LOCUS       NM_001349840           11188 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens centrosomal protein 78 (CEP78), transcript variant 8,
            mRNA.
ACCESSION   NM_001349840
VERSION     NM_001349840.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Zhu,T., Zhang,Y., Sheng,X., Zhang,X., Chen,Y., Zhu,H., Guo,Y.,
            Qi,Y., Zhao,Y., Zhou,Q., Chen,X., Guo,X. and Zhao,C.
  TITLE     Absence of CEP78 causes photoreceptor and sperm flagella
            impairments in mice and a human individual
  JOURNAL   Elife 12, e76157 (2023)
   PUBMED   36756949
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   Zhang,X., Zheng,R., Liang,C., Liu,H., Zhang,X., Ma,Y., Liu,M.,
            Zhang,W., Yang,Y., Liu,M., Jiang,C., Ren,Q., Wang,Y., Chen,S.,
            Yang,Y. and Shen,Y.
  TITLE     Loss-of-function mutations in CEP78 cause male infertility in
            humans and mice
  JOURNAL   Sci Adv 8 (40), eabn0968 (2022)
   PUBMED   36206347
  REMARK    GeneRIF: Loss-of-function mutations in CEP78 cause male infertility
            in humans and mice.
REFERENCE   3  (bases 1 to 11188)
  AUTHORS   Lahteenoja,L., Hakli,S., Tuupanen,S., Kuismin,O., Palosaari,T.,
            Rahikkala,E. and Falck,A.
  TITLE     A novel frameshift variant in CEP78 associated with nonsyndromic
            retinitis pigmentosa, and a review of CEP78-related phenotypes
  JOURNAL   Ophthalmic Genet 43 (2), 152-158 (2022)
   PUBMED   35240912
  REMARK    GeneRIF: A novel frameshift variant in CEP78 associated with
            nonsyndromic retinitis pigmentosa, and a review of CEP78-related
            phenotypes.
            Review article
REFERENCE   4  (bases 1 to 11188)
  AUTHORS   Igelman,A.D., Ku,C., da Palma,M.M., Georgiou,M., Schiff,E.R.,
            Lam,B.L., Sankila,E.M., Ahn,J., Pyers,L., Vincent,A., Ferraz
            Sallum,J.M., Zein,W.M., Oh,J.K., Maldonado,R.S., Ryu,J.,
            Tsang,S.H., Gorin,M.B., Webster,A.R., Michaelides,M., Yang,P. and
            Pennesi,M.E.
  TITLE     Expanding the clinical phenotype in patients with disease causing
            variants associated with atypical Usher syndrome
  JOURNAL   Ophthalmic Genet 42 (6), 664-673 (2021)
   PUBMED   34223797
  REMARK    GeneRIF: Expanding the clinical phenotype in patients with disease
            causing variants associated with atypical Usher syndrome.
REFERENCE   5  (bases 1 to 11188)
  AUTHORS   Goncalves,A.B., Hasselbalch,S.K., Joensen,B.B., Patzke,S.,
            Martens,P., Ohlsen,S.K., Quinodoz,M., Nikopoulos,K., Suleiman,R.,
            Damso Jeppesen,M.P., Weiss,C., Christensen,S.T., Rivolta,C.,
            Andersen,J.S., Farinelli,P. and Pedersen,L.B.
  TITLE     CEP78 functions downstream of CEP350 to control biogenesis of
            primary cilia by negatively regulating CP110 levels
  JOURNAL   Elife 10, e63731 (2021)
   PUBMED   34259627
  REMARK    GeneRIF: CEP78 functions downstream of CEP350 to control biogenesis
            of primary cilia by negatively regulating CP110 levels.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11188)
  AUTHORS   Nikopoulos,K., Farinelli,P., Giangreco,B., Tsika,C.,
            Royer-Bertrand,B., Mbefo,M.K., Bedoni,N., Kjellstrom,U., El
            Zaoui,I., Di Gioia,S.A., Balzano,S., Cisarova,K., Messina,A.,
            Decembrini,S., Plainis,S., Blazaki,S.V., Khan,M.I., Micheal,S.,
            Boldt,K., Ueffing,M., Moulin,A.P., Cremers,F.P.M., Roepman,R.,
            Arsenijevic,Y., Tsilimbaris,M.K., Andreasson,S. and Rivolta,C.
  TITLE     Mutations in CEP78 Cause Cone-Rod Dystrophy and Hearing Loss
            Associated with Primary-Cilia Defects
  JOURNAL   Am J Hum Genet 99 (3), 770-776 (2016)
   PUBMED   27588451
  REMARK    GeneRIF: data strongly suggest that mutations in CEP78 cause a
            previously undescribed clinical entity of a ciliary nature
            characterized by blindness and deafness but clearly distinct from
            Usher syndrome, a condition for which visual impairment is due to
            retinitis pigmentosa
REFERENCE   7  (bases 1 to 11188)
  AUTHORS   Brunk,K., Zhu,M., Barenz,F., Kratz,A.S., Haselmann-Weiss,U.,
            Antony,C. and Hoffmann,I.
  TITLE     Cep78 is a new centriolar protein involved in Plk4-induced
            centriole overduplication
  JOURNAL   J Cell Sci 129 (14), 2713-2718 (2016)
   PUBMED   27246242
  REMARK    GeneRIF: the interaction between Cep78 and the N-terminal catalytic
            domain of Plk4 is a new and important element in the centrosome
            overduplication process.
REFERENCE   8  (bases 1 to 11188)
  AUTHORS   Zhang,M., Duan,T., Wang,L., Tang,J., Luo,R., Zhang,R. and Kang,T.
  TITLE     Low expression of centrosomal protein 78 (CEP78) is associated with
            poor prognosis of colorectal cancer patients
  JOURNAL   Chin J Cancer 35 (1), 62 (2016)
   PUBMED   27357513
  REMARK    GeneRIF: CEP78 functions as a tumor suppressor in colorectal cancer
            and low CEP78 expression leads to shorter survival in colorectal
            cancer patients.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 11188)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   10 (bases 1 to 11188)
  AUTHORS   Andersen,J.S., Wilkinson,C.J., Mayor,T., Mortensen,P., Nigg,E.A.
            and Mann,M.
  TITLE     Proteomic characterization of the human centrosome by protein
            correlation profiling
  JOURNAL   Nature 426 (6966), 570-574 (2003)
   PUBMED   14654843
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353705.23 and CB241629.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001349840.1.
            
            Summary: This gene encodes a centrosomal protein that is both
            required for the regulation of centrosome-related events during the
            cell cycle, and required for ciliogenesis. The encoded protein has
            an N-terminal leucine-rich repeat (LRR) domain with six consecutive
            LRR repeats, and a C-terminal coiled-coil domain. It interacts with
            the N-terminal catalytic domain of polo-like kinase 4 (PLK4) and
            colocalizes with PLK4 to the distal end of the centriole. Naturally
            occurring mutations in this gene cause defects in primary cilia
            that result in retinal degeneration and sensorineural hearing loss
            which are associated with cone-rod degeneration disease as well as
            Usher syndrome. Low expression of this gene is associated with poor
            prognosis of colorectal cancer patients. [provided by RefSeq, Mar
            2017].
            
            Transcript Variant: This variant (8) uses an alternate exon
            structure in the 3' coding region, and differs in the 3' UTR,
            compared to variant 1. The encoded isoform (h) is shorter and has a
            distinct C-terminus, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.9741.1,
                                           SRR1660807.171719.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-529               AL353705.23        110618-111146
            530-702             AL353705.23        114566-114738
            703-775             AL353705.23        114835-114907
            776-879             AL353705.23        116239-116342
            880-1054            AL353705.23        118005-118179
            1055-1168           AL353705.23        121212-121325
            1169-1233           AL353705.23        122834-122898
            1234-1345           AL353705.23        123305-123416
            1346-1484           AL353705.23        126448-126586
            1485-1530           AL353705.23        127775-127820
            1531-1659           AL353705.23        129379-129507
            1660-1737           AL353705.23        137450-137527
            1738-1904           AL353705.23        138693-138859
            1905-2076           AL353705.23        139915-140086
            2077-2338           AL353705.23        140985-141246
            2339-9934           AL353705.23        145384-152979
            9935-9935           CB241629.1         18-18               c
            9936-11188          AL353705.23        152981-154233
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q21.2"
     gene            1..11188
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="centrosomal protein 78"
                     /db_xref="GeneID:84131"
                     /db_xref="HGNC:HGNC:25740"
                     /db_xref="MIM:617110"
     exon            1..529
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     CDS             277..2349
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="isoform h is encoded by transcript variant 8;
                     centrosomal protein of 78 kDa; centrosomal protein 78kDa"
                     /codon_start=1
                     /product="centrosomal protein of 78 kDa isoform h"
                     /protein_id="NP_001336769.1"
                     /db_xref="GeneID:84131"
                     /db_xref="HGNC:HGNC:25740"
                     /db_xref="MIM:617110"
                     /translation="MIDSVKLRRDSAADFFSHYEYLCALQNSVPLPAVRACLREGVLD
                     FNADRLRGVDWAPLLSTLKINKDLPLVSIKSFFQPWLGDTGSDMNKFCRSRVPAIRYK
                     DVTFQLCKALKGCLSISSVLKNLELNGLILRERDLTILAKGLNKSASLVHLSLANCPI
                     GDGGLEIICQGIKSSITLKTVNFTGCNLTWQGADHMAKILKYQTMRRHEETWAESLRY
                     RRPDLDCMAGLRRITLNCNTLIGDLGACAFADSLSEDLWLRALDLQQCGLTNEGAKAL
                     LEALETNTTLVVLDIRKNPLIDHSMMKAVIKKVLQNGRSAKSEYQWITSPSVKEPSKT
                     AKQKRRTIILGSGHKGKATIRIVGLATKKPVSSGRKHSLGKEYYAPAPLPPGVSGFLP
                     WRTAERAKRHRGFPLIKTRDICNQLQQPGFPVTVTVESPSSSEVEEVDDSSESVHEVP
                     EKTSIEQEALQEKLEECLKQLKEERVIRLKVDKRVSELEHENAQLRNINFSLSEALHA
                     QSLTNMILDDEGVLGSIENSFQKFHAFLDLLKDAGLGQLATMAGIDQSDFQLLGHPQM
                     TSTVSNPPKEEKKALEDEKPEPKQNALGQMQNIQFQKITGDARIPLPLDSFPVPVSTP
                     EGLGTSSNNLGVPATEQRQESFEGFIARMCSPSPDATSGTGSQRKEEELSRNSRSSSE
                     KKTKTESH"
     exon            530..702
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            703..775
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            776..879
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1054
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1168
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1233
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1345
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1484
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1530
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1659
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..1737
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1904
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2076
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2338
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..11188
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3162..3167
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="hexamer: AATGAA"
     polyA_site      3182
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="major polyA site"
     regulatory      9905..9910
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="hexamer: AATGAA"
     polyA_site      9929
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="major polyA site"
     polyA_site      9993
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
     regulatory      10048..10053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="hexamer: AAGAAA"
     polyA_site      10070
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
     regulatory      11164..11169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
                     /note="hexamer: AATAAA"
     polyA_site      11188
                     /gene="CEP78"
                     /gene_synonym="C9orf81; CRDHL; IP63"
ORIGIN      
        1 ggcttgaatc ccggcgcctc agaggactat gaggcgggcg ccaactgctt gggccgcagg
       61 gcgggaggca gcgcgggagt ggggcgttga ggggccggcc tagcttgggg ctctggcctt
      121 gcgtcttccg accgaatcac cgctcctgag cccggtgcgg ggctgccgct atcgcctggc
      181 cgtgggtgcc ggagcggccg ggttgcgact cagcgttctt gggtgggcgc gggcggcgtc
      241 tccgcggcgg gcatcccccg aggccgccct cgggccatga tcgactccgt gaagctgcgc
      301 cgcgacagcg cggcggactt cttctcccac tacgagtacc tgtgcgcgct gcagaactcg
      361 gtgccgctgc ccgccgtgcg cgcctgtctc cgggagggcg tgctggattt caacgccgac
      421 cgcctccgcg gggtggactg ggcgcctctg ctgagcaccc tcaagatcaa taaagacctg
      481 cccttggtct ccatcaagag cttcttccag ccctggctgg gggacacagg ttctgacatg
      541 aataaatttt gcagaagtcg tgttcctgcg ataagataca aagatgtgac cttccagttg
      601 tgtaaagctc ttaaaggctg tttaagtata tcaagtgtgc taaagaacct ggagctaaat
      661 ggactaattc tgagagagag ggatttaact attctagcaa agggattgaa taaatcggct
      721 tctttggtgc acctgtctct tgcaaattgt ccaattggag atggaggttt agaaattatt
      781 tgtcaaggta taaagagctc tatcactctt aagacagtca acttcacagg atgtaatctg
      841 acatggcagg gagcagatca catggccaag atcttaaagt atcagaccat gagaaggcat
      901 gaagaaacct gggctgagag tcttcgctat aggagacctg atcttgactg tatggctggc
      961 ttaagacgta tcacactgaa ttgcaacaca cttattggtg acctaggtgc atgtgctttt
     1021 gcagactctc tcagtgagga tttatggctg agagctcttg acctgcaaca gtgcggcctc
     1081 accaatgaag gagcaaaggc tttgctagag gcccttgaaa ccaatacaac tctggtcgtt
     1141 ctggatataa gaaaaaatcc actcattgat cattctatga tgaaagcagt tatcaaaaaa
     1201 gtcctccaga atggaaggag tgccaaatca gagtaccagt ggataacttc tccatcagtg
     1261 aaggaaccat ccaaaactgc taaacagaaa aggagaacta taattctagg aagtggtcac
     1321 aaaggaaaag ctactattag aattgtagga ttggctacaa agaaacctgt aagtagtggc
     1381 agaaaacact cccttggtaa agaatattat gcgcccgcac ctcttccacc tggtgtgtct
     1441 ggtttcttgc cgtggcgtac tgcagaacgt gcaaaaagac acaggggttt cccattaatc
     1501 aaaacacgtg atatatgtaa tcagttgcag caaccaggtt ttcctgtgac tgtgacagta
     1561 gagagtcctt catcctctga agttgaagag gttgatgatt cttcagagag tgttcatgaa
     1621 gtgcctgaga aaactagtat agaacaagaa gcattacagg aaaaactgga ggagtgccta
     1681 aagcagttaa aggaagaaag agtgataagg cttaaggttg ataaacgagt cagtgagctg
     1741 gaacatgaaa atgcccagtt aagaaatata aatttctctt tgtctgaagc ccttcatgca
     1801 cagtcattga caaatatgat cctggatgat gaaggtgttt tgggcagcat tgagaattct
     1861 tttcagaagt ttcatgcttt cttggatctc cttaaagatg ctgggcttgg gcagcttgcc
     1921 acaatggctg ggatagatca gtcagatttt caattactag gtcatcccca gatgacttct
     1981 actgttagta atccacctaa agaagaaaag aaggcgcttg aagatgaaaa accagaaccg
     2041 aagcagaatg ccctagggca aatgcaaaat atccagtttc agaaaattac aggtgatgct
     2101 agaattcctt tgcctctcga ctcctttcct gtcccagttt ctactccaga gggcttagga
     2161 acttccagca acaacctagg agtcccagct actgagcagc ggcaggagtc ttttgaagga
     2221 ttcattgcta gaatgtgttc tccttcacca gatgcgactt ctggaactgg aagtcaaaga
     2281 aaagaagagg agttgtccag aaatagcaga tcttcttcag agaaaaagac caaaacagaa
     2341 tcccattgaa atgactggag aaatattaaa ataaaaataa tagcagagtt ggaaaaccag
     2401 aaatttgaac agtgaaattt ctggaagata agaagcagat gatttaagta ccagttaatt
     2461 aaaggatgga acagctaagc cattccactc atctttggag catctgattc tggagtttgc
     2521 caccaggcta agaaagcagc tatctgaagt gggagctctg acccaagaaa tgctgggatc
     2581 ggagaataag ggaattatcc aaaatggctc cgaagaggaa ctgaagttaa gctgcccaca
     2641 tgatctctct aactatgatg acctgccact tccgtttata atcaccatat aagtgcctgt
     2701 aatcatttgt gttcattaaa agtgaaccag aattcccatt tggatgaaaa aataacactt
     2761 ccaactttaa tcttaggccc tcatttataa atatggacaa ccaagaatca tcaaatttga
     2821 agaaaaccag taacataaaa ggaggcatga aattaaaatt aacctgttca agaagatagt
     2881 tactaggaga aacatgaaat ttttaaatta atgaatcaaa atcttcagca attcataaag
     2941 atactgtgtt cataaagaat aggatgccat gaaaaaaata tttagagttt ctggaaatta
     3001 aaaatttgat tatgaaactg aaacactcag aagatggact acacagcaga atggattcat
     3061 ttgaagctta aattcatgaa atggaaggta ttaattggtc ttagacgttt catcagcaac
     3121 ttaattactt agaaaaaatc tttctcagag taactaagca aaatgaacaa tgaacccatt
     3181 aacgtgtggt tttgtttttt gggttttttt tttttgagtc aaggtctcac tcatgtcacc
     3241 caggctgggg tgtagtgatg ggatcacagc ttattgtaac catgaaccat tgggcttaaa
     3301 ttatcttccc acctcagcct cccaagtagc tgcaatgtac caccatgccg aagttttaaa
     3361 aaattattgt agagttgggt tctttctttc ttttcttttt ttcttttttt cttttttttt
     3421 tgagacggag tctcgctcta ttgcccaggc tggagtgcag tggtacaatc tcagctcact
     3481 gcaacctcct cgatctcagc tcactgcaac cgtgaagtga tcttcctgcc tcagcccccc
     3541 tagtaactgg gattacaggc acacaccacc atgcctggct aatttttgta tttttagtaa
     3601 agacggggtt tcaccatgtt ggccaggctg gtctcgaact cctaacctca ggtgattcac
     3661 ccacctcggc ctcccaaagt gctgggatta caggcatgag ccactgcatc cggccaagat
     3721 gggttctttc ttaggctggt cttgaactcc tggcctcaaa tgatcctccc accttggcct
     3781 cccaaagtgc tgagactaca gacctgagcc actgtgccca gccccatgaa agatgtttag
     3841 atcaaataaa ctgataaaga tattgataaa gatatgagac aaactacttc agctttgtga
     3901 cctgcaaatc cccttttata atcaccagag gtgcctgtag tcagaaaaag tctaaataaa
     3961 tactaatgac atatgaaaac aaagtccaac caaagttcta ggtttgaaca tgaatggagg
     4021 catggcagga cacagaaagt gaaagcttgt cctagatatt gtcttattca tgggaaggat
     4081 acaggtagtg tttgattcta ggtattgatt gaaaaaatat attaaaatat atataaagtt
     4141 aagggtatgt tagtaaggat aggttaggtg atgttgcatt aacagctccc aaattttaat
     4201 tactttcaac aacataggtt tatttgtgcc catgcagttt gcctttggca gattggctag
     4261 aggcccctat tcaacatcaa cctcaatctg gaagcgaggc tggtggaacg tcactatttg
     4321 gaacattgct ggttgcagtg tccaaggaaa gacagtatga tgcagcatat attgtgactt
     4381 aaagcttctg tctagatgtg acatatgtca cttctgttca catttctttg gccaaagaaa
     4441 gtcacatggt tatgcctgag ttcagcagac cagggaagtg cagaattgcc atttgccatg
     4501 agaagagagg gaagcaaatg tatgtcaaca actttaatag ctacaaaact acctggaaag
     4561 agggaatgga atttttaggt ccaacaagac gggaaagaaa aagaaacaag ctacatagaa
     4621 ataaaaaatc cacaatatag caagatcaag ttttttactc ataataaaca tgaacaggtt
     4681 aaattctgga atccttaggc ttgagtaaaa acatgaaatc ccactgtgtg ctatttgcaa
     4741 aatctaaaac gctgtcatag attataaaga gatgaacaaa aatataccag gcagatgcta
     4801 aagagtaaat gtggctgtgt tagctgcaga ctaaatagaa cccaaggtga aaattggtaa
     4861 aggggacaaa agcttctttt ggctgataag tacagtgttg cagaagattt tttaaaaaaa
     4921 tatatctgca atttaaaaac ttgatgttag gccgggcgca gcggctcacg cctgtaatcc
     4981 tagcactttg ggaggccgag gtgggtggat cacctgaggt cagaagttca agaccagcct
     5041 ggccaacatg gcaaaactcc gtctctacta aaaatacaaa aaaaaaaaaa aaaaattagc
     5101 tgggtgtcat ggcgcatgcc tgtaatccca gctactcggg aggctgaggc aggagaatcg
     5161 cttgaacctg ggaggcggag tttgcagtga gctgagatgg caccgctgca ctccagcctg
     5221 ggccatagag gagactccgt ctcaaacaaa caaaaacctt catcttaaaa ttatagggta
     5281 agaatttaga aatacaagga aaaatggcca gccagttata acaagaagct ttaagacatc
     5341 tcttttcaga atcagtggat cgactgctgc acacacacta gaatggctaa cattttgaaa
     5401 tgctgataaa tatgcagagc aactggagtt cctcacagct ggtaggaatg caaaatgact
     5461 cagtcactca aaacagtttg gcagattctt taaatgctaa catacacata ccatatgacc
     5521 aagcagtccc actcctgggt atttagagag atgaaaactt agttcacaca aaacctgtgc
     5581 gtggatattt atagcagctc cattcatagt gaccaagatg tcttccaaca ggtgaatgga
     5641 tagcgatgta tccatatggt ggcaaacatg ccacaattcg gatgaatctc aacatcatat
     5701 gctgagtgaa aaagccagtc tcggaaggct acattctgta tgattccatt tatatggcat
     5761 tcttgaaaag acaaaactat agtgacagaa cagattggag attgccaggt gttggggttg
     5821 ggaggagttg attacaaaga ggctgtacaa gggagtttgt ttgtttgttt gttttggtga
     5881 tgaacagttc tgtatcctga ttgcggtggt ggtaacgtga gtctatacat atgttaaaat
     5941 ttatagaact gcatactctc aaaaaaatta gttttactct ataataatat tagagcttaa
     6001 aaaattcatc cctcttgccc atcagtagat caggatatga aggataccat tgaacataaa
     6061 tattttgtat ccatgatgaa tacaaagtat attctcctgg aaaaccaata gaacattcat
     6121 ataaatgatt cctatgaagg taaaaaactt acaaaattca aagatcatac agatcatgtg
     6181 ctctgtataa tgtaataata gtaacaaaag gcctgtccac ttggaaattt ttaaatgatc
     6241 ttctaaataa ctcatttaaa ggagaaatca aaataaattg caaattattt agaattaata
     6301 aaaacttctc taaagctgag gaattctacc aaagaggtgt tagaggaaat tgtatagatt
     6361 ttgatttact ttccaggaag aaagaagaca aaagagatga attaaacatt taaagctaag
     6421 aaagagataa accaaaagaa aggtgaggaa ttggtaaaag cagaaatgaa taaaatagca
     6481 aattttgatc agtgaaacac aggtttttaa aaatgaaaga taaacctttg taaaatatga
     6541 taaagaaaaa aaagcaagaa gtaattaaca aaaattgcca gcattaggaa taattttaga
     6601 aggataccta caactagaag agatattttt aattatgagc acttttataa tttactataa
     6661 ataattgtgt agtgttaaac caataaattt gaaagactag atgaaatgtg actgtttaaa
     6721 aatatggatt gtaaaaattg ataaaaaata gaaaatgtaa ataaacgaaa catcttagga
     6781 gaaattgaaa aaataggaca agaacttatt tttaaaaaga ggccaggtgc ggtggcccat
     6841 gcctgtaata ccagtacttt gggaggccga ggtgggcgga tcacctgaga tgaggagttc
     6901 aagaccagcc tggccaacat ggtgaaaccc cgtctctact aaaaatacaa aaattagccg
     6961 ggcttggtgg cgggcacttg taatcccaac tactcaggag tctgaggcat gagtgtcgct
     7021 tgaacctggg aggcagaggt tgcagtgagc tgaggtcaca ccgttgcact ctagcctggg
     7081 agacaagagg gaaactctgt ctccaaaaaa aaaaaaaaaa aaatacagtt agaaaaactt
     7141 taaaggggcc aggtgtggtg gctcatgtct gtaatcccag cactttggga caccaaagtg
     7201 ggaacattgc tgagaccagc agtttgagac cagcctgggc aagacctcat ctctacaaaa
     7261 attaaaaaac aaaacaaaac aaaaaaaccc aaaacttagc caggcatggt ggtgcgtgca
     7321 tgtagtccca gccactcagg aggctgaagt gggaggatcg cttgaggccg ggattcgagg
     7381 ctgcagtgag ctgtgatcat gccaccactg ctctctagcc tgggcaagag tgagactccg
     7441 actcaagaag agaaaaagaa aaaccttcca ggggcacatt tatttgtaaa ccattccaga
     7501 ggatagaaaa gagatgtaag gctccctaat tcattccata cggttagcgt aatccttata
     7561 gcaaactgca caaataaaac acaaggaaaa ctaaaccaaa ttcaattaat gtaggtgcaa
     7621 aaaatccaaa ataaaactag cagtttgaat tcagcattgt agcaaaagat atatcatttt
     7681 caaggaagat ttgtaccaaa acatgcaaga ttgagtgaag agtaactcac tgtagctcac
     7741 taaattaaca gattaaagaa gtatagtcat cttcacaagc tgaaagagta tgtgataaat
     7801 tcaacatcct ctggtattct taacaatctc gaaatagaag gaaacttcct tagcttagct
     7861 tcttggcttc attaaatata cctaacagaa atccacagga aacaaacttg gtgaaaagtt
     7921 tttgctaaga taaaaacggt actaaaaagt acacattttg ggaaatgaga caaggctctc
     7981 gttatttgta aatatggggt cttttttttt ttggacaaaa aaccccctgt gatgatctac
     8041 tggcatgcta ttacagctaa taaagctgaa taaggaggct gtgcacaaca ccaacacacc
     8101 ggtatgaaaa gtgtgtacac cagcaataac caattagaaa atgtatgagg aaatagactc
     8161 tcatccacaa tagctgcgag aagtaaatat tatctagaaa taagattaac aagagaaatg
     8221 taaggcctat ataaataaaa ggataaatct atgctagagg gtataaaaga aacacaaaaa
     8281 tagaggtatg ccatgtataa gacccaacat aataaatagt tctaccaaat taatttataa
     8341 attcaaagca atttcatata accacagtaa agctttttaa atggatgtaa taacctgatt
     8401 ataacattaa tctgaaagtc atcaggcata aagcaatttt gattacatct caaaacaatc
     8461 catggagtta gggtaaatgt gctgttcaca caggaataat cacaggtata gaaccgagaa
     8521 cctaggaaca gagcaatgta tatatatatg tatttaaaaa atggcatcac aagtcagtag
     8581 agaagggttt gaactattca gtaaatgatg ttgaggttat ttactacttt ttcttatttc
     8641 atatcatgga tgaaaaaaat caagatgaat taaacatgga aattaaaaat aactagaaga
     8701 aaatactgga aaaaaattgt aataaatgtg taagaaaggc attcccaacc aagacacaaa
     8761 acccagtagc catgaaaaaa aagatagatt tgactacatt tttaaaaagt aaaacctttc
     8821 actttttaca aaactttgat aaaattagat ttcataaata aaggagtggg aaaatacatg
     8881 taatagaaca gaagatttaa gataatataa agagcaccta caaacaacaa aaattggaca
     8941 aaggctataa aaatatttta taaataagaa atacagacta taaacataaa ctttcaacct
     9001 cgtaagcaat caggaaaatt aaagttaaaa tgagatacta tttttcaata cattgaaatg
     9061 gcagattatt aaagattggt gatagtgctg gagataatgt gaggaaagga gtattctcag
     9121 tcattgtaga aattgataca gcttttggat ggacagtttg acagtgtgta tcaacatgtg
     9181 aaatgtctcc agcctttgat ctaatgacta attttaggca tccagcctga agaaatattc
     9241 acacaagtgc ccaaagagtc agatacaaga ctgtgtgaag tgttttttga gataataaaa
     9301 aactagaaac agcataaatg tacagaaatg cagaaattgt tgaattagta cataccaatt
     9361 tctgacatat aacactctgc agcagataaa gacaatagct ctctagtgtg gacatgaaaa
     9421 tatttctaag acattgacag gacgaagaag ttgcacagta accacgcact cctgtttaca
     9481 tttgtatagt taaaacttgt ttacctatac gtagcatata tgcgtgtgtg caccctagaa
     9541 aagagtctgt gataacacat atcaagctat tgacagcggt tttgtctgct gaaggaggaa
     9601 aaaagggggg cttctcacat tttactccat ttaattctag gttgtttggt ttaaaaaaaa
     9661 agtttttatg tacaacagac aatttttaaa attccttttc atcatagaaa atgaaaatgt
     9721 gtactctgtt atcctttacg ctctgtccat ttttttcttc aaaaatgtgt ctgacctatt
     9781 atcaatgtga atgaaaatga gctttgctac ataacctctt ttcagagctt tgtattcctg
     9841 aagtgttgga gtcgcctaca tggcatagtc aaatgttttt aacttaacca caacccacag
     9901 taaaaatgaa gctttctgca aagttaaaag tagtagtcct ggccccacct tccttgtgtg
     9961 cacgtataat taatggaaaa ttgcatcaaa caactctgat tgtacttcta gtgactaatt
    10021 catgttctgt tctattcctt gcctttaaag aaaattatgt tggcaactca cgaagtatgt
    10081 ttcacaatcc actaatgggt aatgtcctac tgtttggaaa cactggctag agtgtttcaa
    10141 acatctgctc tgtttacccc tgacaaaact gctactcgtg gggcaatgca agtctttccc
    10201 acatttgttt tattttatgg ctaaaagtca tttctgcatt tgcaaattac ttccaaagcc
    10261 taccttcctt gagctcttta actctctata gtcttctggt aattttcttt agatttccct
    10321 tttcagaacc cttttgtgac actgaaaacc tgagttttca aactgataca tctacctgag
    10381 aattttcaca tataatttgg tcaaaaagtt aaaaaataag aagactttgc cctgtgactg
    10441 aggtgctcag aaggtatggc tggctgctca caaaggcaca tagtgagtga taggggctga
    10501 aagggctcag agaatgcccg agttctctta gtacagtttg tacattttaa accactgtga
    10561 actgaaaaaa aaaaaaaagg caaccttggt agtttagaaa tagataagga cccacttata
    10621 gattaaataa taattagctt gttccagaga gaccagtgaa gattagttgg tttatttaat
    10681 gtgttagaaa gatggtcctc aaagtccttg aaatgtgaag gcggctttta tctccttaag
    10741 aaaacaagaa aactccacaa aatcttgaaa gctagataat cctagcttaa atgataaaat
    10801 ttgagtagag tttgaaaaca ctaaaatcag tttgccaaat aaccagctct aatttcttta
    10861 taccaaagcc aaacttcacc taaatcttac ctaagaattg agttccttat tgtaaccaga
    10921 taggagacac ttttgtatat tattccaaat attgcaaacc tacaaggagt aaaaggtgtt
    10981 attattttaa agaagaagga atgaggcctg agaggtattg attcacttgg ttgagggctt
    11041 caagtagcac aaggcaaaag caggatttgt tgacttccaa acctgttttc ttccttcacc
    11101 atgtggcctt tgcatctttc ccacacaaat attattccac aaatatttat tgtcaccaac
    11161 tataataaaa gaacagaact atgtatca
//
