LOCUS       NM_018451               5082 bp    mRNA    linear   PRI 16-FEB-2025
DEFINITION  Homo sapiens centrosome assembly and centriole elongation protein
            (CPAP), transcript variant 1, mRNA.
ACCESSION   NM_018451
VERSION     NM_018451.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Jaiswal,S., Sanghi,S. and Singh,P.
  TITLE     Separation-of-function MCPH-associated mutations in CPAP affect
            centriole number and length
  JOURNAL   J Cell Sci 136 (21) (2023)
   PUBMED   37823337
  REMARK    GeneRIF: Separation-of-function MCPH-associated mutations in CPAP
            affect centriole number and length.
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Vijayan,K., Arang,N., Wei,L., Morrison,R., Geiger,R., Parks,K.R.,
            Lewis,A.J., Mast,F.D., Douglass,A.N., Kain,H.S., Aitchison,J.D.,
            Johnson,J.S., Aderem,A. and Kaushansky,A.
  TITLE     A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host
            regulator of altered microtubule organization during Plasmodium
            liver infection
  JOURNAL   Cell Chem Biol 29 (9), 1419-1433 (2022)
   PUBMED   35738280
  REMARK    GeneRIF: A genome-wide CRISPR-Cas9 screen identifies CENPJ as a
            host regulator of altered microtubule organization during
            Plasmodium liver infection.
REFERENCE   3  (bases 1 to 5082)
  AUTHORS   Vasquez-Limeta,A., Lukasik,K., Kong,D., Sullenberger,C.,
            Luvsanjav,D., Sahabandu,N., Chari,R. and Loncarek,J.
  TITLE     CPAP insufficiency leads to incomplete centrioles that duplicate
            but fragment
  JOURNAL   J Cell Biol 221 (5) (2022)
   PUBMED   35404385
  REMARK    GeneRIF: CPAP insufficiency leads to incomplete centrioles that
            duplicate but fragment.
REFERENCE   4  (bases 1 to 5082)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5082)
  AUTHORS   Khan,N.M., Masoud,M.S., Baig,S.M., Qasim,M. and Chang,J.
  TITLE     Identification of Pathogenic Mutations in Primary Microcephaly-
            (MCPH-) Related Three Genes CENPJ, CASK, and MCPH1 in
            Consanguineous Pakistani Families
  JOURNAL   Biomed Res Int 2022, 3769948 (2022)
   PUBMED   35281599
  REMARK    GeneRIF: Identification of Pathogenic Mutations in Primary
            Microcephaly- (MCPH-) Related Three Genes CENPJ, CASK, and MCPH1 in
            Consanguineous Pakistani Families.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5082)
  AUTHORS   Iouzalen,N., Andreae,S., Hannier,S. and Triebel,F.
  TITLE     LAP, a lymphocyte activation gene-3 (LAG-3)-associated protein that
            binds to a repeated EP motif in the intracellular region of LAG-3,
            may participate in the down-regulation of the CD3/TCR activation
            pathway
  JOURNAL   Eur J Immunol 31 (10), 2885-2891 (2001)
   PUBMED   11592063
REFERENCE   7  (bases 1 to 5082)
  AUTHORS   Mayor,T., Stierhof,Y.D., Tanaka,K., Fry,A.M. and Nigg,E.A.
  TITLE     The centrosomal protein C-Nap1 is required for cell cycle-regulated
            centrosome cohesion
  JOURNAL   J Cell Biol 151 (4), 837-846 (2000)
   PUBMED   11076968
REFERENCE   8  (bases 1 to 5082)
  AUTHORS   Hung,L.Y., Tang,C.J. and Tang,T.K.
  TITLE     Protein 4.1 R-135 interacts with a novel centrosomal protein (CPAP)
            which is associated with the gamma-tubulin complex
  JOURNAL   Mol Cell Biol 20 (20), 7813-7825 (2000)
   PUBMED   11003675
REFERENCE   9  (bases 1 to 5082)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
REFERENCE   10 (bases 1 to 5082)
  AUTHORS   Verloes,A., Drunat,S., Gressens,P. and Passemard,S.
  TITLE     Primary Autosomal Recessive Microcephalies and Seckel Syndrome
            Spectrum Disorders - RETIRED CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301772
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL354798.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018451.4.
            
            Summary: This gene encodes a protein that belongs to the centromere
            protein family. During cell division, this protein plays a
            structural role in the maintenance of centrosome integrity and
            normal spindle morphology, and it is involved in microtubule
            disassembly at the centrosome. This protein can function as a
            transcriptional coactivator in the Stat5 signaling pathway, and
            also as a coactivator of NF-kappaB-mediated transcription, likely
            via its interaction with the coactivator p300/CREB-binding protein.
            Mutations in this gene are associated with primary autosomal
            recessive microcephaly, a disorder characterized by severely
            reduced brain size and cognitive disability. Alternatively spliced
            transcript variants have been found for this gene. [provided by
            RefSeq, Apr 2012].
            
            Transcript Variant: This variant (1) represents the protein coding
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC113664.1, AK292406.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381884.9/ ENSP00000371308.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AL354798.13        84521-84621         c
            102-611             AL354798.13        74342-74851         c
            612-735             AL354798.13        73706-73829         c
            736-1032            AL354798.13        71550-71846         c
            1033-1141           AL354798.13        69778-69886         c
            1142-1244           AL354798.13        68851-68953         c
            1245-2859           AL354798.13        67106-68720         c
            2860-2992           AL354798.13        65686-65818         c
            2993-3158           AL354798.13        61179-61344         c
            3159-3383           AL354798.13        54403-54627         c
            3384-3468           AL354798.13        51076-51160         c
            3469-3533           AL354798.13        47366-47430         c
            3534-3644           AL354798.13        47036-47146         c
            3645-3785           AL354798.13        46083-46223         c
            3786-3870           AL354798.13        45921-46005         c
            3871-3991           AL354798.13        45723-45843         c
            3992-5082           AL354798.13        44039-45129         c
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.12-q12.13"
     gene            1..5082
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /note="centrosome assembly and centriole elongation
                     protein"
                     /db_xref="GeneID:55835"
                     /db_xref="HGNC:HGNC:17272"
                     /db_xref="MIM:609279"
     exon            1..101
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            102..611
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..4184
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /note="LYST-interacting protein LIP1; LAG-3-associated
                     protein; LYST-interacting protein LIP7; LYST-interacting
                     protein 1; Spindle assembly abnormal 4; centromere protein
                     J"
                     /codon_start=1
                     /product="centrosomal P4.1-associated protein"
                     /protein_id="NP_060921.3"
                     /db_xref="CCDS:CCDS9310.1"
                     /db_xref="GeneID:55835"
                     /db_xref="HGNC:HGNC:17272"
                     /db_xref="MIM:609279"
                     /translation="MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEK
                     RAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCI
                     KKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQL
                     KRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCF
                     PSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEK
                     NNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGER
                     KQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKF
                     QKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGS
                     VTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPK
                     PCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWER
                     EKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQK
                     TNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKST
                     SENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGP
                     FKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERT
                     WTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRR
                     SRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELE
                     TEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQK
                     ERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQM
                     LVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQ
                     VEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEED
                     QDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQ
                     RVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEE
                     SIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRS
                     GRIRVKDKEGNVLMDTEL"
     exon            612..735
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            736..1032
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1141
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1244
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..2859
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..2992
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            2993..3158
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3159..3383
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3384..3468
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3533
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3644
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3645..3785
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3870
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3871..3991
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     exon            3992..5082
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4301..4306
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /note="hexamer: AATAAA"
     polyA_site      4322
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /note="major polyA site"
     regulatory      5064..5069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
                     /note="hexamer: AATAAA"
     polyA_site      5082
                     /gene="CPAP"
                     /gene_synonym="BM032; CENP-J; CENPJ; LAP; LIP1; MCPH6;
                     Sas-4; SASS4; SCKL4"
ORIGIN      
        1 ggggctccga cagaggcggc gattgtggcg gcccatttgt aaatgctgcg gagattgagg
       61 tgtcggttcg tgctgctgag ctgcccaggc ttcacggagc ggtgttggaa atcaatagct
      121 cttctagcct ttgcattgtt taaatataat agtgtcattg gactaagatg ttcctgatgc
      181 caacctcttc agagttaaac agtgggcaga acttcctaac ccagtggatg accaatcctt
      241 ctcgggctgg ggtcatatta aatcgtggat ttcctatttt ggaagcagac aaagagaagc
      301 gagcagctgt ggacatttct accagctttc ctattaaagg cacacatttt tctgatagct
      361 tcagctttat aaatgaagaa gattcacttc ttgaagaaca gaagttggag tcaaacaacc
      421 cttacaaacc acagtcagat aaatctgaaa cccatacagc ctttccttgc attaaaaagg
      481 gaccacaggt agcggcatgt cacagtgctc ctggacacca ggaagaaaac aaaaatgact
      541 tcatcccaga tcttgcgagt gaattcaaag aaggggctta taaagaccca ctttttaaaa
      601 aacttgaaca gctgaaagaa gtacaacaga agaagcagga acaattgaag aggcaacagt
      661 tggagcaact acagagactc atggaagaac aagagaagct gctcaccatg gtgtctgggc
      721 agtgcacact tccaggtttg agtttactgc ctgatgatca gagccagaag cacaggtctc
      781 caggaaatac caccactgga gagagagcca catgctgctt cccatcatat gtctacccgg
      841 acccaaccca ggaagaaaca tacccgtcca acattttatc ccatgagcaa agcaacttct
      901 gtagaactgc tcatggagat tttgtcttaa cttcaaaacg tgcgtctcct aatttatttt
      961 ctgaggcaca gtatcaagaa gcacctgtgg aaaaaaataa tttaaaagaa gaaaaccgta
     1021 accatcctac aggagaaagt atcttatgtt gggagaaagt gacagaacag attcaggaag
     1081 caaatgataa gaacttacaa aaacatgatg attcctcaga agtggctaat attgaagaaa
     1141 ggcccattaa agctgctatt ggagaaagga aacagacctt tgaagattac ttagaagaac
     1201 aaattcagtt ggaagagcaa gaactgaagc aaaaacagct gaaggaagca gaaggaccat
     1261 tgccaatcaa agcaaaacca aaacaaccat ttttaaaacg aggagaaggt ttagctagat
     1321 ttactaatgc caaatctaag tttcaaaaag gcaaagaaag taaactagtg actaaccaga
     1381 gcacttccga ggaccagccg ctgtttaaaa tggatagaca gcaactccag cggaaaaccg
     1441 ctcttaaaaa taaagagctg tgtgcagaca accctatcct taaaaaggac agtaaagcta
     1501 gaaccaagag tggttctgtc accctcagtc agaagccgaa aatgctgaag tgtagtaaca
     1561 ggaaaagtct ttctccgtca ggattgaaaa tacagacggg gaagaaatgt gatgggcagt
     1621 ttagagacca gatcaaattt gaaaacaaag tcacatctaa taataaagaa aatgtaactg
     1681 agtgtccaaa accttgcgat actggctgca cagggtggaa taagacacaa ggtaaagaca
     1741 gacttcctct ttcaacaggg ccggccagcc ggctggctgc taagagcccc ataagggaga
     1801 ccatgaaaga gtctgaatct tctcttgacg tttctcttca gaaaaagtta gagacttggg
     1861 aacgagaaaa ggaaaaggaa aatttggaat tagatgaatt tttgttttta gaacaagctg
     1921 ctgatgaaat atcattttct agtaattcct catttgtact gaaaatctta gaaagggatc
     1981 aacagatctg caaaggtcac cggatgtctt ccacccctgt caaagctgtg ccacagaaga
     2041 caaatccggc agatcccatt agtcattgta accgcagtga ggatttggac cacactgcac
     2101 gtgagaagga gagtgagtgt gaagtcgcac ccaaacaact tcattcattg tcctcagctg
     2161 atgaattgag ggaacagcct tgtaaaatca ggaaagccgt ccaaaagagc acttctgaaa
     2221 atcagactga atggaatgca cgtgacgatg aaggtgttcc aaatagtgac agtagcactg
     2281 actctgagga acagcttgat gttaccataa aaccatcgac tgaggataga gagaggggca
     2341 tcagcagcag agaggatagc ccacaagtct gtgatgataa ggggcctttt aaggacacca
     2401 ggacccaaga agataaaagg agagatgttg atctggattt gtctgataaa gattacagta
     2461 gcgatgagtc tatcatggaa agcataaaac ataaagtgtc tgagccctcg agatcctcat
     2521 ccctaagtct gagtaaaatg gactttgatg atgaaagaac ttggactgac cttgaagaga
     2581 atttgtgtaa ccatgatgtt gttcttggga atgaatccac ttatgggacg ccgcagacat
     2641 gctaccctaa taatgaaata ggtatcctgg acaaaacaat aaaaaggaag attgcaccag
     2701 tcaagagggg agaagacttg agcaagtcca ggaggagcag aagtcctcct acatcggagc
     2761 tgatgatgaa attctttcct tctttgaaac caaaaccaaa gtcagattca cacttgggaa
     2821 atgaactcaa gttaaacata agtcaagacc aaccacctgg tgacaatgct cgatcccagg
     2881 ttttgagaga gaaaattatt gaattggaaa cagaaataga aaagtttaaa gctgagaacg
     2941 catctttagc taaacttcgc attgaacgag aaagtgcctt ggaaaaactc aggaaagaaa
     3001 ttgcagactt cgaacaacag aaagcaaaag aattagctcg aatagaagag tttaaaaagg
     3061 aggagatgag gaagctacaa aaggaacgta aagtttttga aaagtatact acagctgcaa
     3121 gaacttttcc agataaaaag gaacgtgaag aaatacagac tttaaaacag caaatagcag
     3181 atttacggga agatttgaaa agaaaggaaa ccaaatggtc aagtacacac agccgtctca
     3241 gaagccagat acaaatgtta gtcagagaga acacagacct ccgggaagaa ataaaagtga
     3301 tggaaagatt ccgactggat gcctggaaga gagcagaagc catagagagc agcctcgagg
     3361 tggagaagaa ggacaagctt gcgaacacat ctgttcgatt tcaaaacagt cagatttctt
     3421 caggaaccca ggtagaaaaa tacaagaaaa attatcttcc aatgcaaggc aatccacctc
     3481 gaagatccaa gtctgcacct cctcgtgatt taggcaattt ggataaggga caagctgcct
     3541 ctcccaggga gccacttgaa ccactgaact tcccagatcc tgaatataaa gaggaggagg
     3601 aagaccaaga catacaggga gaaatcagtc atcctgatgg aaaggtggaa aaggtttata
     3661 agaatgggtg ccgtgttata ctgtttccca atggaactcg aaaggaagtg agtgcagatg
     3721 ggaagaccat cactgtcact ttctttaatg gtgacgtgaa gcaggtcatg ccagaccaaa
     3781 gagtgatcta ctactatgca gctgcccaga ccactcacac gacatacccg gagggactgg
     3841 aagtcttaca tttctcaagt ggacaaatag aaaaacatta cccagatgga agaaaagaaa
     3901 tcacgtttcc tgaccagact gttaaaaact tatttcctga tggacaagaa gaaagcattt
     3961 tcccagatgg tacaattgtc agagtacaac gtgatggcaa caaactcata gagtttaata
     4021 atggccaaag agaactacat actgcccagt tcaagagacg ggaataccca gatggcactg
     4081 ttaaaaccgt atatgcaaac ggtcatcaag aaacgaagta cagatccggt cggataagag
     4141 ttaaggacaa ggagggtaat gtgctaatgg acacggagct gtgacgatcc tcatgtgatc
     4201 atgaagtaac agtaactgac tttttatgtt aaaaaatgta catttactgt ggattctgtt
     4261 taatttattg tgtatgtgtg gggaaaagat tagattctaa aataaaagtt taccctgtgg
     4321 catcttcatt tttatattct ttgaaatgca atgatagctc tcatttataa ttcactaccc
     4381 tgtctttcaa tagactaagg aaagcatttt gactgtttag aaattgtgta caggttttta
     4441 cagtaagtgg gtgtgtttat tggttagaaa tggctataaa aaaaaaccaa gaagtacagg
     4501 aagtacatca cctagatgtc ccacagacac atcagccgta gagcactgga cttgtacaga
     4561 aggtgtactc aaggagtaaa cacgtgtctc tgtagccaaa atattactat gttcagttag
     4621 taccaaaatt agaccatttg atcttctaca gaggatcatt aattattata acagaatggg
     4681 aagttacaac agtggatgaa tataaatagg tcacacacaa caggtagtaa tcatggtgaa
     4741 gagaaagcag ttataacaag gctgactttg taatgtaatg tctcaaaatg ggatacttga
     4801 gagtggaggg gggcatgaac gatgactagc cagtgttaac ccatggacca cagctaccct
     4861 gatgttacca cagtgagatt actgtagcct catggaagca agatgtccct gaaggatggg
     4921 caagtcactt acttacttac ttgtaaattt atataataaa taattacaga ttggtctaga
     4981 tagaccttac ctgtcacata tactatgaat caggttttgt taatgaaatc tatcaattcc
     5041 aggtgactta actgaatatg aaaaataaaa ctttttgaag aa
//
