LOCUS       NM_001100624            3928 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens centromere protein N (CENPN), transcript variant 2,
            mRNA.
ACCESSION   NM_001100624
VERSION     NM_001100624.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Liu,R., Dou,Z., Tian,T., Gao,X., Chen,L., Yuan,X., Wang,C., Hao,J.,
            Gui,P., Mullen,M., Aikhionbare,F., Niu,L., Bi,G., Zou,P., Zhang,X.,
            Fu,C., Yao,X., Zang,J. and Liu,X.
  TITLE     Dynamic phosphorylation of CENP-N by CDK1 guides accurate
            chromosome segregation in mitosis
  JOURNAL   J Mol Cell Biol 15 (6) (2023)
   PUBMED   37365681
  REMARK    GeneRIF: Dynamic phosphorylation of CENP-N by CDK1 guides accurate
            chromosome segregation in mitosis.
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Gui,Z., Tian,Y., Yu,T., Liu,S., Liu,C. and Zhang,L.
  TITLE     Clinical implications and immune features of CENPN in breast cancer
  JOURNAL   BMC Cancer 23 (1), 851 (2023)
   PUBMED   37697245
  REMARK    GeneRIF: Clinical implications and immune features of CENPN in
            breast cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3928)
  AUTHORS   Xu,S. and Wei,X.
  TITLE     Knockdown of CENPN Inhibits Glucose Metabolism and Induces G1
            Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT Signaling
            Pathway
  JOURNAL   Horm Metab Res 55 (8), 563-572 (2023)
   PUBMED   37127048
  REMARK    GeneRIF: Knockdown of CENPN Inhibits Glucose Metabolism and Induces
            G1 Arrest in Esophageal Cancer Cells by Regulating PI3K/AKT
            Signaling Pathway.
REFERENCE   4  (bases 1 to 3928)
  AUTHORS   Tian,T., Chen,L., Dou,Z., Yang,Z., Gao,X., Yuan,X., Wang,C.,
            Liu,R., Shen,Z., Gui,P., Teng,M., Meng,X., Hill,D.L., Li,L.,
            Zhang,X., Liu,X., Sun,L., Zang,J. and Yao,X.
  TITLE     Structural insights into human CCAN complex assembled onto DNA
  JOURNAL   Cell Discov 8 (1), 90 (2022)
   PUBMED   36085283
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3928)
  AUTHORS   Zhou,K., Gebala,M., Woods,D., Sundararajan,K., Edwards,G.,
            Krzizike,D., Wereszczynski,J., Straight,A.F. and Luger,K.
  TITLE     CENP-N promotes the compaction of centromeric chromatin
  JOURNAL   Nat Struct Mol Biol 29 (4), 403-413 (2022)
   PUBMED   35422519
  REMARK    GeneRIF: CENP-N promotes the compaction of centromeric chromatin.
REFERENCE   6  (bases 1 to 3928)
  AUTHORS   Carroll,C.W., Silva,M.C., Godek,K.M., Jansen,L.E. and Straight,A.F.
  TITLE     Centromere assembly requires the direct recognition of CENP-A
            nucleosomes by CENP-N
  JOURNAL   Nat Cell Biol 11 (7), 896-902 (2009)
   PUBMED   19543270
  REMARK    GeneRIF: Data suggest that CENP-N interprets the information
            encoded within CENP-A nucleosomes and recruits other proteins to
            centromeric chromatin that are required for centromere function and
            propagation.
REFERENCE   7  (bases 1 to 3928)
  AUTHORS   Izuta,H., Ikeno,M., Suzuki,N., Tomonaga,T., Nozaki,N., Obuse,C.,
            Kisu,Y., Goshima,N., Nomura,F., Nomura,N. and Yoda,K.
  TITLE     Comprehensive analysis of the ICEN (Interphase Centromere Complex)
            components enriched in the CENP-A chromatin of human cells
  JOURNAL   Genes Cells 11 (6), 673-684 (2006)
   PUBMED   16716197
REFERENCE   8  (bases 1 to 3928)
  AUTHORS   Okada,M., Cheeseman,I.M., Hori,T., Okawa,K., McLeod,I.X.,
            Yates,J.R. 3rd, Desai,A. and Fukagawa,T.
  TITLE     The CENP-H-I complex is required for the efficient incorporation of
            newly synthesized CENP-A into centromeres
  JOURNAL   Nat Cell Biol 8 (5), 446-457 (2006)
   PUBMED   16622420
REFERENCE   9  (bases 1 to 3928)
  AUTHORS   Foltz,D.R., Jansen,L.E., Black,B.E., Bailey,A.O., Yates,J.R. 3rd
            and Cleveland,D.W.
  TITLE     The human CENP-A centromeric nucleosome-associated complex
  JOURNAL   Nat Cell Biol 8 (5), 458-469 (2006)
   PUBMED   16622419
REFERENCE   10 (bases 1 to 3928)
  AUTHORS   Obuse,C., Yang,H., Nozaki,N., Goto,S., Okazaki,T. and Yoda,K.
  TITLE     Proteomics analysis of the centromere complex from HeLa interphase
            cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of
            the CEN-complex, while BMI-1 is transiently co-localized with the
            centromeric region in interphase
  JOURNAL   Genes Cells 9 (2), 105-120 (2004)
   PUBMED   15009096
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK023669.1, HY167728.1,
            AC092718.3 and BC039021.2.
            
            On Aug 11, 2020 this sequence version replaced NM_001100624.2.
            
            Summary: The protein encoded by this gene forms part of the
            nucleosome-associated complex and is important for kinetochore
            assembly. It is bound to kinetochores during S phase and G2 and
            recruits other proteins to the centromere. Pseudogenes of this gene
            are located on chromosome 2. Alternative splicing results in
            multiple transcript variants that encode different protein
            isoforms. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (2) uses an alternate 3' terminal
            exon compared to variant 1. The resulting protein (isoform 2) has a
            shorter and distinct C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.3388445.1, AK023669.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305850.10/ ENSP00000305608.5
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               AK023669.1         717-1041
            326-326             HY167728.1         293-293
            327-1765            AK023669.1         1043-2481
            1766-2320           AC092718.3         152956-153510       c
            2321-2777           BC039021.2         15-471
            2778-3229           AC092718.3         152047-152498       c
            3230-3928           BC039021.2         472-1170
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.2"
     gene            1..3928
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="centromere protein N"
                     /db_xref="GeneID:55839"
                     /db_xref="HGNC:HGNC:30873"
                     /db_xref="MIM:611509"
     exon            1..64
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            65..245
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1094
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="isoform 2 is encoded by transcript variant 2;
                     interphase centromere complex protein 32"
                     /codon_start=1
                     /product="centromere protein N isoform 2"
                     /protein_id="NP_001094094.2"
                     /db_xref="CCDS:CCDS42200.1"
                     /db_xref="GeneID:55839"
                     /db_xref="HGNC:HGNC:30873"
                     /db_xref="MIM:611509"
                     /translation="MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQR
                     KESVVQHLIHLCEEKRASISDAALLDIIYMQFHQHQKVWEVFQMSKGPGEDVDLFDMK
                     QFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPY
                     AFTSSSMLRRNTPLLGQALTIASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNS
                     TTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFK
                     SGLNGSILAEREEPLRCLIKFSSPHLLEALKSLAPAGIADAPLSPLLTCIPNKRMNYF
                     KIRDK"
     exon            246..291
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            292..351
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            352..428
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            429..605
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            606..707
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            708..771
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            772..884
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1011
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..3928
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1734..1739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="hexamer: AATAAA"
     polyA_site      1773
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="major polyA site"
     regulatory      3737..3742
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="hexamer: AATAAA"
     polyA_site      3761
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="major polyA site"
     regulatory      3905..3910
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="hexamer: AATAAA"
     polyA_site      3928
                     /gene="CENPN"
                     /gene_synonym="BM039; C16orf60; CENP-N; ICEN32"
                     /note="major polyA site"
ORIGIN      
        1 ggctttgaag gcgcggcgag cgggaacagc tcttgaggag tgagactgca ggagatgtgg
       61 gccgtgccaa agagatggat gagactgttg ctgagttcat caagaggacc atcttgaaaa
      121 tccccatgaa tgaactgaca acaatcctga aggcctggga ttttttgtct gaaaatcaac
      181 tgcagactgt aaatttccga cagagaaagg aatctgtagt tcagcacttg atccatctgt
      241 gtgaggaaaa gcgtgcaagt atcagtgatg ctgccctgtt agacatcatt tatatgcaat
      301 ttcatcagca ccagaaagtt tgggaagttt ttcagatgag taaaggacca ggtgaagatg
      361 ttgacctttt tgatatgaaa caatttaaaa attcgttcaa gaaaattctt cagagagcat
      421 taaaaaatgt gacagtcagc ttcagagaaa ctgaggagaa tgcagtctgg attcgaattg
      481 cctggggaac acagtacaca aagccaaacc agtacaaacc tacctacgtg gtgtactact
      541 cccagactcc gtacgccttc acgtcctcct ccatgctgag gcgcaataca ccgcttctgg
      601 gtcaggcgct gacaattgct agcaaacacc atcagattgt gaaaatggac ctgagaagtc
      661 ggtatctgga ctctcttaag gctattgttt ttaaacagta taatcagacc tttgaaactc
      721 acaactctac gacacctcta caggaaagaa gccttggact agatataaat atggattcaa
      781 ggatcattca tgaaaacata gtagaaaaag agagagtcca acgaataact caagaaacat
      841 ttggagatta tcctcaacca caactagaat ttgcacaata taagcttgaa acgaaattca
      901 aaagtggttt aaatgggagc atcttggctg agagggaaga acccctccga tgcctaataa
      961 agttctctag cccacatctt ctggaagcat tgaaatcctt agcaccagcg ggtattgcag
     1021 atgctccact ttctccactg ctcacttgca tacccaacaa gagaatgaat tattttaaaa
     1081 ttagagataa ataagacgtg cgtggtttct taagcacagc tcctccttct tgatattgca
     1141 catgcacttc agttcatggc tagctgtata gcttccgtct gtaaacttgt attttcaaga
     1201 atccttggta ttgaattttt agaaatgctc acataattgt tgggactgat tcattcctcc
     1261 acgatatgcc tcctctctct gatatcctgc taactgtagc cgttgtggca tttgagatga
     1321 caggacatat atatatatgg ccccacactt gaccttgagt gcctgaatgc tctgaaatca
     1381 agcatatggc acagcgctca agacttttgg gtttgtgtcc ttttttctat ggctgtctct
     1441 tctcaattct ggagaggtct ggttccagtg gctggtttcc agggattgat tcttaagctc
     1501 tggatcacag agagaagcaa caaggaacta tactcaactc aaaacttttt aggagaatca
     1561 tgaaattggt ctattcaaag gatggagttg agtccattct gttattgttg caagaggttg
     1621 catatttggt gagtcagtta tataaaatag tgttcttatt gtaaatatga tacttctcat
     1681 aatctatttt atcatgtgta taacattcaa actgacaaat atattgactt atgaataaag
     1741 gtgtcaaaaa actggcacat cagttaattt tgatcaaagt acttcagtga tcatcactaa
     1801 ataccctatc tttttaaaaa ttttttcctt tctaattttt tatttcttta tttatttatt
     1861 gagacagggt ctcactgtgt cacccaagct ggagtgcagt ggcatgatca tggctcactg
     1921 cagcctggac ctcccaggct caagtgatcc ttccacctca gcctcccgaa tagctgggat
     1981 tacagacgcg ctccatcctg cccagctaat tttttttttg tttgtttttt gttttttgag
     2041 acaagtctcc ttctatcgcc caggctggag tgcaatggca caatcttggc tccctgcaac
     2101 ctctgcctcc tgggttgaag agattctcct gcctcagcct ctgagtagct gggactacag
     2161 gcctgcacca ccatgcccag ctaatttttg tatttttagt agagacaggg tttctccatg
     2221 ttgcccaggc tgatctcaaa ctcctgggct caagcaatct gcccatttta gcctcctaaa
     2281 atgctgggat tataggagtg tattagtcta ttctcatgct gctaataaag atacctaaga
     2341 ctgggtaatt cacaaaggaa agaggcttaa ctgacgcaca tttccacgtg gccagggagg
     2401 cctcacaatc atggtggaag gcaaatgaga agcaaagtca tgtcttacat ggcggcaggc
     2461 aagagagctt gtgcagggaa actcccattt ataaaaccac cagagctcat gaaacttatt
     2521 cactaccatg agaatagtat gggggaaatc gttcccatga ctcaagtatc tccacctggc
     2581 cctgcccttg tcacatgggg gttattacaa ttcaaggtga cacttgtgtg gagacacagc
     2641 caaaccatat cacaggcatg agctaccacg cccgccagca tgtctttttt aaacatttta
     2701 aaatctattc tttatcttgt ttcagagagg atttggagtt acagaaacac attagactgg
     2761 gcatggtggc tcacacttgt aatcccagca cttcaggagg tttctcctag tgtactctca
     2821 cacttctgat accagatatg tgggggaggg ggtttccccc acacattaag caagtgtgaa
     2881 acatgatata gaaaaatgac ttcactctgg gccgggtgta gtggctcacg cctgtaatcc
     2941 tagcactttg ggaagccgag gtgggcagat cacccgaggt caggagttcg agaccagcct
     3001 agccaacatg gcaaaacctt gtctctacta aaaatagaaa aattagttgg gggtagtggc
     3061 agccgcctgt aatcccagct attcgagagg ctgaggcagg agaactggtt gaaccgggga
     3121 ggcagaggtt gcagtgagcc aagatcatgc cactgcactc tagcctgggt gacagagtga
     3181 aactctgcct caaaaaataa aataaaggcc aggcatggtg gctcacgcct ataatcccag
     3241 cactttcaga ggccaaggca ggtgggtcac aaggtcaaga gtttgagact agccttagca
     3301 acatggtgaa ggtgaacccc gactctacta aaaatacaaa aattagctgg gcctggtggt
     3361 gtgcacctgt aatcccagct acttaggagg ctgaggcagg agaatcgctt gaacccggga
     3421 gacataggtt gcagtgagcc gagactgcac cactgcactc cagcctaggt gacagagtga
     3481 gactccatct taaaaaataa ataaataaaa taaaataaat gacatcactt tggttcagag
     3541 ctctaaaatg gagggaggaa gccattctaa aaaggactcc ctacatgacc tgcaacttga
     3601 aaaaaaatta aaagctccaa aaaaaaaaca atacaggagc ttaccttgaa cctttgaatt
     3661 gggccaaatt gcgatgacca ctgcatcctg gaaaatttta tttcaccagc actacaactc
     3721 ctcaacagca ccaaccaata aactatggat ttttgtacta agccagttgc ctctttcaaa
     3781 acaacttgtc aacttgtcta atcaccctca gcttttttta aaaacccctc ctctaccctc
     3841 tctcttcaga acacaagtgg cttctagctg aatctgtctc ccaaattgca attcctaaga
     3901 cctcaataaa aacaccttgt cttgctgc
//
