LOCUS       NM_024629               2494 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens centromere protein U (CENPU), transcript variant 1,
            mRNA.
ACCESSION   NM_024629
VERSION     NM_024629.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Zhang,X., Wang,M., Zhang,Y., Yang,J. and Duan,W.
  TITLE     Knockdown of CENPU inhibits cervical cancer cell migration and
            stemness through the FOXM1/Wnt/beta-catenin pathway
  JOURNAL   Tissue Cell 81, 102009 (2023)
   PUBMED   36608638
  REMARK    GeneRIF: Knockdown of CENPU inhibits cervical cancer cell migration
            and stemness through the FOXM1/Wnt/beta-catenin pathway.
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Tian,T., Chen,L., Dou,Z., Yang,Z., Gao,X., Yuan,X., Wang,C.,
            Liu,R., Shen,Z., Gui,P., Teng,M., Meng,X., Hill,D.L., Li,L.,
            Zhang,X., Liu,X., Sun,L., Zang,J. and Yao,X.
  TITLE     Structural insights into human CCAN complex assembled onto DNA
  JOURNAL   Cell Discov 8 (1), 90 (2022)
   PUBMED   36085283
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2494)
  AUTHORS   Xu,Y., Zhang,L., Wang,Q. and Zheng,M.
  TITLE     Overexpression of MLF1IP promotes colorectal cancer cell
            proliferation through BRCA1/AKT/p27 signaling pathway
  JOURNAL   Cell Signal 92, 110273 (2022)
   PUBMED   35122991
  REMARK    GeneRIF: Overexpression of MLF1IP promotes colorectal cancer cell
            proliferation through BRCA1/AKT/p27 signaling pathway.
REFERENCE   4  (bases 1 to 2494)
  AUTHORS   Yu,Y., Chen,X., Zhang,W. and Liu,J.
  TITLE     Abnormal Expression of Centromere Protein U Is Associated with
            Hepatocellular Cancer Progression
  JOURNAL   Biomed Res Int 2021, 4051192 (2021)
   PUBMED   34957303
  REMARK    GeneRIF: Abnormal Expression of Centromere Protein U Is Associated
            with Hepatocellular Cancer Progression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2494)
  AUTHORS   Deng,T., Jiang,X., He,Z., Cai,M., Chen,C. and Xu,Z.
  TITLE     Centromere protein U (CENPU) promotes gastric cancer cell
            proliferation and glycolysis by regulating high mobility group box
            2 (HMGB2)
  JOURNAL   Bioengineered 12 (2), 10194-10202 (2021)
   PUBMED   34872447
  REMARK    GeneRIF: Centromere protein U (CENPU) promotes gastric cancer cell
            proliferation and glycolysis by regulating high mobility group box
            2 (HMGB2).
REFERENCE   6  (bases 1 to 2494)
  AUTHORS   Hanissian,S.H., Teng,B., Akbar,U., Janjetovic,Z., Zhou,Q.,
            Duntsch,C. and Robertson,J.H.
  TITLE     Regulation of myeloid leukemia factor-1 interacting protein
            (MLF1IP) expression in glioblastoma
  JOURNAL   Brain Res 1047 (1), 56-64 (2005)
   PUBMED   15893739
  REMARK    GeneRIF: MLF1IP was found in glioblastomas where it was
            co-localized with MLF1 and nestin. Moreover, it was elevated in the
            contralateral brain where no tumor cells occurred, suggesting a
            role in glioma pathogenesis and potentially in other types of
            malignancies.
REFERENCE   7  (bases 1 to 2494)
  AUTHORS   Hanissian,S.H., Akbar,U., Teng,B., Janjetovic,Z., Hoffmann,A.,
            Hitzler,J.K., Iscove,N., Hamre,K., Du,X., Tong,Y., Mukatira,S.,
            Robertson,J.H. and Morris,S.W.
  TITLE     cDNA cloning and characterization of a novel gene encoding the
            MLF1-interacting protein MLF1IP
  JOURNAL   Oncogene 23 (20), 3700-3707 (2004)
   PUBMED   15116101
  REMARK    GeneRIF: a possible role for MLF1IP deregulation in the genesis of
            erythroleukemias
REFERENCE   8  (bases 1 to 2494)
  AUTHORS   Obuse,C., Yang,H., Nozaki,N., Goto,S., Okazaki,T. and Yoda,K.
  TITLE     Proteomics analysis of the centromere complex from HeLa interphase
            cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of
            the CEN-complex, while BMI-1 is transiently co-localized with the
            centromeric region in interphase
  JOURNAL   Genes Cells 9 (2), 105-120 (2004)
   PUBMED   15009096
REFERENCE   9  (bases 1 to 2494)
  AUTHORS   Pan,H.Y., Zhang,Y.J., Wang,X.P., Deng,J.H., Zhou,F.C. and Gao,S.J.
  TITLE     Identification of a novel cellular transcriptional repressor
            interacting with the latent nuclear antigen of Kaposi's
            sarcoma-associated herpesvirus
  JOURNAL   J Virol 77 (18), 9758-9768 (2003)
   PUBMED   12941884
REFERENCE   10 (bases 1 to 2494)
  AUTHORS   Zhang,Y.J., Pan,H.Y. and Gao,S.J.
  TITLE     Reverse transcription slippage over the mRNA secondary structure of
            the LIP1 gene
  JOURNAL   Biotechniques 31 (6), 1286 (2001)
   PUBMED   11768657
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF469667.1 and AF516710.1.
            
            On Nov 23, 2018 this sequence version replaced NM_024629.3.
            
            Summary: The centromere is a specialized chromatin domain, present
            throughout the cell cycle, that acts as a platform on which the
            transient assembly of the kinetochore occurs during mitosis. All
            active centromeres are characterized by the presence of long arrays
            of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see
            MIM 601128). MLF1IP, or CENPU, is an additional factor required for
            centromere assembly (Foltz et al., 2006 [PubMed
            16622419]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the protein-coding
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC131556.1, SRR14038194.2669536.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281453.10/ ENSP00000281453.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1574              AF469667.1         40-1613
            1575-2494           AF516710.1         2027-2946
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q35.1"
     gene            1..2494
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /note="centromere protein U"
                     /db_xref="GeneID:79682"
                     /db_xref="HGNC:HGNC:21348"
                     /db_xref="MIM:611511"
     exon            1..81
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1291
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /note="KSHV latent nuclear antigen interacting protein 1;
                     polo-box-interacting protein 1; centromere protein of 50
                     kDa; interphase centromere complex protein 24; MLF1
                     interacting protein"
                     /codon_start=1
                     /product="centromere protein U"
                     /protein_id="NP_078905.2"
                     /db_xref="CCDS:CCDS3838.1"
                     /db_xref="GeneID:79682"
                     /db_xref="HGNC:HGNC:21348"
                     /db_xref="MIM:611511"
                     /translation="MAPRGRRRPRPHRSEGARRSKNTLERTHSMKDKAGQKCKPIDVF
                     DFPDNSDVSSIGRLGENEKDEETYETFDPPLHSTAIYADEEEFSKHCGLSLSSTPPGK
                     EAKRSSDTSGNEASEIESVKISAKKPGRKLRPISDDSESIEESDTRRKVKSAEKISTQ
                     RHEVIRTTASSELSEKPAESVTSKKTGPLSAQPSVEKENLAIESQSKTQKKGKISHDK
                     RKKSRSKAIGSDTSDIVHIWCPEGMKTSDIKELNIVLPEFEKTHLEHQQRIESKVCKA
                     AIATFYVNVKEQFIKMLKESQMLTNLKRKNAKMISDIEKKRQRMIEVQDELLRLEPQL
                     KQLQTKYDELKERKSSLRNAAYFLSNLKQLYQDYSDVQAQEPNVKETYDSSSLPALLF
                     KARTLLGAESHLRNINHQLEKLLDQG"
     exon            82..130
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            131..248
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            249..354
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            355..415
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            416..652
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            653..722
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..831
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            832..910
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            911..958
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1020
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1177
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..2494
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1559..1564
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /note="hexamer: AATAAA"
     polyA_site      1577
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /note="major polyA site"
     regulatory      2475..2480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
                     /note="hexamer: ATTAAA"
     polyA_site      2494
                     /gene="CENPU"
                     /gene_synonym="CENP50; CENPU50; KLIP1; MLF1IP; PBIP1"
ORIGIN      
        1 agcttccagt cgctcgagag cggagagcgg caccatggcc ccgcgggggc ggcggcggcc
       61 gcggcctcac aggtctgagg gcgcaagacg ttcaaagaac actttagaaa gaacacattc
      121 catgaaagat aaagctggtc aaaagtgcaa gcctattgac gtgttcgact ttcctgataa
      181 ttctgatgtc tcaagcattg gcaggctggg tgaaaatgag aaagatgaag aaacttatga
      241 gacctttgat cctcctttac atagcacagc tatatatgct gatgaagaag aattctccaa
      301 acattgtgga ctgtctctct cttcaactcc tccaggaaaa gaagcaaaaa gaagttcaga
      361 cacttctgga aatgaagcaa gtgaaatcga atctgtaaaa attagtgcaa aaaagccagg
      421 aagaaagctc aggcccatta gtgatgactc tgaaagcatt gaagaaagtg atacaaggag
      481 aaaagttaaa tcagcagaga aaataagtac acaacgtcat gaggttattc gaaccacagc
      541 gtcttcagaa ctttcagaga aaccagctga gtctgtcact tctaaaaaga caggacccct
      601 tagtgcccag ccctctgttg aaaaagagaa cttggcaata gaaagtcaat cgaaaactca
      661 gaaaaaaggg aagatatctc atgacaaaag gaagaaatca agaagtaaag ccataggctc
      721 agatacttct gacattgtgc acatttggtg tccagaagga atgaaaacca gtgacatcaa
      781 ggagttgaat attgttttgc ctgaatttga gaaaacccac ctagagcatc aacaaagaat
      841 agaatctaaa gtttgtaagg cagccatcgc cacattttat gttaatgtta aagaacaatt
      901 catcaaaatg cttaaagaaa gccagatgtt gacaaatctg aaaaggaaga atgctaagat
      961 gatttcagat atcgaaaaga aaaggcagcg tatgattgaa gtccaggatg aactgcttcg
     1021 gttagagcca cagctgaaac aactacaaac aaaatatgat gaacttaaag agagaaagtc
     1081 ttcccttagg aatgcagcat atttcttatc taatttaaaa cagctttatc aagattattc
     1141 agatgttcaa gctcaagaac caaacgtaaa ggaaacgtat gattcatcca gccttccagc
     1201 tctgttattt aaagcaagaa cacttctggg agccgaaagc catctgcgaa atatcaacca
     1261 tcagttagag aagctccttg accagggatg agaagagcag tctactaaaa tgtgcctata
     1321 ggaagactag tctcatgctg ttaccttctg aaactgtacc tttataaatc aattgttttg
     1381 caaagaagtt atggcctact tagaatctaa aatttgttat tcaaattaaa tggctgtgaa
     1441 caatgttaaa tagcatcagt ttgtccaata gttttaaagg ccataatcat cttttctggt
     1501 taatatcttg agtaatttta aaatgttgac accttaatcg gtcccaggta tgagctataa
     1561 taaacttgta aaattaagtt gatgtgaaca taattttgat taattaataa ggcgatttct
     1621 cctgaattta caccaaagct aatttttaat gaaattggtt tacagaagta aaaaacaaaa
     1681 attggaaagc aaagtaataa aacttagttt atataaacag gttgaatgat atatttatca
     1741 aatctcacag acatcaggca aattatagcc tggtgacaaa agtgttcata gtgaattagt
     1801 tactcttgta atacttctat aattagttca tcaggaattt catccacttc actgttataa
     1861 ctgagaagac tgttctctgc agcttcagct aattcagcat cttcagtagc ttctaaaaaa
     1921 taagcatcat caatgccatt atcccagaca gcatcagcag atgcacctgt tgacagcctg
     1981 ctaggtgatg gtttatgagg attctgggtt tcattgctcc tagtttcatc tgcttcatct
     2041 gttgtaaact cttcttcctt tatttcagtg gtgaagggat agagagtggg ataggaaaat
     2101 atttactcag gatatgtgat ttaaccttat actctatgtt gaagtaaggt attaagtgac
     2161 agatactaaa gtgaatatgc aggaggaatg ctgtctccga tatctcaccg tgggaatgag
     2221 tgcactgatt caaacgttgc tgcactgaag ctcagacaca cttgaaactc caaatttgaa
     2281 attacctaca gttctgtgca catacttttc aatactcccc gacggaagag caagggtgga
     2341 tttaattttt taacaagtgg acagtccagc tgaagacaaa tcagaagata aatttgctat
     2401 cttgacaatg gacttagtac ccatgcttta aattttaaag tatttagcaa atcgtaaaca
     2461 tggattgaaa aaagattaaa aacagttgcc aaaa
//
