LOCUS       NM_024922               3858 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens carboxylesterase 3 (CES3), transcript variant 1, mRNA.
ACCESSION   NM_024922
VERSION     NM_024922.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Zhao,B., Bie,J., Wang,J., Marqueen,S.A. and Ghosh,S.
  TITLE     Identification of a novel intracellular cholesteryl ester hydrolase
            (carboxylesterase 3) in human macrophages: compensatory increase in
            its expression after carboxylesterase 1 silencing
  JOURNAL   Am J Physiol Cell Physiol 303 (4), C427-C435 (2012)
   PUBMED   22700792
  REMARK    GeneRIF: This study provides the first evidence of functional
            compensation whereby increased expression of CES3 restores
            intracellular cholesteryl ester hydrolytic activity and free
            cholesterol efflux in CES1-deficient cells.
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Holmes,R.S., Wright,M.W., Laulederkind,S.J., Cox,L.A., Hosokawa,M.,
            Imai,T., Ishibashi,S., Lehner,R., Miyazaki,M., Perkins,E.J.,
            Potter,P.M., Redinbo,M.R., Robert,J., Satoh,T., Yamashita,T.,
            Yan,B., Yokoi,T., Zechner,R. and Maltais,L.J.
  TITLE     Recommended nomenclature for five mammalian carboxylesterase gene
            families: human, mouse, and rat genes and proteins
  JOURNAL   Mamm Genome 21 (9-10), 427-441 (2010)
   PUBMED   20931200
REFERENCE   3  (bases 1 to 3858)
  AUTHORS   Howard,T.D., Hsu,F.C., Grzywacz,J.G., Chen,H., Quandt,S.A.,
            Vallejos,Q.M., Whalley,L.E., Cui,W., Padilla,S. and Arcury,T.A.
  TITLE     Evaluation of candidate genes for cholinesterase activity in
            farmworkers exposed to organophosphorus pesticides: association of
            single nucleotide polymorphisms in BCHE
  JOURNAL   Environ Health Perspect 118 (10), 1395-1399 (2010)
   PUBMED   20529763
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   4  (bases 1 to 3858)
  AUTHORS   Holmes,R.S., Cox,L.A. and VandeBerg,J.L.
  TITLE     Mammalian carboxylesterase 3: comparative genomics and proteomics
  JOURNAL   Genetica 138 (7), 695-708 (2010)
   PUBMED   20422440
  REMARK    GeneRIF: Studies indicate that CES3 mRNA isoform expression in
            several tissues, particularly in colon, trachea and in brain.
REFERENCE   5  (bases 1 to 3858)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   6  (bases 1 to 3858)
  AUTHORS   Sanghani,S.P., Sanghani,P.C., Schiel,M.A. and Bosron,W.F.
  TITLE     Human carboxylesterases: an update on CES1, CES2 and CES3
  JOURNAL   Protein Pept Lett 16 (10), 1207-1214 (2009)
   PUBMED   19508181
  REMARK    GeneRIF: CES3 seems to have a lower catalytic efficiency than the
            other two CESs for selected substrates.
            Review article
REFERENCE   7  (bases 1 to 3858)
  AUTHORS   Fukami,T., Nakajima,M., Maruichi,T., Takahashi,S., Takamiya,M.,
            Aoki,Y., McLeod,H.L. and Yokoi,T.
  TITLE     Structure and characterization of human carboxylesterase 1A1, 1A2,
            and 1A3 genes
  JOURNAL   Pharmacogenet Genomics 18 (10), 911-920 (2008)
   PUBMED   18794728
  REMARK    GeneRIF: pharmacogenomic characterization of human carboxylesterase
            1A1, 1A2, and 1A3 genes
REFERENCE   8  (bases 1 to 3858)
  AUTHORS   Sanghani,S.P., Quinney,S.K., Fredenburg,T.B., Davis,W.I.,
            Murry,D.J. and Bosron,W.F.
  TITLE     Hydrolysis of irinotecan and its oxidative metabolites,
            7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]
            carbonyloxycamptothecin and
            7-ethyl-10-[4-(1-piperidino)-1-amino]-carbonyloxycamptothecin, by
            human carboxylesterases CES1A1, CES2, and a newly expressed
            carboxylesterase isoenzyme, CES3
  JOURNAL   Drug Metab Dispos 32 (5), 505-511 (2004)
   PUBMED   15100172
REFERENCE   9  (bases 1 to 3858)
  AUTHORS   Sanghani,S.P., Quinney,S.K., Fredenburg,T.B., Sun,Z., Davis,W.I.,
            Murry,D.J., Cummings,O.W., Seitz,D.E. and Bosron,W.F.
  TITLE     Carboxylesterases expressed in human colon tumor tissue and their
            role in CPT-11 hydrolysis
  JOURNAL   Clin Cancer Res 9 (13), 4983-4991 (2003)
   PUBMED   14581373
REFERENCE   10 (bases 1 to 3858)
  AUTHORS   Aida,K., Moore,R. and Negishi,M.
  TITLE     Cloning and nucleotide sequence of a novel, male-predominant
            carboxylesterase in mouse liver
  JOURNAL   Biochim Biophys Acta 1174 (1), 72-74 (1993)
   PUBMED   7916639
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA924627.1, AK000105.1 and
            AK025158.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024922.5.
            
            Summary: This gene encodes a member of the carboxylesterase large
            family. The family members are responsible for the hydrolysis or
            transesterification of various xenobiotics, such as cocaine and
            heroin, and endogenous substrates with ester, thioester, or amide
            bonds. They may participate in fatty acyl and cholesterol ester
            metabolism, and may play a role in the blood-brain barrier system.
            This gene is expressed in several tissues, particularly in colon,
            trachea and in brain, and the protein participates in colon and
            neural drug metabolism. Multiple alternatively spliced transcript
            variants encoding distinct isoforms have been reported, but the
            biological validity and/or full-length nature of some variants have
            not been determined.[provided by RefSeq, Jun 2010].
            
            Transcript Variant: This variant (1) is the longest transcript and
            encodes the longest isoform (1, also known as isoform a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358609.1, AK000105.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000303334.9/ ENSP00000304782.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                DA924627.1         38-61
            25-2117             AK000105.1         1-2093
            2118-3858           AK025158.1         737-2477
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..3858
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /note="carboxylesterase 3"
                     /db_xref="GeneID:23491"
                     /db_xref="HGNC:HGNC:1865"
                     /db_xref="MIM:605279"
     exon            1..124
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..1758
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /EC_number="3.1.1.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; carboxylesterase 3 (brain); esterase 31; liver
                     carboxylesterase 31 homolog"
                     /codon_start=1
                     /product="carboxylesterase 3 isoform 1 precursor"
                     /protein_id="NP_079198.2"
                     /db_xref="CCDS:CCDS10826.1"
                     /db_xref="GeneID:23491"
                     /db_xref="HGNC:HGNC:1865"
                     /db_xref="MIM:605279"
                     /translation="MERAVRVESGVLVGVVCLLLACPATATGPEVAQPEVDTTLGRVR
                     GRQVGVKGTDRLVNVFLGIPFAQPPLGPDRFSAPHPAQPWEGVRDASTAPPMCLQDVE
                     SMNSSRFVLNGKQQIFSVSEDCLVLNVYSPAEVPAGSGRPVMVWVHGGALITGAATSY
                     DGSALAAYGDVVVVTVQYRLGVLGFFSTGDEHAPGNQGFLDVVAALRWVQENIAPFGG
                     DLNCVTVFGGSAGGSIISGLVLSPVAAGLFHRAITQSGVITTPGIIDSHPWPLAQKIA
                     NTLACSSSSPAEMVQCLQQKEGEELVLSKKLKNTIYPLTVDGTVFPKSPKELLKEKPF
                     HSVPFLMGVNNHEFSWLIPRGWGLLDTMEQMSREDMLAISTPVLTSLDVPPEMMPTVI
                     DEYLGSNSDAQAKCQAFQEFMGDVFINVPTVSFSRYLRDSGSPVFFYEFQHRPSSFAK
                     IKPAWVKADHGAEGAFVFGGPFLMDESSRLAFPEATEEEKQLSLTMMAQWTHFARTGD
                     PNSKALPPWPQFNQAEQYLEINPVPRAGQKFREAWMQFWSETLPSKIQQWHQKQKNRK
                     AQEDL"
     exon            125..329
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            330..468
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            469..602
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            603..756
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            757..861
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            862..963
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1104
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1185
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1333
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1483
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1562
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..3858
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3838..3843
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /note="hexamer: AATAAA"
     polyA_site      3858
                     /gene="CES3"
                     /gene_synonym="ES31"
                     /note="major polyA site"
ORIGIN      
        1 attccacctt ctgaagcttc tgtcgaacca gttgtaagga gaatggagag agcagtgaga
       61 gtggagtccg gggtcctggt cggggtggtc tgtctgctcc tggcatgccc tgccacagcc
      121 actgggcccg aagttgctca gcctgaagta gacaccaccc tgggtcgtgt gcgaggccgg
      181 caggtgggcg tgaagggcac agaccgcctt gtgaatgtct ttctgggcat tccatttgcc
      241 cagccgccac tgggccctga ccggttctca gccccacacc cagcacagcc ctgggagggt
      301 gtgcgggatg ccagcactgc gcccccaatg tgcctacaag acgtggagag catgaacagc
      361 agcagatttg tcctcaacgg aaaacagcag atcttctccg tttcagagga ctgcctggtc
      421 ctcaacgtct atagcccagc tgaggtcccc gcagggtccg gtaggccggt catggtatgg
      481 gtccatggag gcgctctgat aactggcgct gccacctcct acgatggatc agctctggct
      541 gcctatgggg atgtggtcgt ggttacagtc cagtaccgcc ttggggtcct tggcttcttc
      601 agcactggag atgagcatgc acctggcaac cagggcttcc tagatgtggt agctgctttg
      661 cgctgggtgc aagaaaacat cgcccccttc gggggtgacc tcaactgtgt cactgtcttt
      721 ggtggatctg ccggtgggag catcatctct ggcctggtcc tgtccccagt ggctgcaggg
      781 ctgttccaca gagccatcac acagagtggg gtcatcacca ccccagggat catcgactct
      841 cacccttggc ccctagctca gaaaatcgca aacaccttgg cctgcagctc cagctccccg
      901 gctgagatgg tgcagtgcct tcagcagaaa gaaggagaag agctggtcct tagcaagaag
      961 ctgaaaaata ctatctatcc tctcaccgtt gatggcactg tcttccccaa aagccccaag
     1021 gaactcctga aggagaagcc cttccactct gtgcccttcc tcatgggtgt caacaaccat
     1081 gagttcagct ggctcatccc caggggctgg ggtctcctgg atacaatgga gcagatgagc
     1141 cgggaggaca tgctggccat ctcaacaccc gtcttgacca gtctggatgt gccccctgag
     1201 atgatgccca ccgtcataga tgaataccta ggaagcaact cggacgcaca agccaaatgc
     1261 caggcgttcc aggaattcat gggtgacgta ttcatcaatg ttcccaccgt cagtttttca
     1321 agataccttc gagattctgg aagccctgtc tttttctatg agttccagca tcgacccagt
     1381 tcttttgcga agatcaaacc tgcctgggtg aaggctgatc atggggccga gggtgctttt
     1441 gtgttcggag gtcccttcct catggacgag agctcccgcc tggcctttcc agaggccaca
     1501 gaggaggaga agcagctaag cctcaccatg atggcccagt ggacccactt tgcccggaca
     1561 ggggacccca atagcaaggc tctgcctcct tggccccaat tcaaccaggc ggaacaatat
     1621 ctggagatca acccagtgcc acgggccgga cagaagttca gggaggcctg gatgcagttc
     1681 tggtcagaga cgctccccag caagatacaa cagtggcacc agaagcagaa gaacaggaag
     1741 gcccaggagg acctctgagg ccaggcctga accttcttgg ctggggcaaa ccactcttca
     1801 agtggtggca gagtcccagc acggcagccc gcctctcccc ctgctgagac tttaatctcc
     1861 accagccctt aaagtgtcgg ccgctctgtg actggagtta tgctcttttg aaatgtcaca
     1921 aggccgcctc ccacctctgg ggcattgtac aagttcttcc ctctccctga agtgcctttc
     1981 ctgctttctt cgtggtaggt tctagcacat tcctctagct tcctggagga ctcactcccc
     2041 caggaagcct tccctgcctt ctctgggctg tgcggccccg agtctgcgtc cattagagca
     2101 cagtccaccc gaggctagca ccgtgtctgt gtctgtctcc ccctcagagg agctctctca
     2161 aaatggggat tagcctaacc ccactctgtc acccacacca ggatcgggtg ggacctggag
     2221 ctagggggtg tttgctgagt gagtgagtga aacacagaat atgggaatgg cagctgctga
     2281 acttgaaccc agagccttca ggtgccaaag ccatactcag gcccccaccg acattgtcca
     2341 ccctggccag aagggtgcat gccaatggca gagacctggg atgggagaag tcctggggcg
     2401 ccaggggatc cagcctagag cagaccttag cccctgacta aggcctcaga ctagggcggg
     2461 aggggtctcc tcctctctgc tgcccagtcc tggcccctgc acaagacaac agaatccatc
     2521 agggccatga gtgtcaccca gacctgaccc tcaccaattc cagccctgac cctcaggacg
     2581 ctggatgcca gctcccagcc ccagtgccgg gtcctccctc ccttcctggc ttggggagac
     2641 cagtttctgg ggagcttcca agagcaccca ccaagacaca gcaggacagg ccaggggagg
     2701 gcatctggac cagggcatcc gtcgggctat tgtcacagag aaaagaagag acccacccac
     2761 tcgggctgca aaaggtgaaa agcaccaaga ggttttcaga tggaagtgag aggtgacagt
     2821 gtgctggcag ccctcacagc cctcgcttgc tctccctgcc gcctctgcct gggctcccac
     2881 tttggcagca cttgaggagc ccttcaaccc gccgctgcac tgtaggagcc cctttctggg
     2941 ctggccaagg ccggagccag ctccctcagc ttgcggggag gtgcggaggg agaggggcgg
     3001 gcaggaaccg gggctgcgcg cagcgcttgc gggccagagt gagttccggg tgggcgtggg
     3061 ctcggcgggg ccccactcag agcagctggc cggccccagg cagtgagggc cttagcacct
     3121 gggccagcag ctgctgtgct cgatttctcg ctgggcctta gctgcctccc cgcggggcag
     3181 ggctcgggac ctgcagccct ccatgcctga ccctcccccc accccccgtg ggctcctgtg
     3241 cggccggagc ctccccaagg agcgccgccc cctgctccac agcgcccagt cccatcgacc
     3301 acccaagggc tgaggagtgc gggtgcacag cgcgggactg gcaggcagct ccacctgctg
     3361 ccccagtgct ggatccactg ggtgaagcca gctgggctcc tgagtctggt ggggacttgg
     3421 agaaccttta tgtctagcta agggattgta aatacaccga tgggcactct gtatctagct
     3481 caaggtttgt aaacacacca atcagcaccc tgtgtctagc tcagtgtttg tgaatgcacc
     3541 aatccacact ctgtatctgg ctactctggt ggggacttgg agaacctttg tgtccacact
     3601 ctgtatctag ctaatctagt ggggatgtgg agaacctttg tgtctagctc agggatcgta
     3661 aacgcaccaa tcagcaccct gtcaaaacag accacttgac tctctgtaaa atggaccaat
     3721 cagcaggatg tgggtggggc gagacaagag aataaaagca ggctgcctga gccagcagtg
     3781 acaacccccc tcgggtcccc tcccacgccg tggaagcttt gttctttcgc tctttgcaat
     3841 aaatcttgct actgccca
//
