LOCUS       NM_022909               1354 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens centromere protein H (CENPH), mRNA.
ACCESSION   NM_022909
VERSION     NM_022909.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Tian,T., Chen,L., Dou,Z., Yang,Z., Gao,X., Yuan,X., Wang,C.,
            Liu,R., Shen,Z., Gui,P., Teng,M., Meng,X., Hill,D.L., Li,L.,
            Zhang,X., Liu,X., Sun,L., Zang,J. and Yao,X.
  TITLE     Structural insights into human CCAN complex assembled onto DNA
  JOURNAL   Cell Discov 8 (1), 90 (2022)
   PUBMED   36085283
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1354)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1354)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1354)
  AUTHORS   Lu,G., Hou,H., Lu,X., Ke,X., Wang,X., Zhang,D., Zhao,Y., Zhang,J.,
            Ren,M. and He,S.
  TITLE     CENP-H regulates the cell growth of human hepatocellular carcinoma
            cells through the mitochondrial apoptotic pathway
  JOURNAL   Oncol Rep 37 (6), 3484-3492 (2017)
   PUBMED   28498417
  REMARK    GeneRIF: In summary, CENP-H may be involved in cell proliferation
            and apoptosis of hepatocellular carcinoma (HCC)cells through the
            mitochondrial apoptotic pathway. Combined with previous studies,
            the data provide a new perspective on HCC development and
            progression.
REFERENCE   6  (bases 1 to 1354)
  AUTHORS   Mikami,Y., Hori,T., Kimura,H. and Fukagawa,T.
  TITLE     The functional region of CENP-H interacts with the Nuf2 complex
            that localizes to centromere during mitosis
  JOURNAL   Mol Cell Biol 25 (5), 1958-1970 (2005)
   PUBMED   15713649
REFERENCE   7  (bases 1 to 1354)
  AUTHORS   Obuse,C., Iwasaki,O., Kiyomitsu,T., Goshima,G., Toyoda,Y. and
            Yanagida,M.
  TITLE     A conserved Mis12 centromere complex is linked to heterochromatic
            HP1 and outer kinetochore protein Zwint-1
  JOURNAL   Nat Cell Biol 6 (11), 1135-1141 (2004)
   PUBMED   15502821
REFERENCE   8  (bases 1 to 1354)
  AUTHORS   Obuse,C., Yang,H., Nozaki,N., Goto,S., Okazaki,T. and Yoda,K.
  TITLE     Proteomics analysis of the centromere complex from HeLa interphase
            cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of
            the CEN-complex, while BMI-1 is transiently co-localized with the
            centromeric region in interphase
  JOURNAL   Genes Cells 9 (2), 105-120 (2004)
   PUBMED   15009096
REFERENCE   9  (bases 1 to 1354)
  AUTHORS   Saffery,R., Sumer,H., Hassan,S., Wong,L.H., Craig,J.M.,
            Todokoro,K., Anderson,M., Stafford,A. and Choo,K.H.
  TITLE     Transcription within a functional human centromere
  JOURNAL   Mol Cell 12 (2), 509-516 (2003)
   PUBMED   14536089
REFERENCE   10 (bases 1 to 1354)
  AUTHORS   Sugata,N., Li,S., Earnshaw,W.C., Yen,T.J., Yoda,K., Masumoto,H.,
            Munekata,E., Warburton,P.E. and Todokoro,K.
  TITLE     Human CENP-H multimers colocalize with CENP-A and CENP-C at active
            centromere--kinetochore complexes
  JOURNAL   Hum Mol Genet 9 (19), 2919-2926 (2000)
   PUBMED   11092768
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022107.8.
            
            On Nov 26, 2018 this sequence version replaced NM_022909.3.
            
            Summary: Centromere and kinetochore proteins play a critical role
            in centromere structure, kinetochore formation, and sister
            chromatid separation. The protein encoded by this gene colocalizes
            with inner kinetochore plate proteins CENP-A and CENP-C in both
            interphase and metaphase. It localizes outside of centromeric
            heterochromatin, where CENP-B is localized, and inside the
            kinetochore corona, where CENP-E is localized during prometaphase.
            It is thought that this protein can bind to itself, as well as to
            CENP-A, CENP-B or CENP-C. Multimers of the protein localize
            constitutively to the inner kinetochore plate and play an important
            role in the organization and function of the active
            centromere-kinetochore complex. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.400574.1, BC012024.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000283006.7/ ENSP00000283006.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               AC022107.8         95041-95226
            187-242             AC022107.8         97253-97308
            243-291             AC022107.8         100105-100153
            292-366             AC022107.8         101175-101249
            367-423             AC022107.8         102511-102567
            424-487             AC022107.8         107964-108027
            488-539             AC022107.8         108377-108428
            540-703             AC022107.8         113654-113817
            704-1354            AC022107.8         115165-115815
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.2"
     gene            1..1354
                     /gene="CENPH"
                     /note="centromere protein H"
                     /db_xref="GeneID:64946"
                     /db_xref="HGNC:HGNC:17268"
                     /db_xref="MIM:605607"
     exon            1..186
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..796
                     /gene="CENPH"
                     /note="kinetochore protein CENP-H; interphase centromere
                     complex protein 35"
                     /codon_start=1
                     /product="centromere protein H"
                     /protein_id="NP_075060.1"
                     /db_xref="CCDS:CCDS3998.1"
                     /db_xref="GeneID:64946"
                     /db_xref="HGNC:HGNC:17268"
                     /db_xref="MIM:605607"
                     /translation="MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLL
                     RLRAQTKQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLA
                     LDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKK
                     RLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQN
                     LILGSKVNWAEDPALKEIVLQLEKNVDMM"
     exon            187..242
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            243..291
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            292..366
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            367..423
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            424..487
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            488..539
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            540..703
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     exon            704..1354
                     /gene="CENPH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      795..800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPH"
                     /note="hexamer: AATAAG"
     polyA_site      822
                     /gene="CENPH"
     regulatory      1335..1340
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPH"
                     /note="hexamer: AATAAA"
     polyA_site      1354
                     /gene="CENPH"
                     /note="major polyA site"
ORIGIN      
        1 cttttctgag cgcgtttgcc tgttgagtgg tagcctttcc cctcaaccag caatggagga
       61 gcagccccag atgcaagacg ccgacgagcc cgcggactcc ggaggggaag gccgggcagg
      121 cgggccaccg caggtcgccg gcgcccaggc ggcgtgcagc gaggaccgca tgaccctgct
      181 cctcaggctg agagcacaga caaaacaaca actcttagaa tataaatcaa tggttgatgc
      241 aagtgaagaa aaaactccag aacaaattat gcaagaaaag caaatcgaag ctaaaattga
      301 agacctggaa aatgaaattg aagaggtaaa agttgctttt gagataaaaa agcttgcatt
      361 agacaggatg agactttcaa ctgcacttaa aaaaaacctg gagaaaatta gcagacagtc
      421 tagtgtgctc atggataaca tgaaacacct attagagcta aataaattaa taatgaaatc
      481 acagcaggaa tcttgggatt tagaggaaaa actgcttgat attagaaaga agagattgca
      541 attaaaacaa gcttcagaaa gtaagctttt agaaatacag actgaaaaga acaaacagaa
      601 gattgatttg gacagtatgg aaaactcaga gaggataaag atcatacgac aaaacctaca
      661 gatggagata aaaattacta ctgttattca acatgtgttc cagaacctta ttttggggag
      721 taaagtcaat tgggcagagg atcctgccct taaggaaatt gttctgcagc ttgagaagaa
      781 tgttgacatg atgtaataag aattcatttc tgacatattt tacatttctg gcaatctcaa
      841 ctcttatttg gaatacttct gtgcatttgt ctgtccaccg taattttaga aaagcatatc
      901 cataacgttt acagttgtag tacagttgtg gttagttatt tgtagtggga ttgaaagtaa
      961 tttttttctt tttatatttc tatatttagt ttgttttttt gttgttgttg ttttttgaga
     1021 tggagtctcg ctttgttgcc cagactggag ggcagtggcg cgatctcggc tcactgcaac
     1081 ctctgcctcc cgggttcaag cagttctgcc tcagcctccc aagtagctgt gactaaaggt
     1141 gcacgccgcc atgcccagct aattttttgt attttagtag agacggggtt tcaccgtgtt
     1201 gcccaggctg ctctcagaac tcctgagctc aggcagtcca ccgcctcggc ctaccgaagt
     1261 gctaggatta cagacgtaag ccaccgagcc tggtctagtt tgcatttttt ttctatcagt
     1321 tttataagtt aagaaataaa aggaattaat gtta
//
