LOCUS       NM_033395               8014 bp    mRNA    linear   PRI 24-MAR-2024
DEFINITION  Homo sapiens centrosomal protein 295 (CEP295), mRNA.
ACCESSION   NM_033395 XR_110903
VERSION     NM_033395.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Li,N., Xu,Y., Chen,H., Lin,J., AlAbdi,L., Bekheirnia,M.R., Li,G.,
            Gofin,Y., Bekheirnia,N., Faqeih,E., Chen,L., Chang,G., Tang,J.,
            Yao,R., Yu,T., Wang,X., Fu,W., Fu,Q., Shen,Y., Alkuraya,F.S.,
            Machol,K. and Wang,J.
  TITLE     Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting
            with primary microcephaly, developmental delay, intellectual
            disability, short stature, craniofacial and digital abnormalities
  JOURNAL   EBioMedicine 99, 104940 (2024)
   PUBMED   38154379
  REMARK    GeneRIF: Bi-allelic variants in CEP295 cause Seckel-like syndrome
            presenting with primary microcephaly, developmental delay,
            intellectual disability, short stature, craniofacial and digital
            abnormalities.
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Zhou,J., Chen,C., Zhao,X., Jiang,T., Jiang,Y., Dai,J. and Chen,J.
  TITLE     Coding variants in the PCNT and CEP295 genes contribute to breast
            cancer risk in Chinese women
  JOURNAL   Pathol Res Pract 225, 153581 (2021)
   PUBMED   34418690
  REMARK    GeneRIF: Coding variants in the PCNT and CEP295 genes contribute to
            breast cancer risk in Chinese women.
REFERENCE   3  (bases 1 to 8014)
  AUTHORS   Atorino,E.S., Hata,S., Funaya,C., Neuner,A. and Schiebel,E.
  TITLE     CEP44 ensures the formation of bona fide centriole wall, a
            requirement for the centriole-to-centrosome conversion
  JOURNAL   Nat Commun 11 (1), 903 (2020)
   PUBMED   32060285
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8014)
  AUTHORS   Hearn,T.
  TITLE     ALMS1 and Alstrom syndrome: a recessive form of metabolic,
            neurosensory and cardiac deficits
  JOURNAL   J Mol Med (Berl) 97 (1), 1-17 (2019)
   PUBMED   30421101
  REMARK    Review article
REFERENCE   5  (bases 1 to 8014)
  AUTHORS   Tsuchiya,Y., Yoshiba,S., Gupta,A., Watanabe,K. and Kitagawa,D.
  TITLE     Cep295 is a conserved scaffold protein required for generation of a
            bona fide mother centriole
  JOURNAL   Nat Commun 7, 12567 (2016)
   PUBMED   27562453
  REMARK    GeneRIF: Study shows that the interaction between Cep295 and Cep192
            seems to be crucial for the integrity of centriole structure and
            also for daughter-to-mother centriole conversion.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8014)
  AUTHORS   Chang,C.W., Hsu,W.B., Tsai,J.J., Tang,C.J. and Tang,T.K.
  TITLE     CEP295 interacts with microtubules and is required for centriole
            elongation
  JOURNAL   J Cell Sci 129 (13), 2501-2513 (2016)
   PUBMED   27185865
  REMARK    GeneRIF: our results indicate that CEP295 directly interacts with
            microtubules, and that excess CEP295 could induce the assembly of
            overly long centrioles. Furthermore, exogenous expression of the
            N-terminal domain of CEP295 exerts a dominant-negative effect on
            centriole elongation. Collectively, these findings suggest that
            CEP295 is essential for building the distal half centrioles and for
            post-translational modification of centr
REFERENCE   7  (bases 1 to 8014)
  AUTHORS   Izquierdo,D., Wang,W.J., Uryu,K. and Tsou,M.F.
  TITLE     Stabilization of cartwheel-less centrioles for duplication requires
            CEP295-mediated centriole-to-centrosome conversion
  JOURNAL   Cell Rep 8 (4), 957-965 (2014)
   PUBMED   25131205
  REMARK    GeneRIF: A newborn centriole-enriched protein, KIAA1731/CEP295,
            specifically mediates centriole-to-centrosome conversion but
            dispensable for cartwheel removal.
REFERENCE   8  (bases 1 to 8014)
  AUTHORS   Jakobsen,L., Vanselow,K., Skogs,M., Toyoda,Y., Lundberg,E.,
            Poser,I., Falkenby,L.G., Bennetzen,M., Westendorf,J., Nigg,E.A.,
            Uhlen,M., Hyman,A.A. and Andersen,J.S.
  TITLE     Novel asymmetrically localizing components of human centrosomes
            identified by complementary proteomics methods
  JOURNAL   EMBO J 30 (8), 1520-1535 (2011)
   PUBMED   21399614
REFERENCE   9  (bases 1 to 8014)
  AUTHORS   Knorz,V.J., Spalluto,C., Lessard,M., Purvis,T.L., Adigun,F.F.,
            Collin,G.B., Hanley,N.A., Wilson,D.I. and Hearn,T.
  TITLE     Centriolar association of ALMS1 and likely centrosomal functions of
            the ALMS motif-containing proteins C10orf90 and KIAA1731
  JOURNAL   Mol Biol Cell 21 (21), 3617-3629 (2010)
   PUBMED   20844083
  REMARK    GeneRIF: data suggest centrosomal functions for C10orf90 and
            KIAA1731 and new centriole-related functions for ALMS1.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DW414641.1, AB051518.2, AP003499.2, AA747565.1, AK095859.1 and
            AP001273.4.
            
            On Nov 23, 2018 this sequence version replaced NM_033395.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB051518.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325212.11/ ENSP00000316681.6
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DW414641.1         50-78
            30-550              AB051518.2         1-521
            551-551             AP003499.2         131796-131796
            552-741             AB051518.2         523-712
            742-742             AP003499.2         138531-138531
            743-1614            AB051518.2         714-1585
            1615-1615           AP003499.2         154859-154859
            1616-1652           AA747565.1         119-155
            1653-6167           AB051518.2         1624-6138
            6168-7428           AK095859.1         386-1646
            7429-7429           AP001273.4         1769-1769
            7430-8012           AB051518.2         7399-7981
            8013-8014           AP001273.4         2685-2686
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q21"
     gene            1..8014
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /note="centrosomal protein 295"
                     /db_xref="GeneID:85459"
                     /db_xref="HGNC:HGNC:29366"
                     /db_xref="MIM:617728"
     exon            1..93
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            94..227
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..7925
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /note="centriolar protein CEP295; centrosomal protein
                     295kDa"
                     /codon_start=1
                     /product="centrosomal protein of 295 kDa"
                     /protein_id="NP_203753.1"
                     /db_xref="CCDS:CCDS44708.1"
                     /db_xref="GeneID:85459"
                     /db_xref="HGNC:HGNC:29366"
                     /db_xref="MIM:617728"
                     /translation="MKRKVVNTHKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDI
                     ALQIREDIKQRRNQQFTRLAEELRAEWEESQTQKIQNLEKLYLASLRSMGEGHRQAKE
                     NEPDLDALAQRAAERKRKADLRHKEALKVQKNQKEILLKQKTWHIKARKEALLVEKER
                     SAKITSLPPPPPTLFENIEVKRISAVKTNSSTYHHLHTFVNRETDTKRPDARLAAEEE
                     AKRLEELQKQAAQERMERFEKAHVRGFQAMKKIHLAQNQEKLMKELKQLQQEDLARRR
                     QTVAQMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPTV
                     TNQIQDEELDLSMEQENLGAAEDLPVTEAEICSSETDVPLVMKTQQIPSKVLFKKLLN
                     KIRSQKSLWTIKSMSEDESEMITTVSEIESKAPTVESGTIASKERTLSSGQEQVVESD
                     TLTIESGPLASEDKPLSCGTNSGKEQEINETLPITTVAQSSVLLHPQEAAARIRMSAR
                     QKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQPTGVGIAPASCP
                     VISDEDSHRQMIRNYQHQLLQQNRLHRQSVETARKQLLEYQTMLKGRCPSVSAPSLIT
                     DSVISVPSWKSERPTAISEHWDQGQRLKLSPNKYQPIQPIQTSKLEQDHFQVARQNHF
                     PQRQVETTETLRASDILTNQALESQEHLRQFSQTETQQRDYKLVPKDSETLSRALSHD
                     RQLISQDARKISETFGATTFQSLESQQLFSENSENISYHLTEPSSFVPLVPQHSFSSL
                     PVKVESGKIQEPFSAMSKSTVSTSHSIISQMHDRPLLPSENITAQQGNMKALQEQLDL
                     QKKVLQATQEAQEQLLLCKQKEVEQQTGLSVFLPLVTPDSSALLPSAKADLGRIQESS
                     PTKNNIAVSSDHHVISQLQDKRLSLSQPILSQQNNFKFLQEQLNIQKDSLQARREAQE
                     VLYVHKQSELDRRVCSEQAEPSFPFQVAQHTFTSLPSADTKSGKIQEQHSSKSEKGLV
                     SCQSDIPISQDGSLSFLQQFLPLHDSLKLLQEQLTKQRDTLQARHEAQVELLLHRQRD
                     LGDSKSGLVSSSSSPVVVQHSVASQASAKAEPRRIQELYLSEKENVGPSCHLIIPTFQ
                     DKSLSFPQHSLAQQENLTILQEQSQIQRVILGAKEGTQEFVHTESELEKRISSEQTGT
                     SSSLSQVDESERFQECISIKSDSTIPLSHPKIPRCQERLLRVSQHMLPLQDNLEEHQA
                     WLDTEKEAFHFSQKTQENTSSEQTGSSSFIPQLVQLSFTSLASAESGTILEPLFTESE
                     SKIFSSHLQIPQLQDRLLRISQLIQPQQDNLKALQEQLATQREAIILARQEAREELLL
                     HQSEWEGRISPEQVDTSSLPLVPQHSFASLPLNESERNQEPCSINSDNIVSSGHSEIP
                     TLPDGLLGLSHLVLPQQDNLIALEEHLHAQTDFLPSIEKTQKELVLSKPCKFEEKVSS
                     EHFIQSHHGDLQALQQQLDTQKKAIRSIQEVQEELLLQRLSELEKRVSSEQVCSSSFV
                     SQVPVADSERTQKSFPTKSNDTLPSSHREIPRLQDRLLSLSKPILPQQDNMTAQLDAQ
                     REVMYSYEKPQEELSLNKQRKLNKSESAEHTIPSLFLPKETEHSFIPLPFAEAKPKST
                     CELYSSQNEHAAPPSNPVIPGFQDRLLSFSQSVLTQQDNLGLQKQLDLQREVLHYSQK
                     AQEKLLVQRQTALQQQIQKHEETLKDFFKDSQISKPTVENDLKTQKMGQLRDWFPNTQ
                     DLAGNDQENIRHADRNNSDDNHLASEDTSAKQSGEHLEKDLGRRSSKPPVAKVKCGLD
                     LNQHELSAIQEVESPAIGRTSILGKPGIYEDRDPLRVSISREQSFFGSPLAHDPFSCL
                     QLVGQENVCGDDYDEAVKLKESVVENHAVLSYAVEEEHAYLGPTVKPDDKAKTLSYEP
                     LSSATVSTGSLLSYENTDLSLTDPESFSEHMDDSKQESTTSKEEETNIISSIVPSTQD
                     IYQRQNSSDVHKSLLPAVDETTCGHTHFQQMIDKYINEANLIPEKTDLQELEHIFPNL
                     HHQLFKPLEPHPDFDLSSSSSGISPDNRDFYQRSDSSSESHCATGLSKSTVYFTALRR
                     TSMHSSLNTSPNQQPDTNLAHVGAHSFATENIIGGSEQCFEQLQPEYSSQEESQHADL
                     PSIFSIEARDSSQGMKNQNYPSEEHTEILQNKKKIVHFQLSIGNLSSVYSSSDEANVF
                     DQLNVQHSTPCGSNSSECSTKHQLESRKESMGFEELSKRGVVTMLQSQGLIEDNKNET
                     CRVLDINPQVEETDSRLCVRTVEMGTSIQAPYSLTTQNEKYFENSAETDIPKITKKLS
                     QLGESELFASSGSFSLQSSIPVWETETGHGIMEEPELTLISTTDTSIAEMDFANLTLE
                     EKSENEAKCFFQVSEFLPLVSATEASDYPAVSELSIEKPRTASTETPRRLTPVPGSLQ
                     EAFIKRKKSFMERSHQRQKEIRNKIHVSENSQIKTVKEKPSISSSVSRLKGVNKVRAS
                     FPEDRKTTQALRHQRGLRLYNQLAEVKQQKEEKTKQEAYAQNRARAKEFHKKTLEKLR
                     AKNTC"
     exon            228..428
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            429..553
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            554..647
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            648..743
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            744..884
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1068
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1233
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1455
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1548
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1652
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1790
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1888
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..5393
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            5394..5571
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            5572..5715
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            5716..5868
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            5869..5969
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            5970..6066
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            6067..6315
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            6316..6437
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            6438..6618
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            6619..7280
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            7281..7421
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            7422..7518
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            7519..7686
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            7687..7786
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            7787..7886
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     exon            7887..8014
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7994..7999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /note="hexamer: AATAAA"
     polyA_site      8014
                     /gene="CEP295"
                     /gene_synonym="KIAA1731; SCKL11"
                     /note="major polyA site"
ORIGIN      
        1 gcagaaacgg ctgctgggca gctgatgggc aggagcttac caggctggct tgctctgagc
       61 tgcggttact gtgtccaggc cccgggttct cagaactgtc atacataaag tacacagaaa
      121 tgaagagaaa agtcgtgaat actcacaagc tgagattgag tcctaatgag gaagccttca
      181 ttttgaagga agattatgaa agaaggcgaa aactaagatt gctacaggtt cgagaacaag
      241 aaagagatat cgccttacag ataagagaag acataaaaca gaggagaaat caacaattta
      301 cacgtttggc agaggagcta agggcagaat gggaagaatc acaaactcag aaaatacaga
      361 acttggaaaa actgtatttg gcaagtttaa gaagtatggg agagggacat cgacaggcca
      421 aagaaaatga acctgatttg gatgctttgg cacagcgggc agcagaaagg aaaagaaaag
      481 cagatttgag gcataaagaa gccttgaaag tacagaaaaa tcaaaaagaa atattactga
      541 aacaaaaaac ctggcatata aaagctcgaa aggaagcact gcttgtggaa aaagagagat
      601 cagccaaaat tacaagtctg ccacctcctc ctccaactct ttttgagaac atcgaagtaa
      661 aaagaatttc tgcagtcaaa accaatagtt ctacctacca tcatcttcac acttttgtga
      721 atagagagac agacacaaaa cggccagatg ctcgtttggc tgctgaagag gaagctaaac
      781 gattggaaga actacaaaaa caggcagcac aagagagaat ggaacggttt gaaaaggcac
      841 atgtacgggg attccaagca atgaagaaga tccatttggc tcaaaatcag gagaaactaa
      901 tgaaagaact caaacagcta cagcaagagg acctggcacg taggagacag actgtagcac
      961 aaatgccacc acaactagtt gaacttccat acaaacgcag tgaaatgaaa gaagactggc
     1021 agagagaatt ggaatttgcc tttgaagata tgtacaatgc agacaggaag gtgaaaggga
     1081 atctgattct gcaccttgaa ccagagccct tgcccactgt gactaatcag atccaagatg
     1141 aagagctgga cctttcaatg gaacaagaaa atttgggtgc agctgaagac cttccagtga
     1201 cagaagctga aatatgttct agtgaaacag atgttccctt ggtaatgaag acccaacaga
     1261 ttccttcaaa agttcttttt aaaaaattat taaataagat ccgaagccaa aaatctctct
     1321 ggacaattaa atctatgtct gaggatgaaa gtgaaatgat tacgactgtt agtgaaattg
     1381 agagtaaagc accaacggtt gagtcaggaa caattgccag caaagagaga acgttatcct
     1441 ctgggcagga acaagttgtt gaaagtgata cactaacaat tgagtctgga ccacttgcta
     1501 gtgaagataa accactttcg tgtggtacaa actctggaaa agaacaagaa ataaatgaga
     1561 ctctgcctat cacaactgta gctcagagtt cagttctact tcatcctcaa gaagcagcag
     1621 ccaggattag aatgtcagca aggcagaaac agataatgga aatagaagag cagaagcaaa
     1681 agcaattgga attacttgaa caaattgaac agcagaaatt aagattagaa actgactgct
     1741 tcagggctca gctggaagaa gaaaaaagaa aaaaaactca accgactggg gttggcattg
     1801 ctccagcatc atgccctgta atttctgatg aagatagtca taggcagatg attcgtaact
     1861 atcaacatca gcttttacaa caaaacaggt tacacaggca gtctgttgaa acagccagga
     1921 aacaattact tgaatatcaa actatgttaa aaggaaggtg cccatcggtg tcagctccat
     1981 cattgataac tgattctgtt atatcagtgc catcatggaa atctgagaga ccgactgcta
     2041 tatcagagca ttgggatcaa ggtcagagac tcaagttgag tcctaacaaa taccaaccca
     2101 tacaacctat acagacctcc aaattagaac aagatcattt tcaggtagcg agacaaaatc
     2161 actttccaca aagacaggtg gaaacaacag aaacattacg cgcttcagat attttaacca
     2221 atcaagcttt agaatcacaa gaacatctaa ggcaattctc tcagactgaa acacaacaga
     2281 gagactataa attggtcccc aaagattctg agacactttc aagggctttg tcacatgaca
     2341 ggcagctaat atcacaggat gctagaaaaa tatctgaaac atttggggca acaacttttc
     2401 aaagtttaga atcccaacaa ttgttctcag agaatagtga aaatatatct taccatttaa
     2461 ctgaaccttc ttcatttgta ccactggtac ctcagcattc ttttagttct ctgcctgtta
     2521 aagttgagtc aggaaaaatt caagaaccct tttcagccat gagcaaaagt acagtttcca
     2581 caagccattc tataatcagc caaatgcatg ataggccttt gctgccgtca gagaatatca
     2641 cagcccagca aggtaatatg aaggccctcc aagaacagtt agacctacag aagaaagttc
     2701 ttcaggcaac tcaggaagct caggaacagt tgcttttgtg caaacagaaa gaagtggaac
     2761 agcaaacggg cctctcggta ttccttccct tggtaactcc agattcatct gctttattgc
     2821 cttctgccaa agcagatttg gggagaatcc aggaatcttc accaaccaag aataatattg
     2881 cagtttcctc agaccatcat gtgatctcac aacttcagga taagcgtttg agtctttcac
     2941 agcctatcct atcacagcaa aataatttta aatttctcca agagcagttg aatattcaga
     3001 aggatagcct tcaggctagg cgagaagccc aggaagtatt gtatgtacat aaacagagtg
     3061 aattggatag aagagtatgt tccgaacagg ctgagccctc tttcccattt caggtagctc
     3121 agcatacatt tacttcacta ccatctgctg atacaaaatc tggaaaaata caggagcaac
     3181 attcatctaa gagcgagaaa ggacttgttt catgccaatc tgacatcccc atatctcagg
     3241 atgggtcttt gagtttccta cagcagttcc tacctctaca tgatagtttg aagttgctcc
     3301 aagaacagtt gactaaacag agggatactc ttcaggctag gcatgaagct caggtggaat
     3361 tacttttaca tagacaaaga gatttggggg acagtaagtc tgggctggtg agctcttcat
     3421 cctcaccagt ggttgttcag cattcagttg cttcacaagc ttctgctaaa gctgagccta
     3481 ggagaattca ggagctttat ttatctgaga aggagaatgt aggtccctcc tgtcatttga
     3541 taatcccaac atttcaggat aagtctctta gttttccaca gcatagcctg gcacagcaag
     3601 aaaatttgac aatactccaa gaacagtcac aaatacaaag ggtaatactt ggtgctaaag
     3661 aaggaactca ggaatttgta cacacagaaa gtgaattgga gaaaagaatt tcttctgaac
     3721 agactggcac ctcctcatcc ctttcccagg tggatgaatc tgagagattc caggaatgta
     3781 tatcaatcaa gagtgacagt accattccct taagccatcc taagatccca agatgtcagg
     3841 aaagactttt gagagtttca caacatatgc tacctctaca agataatttg gaggaacacc
     3901 aagcatggct agacactgag aaagaagcct ttcatttcag ccagaaaacc caagaaaata
     3961 catcttctga acaaactggt tcatcttcat tcatacccca gttggtacag ctttcattta
     4021 cttcgttagc ttcagctgag tctggcacaa tcctggaacc tctttttaca gagagtgaaa
     4081 gtaaaatttt ttcaagccac cttcagatcc cacaattgca ggataggctt ttgaggatat
     4141 cgcaacttat ccagcctcaa caagataatt tgaaggcact tcaagaacag ttagctacac
     4201 agagagaagc catcattcta gctagacaag aagctcggga agaattactt ttacatcaga
     4261 gtgaatggga gggaagaata tctcccgagc aggttgacac ctcttcctta cccctagtac
     4321 cacagcattc attcgcctca ttacctctta atgaatctga aagaaaccaa gaaccatgtt
     4381 caattaacag tgataatata gtatcctcag gtcactcaga gataccaaca ttgcctgatg
     4441 ggctgttggg tttatcacat cttgttttac ctcaacaaga taatttgatt gcacttgaag
     4501 aacacttgca tgcacagaca gatttccttc cttctattga gaaaacccag aaagaattgg
     4561 ttttgtcaaa accatgtaaa tttgaggaaa aggtatcttc tgagcatttt atccagtctc
     4621 accatggtga tttgcaggca cttcaacagc agttagatac acagaagaaa gccattcgat
     4681 ctatacagga agtccaagaa gaattgcttt tgcaaagatt aagtgaattg gagaaaaggg
     4741 tatcatctga acaagtttgc tcctcttcat ttgtatccca ggtgcctgtt gctgactctg
     4801 aaagaaccca gaagtctttc ccaaccaaaa gtaatgatac tcttccctca agtcatcgtg
     4861 agattccaag attacaggat agacttttga gtttatcaaa gcctattctg cctcagcaag
     4921 ataatatgac agcacaattg gatgcacaaa gggaagtgat gtattcttat gagaaacccc
     4981 aggaagaact gtctttaaac aaacaaagaa agttgaacaa aagtgaatct gctgagcata
     5041 ctatcccctc tttgtttcta cccaaggaaa cagagcattc gtttattcca ctaccttttg
     5101 cagaagctaa acctaaaagc acttgtgaat tgtattcatc ccagaatgaa catgcagccc
     5161 ccccaagtaa tcctgtgatc ccagggtttc aagatagact tttgagtttt tcacagtctg
     5221 tcttaactca gcaagataac ttgggacttc agaaacagtt ggatctacaa agagaagttc
     5281 tgcattatag ccagaaagcc caggaaaaat tgcttgtaca gagacaaaca gcattgcagc
     5341 agcagataca gaaacatgaa gagactttga aggatttctt taaagacagt cagataagta
     5401 agcccacagt tgaaaatgat ttaaaaaccc agaagatggg gcagctcaga gactggtttc
     5461 ctaatacaca agacctagca ggaaatgatc aagaaaatat taggcatgca gataggaaca
     5521 actctgatga taatcatttg gcttcagaag atactagtgc caagcaaagt ggtgagcatc
     5581 tggagaaaga tctggggaga agatcctcaa agccacctgt agcaaaagtc aaatgtggtt
     5641 tggacttaaa ccagcatgaa cttagtgcta tacaagaagt agagtcacca gcaattggca
     5701 gaacttctat actaggtaaa ccaggtattt atgaagacag agaccccctg cgagtctcaa
     5761 taagccgaga acaaagtttc tttgggagcc cactggccca tgatccgttt agttgtcttc
     5821 aactggttgg ccaagagaat gtctgtggtg atgactatga tgaagcagtt aagctgaagg
     5881 aatctgttgt tgaaaatcat gcagtgttaa gttatgctgt ggaggaagaa catgcatatt
     5941 tgggtccaac tgtgaagcca gatgataagg ctaaaacact gtcttatgaa ccattatctt
     6001 cagcaactgt ttccactggg agccttttaa gttatgaaaa cacagatttg agccttacag
     6061 atccagagtc attttcagag cacatggatg atagcaagca agaatctacc accagtaaag
     6121 aagaggaaac aaatattata agttccatag ttccttcaac acaagatatt tatcagcggc
     6181 agaactcttc agacgttcat aaatctctgt tgcctgcagt ggatgaaact acatgtggtc
     6241 acacacactt tcagcaaatg atagacaagt acattaatga agcaaatttg atacctgaaa
     6301 aaacagattt gcaagaattg gaacacattt ttcctaattt gcatcatcag ctgtttaaac
     6361 ccttagaacc acatccagat tttgacttat catcatcatc ctctgggatt tctccagaca
     6421 acagagactt ttaccagaga tcagattctt catctgaaag ccactgtgct actggattat
     6481 ccaaaagtac agtttatttc acagcactga ggaggaccag catgcattct tctcttaaca
     6541 caagtccgaa tcaacaacct gacactaact tggctcatgt tggagctcac agttttgcta
     6601 cagaaaatat tattggggga tctgaacaat gttttgaaca gcttcagcca gaatattctt
     6661 cacaggagga gagccagcat gctgatctac caagtatttt tagcattgaa gcaagagatt
     6721 cttcccaagg catgaaaaat cagaactatc cctctgaaga acatactgaa atattacaaa
     6781 acaagaaaaa aattgttcat ttccagcttt ctataggaaa cttaagttca gtctacagtt
     6841 catctgatga agctaatgta tttgatcagt taaatgtaca gcatagcact ccatgtggtt
     6901 ctaactctag tgagtgctca acaaaacacc aactagaaag cagaaaggaa agtatgggct
     6961 ttgaagaact atcaaaaaga ggggttgtta caatgttaca aagtcaagga ctcattgaag
     7021 ataataaaaa tgaaacctgt agggttttag acataaatcc acaggtagag gaaactgact
     7081 ctcgattatg tgtaagaaca gtggagatgg gaacttcaat tcaggcacca tattccttaa
     7141 ctactcaaaa tgaaaaatat tttgagaatt cagctgaaac agacattcca aaaatcacca
     7201 aaaaactatc tcaactagga gaatcagagc tttttgcaag ttctggatca ttttcattac
     7261 agagctctat accagtctgg gaaacagaaa ctggccatgg tataatggaa gaaccagaac
     7321 ttactttaat aagcaccact gataccagta ttgctgaaat ggattttgca aatttaaccc
     7381 tagaagagaa gagcgagaat gaagcaaaat gcttctttca ggtgagtgag tttctgcctc
     7441 ttgtatcagc aacagaagcc tcagattatc cagctgtatc agaactttcc atagaaaaac
     7501 caaggacagc atctacagaa acccctcgca ggcttacacc tgtaccaggg agcttacaag
     7561 aagcatttat aaagaggaaa aaatcattta tggagagatc ccaccagagg cagaaagaaa
     7621 taaggaataa aattcatgtc tctgaaaatt ctcaaatcaa aacagttaaa gagaaaccat
     7681 ctataagttc atctgtgagt cgtctaaagg gcgtgaataa agtcagagca tcttttcctg
     7741 aagacagaaa gactacacag gctctaaggc accaaagggg tctaaggtta tacaatcaac
     7801 tagctgaagt gaaacaacaa aaggaagaaa aaacaaaaca agaagcttat gcccaaaaca
     7861 gagcaagggc aaaagaattc cataagaaaa cactagagaa acttcgagcc aaaaatacat
     7921 gctgactttc tagaaatagt gtaaaggttt tttaattgtg tatatgtagc attagacaaa
     7981 attatttaaa gtcaataaat tgttattcga ggaa
//
