LOCUS       NM_022075               2323 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens ceramide synthase 2 (CERS2), transcript variant 2,
            mRNA.
ACCESSION   NM_022075
VERSION     NM_022075.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Heinitz,S., Traurig,M., Krakoff,J., Rabe,P., Staubert,C., Kobes,S.,
            Hanson,R.L., Stumvoll,M., Bluher,M., Bogardus,C., Baier,L. and
            Piaggi,P.
  TITLE     An E115A Missense Variant in CERS2 Is Associated With Increased
            Sleeping Energy Expenditure and Hepatic Insulin Resistance in
            American Indians
  JOURNAL   Diabetes 73 (8), 1361-1371 (2024)
   PUBMED   38776413
  REMARK    GeneRIF: An E115A Missense Variant in CERS2 Is Associated With
            Increased Sleeping Energy Expenditure and Hepatic Insulin
            Resistance in American Indians.
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Shi,H., Tan,Z., Duan,B., Guo,C., Li,C., Luan,T., Li,N., Huang,Y.,
            Chen,S., Gao,J., Feng,W., Xu,H., Wang,J., Fu,S. and Wang,H.
  TITLE     LASS2 enhances chemosensitivity to cisplatin by inhibiting
            PP2A-mediated beta-catenin dephosphorylation in a subset of
            stem-like bladder cancer cells
  JOURNAL   BMC Med 22 (1), 19 (2024)
   PUBMED   38191448
  REMARK    GeneRIF: LASS2 enhances chemosensitivity to cisplatin by inhibiting
            PP2A-mediated beta-catenin dephosphorylation in a subset of
            stem-like bladder cancer cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2323)
  AUTHORS   Liu,Z., Xuan,C., Han,X., Zheng,Z., Xiao,R., Bao,L. and Xu,X.
  TITLE     [LASS2/TMSG1 overexpression inhibits proliferation and promotes
            apoptosis of human lung cancer A549 cells possibly by upregulating
            ceramide and p38 MAPK to activate a signaling cascade]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (2), 166-174 (2023)
   PUBMED   36946034
  REMARK    GeneRIF: [LASS2/TMSG1 overexpression inhibits proliferation and
            promotes apoptosis of human lung cancer A549 cells possibly by
            upregulating ceramide and p38 MAPK to activate a signaling
            cascade].
REFERENCE   4  (bases 1 to 2323)
  AUTHORS   Wang,Y., Li,S., Weng,L., Du,H., Wang,J. and Xu,X.
  TITLE     LASS2 overexpression enhances early apoptosis of lung cancer cells
            through the caspase-dependent pathway
  JOURNAL   Oncol Rep 48 (6) (2022)
   PUBMED   36300249
  REMARK    GeneRIF: LASS2 overexpression enhances early apoptosis of lung
            cancer cells through the caspasedependent pathway.
REFERENCE   5  (bases 1 to 2323)
  AUTHORS   Shao,X., Zheng,Y., Huang,Y., Li,G., Zou,W. and Shi,L.
  TITLE     Hsa-miR-221-3p promotes proliferation and migration in
            HER2-positive breast cancer cells by targeting LASS2 and MBD2
  JOURNAL   Histol Histopathol 37 (11), 1099-1112 (2022)
   PUBMED   35734966
  REMARK    GeneRIF: Hsa-miR-221-3p promotes proliferation and migration in
            HER2-positive breast cancer cells by targeting LASS2 and MBD2.
REFERENCE   6  (bases 1 to 2323)
  AUTHORS   Laviad,E.L., Albee,L., Pankova-Kholmyansky,I., Epstein,S., Park,H.,
            Merrill,A.H. Jr. and Futerman,A.H.
  TITLE     Characterization of ceramide synthase 2: tissue distribution,
            substrate specificity, and inhibition by sphingosine 1-phosphate
  JOURNAL   J Biol Chem 283 (9), 5677-5684 (2008)
   PUBMED   18165233
  REMARK    GeneRIF: activity of CerS2 can be regulated by another bioactive
            sphingolipid, sphingosine 1-phosphate
REFERENCE   7  (bases 1 to 2323)
  AUTHORS   Lahiri,S., Lee,H., Mesicek,J., Fuks,Z., Haimovitz-Friedman,A.,
            Kolesnick,R.N. and Futerman,A.H.
  TITLE     Kinetic characterization of mammalian ceramide synthases:
            determination of K(m) values towards sphinganine
  JOURNAL   FEBS Lett 581 (27), 5289-5294 (2007)
   PUBMED   17977534
REFERENCE   8  (bases 1 to 2323)
  AUTHORS   Lewandrowski,U., Moebius,J., Walter,U. and Sickmann,A.
  TITLE     Elucidation of N-glycosylation sites on human platelet proteins: a
            glycoproteomic approach
  JOURNAL   Mol Cell Proteomics 5 (2), 226-233 (2006)
   PUBMED   16263699
REFERENCE   9  (bases 1 to 2323)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   10 (bases 1 to 2323)
  AUTHORS   Pan,H., Qin,W.X., Huo,K.K., Wan,D.F., Yu,Y., Xu,Z.G., Hu,Q.D.,
            Gu,K.T., Zhou,X.M., Jiang,H.Q., Zhang,P.P., Huang,Y., Li,Y.Y. and
            Gu,J.R.
  TITLE     Cloning, mapping, and characterization of a human homologue of the
            yeast longevity assurance gene LAG1
  JOURNAL   Genomics 77 (1-2), 58-64 (2001)
   PUBMED   11543633
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL590133.32.
            
            On Jun 3, 2019 this sequence version replaced NM_022075.4.
            
            Summary: This gene encodes a protein that has sequence similarity
            to yeast longevity assurance gene 1. Mutation or overexpression of
            the related gene in yeast has been shown to alter yeast lifespan.
            The human protein may play a role in the regulation of cell growth.
            Alternatively spliced transcript variants encoding the same protein
            have been described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' UTR compared to variant 1. Variants 1 and 2 encode the
            same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC001357.2, AY091458.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368954.10/ ENSP00000357950.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-217               AL590133.32        60812-61028         c
            218-391             AL590133.32        55111-55284         c
            392-509             AL590133.32        54588-54705         c
            510-628             AL590133.32        54276-54394         c
            629-686             AL590133.32        54013-54070         c
            687-737             AL590133.32        53857-53907         c
            738-830             AL590133.32        53585-53677         c
            831-959             AL590133.32        53267-53395         c
            960-1066            AL590133.32        52949-53055         c
            1067-1220           AL590133.32        52669-52822         c
            1221-2323           AL590133.32        51379-52481         c
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.3"
     gene            1..2323
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /note="ceramide synthase 2"
                     /db_xref="GeneID:29956"
                     /db_xref="HGNC:HGNC:14076"
                     /db_xref="MIM:606920"
     exon            1..217
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            218..391
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..1361
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /EC_number="2.3.1.24"
                     /EC_number="2.3.1.297"
                     /note="longevity assurance (LAG1, S. cerevisiae) homolog
                     2; LAG1 homolog, ceramide synthase 2; LAG1 longevity
                     assurance 2; tumor metastasis-suppressor gene 1 protein;
                     sphingosine N-acyltransferase CERS2; very-long-chain
                     ceramide synthase CERS2"
                     /codon_start=1
                     /product="ceramide synthase 2"
                     /protein_id="NP_071358.1"
                     /db_xref="CCDS:CCDS973.1"
                     /db_xref="GeneID:29956"
                     /db_xref="HGNC:HGNC:14076"
                     /db_xref="MIM:606920"
                     /translation="MLQTLYDYFWWERLWLPVNLTWADLEDRDGRVYAKASDLYITLP
                     LALLFLIVRYFFELYVATPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVEL
                     LSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTFYLIAFIAGMAVIVDKPWF
                     YDMKKVWEGYPIQSTIPSQYWYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATII
                     LISFSWFANYIRAGTLIMALHDSSDYLLESAKMFNYAGWKNTCNNIFIVFAIVFIITR
                     LVILPFWILHCTLVYPLELYPAFFGYYFFNSMMGVLQLLHIFWAYLILRMAHKFITGK
                     LVEDERSDREETESSEGEEAAAGGGAKSRPLANGHPILNNNHRKND"
     exon            392..509
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            510..628
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            629..686
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            687..737
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            738..830
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..959
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1066
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1220
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..2323
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2230..2235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /note="hexamer: AATAAA"
     polyA_site      2250
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /note="major polyA site"
     regulatory      2300..2305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /note="hexamer: AATAAA"
     polyA_site      2323
                     /gene="CERS2"
                     /gene_synonym="L3; LASS2; SP260; TMSG1"
                     /note="major polyA site"
ORIGIN      
        1 ccggctgggc tcgggctcag ctcgactggg ctcggcgggc ggcggcggcg gcgccggcgg
       61 ctggcggagg agggagggcg agggcgggcg cgggccggcg ggcgggcgga agagggagga
      121 gaggcgcggg gagccaggcc tcggggcctc ggagcaacca cccgagcaga cggagtacac
      181 ggagcagcgg ccccggcccc gccaacgctg ccgccgggat gctccagacc ttgtatgatt
      241 acttctggtg ggaacgtctg tggctgcctg tgaacttgac ctgggccgat ctagaagacc
      301 gagatggacg tgtctacgcc aaagcctcag atctctatat cacgctgccc ctggccttgc
      361 tcttcctcat cgttcgatac ttctttgagc tgtacgtggc tacaccactg gctgccctct
      421 tgaacataaa ggagaaaact cggctgcggg cacctcccaa cgccaccttg gaacatttct
      481 acctgaccag tggcaagcag cccaagcagg tggaagtaga gcttttgtcc cggcagagcg
      541 ggctctctgg ccgccaggta gagcgttggt tccgtcgccg ccgcaaccag gaccggccca
      601 gtctcctcaa gaagttccga gaagccagct ggagattcac attttacctg attgccttca
      661 ttgccggcat ggccgtcatt gtggataaac cctggttcta tgacatgaag aaagtttggg
      721 agggatatcc catacagagc actatccctt cccagtattg gtactacatg attgaacttt
      781 ccttctactg gtccctgctc ttcagcattg cctctgatgt caagcgaaag gatttcaagg
      841 aacagatcat ccaccatgtg gccaccatca ttctcatcag cttttcctgg tttgccaatt
      901 acatccgagc tgggactcta atcatggctc tgcatgactc ttccgattac ctgctggagt
      961 cagccaagat gtttaactac gcgggatgga agaacacctg caacaacatc ttcatcgtct
     1021 tcgccattgt ttttatcatc acccgactgg tcatcctgcc cttctggatc ctgcattgca
     1081 ccctggtgta cccactggag ctctatcctg ccttctttgg ctattacttc ttcaattcca
     1141 tgatgggagt tctacagctg ctgcatatct tctgggccta cctcattttg cgcatggccc
     1201 acaagttcat aactggaaag ctggtagaag atgaacgcag tgaccgggaa gaaacagaga
     1261 gctcagaggg ggaggaggct gcagctgggg gaggagcaaa gagccggccc ctagccaatg
     1321 gccaccccat cctcaataac aaccatcgta agaatgactg aaccattact ccagctgcct
     1381 cccagattaa tgcataaagc caaggaacta ccccgctccc tgcgctatag ggtcacttta
     1441 agctctgggg aaaaaggaga aagtgagagg agagttctct gcatcctccc tccttgcttg
     1501 tcacccagtt gcctttaaac caaattctaa ccagcctatc cccaggtagg gggacgttgg
     1561 ttatattctg ttagaggggg acggtcgtat tttcctccct acccgccaag tcatcctttc
     1621 tactgctttt gaggccctcc ctcagctctc tgtgggtagg ggttacaatt cacattcctt
     1681 attctgagaa tttggcccca gctgtttgcc tttgactccc tgacctccag agccagggtt
     1741 gtgccttatt gtcccatctg tgggcctcat tctgccaaag ctggaccaag gctaaccttt
     1801 ctaagctccc taacttgggc cagaaaccaa agctgagctt ttaactttct ccctctatga
     1861 cacaaatgaa ttgagggtag gaggagggtg cacataaccc ttaccctacc tctgccaaaa
     1921 agtgggggct gtactgggga ctgctcggat gatctttctt agtgctactt ctttcagctg
     1981 tccctgtagc gacaggtcta agatctgact gcctcctcct ttctctggcc tcttccccct
     2041 tccctcttct cttcagctag gctagctggt ttggagtaga atggcaacta attctaattt
     2101 ttatttatta aatatttggg gttttggttt taaagccaga attacggcta gcacctagca
     2161 tttcagcaga gggaccattt tagaccaaaa tgtactgtta atgggttttt tttaaaaatt
     2221 aaaagattaa ataaaaaata ttaaataaaa catggcaata agtgtcagac tattaggaat
     2281 tgagaagggg gatcaactaa ataaacgaag agagtctttc tta
//
