LOCUS       NM_004365               2406 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens centrin 3 (CETN3), transcript variant 2, mRNA.
ACCESSION   NM_004365
VERSION     NM_004365.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   O'Neill,A.C., Uzbas,F., Antognolli,G., Merino,F., Draganova,K.,
            Jack,A., Zhang,S., Pedini,G., Schessner,J.P., Cramer,K.,
            Schepers,A., Metzger,F., Esgleas,M., Smialowski,P., Guerrini,R.,
            Falk,S., Feederle,R., Freytag,S., Wang,Z., Bahlo,M., Jungmann,R.,
            Bagni,C., Borner,G.H.H., Robertson,S.P., Hauck,S.M. and Gotz,M.
  TITLE     Spatial centrosome proteome of human neural cells uncovers
            disease-relevant heterogeneity
  JOURNAL   Science 376 (6599), eabf9088 (2022)
   PUBMED   35709258
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2406)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2406)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2406)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2406)
  AUTHORS   Laoukili,J., Perret,E., Middendorp,S., Houcine,O., Guennou,C.,
            Marano,F., Bornens,M. and Tournier,F.
  TITLE     Differential expression and cellular distribution of centrin
            isoforms during human ciliated cell differentiation in vitro
  JOURNAL   J Cell Sci 113 (Pt 8), 1355-1364 (2000)
   PUBMED   10725219
REFERENCE   7  (bases 1 to 2406)
  AUTHORS   LeDizet,M., Beck,J.C. and Finkbeiner,W.E.
  TITLE     Differential regulation of centrin genes during ciliogenesis in
            human tracheal epithelial cells
  JOURNAL   Am J Physiol 275 (6), L1145-L1156 (1998)
   PUBMED   9843852
REFERENCE   8  (bases 1 to 2406)
  AUTHORS   Middendorp,S., Paoletti,A., Schiebel,E. and Bornens,M.
  TITLE     Identification of a new mammalian centrin gene, more closely
            related to Saccharomyces cerevisiae CDC31 gene
  JOURNAL   Proc Natl Acad Sci U S A 94 (17), 9141-9146 (1997)
   PUBMED   9256449
REFERENCE   9  (bases 1 to 2406)
  AUTHORS   Paoletti,A., Moudjou,M., Paintrand,M., Salisbury,J.L. and
            Bornens,M.
  TITLE     Most of centrin in animal cells is not centrosome-associated and
            centrosomal centrin is confined to the distal lumen of centrioles
  JOURNAL   J Cell Sci 109 (Pt 13), 3089-3102 (1996)
   PUBMED   9004043
REFERENCE   10 (bases 1 to 2406)
  AUTHORS   Wiech,H., Geier,B.M., Paschke,T., Spang,A., Grein,K.,
            Steinkotter,J., Melkonian,M. and Schiebel,E.
  TITLE     Characterization of green alga, yeast, and human centrins. Specific
            subdomain features determine functional diversity
  JOURNAL   J Biol Chem 271 (37), 22453-22461 (1996)
   PUBMED   8798410
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB998764.1, BC005383.1,
            BQ000279.1 and AC093510.2.
            
            On Feb 27, 2020 this sequence version replaced NM_004365.3.
            
            Summary: The protein encoded by this gene contains four EF-hand
            calcium binding domains, and is a member of the centrin protein
            family. Centrins are evolutionarily conserved proteins similar to
            the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the
            centrosome of interphase and mitotic cells, where it plays a
            fundamental role in centrosome duplication and separation. Multiple
            forms of the proteins similar to the yeast centrin have been
            identified in human and other mammalian cells, some of which have
            been shown to be associated with centrosome fractions. This protein
            appears to be one of the most abundant centrins associated with
            centrosome, which suggests a similar function to its yeast
            counterpart. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            3' coding region, compared to variant 1. The resulting isoform (2)
            lacks an internal segment, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005383.1, BI439055.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMN03267752
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000283122.8/ ENSP00000283122.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-617               CB998764.1         34-650
            618-942             BC005383.1         618-942
            943-1328            BQ000279.1         17-402              c
            1329-2406           AC093510.2         92840-93917
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.3"
     gene            1..2406
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="centrin 3"
                     /db_xref="GeneID:1070"
                     /db_xref="HGNC:HGNC:1868"
                     /db_xref="MIM:602907"
     exon            1..112
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..599
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     centrin, EF-hand protein, 3 (CDC31 homolog, yeast); CDC31
                     yeast homolog; EF-hand superfamily member"
                     /codon_start=1
                     /product="centrin-3 isoform 2"
                     /protein_id="NP_004356.2"
                     /db_xref="CCDS:CCDS4066.1"
                     /db_xref="GeneID:1070"
                     /db_xref="HGNC:HGNC:1868"
                     /db_xref="MIM:602907"
                     /translation="MSLALRSELVVDKTKRKKRRELSEEQKQEIKDAFELFDTDKDEA
                     IDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILERDPHEE
                     ILKAFKLFDDDDSGKISLRNLRRVARELGENMSDEELRAMIEEFDKDGDGEINQEEFI
                     AIMTGDI"
     exon            113..248
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            249..363
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            364..555
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /inference="alignment:Splign:2.1.0"
     exon            556..2406
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      819..824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="hexamer: TATAAA"
     polyA_site      839
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="major polyA site"
     regulatory      922..927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="hexamer: AATAAA"
     polyA_site      952
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="major polyA site"
     regulatory      1308..1313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="hexamer: AATACA"
     polyA_site      1328
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
     regulatory      2383..2388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
                     /note="hexamer: AATAAA"
     polyA_site      2406
                     /gene="CETN3"
                     /gene_synonym="CDC31; CEN3"
ORIGIN      
        1 gtcgttcgtg tctgttgaac ggctgtgggc gtcttgctgc cttgggtagg gggttaaaat
       61 cgttcttgag aggaacgtct ctgtgcgaag agataatgag tttagctctg agaagtgagc
      121 ttgtagtgga caaaacaaag aggaaaaaaa gaagagaact gtctgaggaa cagaaacaag
      181 aaattaaaga tgcttttgaa ctatttgata cagacaaaga tgaagcaata gattatcatg
      241 aattaaaggt ggcaatgaga gccttggggt ttgatgtaaa aaaagctgat gtactgaaga
      301 ttcttaaaga ttatgacaga gaagccacag ggaaaatcac ctttgaagat tttaatgaag
      361 ttgtgacaga ctggatattg gaaagagatc cccatgaaga aatactcaag gcatttaaac
      421 tatttgatga tgatgattca ggtaaaataa gcttgaggaa tttgcgacgt gttgctagag
      481 aattgggtga aaacatgagt gatgaagaac ttcgagctat gatagaagaa tttgacaaag
      541 atggtgatgg agaaataaac caagaggagt tcattgctat tatgactggt gacatttaaa
      601 gaattacaag gataaacact aagaatgttg cagttaccat cttatattct atttttgtgc
      661 ctggagccat gtgaaaaaaa ccaacttagt tcttttatcc taaaggacca aaaataagca
      721 tcttatatat ctgtatttta ctactgttaa gtttctttgt atgaactgtg ttgttagtac
      781 tcagatagtt tagctttgta tttataatag agcttttata taaagtttta aaaattgaat
      841 gtgtgatatg tgttctttga aggtttttta atttaacatt tatagtcact ttttagtgca
      901 cacattttcc agactttcgt taataaaata tttattttat tagaatttta aagtttggta
      961 aatttgtttt tattcctagt atctgttaac cctcaagagt ggatttctta aatcttccct
     1021 ttataatttt actagtttct gaaacaaaaa ttgaaataca attagcaata gagtggcaca
     1081 tttttccatt acctaacatt tcatcaattt tgacattcta tattttaata caagctgact
     1141 caatataagc ctaattcctt ttttaagagt cctgtctttg agtttagaag gaataagtat
     1201 tgtgcagata gacccattta tccttgtgta ttatgtttct taacttggta tcattgtaag
     1261 tatgaaagtt aattttcttc tgaatatttc cttaaacaaa ttgaatgaat acaatttgaa
     1321 ttttctaact tcttgactga gagatttaaa ttttggaatt ttgatttgtt aaggtaatta
     1381 tcactcaatt acatgtcaca gtgatgaaaa tagaaaactg taagccacag aaataattta
     1441 agtctatgtt ttacctatat gaaggaacag tctgtgattc accaggaaat agtagagaca
     1501 cagtttccaa gtactggtgg gctcttctgc cttgtagaag tgtatgttag ggggaaacaa
     1561 gtggttatgg agatcgatca tagaaaaaaa tagtacctat atggatcctt cactcatatc
     1621 tagtactcgg tgttatgggc tctcccagga acggaattga gttgtctatt tcagcaatct
     1681 ttctttagaa ttcttgtttt aaatttttaa gtttaaagtg tagtatcatt tgaggcgctt
     1741 taaatgttgt gagcctttta agagtattac attaagggtt gtccactaga aatttaacac
     1801 aaaatcagta gggaatacta tagcagtagt gacatttcaa acagttatct aggaagcaag
     1861 tgtatattaa gtcattcttg tattgctacg aagaaatacc caagactgca tattttataa
     1921 agaaaagaag cttaattggc ttacggttct gcaggcttta caggaagcat ggtgcttctg
     1981 cttttgggga ggcctcagga agcttacagt catggtagaa ggcaaagggg gagcaggcac
     2041 atcacatggt gaaaaccaga gtgaacaagt gagagaagtg ccaagtactt tgaaaccacc
     2101 agatctcatg agaactcact attgtgagga catctaatca cctcccacca gacctcacct
     2161 ccagtactgg ggattacaat tcaacatgat atttgagtgg ggacagatat acaaactata
     2221 tcagcaggtt aactagagta aaacttaatt ttgaatacaa attcaatggt cacagtcatt
     2281 atattcaggg caacagacct tgaaattaaa aaatttttgt cgcacactga tgagttccct
     2341 ctgtcttgat catgaaatat attgatttcc aggaaagtga aaaataaaac agtaaaagta
     2401 aatcta
//
