4563..4673
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            4674..4804
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            4805..4921
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            4922..5123
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            5124..5284
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            5285..5419
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     exon            5420..10238
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6307..6312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="hexamer: AATAAA"
     polyA_site      6328
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="major polyA site"
     polyA_site      7050
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="major polyA site"
     regulatory      10216..10221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="hexamer: AATAAA"
     polyA_site      10238
                     /gene="CFAP44"
                     /gene_synonym="SPGF20; WDR52"
                     /note="major polyA site"
ORIGIN      
        1 agacgctgtg aagagaggag ccgcggcggt cagtaccttc gaggaaatga aggaaccaga
       61 tgatcaggat actgatgggg agaaatcagt tacatcaaag agtgatggga agaagtctct
      121 gaggtcttct aaatcagaat caagatctcc tgttcaagaa gataacacat ttttagaaga
      181 tgacacagat gaaacattta ccaaagggga aggatcatat ttagaagaag actcagatga
      241 ggaacgtttg gaaggaagtt tgagttcatt tcagtatggt gatttgcaaa gcactacagt
      301 acctcagcaa acccctgctc cagctgtgga agaagcagag gaggaagtta agaagaaaat
      361 atcagagagc ttcttctatg attatatgga gcttgcttcg atgccttttg tgactctgga
      421 ttcaaacata ccactggatc ttctcacact tgtacattct tttggttatg actgtagaaa
      481 gcgagccaac ctacaacttc tggacgacag tatcgccata tacatagctg ggaaccaact
      541 gatctttctg aatttgaaaa ccaaggaaca gatctacctg cgaagtagca gtggtgaagg
      601 aattggcgtc attggggttc atccacataa aacttatttc acagtagctg aaaaagggag
      661 ttttccagat attatcatct atgaatatcc ttctctgaga ccatacagag tccttcgaga
      721 tgggactgag aagggatatg cttatgtgga ctttaactac agcggtaact tgctggcctc
      781 tgttggtagt aaccctgact acacactgac tatctggaac tggaaagaag aacaacccat
      841 actaaggaca aaagcttttt ctcaggaagt ttttaaggtt actttcaatc ctgataagga
      901 agagcagctt actacatcgg gatcaggcca catcaagttc tgggaaatgg cttttacgtt
      961 caccggtctc aagctgcagg gatcactagg tcgatttggc aaaacaatca ctactgatat
     1021 agaaggctac atggagctcc cagatgggaa ggtgctctca gggtcagaat ggggcaacat
     1081 gctgctttgg gaaggtggtc tgatcaaagt ggagctctgt cgagggacaa gcaagtcatg
     1141 tcacaatggt cccattaacc agataatgct gtatgagggt gaagttatca ctgttgggtc
     1201 agatggatat gttaggatat gggattttga gacaatagac actgctgatg taatagatga
     1261 gactggattg ttggagattg agcctattaa tgaacttcaa gtagacaaga atgtgaatct
     1321 cttctctatg ataaaaatga atgaaactgg aaataacttt tggttggctc aggatgccaa
     1381 tggagccata tggaagcttg accttagttt ttcaaatatt acccaggacc cagaatgcct
     1441 cttctccttc cattctggag ctattgaagc cgtggctgtt tctcctctca cttatctcat
     1501 ggccacaact gccttggact gctctgttcg aatctatgat tttgctagca aaactccttt
     1561 ggcccagatg aaattcaaac aaggaggtac tgcccttgtt tgggtacccc gaatggtaaa
     1621 cttcactgga gcacaaatta ttgtaggatt tgaagatgga gttgttcgaa ttcttgaact
     1681 ttatgatcca aaagggctca cgatttttgc gggacggaag aaaattttgg atgctgatat
     1741 tcagttgaaa caggttttca aaccccatac tgcttgtgtc actgctttag cttatgaacg
     1801 tgatggggaa attctagcca cagggagtaa agatcaaact gttttcttct ttgaagtgga
     1861 aagggattat aagccgattg gttatattaa tactcctgga cctgtgtgtc agttaatgtg
     1921 gtctcccatg tctcatcctg aaagtacttt actaattatc tgtgaaaatg gctatattct
     1981 tgaagctcca cttccaacca taaagcaaga agaggatgat catgatgtag tctcctatga
     2041 aatcaaagac atgtgcataa aatgtttcca tttttcaagt gtcaaatcta agattctgag
     2101 attaatagaa attgaaaaga gggagagaca aagggagttg aaggagaaaa taagggaaga
     2161 aaggaggaac aagctagcag cagagatggg agaagatgga gaaaaagaat ttcaggagga
     2221 ggaagaggag aaagaggagg aggaggagga agaagagcca ttacctgaaa tatttattcc
     2281 gtcaaccccc tctcccatcc tctgtggatt ttactcagag ccagggaagt tctgggtttc
     2341 tttgggtggc tatgattctg gttttctata tcactgtgag ttcccccctt gtgatgaaag
     2401 cagtgatttc aaagaacaaa aagatgaacc tattgatgtc cgttatcttg cggatacaga
     2461 ggacaatccc atccaaacta tcactttcaa cattaacaaa gttatgatgt tttgtggaat
     2521 gaaaaatgga gcaattcgag tctatgtcct aaatcaaaat gatccttcat tgaccagttt
     2581 ggtggactac tggcacttca atatgcatga caataattat ggatgtatta aaagtattgc
     2641 taatagcttt gatgatcgtt tcttggtgac tgctggagca gatggcaata tctttgtttt
     2701 caacattttt tctgaattta tgctaaggaa agacatgaag gccaaagttc catctcccag
     2761 gtttggaatt gaaacagagc caattccaga agacattgaa gatcccaaag cctacagtat
     2821 cgagaatgct aggagaaaaa gagaacatga caagttaatg aaagaagtgg gagaaataaa
     2881 ggcacggaag agagagcaaa tcaaagcttt gaggagtgaa ttttgtaatc tattagaaat
     2941 gaatgaaaaa ttaccaaagc atatgcagtt taaacgaaca gattttgatg tagattccca
     3001 aatccgtgct gagatgcaca gaaaaacagc tttcaaaatt caacaagtgg aaaaggaatt
     3061 agcttgggaa aaagagaaac atgaactcgg cctaatgaag ctaaagaatc gatttcgaga
     3121 tccactggaa agtgatacta ttgtggttca tgccatactg agtgaccaca agatatcctc
     3181 ttacaggctg gtgcagccct ctaagtactc caaattcaaa cgagctagtc aatcagagag
     3241 aaaaccaagc aaattggaca ggtttgaaaa agagggacct ggaagaaagg acagccagag
     3301 agatgcaggt gggagtgtta ccatacaaga agaatcaata atagaaaaag ggaagaaatt
     3361 tcggcctaaa accctaagtg aaatcatcgt ggaaaatcaa attgagaaaa cgagaaaact
     3421 tatattaaaa gctgaaaggg cacaactaaa gattcaacag cgaaaaaaag aatgggagga
     3481 actatacaag agtaaacctg gtgatgacta tgaagatccc aaagatcttc aagccatcaa
     3541 agaagcccag gtgtatatgg gagatttcaa tctgaagaca gccccagact acaagatacc
     3601 tgagcacatg agaataaatg ctgccaagaa ggaagaggaa ctaggacacc tagattcttt
     3661 ggtccatgga aataaaaggc acatgaacaa gtgcattctc tctcttagag accttaaagt
     3721 ggctgttgtt gaggaaatac agtgcctggt acaagaactg aagaacattc agtcgactct
     3781 tcacatatcc aagcacatac ccattcccaa aattcctcag atacacccag aagaagttcc
     3841 agaaaagaga tttcagtatg atgaagaaac tctcctaaat tttaagcaac agcaaatgaa
     3901 aagtaaagat gaaaagtccc ccggagtgga acagacaggg tctggaggcc cagttggagg
     3961 attcctcaaa ctctcttcta gaaaggatgg ggatttgaca acccgtgatt caatatctag
     4021 atcatcaaag gcatcaacat tcagcctaga tataccaaag tgtttggaat ttgaaaaagc
     4081 agagccaacg gatgtggagc tggaaattat gaagagagac gagattaaac acgtgtacat
     4141 gcaacagtat ttggtcaaca ggatcaaaga actggttgtc actttcgatg cagaactccg
     4201 tctccttaga catcagaaac taaaactaga tactcagatg aaattatctg acctgcacca
     4261 tgtcacctta tttcaagaaa tacttctcct gaagaatttt gaaaaacagg agaacatact
     4321 tcaagaacgt gttaattcct tagacaaaga ggaacagtac atgcaatgga aaataaatga
     4381 aactcttaaa gagatggaag agaaaaagaa tgaaatcacc aaactccagg agcaagaaaa
     4441 ggcactctat gctggttttc aagcagccat tggagaaaac aataaatttg caaacttcct
     4501 catgaaggtc ctaaagaaga agattaaacg ggtaaagaaa aaagaagttg aaggagatgc
     4561 tgatgaagat gaggagagtg aggaatcaag tgaagaagaa tctagcttgg agagtgatga
     4621 agatgagtct gaatcagaag atgaggtttt tgatgattct atttgcccaa caaattgtga
     4681 tgtggctctt tttgagctgg cccttcacct tcgagagaaa aggctggaca ttgaggaggc
     4741 tttagttgaa gaaaagaaaa ttgttgataa cctcaaaaag gaatatgata cattgtcaaa
     4801 aaaagtgaaa attgtggcaa ctaatctgaa tgcagcagag gaggccctgg aggcttatca
     4861 gcgagagaag cagcagcggc tgaatgaact gctagttgtg attccgctca agctccacca
     4921 gatagagtat gtggtatttg gagaaatacc tagcgatctt tctggtactt tggtcttctc
     4981 taaccatgcc ttgagacggc tgcaagaacg aatccatgag ctccaggagg aaaattccaa
     5041 gcagcaaaaa cttaacaaag aatggagaga gagacgtaaa cagctcatcc gagaaaagag
     5101 agaaatgaca aaaaccatac acaaaatgga ggaaacagtc cggcagctca tgatcagcaa
     5161 gtttggccgt gtggtaaatc tagaagccct tcaaacgctt tctgttaata caacacttga
     5221 agaactgaag atcagaaaac ttcgaaagga gctagcaaat gcgaaagaga tgaagatgtg
     5281 ggaggaaaag attgctcaaa tgcgatggga actgatgatg aagacaaaag aacacaccag
     5341 aaagctttat cagatgaatg atttgtgtat tgaaaagaag aaacttgatt ctcggttgaa
     5401 tacactacag aatcagcagg gcaatgcctt ccagggccct cgggaagcag atgttgtggc
     5461 aagagaggag gtcactgaat tgatccaact ccaggcggaa aggatttcgg ccttaaagga
     5521 ggagattgct cttttgcgta ggaaaggcag tcttatcctc ccacccattc agtctccacg
     5581 agagaaagag atacagcccg cagacctttg agtttgctat tttctcaaac ccatacaaga
     5641 tttctgacat aactcactgg aagttctgtc tcctcatcac ctccaacaat cttattactt
     5701 taaactgaac tcgcctgaaa gtgtaaaacc agatccagtc acgttaagtc actttaagaa
     5761 tctctctaca atttggttat cttagaactg actgcagcac ccaacctctg ttatgaaact
     5821 gaatcatttt agatgtttcg aacctactag attgaatatg aagtctcgga aggctgtgtt
     5881 tcatgattgc agcaaatcac ctgtaatata ttttgtttta ggaaaatttg atttgggggt
     5941 atcctgaatt tttagccctt ggcaagaaaa actatcattc agcaattcat gagaaaactt
     6001 tgttttcttg atccttagaa aataaactta gatgttatta agtgttctct gagaggatgc
     6061 aggagaaaag gacattttgt cagctatatc ttagaataga aatgcaaatt tcagttttgg
     6121 cattgttgac ttcataaagc agtgttttga gggctcagga ttcaggaaat acaaacagac
     6181 atggcagagc agtttgcttt gggttggcag aaagcaggaa aataaccagc cttcttggat
     6241 tgcccacagt ttttgtagac tcctcagttc tgagtggagc agaggggcat gcttaataaa
     6301 acagtcaata aagaggcttt tctttaaaga tttctgcaac tccgtcagaa ttctaagttt
     6361 tccccatagt ttctcatgtc ccctgccatt ttctttcagg ttttacttgg gccatttgct
     6421 ggttttgata tagatgagga gataatgaaa tagatttact agagatcatc tttaacaatc
     6481 acctttgatt atgcataaca ttttaaacta attgaagagt atccttttgg tttgggggta
     6541 agtgctcatt gaagttcatg gctataacca ttgcaggata atctcactga catttggaat
     6601 cctgttagta acagagcata gctctttaga ttaagagctc tgtattaacg agctactcat
     6661 tgaggctgtt ccaggataag ctggtcactg agaagggcgg gtgaagaaag gaacattgca
     6721 taaatgtaga agggaactca gcaatgccaa gtcttcctca cctgtgatta gcctgctaat
     6781 gtgcccccca aaattacaga caggtcttgc tttctttgta catcagcata ttcttagagg
     6841 tggccacttg gcctattgtc ttcctaagat tccttcatat tcctatttaa gaggtcagaa
     6901 taccttattg tttgcacttg tcaccattaa tttctgatgt agtcacttct ctactggaga
     6961 gcacccactt ttttttttaa atcactttct gtgttgtata tcctaagcca tgtgtgcctt
     7021 tcaagtattt ttaaaaatat gctagtcata gccctgagtg tcttatttat tgcggtaaaa
     7081 aattaccccc aaacttagct tgaaacaaca aacttgtgat ctcacccagg agtgagaggg
     7141 tttctgaggt ccaggaatct ggggacagct tggccgggtg ttctggctca gggtctttcg
     7201 tggagttgca gtcacactag gagctggggc tgcagccatc tgaagcttgg ctggagctgg
     7261 aggggctatt agcaaattca ctcccttggc cattaggcag ggcttcagtt cctcgccatg
     7321 tgggcctctc catagggccg cttgggatat gacttctccc agagcaagtg atccaagagt
     7381 gagggagaca gcaagagaga atgatgtata caagagccac agtcttttac agcccaagct
     7441 cagaagtgac atagcgtcac tattgtcata tgggtcacag gctgaccctg tgtgatgtgg
     7501 gaggggactg aacagggtgt gaatgccagg tgggggtcac cggggccttc gtgggagctg
     7561 gcagccacaa gaagcagctg cctggagaga gtggctggtc tctgagaaag gtcttgaggc
     7621 gataccatcc ccttggtaat tgtagagaat caggagaagt tacgttatct tcctgggcct
     7681 cattttatta tctgacaaat gaaggattgg tagaatgggt tccttcccat tctgagattc
     7741 tgattctgat tgcagcaaga atggacctac agtctacaga agagccacat caggacttgc
     7801 tgtgcatttc aactgagagg aaagctaatc gggataatta ttcccaaact gagctggact
     7861 aaatcactag ttaattctac aatattcagg gcttcattcc gcatgtttgg gaattgacga
     7921 cagttaacac tgaattactt tattttgaat tggacaaaat atgttgatag ctttaggttc
     7981 atttcaactt gattccaggc ttagtcttaa atctgtagtt aagttagagc atctgagtct
     8041 ttgggaagtt aagcttcaaa cacaagtaga ccagcagggt gtggcatggg cagggtggct
     8101 gggaagctat cctgctcctg gcacacttcc ctgccttctg ctgtgctccc agaggagcag
     8161 cctcacctct gagggtaagg ccaaaaatca gcctacactg agttacttgt gggtcaattg
     8221 ttcatataac ttggctcttc tgaactgtca tccatagtat caccgatgat caatctcagc
     8281 attggaagaa aaagaaacca cagagtccag cccctctgtc acaacttcag agtaaaggag
     8341 ttgctcatgc ctgccagcca gcctgtccca ccacagcggg ctccccagct tagggaggtc
     8401 tgtgagccat gtgctaaatt tctctttaga gctaaatcca ttgagtgcta tgattccagt
     8461 gctgtgcaga aagcagatgt ttcacctatc acttcagaaa agttcaccag acttcatttt
     8521 gacagctacc ttagatcatc aaggagaaaa aggaaaaaat gtaaaaacca gtggggctag
     8581 aaggtgagtt acagctcaga ttggggggcc tctggatagc aaagcaatga ctgagagcgt
     8641 cagccctacc caccccagaa gtgagctctg ggccgtgatg ccaggttgtc tgaaatcaca
     8701 gtatcttatt ttttctcttc tcttccctgc tctgcttctc atttcctaac atttacctgt
     8761 tccttacagt ccttcctttt aaataatatt tttaaaatac tgctttcctg aaaaccatgc
     8821 tgcacttcca catgaagttc tctctgacgt tgagcttgat ggaaaaagga tgcagaagtc
     8881 aggaatagca ggtgctatac ttattttggc cagcagagga tgcacttgcc ctaaacagtc
     8941 agagtgactc ttccctagga ccctgtttat caaattcttc atgtttaaaa gccagggtct
     9001 ggccccccac ttaacgagat tgtctcaagt ggcatttcat ctgtaactca attttacaga
     9061 aatctagaaa atacaggctc cccccaccct ctccagcccc aaaacacaaa gcgtagtaag
     9121 gctttcattg caagcatcag tctccatcta attccacttc cacatataaa gattttaaaa
     9181 tttcaattaa aaaagaatct atggttgatc ctttggttgg acaacagtaa gtaaatgttg
     9241 attttaaact agtgagtcaa ctactctggc aatgttggtg tatctttgga aagaagggaa
     9301 gtgaatttat tatgcaccat atgtccatgt gtggcacagc acttagctaa acagtatttt
     9361 ggagaaaata tttggtcttc aacacacacc tatataaaaa agattagttc tatttcttca
     9421 atatgaccta aacttcatct caagtccagt tggtaagaga actatgaacc tgtttttcct
     9481 ggtctgttgt acaacggttc ccttgtggac taaacggtaa tgtgttctgc atgaatccaa
     9541 gcacaactga cattcactgt tttaaaaaac aaaaaagaga aacatttact acctttttca
     9601 gatctcattt ctgatggacc tgaaaatatt gtaagtgggt attttcttca aatactcaga
     9661 gactgaattc taacatgtaa agaaaatgct gactcacaca tgactcagtt tgaaggttac
     9721 agggaagact aaacatttat ttagaagtta cagtgtttgt ttttacagag atatgttgcc
     9781 acggattatt gattctggaa ggttttttaa tgctcatagt gctgtacaca tcttggtctg
     9841 ttctcatggg catcttagga aaatgtttcc cgtagcaccg gatccagcca tctgtgtggg
     9901 tgtctccagg ctgtgatacc gtgccccttg ctgttccctc atgctcgtga tgaaccatgt
     9961 gcctgggatc cttcctcttg ccagcttatc tgtgggcctc ctgcagcctc gcctgccact
    10021 gcagaggccc caggttagga gtctgtcccg cgccagcctg tgcggtgctt tcctgggtga
    10081 ctcctgttag gtggcagctt ctttattttc cctctccaac ctcaacgggt aggactcctt
    10141 atgtctctcc agaattataa aacatataca tttatttaca agtgcataaa tatataaata
    10201 tggctataat agaaaaataa ataccaattt tctctgtc
//
