LOCUS       NM_001319944            3934 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens centrosomal protein 85 (CEP85), transcript variant 4,
            mRNA.
ACCESSION   NM_001319944 XM_005245969
VERSION     NM_001319944.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Liu,Y., Kim,J., Philip,R., Sridhar,V., Chandrashekhar,M.,
            Moffat,J., van Breugel,M. and Pelletier,L.
  TITLE     Direct interaction between CEP85 and STIL mediates PLK4-driven
            directed cell migration
  JOURNAL   J Cell Sci 133 (8) (2020)
   PUBMED   32107292
  REMARK    GeneRIF: Direct interaction between CEP85 and STIL mediates
            PLK4-driven directed cell migration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3934)
  AUTHORS   Liu,Y., Gupta,G.D., Barnabas,D.D., Agircan,F.G., Mehmood,S., Wu,D.,
            Coyaud,E., Johnson,C.M., McLaughlin,S.H., Andreeva,A.,
            Freund,S.M.V., Robinson,C.V., Cheung,S.W.T., Raught,B.,
            Pelletier,L. and van Breugel,M.
  TITLE     Direct binding of CEP85 to STIL ensures robust PLK4 activation and
            efficient centriole assembly
  JOURNAL   Nat Commun 9 (1), 1731 (2018)
   PUBMED   29712910
  REMARK    GeneRIF: Direct binding of CEP85 to STIL ensures robust PLK4
            activation and efficient centriole assembly.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3934)
  AUTHORS   Chen,C., Tian,F., Lu,L., Wang,Y., Xiao,Z., Yu,C. and Yu,X.
  TITLE     Characterization of Cep85 - a new antagonist of Nek2A that is
            involved in the regulation of centrosome disjunction
  JOURNAL   J Cell Sci 128 (17), 3290-3303 (2015)
   PUBMED   26220856
  REMARK    GeneRIF: Cep85 is a bona fide Nek2A-binding partner that surrounds
            the proximal ends of centrioles where it cooperates with PP1gamma
            (also known as PPP1CC) to antagonize Nek2A
            Erratum:[J Cell Sci. 2015 Oct 15;128(20):3837. doi:
            10.1242/jcs.180463.. PMID: 26471995]
REFERENCE   5  (bases 1 to 3934)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   6  (bases 1 to 3934)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   7  (bases 1 to 3934)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   8  (bases 1 to 3934)
  AUTHORS   de Chassey,B., Navratil,V., Tafforeau,L., Hiet,M.S., Aublin-Gex,A.,
            Agaugue,S., Meiffren,G., Pradezynski,F., Faria,B.F., Chantier,T.,
            Le Breton,M., Pellet,J., Davoust,N., Mangeot,P.E., Chaboud,A.,
            Penin,F., Jacob,Y., Vidalain,P.O., Vidal,M., Andre,P.,
            Rabourdin-Combe,C. and Lotteau,V.
  TITLE     Hepatitis C virus infection protein network
  JOURNAL   Mol Syst Biol 4, 230 (2008)
   PUBMED   18985028
REFERENCE   9  (bases 1 to 3934)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB079544.1, AK024038.1,
            AK299312.1, AK025653.1 and BC064528.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001319944.1.
            
            Summary: This gene encodes a protein that belongs to the
            centrosome-associated family of proteins. The centrosome is a
            subcellular organelle in the animal cell that functions as a
            microtubule organizing center and is involved in cell-cycle
            progression. Alternate splicing results in multiple transcript
            variants. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (4) uses an alternate in-frame
            splice junction in the 3' end compared to variant 1. The resulting
            isoform (4) has the same N- and C-termini but is 1 aa shorter
            compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.79741.1,
                                           SRR1803617.148665.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000451429.8/ ENSP00000417002.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                DB079544.1         49-103
            56-905              AK024038.1         56-905
            906-1682            AK299312.1         753-1529
            1683-2627           AK024038.1         1686-2630
            2628-3156           AK025653.1         1277-1805
            3157-3934           BC064528.1         3158-3935
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..3934
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /note="centrosomal protein 85"
                     /db_xref="GeneID:64793"
                     /db_xref="HGNC:HGNC:25309"
                     /db_xref="MIM:618898"
     exon            1..111
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            112..188
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..2419
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /note="isoform 4 is encoded by transcript variant 4;
                     centrosomal protein of 85 kDa; coiled-coil
                     domain-containing protein 21; centrosomal protein 85kDa"
                     /codon_start=1
                     /product="centrosomal protein of 85 kDa isoform 4"
                     /protein_id="NP_001306873.1"
                     /db_xref="CCDS:CCDS81285.1"
                     /db_xref="GeneID:64793"
                     /db_xref="HGNC:HGNC:25309"
                     /db_xref="MIM:618898"
                     /translation="MAMQEKYPTEGISHVTSPSSDVIQKGSSLGTEWQTPVISEPFRS
                     RFSRCSSVADSGDTAIGTSCSDIAEDFCSSSGSPPFQPIKSHVTIPTAHVMPSTLGTS
                     PAKPNSTPVGPSSSKLPLSGLAESVGMTRNGDLGAMKHSPGLSRDLMYFSGATGENGI
                     EQSWFPAVGHERQEEARKFDIPSMESTLNQSAMMETLYSDPHHRVRFHNPRTSTSKEL
                     YRVLPEAKKAPGSGAVFERNGPHSNSSGVLPLGLQPAPGLSKPLPSQVWQPSPDTWHP
                     REQSCELSTCRQQLELIRLQMEQMQLQNGAICHHPAAFGPSLPILEPAQWISILNSNE
                     HLLKEKELLIDKQRKHISQLEQKVRESELQVHSALLGRPAPFGDVCLLRLQELQRENT
                     FLRAQFAQKTEALSREKIDLEKKLSASEVEVQLIRESLKVALQKHSEEVKKQEERVKG
                     RDKHINNLKKKCQKESEQNREKQQRIETLERYLADLPTLEDHQKQSQQLKDSELKSTE
                     LQEKVTELESLLEETQAICREKEIQLESLRQREAEFSSAGHSLQDKQSVEETSGEGPE
                     VEMESWQKRYDSLQKIVEKQQQKMDQLRSQVQSLEQEVAQEEGTSQALREEAQRRDSA
                     LQQLRTAVKELSVQNQDLIEKNLTLQEHLRQAQPGSPPSPDTAQLALELHQELASCLQ
                     DLQAVCSIVTQRAQGHDPNLSLLLGIHSQHPETQLDLQKPDVIKRKLEEVQQLRRDIE
                     DLRTTMSDRYAQDMGENCVTQ"
     exon            189..341
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            342..1036
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1170
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1288
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1474
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1627
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1782
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1876
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1927
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2035
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2261
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..3934
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3915..3920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /note="hexamer: AATAAA"
     polyA_site      3934
                     /gene="CEP85"
                     /gene_synonym="CCDC21"
                     /note="major polyA site"
ORIGIN      
        1 gtgcaacggc cgttagagga gctgagggag ggaaccaccg ctcaccgcag acgtggtggc
       61 tgcagtcagt cttcccgagt gagggatttc gccgcccgct ttcaggcccg tttggcttaa
      121 ataactgtga ttgatggcca tgcaggagaa atatccaact gaggggatct ctcacgtcac
      181 ttcaccgagt tccgatgtga ttcagaaggg cagttccctg gggactgaat ggcagacccc
      241 agttatctcg gagccctttc ggagccgctt cagccgctgt tcaagtgtag ccgacagtgg
      301 ggacacagcc attggtacat catgctcaga tattgcggag gatttttgca gctcaagtgg
      361 cagtcctcct ttccagccca tcaaaagcca cgtaaccatt ccaacagccc atgtgatgcc
      421 ttctacttta gggacctctc ctgccaagcc aaattctaca cctgttggac cctcttcctc
      481 taaactccct ttgtcagggt tggctgaaag tgtgggaatg acaagaaatg gagacctcgg
      541 tgcaatgaaa cattctccag gcctatctag agatctcatg tatttctctg gtgctactgg
      601 agaaaatgga attgagcagt cctggtttcc agcagtgggc catgaaagac aagaagaggc
      661 gaggaagttt gatattccta gcatggaatc taccctcaat cagtcggcaa tgatggagac
      721 actttattca gatcctcacc accgagtccg cttccacaac ccaagaacca gcacaagtaa
      781 ggagttgtac agagtgttgc ctgaggccaa gaaggcaccg ggcagcgggg cagtgtttga
      841 gcggaatgga ccacattcta atagcagtgg ggtcctccct ttgggactcc agcctgctcc
      901 cgggctctcc aagcctctgc cctctcaggt gtggcagccg agtcctgaca cttggcatcc
      961 ccgagagcaa tcttgtgaac tcagcacttg tcggcagcag ctggaattga ttcgtttaca
     1021 gatggagcaa atgcagcttc agaatggagc catctgccac catcctgctg cttttggtcc
     1081 ttcactgccc atcttagagc cagcacagtg gatcagcatc ttgaacagta atgaacacct
     1141 tctgaaggaa aaagagcttc tcattgacaa gcagaggaaa cacatctctc agctggagca
     1201 gaaagtgcga gagagcgaac tgcaagtcca cagtgccctc ttgggccgcc ctgccccctt
     1261 tggtgatgtc tgcttgctga ggctacagga attgcagcga gaaaacactt tcttacgtgc
     1321 acagtttgca cagaagacag aagccttgag cagagaaaag attgaccttg aaaagaaact
     1381 ctctgcttct gaagttgaag tccagctcat cagagagtcg ctcaaagtgg cgttgcagaa
     1441 gcattctgag gaagtgaaga aacaggaaga aagggtcaaa ggtcgtgata aacatatcaa
     1501 taatttgaaa aagaaatgcc agaaggaatc agagcagaac cgggagaagc agcagcgtat
     1561 tgagaccttg gagcgctacc tggctgacct gcccacactg gaagaccatc agaagcagag
     1621 ccagcagctt aaggattctg agttgaagag cacagagctg caggagaaag tgactgagct
     1681 ggagagtttg ctggaggaga cccaggcaat ctgcagagag aaggagattc aactggaaag
     1741 cctgaggcag agagaagcag aattctcctc cgctggacat agcctgcaag ataaacagtc
     1801 tgtggaggag accagtggag aaggtccaga agtggaaatg gagtcctggc agaagcgata
     1861 cgattcgctc caaaagattg tggagaagca gcagcagaag atggatcagt tgcgctcaca
     1921 agtacagagc ctagagcagg aagtggctca agaagaagga acaagccagg ccctgagaga
     1981 ggaggcccag cgaagggatt cagccctgca gcagctgcgc acagccgtga aggagctttc
     2041 agtgcaaaac caggacttga ttgagaagaa tctgacactc caggaacacc tgcgccaggc
     2101 ccaaccaggg tctccacctt caccagacac ggcccagctg gcacttgagc tgcaccagga
     2161 gttggccagt tgccttcaag atctgcaggc tgtctgtagc attgtgaccc agagggccca
     2221 gggccatgac cccaatctct ccctgctcct gggcattcac tcacagcacc cagagactca
     2281 gctagatttg cagaagccag atgtgatcaa gaggaaacta gaagaggttc aacagctgcg
     2341 tcgtgacatt gaggacttaa ggaccaccat gtcagacaga tatgcccagg acatgggaga
     2401 aaactgtgtc acacagtgag gaattctggg ggattccccc agggaggagc tgggctgctg
     2461 agagcctagt ccagcaggtt tctgccctga cattctcttg tctgctattc ccagagaggt
     2521 ctcagagggg aggggagagc ctgcatctgg gggccaaggg ctgattaggg aactgtgtcc
     2581 tacccacact ggcatgttgg attacgtttg tcctgttaat tcactctaga cggtgagtta
     2641 ctaattaact tttggcaggt acaacagata agtcctcaca aactgttccc agccctaggc
     2701 tgacatgaga gacgaacagg ctgtccacct ttcttctcca tccaatggga tggatccatg
     2761 ttcattccca cttcacacct tggtcctgct gattagagag ctcatcagag gggcctggaa
     2821 aggctgagca agtaccagtg acaatggcca tttaagaatt ctcaggcccc atgtgccagc
     2881 cttcttggga actgagctgg ctttctgggt tttctcatgc ctggtcttac tgcttcttcc
     2941 tcagggctct tgttctccca gaagcctcag ggtaatgtgt tggttagcac gtaactacta
     3001 ggattggggc cctagggatt atagccagga ctctaatctg cctaccatgc catttaacaa
     3061 gagatcccac tctccagctg ccttgtgtcc ctagggtcct ggccatgtgt ttagtgtgct
     3121 aaactttctc ctttgttctc aggccttcca ggtagtcccc ttcctggact taagagtgca
     3181 aactcttctc tgtggttcta gccttgggca gaattatatc ccagagacca cagagcaact
     3241 gtcaagctgc ttaccccctc acccagggct acagcctgtg cccagccctc taatttgtgc
     3301 ctctcttgtg ttgggggtgg tgggggttat tcctttccct ttcctgctct ggcctccttg
     3361 aaagttcaga gtacccagta caagtcagct ttaaagtaca gcttttagtg tttcctgggt
     3421 tgtttctctg gggctttagt gagggacctt tgccctctgg tttttcttgc ctcctggttt
     3481 agggagcatc tcacacttgt tagtatctgg ttgttgggcc agccgtgcct cctctagatc
     3541 tggagccagg ccaggcaggg gccacgtgtg ggccagtcag ccactacaag atttttgcta
     3601 agctttgggc tgttggcagc atcttggacc tcatgcctgg gcctgaatga ggctctttct
     3661 taagtgtttt tacaagtttg tgttttattt atggagtgac ttatcccttc cattcagagc
     3721 agccccaccc agccagccct tcagcctctg ggctcctgct gcttaaaggc agagccgcct
     3781 ggttggtctc caccctgtgc attgggagcc cagccaccat gctgacgggt atttttcctc
     3841 ataaagttta taaccagtta tttatatgaa tctttgttat gtccatttgt ttgtattgcg
     3901 tattttgatt ataaaataaa gtatcttaac agac
//
