LOCUS       NM_001322101            4106 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens centromere protein O (CENPO), transcript variant 3,
            mRNA.
ACCESSION   NM_001322101 XM_006712102
VERSION     NM_001322101.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Si,L.H., Sun,G.C., Liu,Z.W., Gu,S.Y., Yan,C.H., Xu,J.Y. and Jia,Y.
  TITLE     High expression levels of centromere protein O participates in cell
            proliferation of human ovarian cancer
  JOURNAL   J Ovarian Res 17 (1), 126 (2024)
   PUBMED   38890751
  REMARK    GeneRIF: High expression levels of centromere protein O
            participates in cell proliferation of human ovarian cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Wang,Y., Ye,D., Li,Y., Lv,F., Shen,W., Li,H., Tian,L., Fan,Z.,
            Li,Y., Wang,Y., Li,F. and Chen,Y.
  TITLE     Prognostic and immune infiltrative biomarkers of CENPO in
            pan-cancer and its relationship with lung adenocarcinoma cell
            proliferation and metastasis
  JOURNAL   BMC Cancer 23 (1), 735 (2023)
   PUBMED   37558987
  REMARK    GeneRIF: Prognostic and immune infiltrative biomarkers of CENPO in
            pan-cancer and its relationship with lung adenocarcinoma cell
            proliferation and metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4106)
  AUTHORS   Tian,T., Chen,L., Dou,Z., Yang,Z., Gao,X., Yuan,X., Wang,C.,
            Liu,R., Shen,Z., Gui,P., Teng,M., Meng,X., Hill,D.L., Li,L.,
            Zhang,X., Liu,X., Sun,L., Zang,J. and Yao,X.
  TITLE     Structural insights into human CCAN complex assembled onto DNA
  JOURNAL   Cell Discov 8 (1), 90 (2022)
   PUBMED   36085283
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4106)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4106)
  AUTHORS   Nguyen,A.L., Fadel,M.D. and Cheeseman,I.M.
  TITLE     Differential requirements for the CENP-O complex reveal parallel
            PLK1 kinetochore recruitment pathways
  JOURNAL   Mol Biol Cell 32 (8), 712-721 (2021)
   PUBMED   33596090
  REMARK    GeneRIF: Differential requirements for the CENP-O complex reveal
            parallel PLK1 kinetochore recruitment pathways.
REFERENCE   6  (bases 1 to 4106)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   7  (bases 1 to 4106)
  AUTHORS   Izuta,H., Ikeno,M., Suzuki,N., Tomonaga,T., Nozaki,N., Obuse,C.,
            Kisu,Y., Goshima,N., Nomura,F., Nomura,N. and Yoda,K.
  TITLE     Comprehensive analysis of the ICEN (Interphase Centromere Complex)
            components enriched in the CENP-A chromatin of human cells
  JOURNAL   Genes Cells 11 (6), 673-684 (2006)
   PUBMED   16716197
REFERENCE   8  (bases 1 to 4106)
  AUTHORS   Okada,M., Cheeseman,I.M., Hori,T., Okawa,K., McLeod,I.X.,
            Yates,J.R. 3rd, Desai,A. and Fukagawa,T.
  TITLE     The CENP-H-I complex is required for the efficient incorporation of
            newly synthesized CENP-A into centromeres
  JOURNAL   Nat Cell Biol 8 (5), 446-457 (2006)
   PUBMED   16622420
REFERENCE   9  (bases 1 to 4106)
  AUTHORS   Foltz,D.R., Jansen,L.E., Black,B.E., Bailey,A.O., Yates,J.R. 3rd
            and Cleveland,D.W.
  TITLE     The human CENP-A centromeric nucleosome-associated complex
  JOURNAL   Nat Cell Biol 8 (5), 458-469 (2006)
   PUBMED   16622419
REFERENCE   10 (bases 1 to 4106)
  AUTHORS   Obuse,C., Yang,H., Nozaki,N., Goto,S., Okazaki,T. and Yoda,K.
  TITLE     Proteomics analysis of the centromere complex from HeLa interphase
            cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of
            the CEN-complex, while BMI-1 is transiently co-localized with the
            centromeric region in interphase
  JOURNAL   Genes Cells 9 (2), 105-120 (2004)
   PUBMED   15009096
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CF993857.1, BX406097.2,
            AK027859.1, AC012073.9 and BX110829.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001322101.1.
            
            Summary: This gene encodes a component of the interphase centromere
            complex. The encoded protein is localized to the centromere
            throughout the cell cycle and is required for bipolar spindle
            assembly, chromosome segregation and checkpoint signaling during
            mitosis. Alternatively spliced transcript variants encoding
            multiple protein isoforms have been observed for this gene.
            [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (3) and variant 1 both encode
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.102142.1,
                                           SRR5189661.96959.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380834.7/ ENSP00000370214.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                CF993857.1         338-359
            23-836              BX406097.2         8-821
            837-848             AK027859.1         767-778
            849-849             AC012073.9         19447-19447
            850-950             AK027859.1         780-880
            951-951             AC012073.9         20401-20401
            952-1485            AK027859.1         882-1415
            1486-3597           AC012073.9         22448-24559
            3598-4106           BX110829.1         1-509
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..4106
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /note="centromere protein O"
                     /db_xref="GeneID:79172"
                     /db_xref="HGNC:HGNC:28152"
                     /db_xref="MIM:611504"
     exon            1..77
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     exon            78..191
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1048
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /note="isoform 1 is encoded by transcript variant 3;
                     centromeric protein O; interphase centromere complex
                     protein 36"
                     /codon_start=1
                     /product="centromere protein O isoform 1"
                     /protein_id="NP_001309030.1"
                     /db_xref="CCDS:CCDS1714.1"
                     /db_xref="GeneID:79172"
                     /db_xref="HGNC:HGNC:28152"
                     /db_xref="MIM:611504"
                     /translation="MEQANPLRPDGESKGGVLAHLERLETQVSRSRKQSEELQSVQAQ
                     EGALGTKIHKLRRLRDELRAVVRHRRASVKACIANVEPNQTVEINEQEALEEKLENVK
                     AILQAYHFTGLSGKLTSRGVCVCISTAFEGNLLDSYFVDLVIQKPLRIHHHSVPVFIP
                     LEEIAAKYLQTNIQHFLFSLCEYLNAYSGRKYQADRLQSDFAALLTGPLQRNPLCNLL
                     SFTYKLDPGGQSFPFCARLLYKDLTATLPTDVTVTCQGVEVLSTSWEEQRASHETLFC
                     TKPLHQVFASFTRKGEKLDMSLVS"
     exon            192..361
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     exon            362..479
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     exon            480..739
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     exon            740..911
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1083
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..4106
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4083..4088
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /note="hexamer: AATAAA"
     polyA_site      4106
                     /gene="CENPO"
                     /gene_synonym="CENP-O; ICEN-36; MCM21R"
                     /note="major polyA site"
ORIGIN      
        1 aggatcgcaa agcacgccgg gaccggttgg tttggttttg aagacgtgga tggcgggaat
       61 tctcgcttct ggcctggcaa ggacattgga gtccctatca ccggttgcct agacaacttc
      121 atgggaaggc ccttgggaat ctgagatgga gcaggcgaac cctttacgtc cagatggcga
      181 gtccaaagga ggtgttttag ctcacttgga aaggctagag acccaagtga gcagatcccg
      241 taaacagtct gaagagctgc agagcgtgca ggcccaggaa ggtgctcttg gaaccaagat
      301 tcataaacta aggcgtctgc gagatgagct gagggctgtg gtgcggcacc ggcgagccag
      361 cgtgaaagca tgtattgcca atgtagaacc caaccaaaca gtggagatca atgagcaaga
      421 agcattggaa gagaaattgg aaaatgtgaa agccattctg caggcatatc attttacagg
      481 cctcagtggt aaactgacca gccgaggagt ttgtgtctgc atcagtactg cttttgaggg
      541 gaacctattg gattcctatt ttgtggacct tgtcatacag aaaccactcc ggatacatca
      601 ccattcagtc ccagtcttca ttcccctgga agagatagct gcaaaatatt tacagaccaa
      661 catccagcac ttcctgttca gtctctgcga gtacctgaat gcttactctg ggaggaagta
      721 ccaggcagac cggcttcaga gtgactttgc agccctcctg actgggccct tgcagagaaa
      781 cccactgtgt aacttgctgt catttactta caaactggat ccagggggtc agtccttccc
      841 gttctgtgct agattgctgt ataaggacct cacagcaact cttcccactg acgtcaccgt
      901 gacatgtcaa ggagtggaag tattatccac ttcatgggag gagcaacgag catctcatga
      961 aactctgttc tgtacgaagc ccttgcatca agtgtttgcc tcatttacaa gaaaaggaga
     1021 aaagttggat atgagtctgg tctcctaata gattgttttc actgcactgg gagcacatca
     1081 gagaaataaa tcccccctcc cctgccaggt gaaaggaaat attgcacttt ctgttctcat
     1141 gactaagggg acaggagttc cagaagaacc tttcaagatg atcaggaaca ccaggacgag
     1201 ggccgtctca cctcactcgg accacatgga gacctccctt caaaatggga gccatgtcct
     1261 gccccaccaa gccctgtctg aagtggagct tccccgcctg tgctccctcc acagtcccgg
     1321 aaagcccagc ggcaaaggca gctttgtccc agctctgcca ccctcctgct cacagtggtc
     1381 agggcccctc aggggcaagg acggcaggga ttggaacgag ggctctggaa ggactgttca
     1441 gccctatgcc taagacccct atgctgggga cactacaggc acacacagga atagcagggc
     1501 caccctcaga gctcacacat ccacgaacaa atgaaggctg aggaggtttc taaacctaaa
     1561 gtccatgagt gtgcacttca atccaggaag gtcgggactt ccttcagttt caaaaaataa
     1621 attctccctt ccggtttgga ctgttgcagg ctcgaggcca ttcaggagtt gtccaccacc
     1681 tggtggggca gtgtgacaga ggggccattg gggaaggtgg ctagcttatc ccgccccttc
     1741 aagaagaagg tcagcagctc ccccttcccc ttcacaaaga tggggcctcg cctcacaaag
     1801 cggaagccgt actctcggag gatgacttgg gtttcttcta ccacctggag agggaggggg
     1861 agcaagaacg tggcgttacg gggggagcct agactgaggg cgggtggggg ctttgggtgg
     1921 ttggagccga gcactgatcc atgggtccca agcagtacgg gacactcccc aaacctccca
     1981 gggccaagcc cttccacccg tggcgagcag cgggtgggaa ggagaaccct ggagtgactg
     2041 gctgggggcc tcctctcatc cagagacttc tctcctagga tggccatggt cacctgggtg
     2101 gcagcactgt tacctggaaa ctgccactgc ctgctcttct gtccctttgc ccctttcgtg
     2161 gagcttttct gccagacgcc actgagacag atcacaaggt attagaaggt tcatacccaa
     2221 aggtaggcca tatgcatcta gaacttcagc ccagattttg tggatgggtg gaagtgtttc
     2281 ttcctgtgct gaggctagct attgcagaga ttcttttcca cttgccccac gtctctgcct
     2341 ctggacttac tgttcagggc cagggtggga ggcaggggca cgtgggaaag cactgttccg
     2401 gttttgttct catgccgagt ctgagcacgt gccagctgtg ccactggaca tacctgaatg
     2461 ttgcccatga cccccgtgga ctccatcctg ctggctacat tgactgtatt gccccagatg
     2521 tcgtagtgtg gtttccgggc tccgatgacc ccagccagaa ccccgccttt gttcatgcct
     2581 agggtagagg cataaagttc agcacagcca caggccacac cttgttatgg gcctcagaag
     2641 ccatctcctc tccagacctg taccacaaag ctcctaatgt aacacatcat tgtcctcatt
     2701 caacttggct gtatgctatt ggagggtgga aatcacatct cctgtttatc cgtgtgcttg
     2761 ttaggtgtca gccgccaccc cccccccata tgcagattta ctcggcatgg tagtggccag
     2821 cttctaacac agctggtatt tcaagtctcc tgggacctca ctcaggaatg ataccccctc
     2881 agtagaagca gcaggtgatc ttaactcctt tcaaagagca ggcctgtctg ggaagccatg
     2941 tcctcagcag gcacagcaac ccctctggaa atggatcaca aactcacttc tcagccaggc
     3001 aggccaagct tctattgtaa cagtaggcac agtatagtcg gatcatcaca tcagctgggt
     3061 ttttggttta gtcatctaga gtcgtctgga ctaaaggtct ttcaggtctc cttgccctgt
     3121 gagtgcgtga acctccccac ccgaattgcc tcagttgtcc tgagcctcat gtctctcctg
     3181 gtggtgggcc aggcccctgc atgggaaggg agcctgctgc ggggcaggcc agctgggggt
     3241 gctcacctat gcgcagcatg aagttattga aggactggtt gttgatgttg gtgagcgtat
     3301 ccttcatggc cagcgcgaag tcggccaggt cagccaggtg ctgccagcgc tctctctcgg
     3361 acttgtcttc ctgtgccagg ggaccgtgga gaaagtgtca ggggccgctc actgcagcag
     3421 cctgctctgc tgccttccct ggcagtgttc tgggggtgga ttccctacac ctagatgttc
     3481 aaggccttac ttttcctccc acaaaggagt cgcagccacg ctagctctga cttgccactg
     3541 tgacaaagtt cacgtagcag gtctaggcaa agactgggca attgagcaga ggagacggac
     3601 ctgtgagtct gaccacgagg cggacccctt caccttggct gggcctggtc ctggtcctta
     3661 ggttttgtca ggttgtcctt gtttggatcc ctcaactagg tgataagcac tggaggggga
     3721 tgacccgcct tggacgtgtt tctttaacct catccatata atagggccgt gggatggttg
     3781 tagaggtaaa gcaggatgat ggtgttttaa gaccagagct tgggaccagg gctcctacac
     3841 ctaattttct ctcctggtag ctgaacaaag gtctaaatta gcttaacaaa agaacaggct
     3901 gccgtcagcc agagttctga aggccatgct ttcagtttcc cttgttgaca attgctctcc
     3961 agttcctatg aaagcacaga gccttagggg gcctggccac agaacacaac catcttaggc
     4021 ctgagctgtg aacagcaggg ggttgtgtgt ctgttctgtt tctctgcttg ccgaactttc
     4081 tcaataaacc ctatttctta tttata
//
