LOCUS       NM_016343              10290 bp    mRNA    linear   PRI 22-JAN-2024
DEFINITION  Homo sapiens centromere protein F (CENPF), mRNA.
ACCESSION   NM_016343 NM_005196
VERSION     NM_016343.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Xu,Z., Xu,D., You,Z. and Tian,W.
  TITLE     CENPF Upregulation is Associated with Immunosuppressive Status and
            Poor Clinical Outcomes in Lung Adenocarcinoma Validated by qRT-PCR
  JOURNAL   Comb Chem High Throughput Screen 27 (1), 78-89 (2024)
   PUBMED   37287300
  REMARK    GeneRIF: CENPF Upregulation is Associated with Immunosuppressive
            Status and Poor Clinical Outcomes in Lung Adenocarcinoma Validated
            by qRT-PCR.
REFERENCE   2  (bases 1 to 10290)
  AUTHORS   Misceo,D., Senaratne,L.D.S., Mero,I.L., Sundaram,A.Y.M.,
            Bjornstad,P.M., Szczaluba,K., Gasperowicz,P., Kamien,B.,
            Nedregaard,B., Holmgren,A., Stromme,P. and Frengen,E.
  TITLE     Novel Loss of Function Variants in CENPF Including a Large
            Intragenic Deletion in Patients with Stromme Syndrome
  JOURNAL   Genes (Basel) 14 (11), 1985 (2023)
   PUBMED   38002928
  REMARK    GeneRIF: Novel Loss of Function Variants in CENPF Including a Large
            Intragenic Deletion in Patients with Stromme Syndrome.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10290)
  AUTHORS   Zhou,S., Cao,C. and Hu,J.
  TITLE     Long Non-Coding RNA Small Nucleolar RNA Host Gene 4 Induced by
            Transcription Factor SP1 Promoted the Progression of Nasopharyngeal
            Carcinoma Through Modulating microRNA-510-5p/Centromere Protein F
            Axis
  JOURNAL   Biochem Genet 61 (5), 1967-1986 (2023)
   PUBMED   36899270
  REMARK    GeneRIF: Long Non-Coding RNA Small Nucleolar RNA Host Gene 4
            Induced by Transcription Factor SP1 Promoted the Progression of
            Nasopharyngeal Carcinoma Through Modulating
            microRNA-510-5p/Centromere Protein F Axis.
REFERENCE   4  (bases 1 to 10290)
  AUTHORS   Sun,J., Lu,P., Guan,S. and Liu,S.
  TITLE     [Heterogeneity analysis of pancreatic cancer and identification of
            molecular subtypes of tumor cells based on CEACAM5, LGALS1 and
            CENPF gene expression]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (9), 1567-1576 (2023)
   PUBMED   37814871
  REMARK    GeneRIF: [Heterogeneity analysis of pancreatic cancer and
            identification of molecular subtypes of tumor cells based on
            CEACAM5, LGALS1 and CENPF gene expression].
REFERENCE   5  (bases 1 to 10290)
  AUTHORS   Cao,Z., Zeng,L., Wang,Z., Wen,X. and Zhang,J.
  TITLE     Integrated pan-cancer analysis of centromere protein F and
            experimental verification of its role and clinical significance in
            cholangiocarcinoma
  JOURNAL   Funct Integr Genomics 23 (2), 190 (2023)
   PUBMED   37247093
  REMARK    GeneRIF: Integrated pan-cancer analysis of centromere protein F and
            experimental verification of its role and clinical significance in
            cholangiocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10290)
  AUTHORS   Zhu,X., Mancini,M.A., Chang,K.H., Liu,C.Y., Chen,C.F., Shan,B.,
            Jones,D., Yang-Feng,T.L. and Lee,W.H.
  TITLE     Characterization of a novel 350-kilodalton nuclear phosphoprotein
            that is specifically involved in mitotic-phase progression
  JOURNAL   Mol Cell Biol 15 (9), 5017-5029 (1995)
   PUBMED   7651420
REFERENCE   7  (bases 1 to 10290)
  AUTHORS   Zhu,X., Chang,K.H., He,D., Mancini,M.A., Brinkley,W.R. and Lee,W.H.
  TITLE     The C terminus of mitosin is essential for its nuclear
            localization, centromere/kinetochore targeting, and dimerization
  JOURNAL   J Biol Chem 270 (33), 19545-19550 (1995)
   PUBMED   7642639
REFERENCE   8  (bases 1 to 10290)
  AUTHORS   Liao,H., Winkfein,R.J., Mack,G., Rattner,J.B. and Yen,T.J.
  TITLE     CENP-F is a protein of the nuclear matrix that assembles onto
            kinetochores at late G2 and is rapidly degraded after mitosis
  JOURNAL   J Cell Biol 130 (3), 507-518 (1995)
   PUBMED   7542657
REFERENCE   9  (bases 1 to 10290)
  AUTHORS   Li,Q., Ke,Y., Kapp,J.A., Fertig,N., Medsger,T.A. Jr. and Joshi,H.C.
  TITLE     A novel cell-cycle-dependent 350-kDa nuclear protein: C-terminal
            domain sufficient for nuclear localization
  JOURNAL   Biochem Biophys Res Commun 212 (1), 220-228 (1995)
   PUBMED   7612011
REFERENCE   10 (bases 1 to 10290)
  AUTHORS   Ho,S.K.L., Leung,L.T., Luk,H.M. and Lo,I.F.M.
  TITLE     Stromme Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH,
            Gripp KW and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36375007
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU662228.1, U30872.1,
            BU155312.1, CD654009.1, BG680819.1 and BQ774943.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_016343.3.
            
            Summary: This gene encodes a protein that associates with the
            centromere-kinetochore complex. The protein is a component of the
            nuclear matrix during the G2 phase of interphase. In late G2 the
            protein associates with the kinetochore and maintains this
            association through early anaphase. It localizes to the spindle
            midzone and the intracellular bridge in late anaphase and
            telophase, respectively, and is thought to be subsequently
            degraded. The localization of this protein suggests that it may
            play a role in chromosome segregation during mitotis. It is thought
            to form either a homodimer or heterodimer. Autoantibodies against
            this protein have been found in patients with cancer or graft
            versus host disease. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U30872.1, ERR3218370.511781.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366955.8/ ENSP00000355922.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-649               BU662228.1         34-682
            650-1992            U30872.1           554-1896
            1993-2007           BU155312.1         305-319
            2008-5306           U30872.1           1909-5207
            5307-5316           CD654009.1         502-511
            5317-8990           U30872.1           5218-8891
            8991-8999           BG680819.1         531-539
            9000-9481           U30872.1           8901-9382
            9482-9491           BQ774943.1         332-341             c
            9492-10290          U30872.1           9393-10191
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41"
     gene            1..10290
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /note="centromere protein F"
                     /db_xref="GeneID:1063"
                     /db_xref="HGNC:HGNC:1857"
                     /db_xref="MIM:600236"
     exon            1..127
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            128..330
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..9513
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /note="mitosin; CENP-F kinetochore protein; AH antigen;
                     cell-cycle-dependent 350K nuclear protein; centromere
                     protein F, 350/400kDa; kinetochore protein CENPF"
                     /codon_start=1
                     /product="centromere protein F"
                     /protein_id="NP_057427.3"
                     /db_xref="CCDS:CCDS31023.1"
                     /db_xref="GeneID:1063"
                     /db_xref="HGNC:HGNC:1857"
                     /db_xref="MIM:600236"
                     /translation="MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSL
                     EAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEG
                     QLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYS
                     GSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFS
                     WQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSS
                     PHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEK
                     VLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEK
                     EKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLE
                     NNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAE
                     LKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQ
                     RDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKS
                     ENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNL
                     HNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQ
                     LLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFA
                     NIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEEL
                     VQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQ
                     LLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEEL
                     TQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCE
                     ETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTK
                     LAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEV
                     NDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLD
                     SYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLK
                     DCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTE
                     LNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHP
                     VSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQ
                     TYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSS
                     FYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEEL
                     ESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQ
                     QKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRN
                     ESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQS
                     PDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENL
                     RLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDL
                     SEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDV
                     ENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTS
                     ERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELL
                     QTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESES
                     LQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADE
                     KKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQ
                     IEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEI
                     QIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKL
                     RARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKG
                     EVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENIL
                     QEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLEL
                     EKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLV
                     SKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENE
                     LELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLL
                     EEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLHEAEKKHQA
                     LLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEEL
                     KKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKT
                     GTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVA
                     HLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRG
                     PTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYIL
                     RRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGT
                     ILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ"
     exon            331..527
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            528..649
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            650..741
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            742..1033
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1236
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1362
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1491
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1614
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1750
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..5154
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            5155..7998
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            7999..8151
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            8152..8328
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            8329..8490
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            8491..8653
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            8654..9130
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            9131..9309
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     exon            9310..10290
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9785..9790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /note="hexamer: ATTAAA"
     polyA_site      9811
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /note="major polyA site"
     regulatory      10262..10267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
                     /note="hexamer: AATAAA"
     polyA_site      10290
                     /gene="CENPF"
                     /gene_synonym="CENF; CILD31; hcp-1; PRO1779; STROMS"
ORIGIN      
        1 agaagcgggc gaattgggca ccggtggcgg ctgcgggcag tttgaattag actctgggct
       61 ccagcccgcc gaagccgcgc cagaactgta ctctccgaga ggtcgttttc ccgtccccga
      121 gagcaagttt atttacaaat gttggagtaa taaagaaggc agaacaaaat gagctgggct
      181 ttggaagaat ggaaagaagg gctgcctaca agagctcttc agaaaattca agagcttgaa
      241 ggacagcttg acaaactgaa gaaggaaaag cagcaaaggc agtttcagct tgacagtctc
      301 gaggctgcgc tgcagaagca aaaacagaag gttgaaaatg aaaaaaccga gggtacaaac
      361 ctgaaaaggg agaatcaaag attgatggaa atatgtgaaa gtctggagaa aactaagcag
      421 aagatttctc atgaacttca agtcaaggag tcacaagtga atttccagga aggacaactg
      481 aattcaggca aaaaacaaat agaaaaactg gaacaggaac ttaaaaggtg taaatctgag
      541 cttgaaagaa gccaacaagc tgcgcagtct gcagatgtct ctctgaatcc atgcaataca
      601 ccacaaaaaa tttttacaac tccactaaca ccaagtcaat attatagtgg ttccaagtat
      661 gaagatctaa aagaaaaata taataaagag gttgaagaac gaaaaagatt agaggcagag
      721 gttaaagcct tgcaggctaa aaaagcaagc cagactcttc cacaagccac catgaatcac
      781 cgcgacattg cccggcatca ggcttcatca tctgtgttct catggcagca agagaagacc
      841 ccaagtcatc tttcatctaa ttctcaaaga actccaatta ggagagattt ctctgcatct
      901 tacttttctg gggaacaaga ggtgactcca agtcgatcaa ctttgcaaat agggaaaaga
      961 gatgctaata gcagtttctt tgacaattct agcagtcctc atcttttgga tcaattaaaa
     1021 gcgcagaatc aagagctaag aaacaagatt aatgagttgg aactacgcct gcaaggacat
     1081 gaaaaagaaa tgaaaggcca agtgaataag tttcaagaac tccaactcca actggagaaa
     1141 gcaaaagtgg aattaattga aaaagagaaa gttttgaaca aatgtaggga tgaactagtg
     1201 agaacaacag cacaatacga ccaggcgtca accaagtata ctgcattgga acaaaaactg
     1261 aaaaaattga cggaagattt gagttgtcag cgacaaaatg cagaaagtgc cagatgttct
     1321 ctggaacaga aaattaagga aaaagaaaag gagtttcaag aggagctctc ccgtcaacag
     1381 cgttctttcc aaacactgga ccaggagtgc atccagatga aggccagact cacccaggag
     1441 ttacagcaag ccaagaatat gcacaacgtc ctgcaggctg aactggataa actcacatca
     1501 gtaaagcaac agctagaaaa caatttggaa gagtttaagc aaaagttgtg cagagctgaa
     1561 caggcgttcc aggcgagtca gatcaaggag aatgagctga ggagaagcat ggaggaaatg
     1621 aagaaggaaa acaacctcct taagagtcac tctgagcaaa aggccagaga agtctgccac
     1681 ctggaggcag aactcaagaa catcaaacag tgtttaaatc agagccagaa ttttgcagaa
     1741 gaaatgaaag cgaagaatac ctctcaggaa accatgttaa gagatcttca agaaaaaata
     1801 aatcagcaag aaaactcctt gactttagaa aaactgaagc ttgctgtggc tgatctggaa
     1861 aagcagcgag attgttctca agaccttttg aagaaaagag aacatcacat tgaacaactt
     1921 aatgataagt taagcaagac agagaaagag tccaaagcct tgctgagtgc tttagagtta
     1981 aaaaagaaag aatatgaaga attgaaagaa gagaaaactc tgttttcttg ttggaaaagt
     2041 gaaaacgaaa aacttttaac tcagatggaa tcagaaaagg aaaacttgca gagtaaaatt
     2101 aatcacttgg aaacttgtct gaagacacag caaataaaaa gtcatgaata caacgagaga
     2161 gtaagaacgc tggagatgga cagagaaaac ctaagtgtcg agatcagaaa ccttcacaac
     2221 gtgttagaca gtaagtcagt ggaggtagag acccagaaac tagcttatat ggagctacag
     2281 cagaaagctg agttctcaga tcagaaacat cagaaggaaa tagaaaatat gtgtttgaag
     2341 acttctcagc ttactgggca agttgaagat ctagaacaca agcttcagtt actgtcaaat
     2401 gaaataatgg acaaagaccg gtgttaccaa gacttgcatg ccgaatatga gagcctcagg
     2461 gatctgctaa aatccaaaga tgcttctctg gtgacaaatg aagatcatca gagaagtctt
     2521 ttggcttttg atcagcagcc tgccatgcat cattcctttg caaatataat tggagaacaa
     2581 ggaagcatgc cttcagagag gagtgaatgt cgtttagaag cagaccaaag tccgaaaaat
     2641 tctgccatcc tacaaaatag agttgattca cttgaatttt cattagagtc tcaaaaacag
     2701 atgaactcag acctgcaaaa gcagtgtgaa gagttggtgc aaatcaaagg agaaatagaa
     2761 gaaaatctca tgaaagcaga acagatgcat caaagttttg tggctgaaac aagtcagcgc
     2821 attagtaagt tacaggaaga cacttctgct caccagaatg ttgttgctga aaccttaagt
     2881 gcccttgaga acaaggaaaa agagctgcaa cttttaaatg ataaggtaga aactgagcag
     2941 gcagagattc aagaattaaa aaagagcaac catctacttg aagactctct aaaggagcta
     3001 caacttttat ccgaaaccct aagcttggag aagaaagaaa tgagttccat catttctcta
     3061 aataaaaggg aaattgaaga gctgacccaa gagaatggga ctcttaagga aattaatgca
     3121 tccttaaatc aagagaagat gaacttaatc cagaaaagtg agagttttgc aaactatata
     3181 gatgaaaggg agaaaagcat ttcagagtta tctgatcagt acaagcaaga aaaacttatt
     3241 ttactacaaa gatgtgaaga aaccggaaat gcatatgagg atcttagtca aaaatacaaa
     3301 gcagcacagg aaaagaattc taaattagaa tgcttgctaa atgaatgcac tagtctttgt
     3361 gaaaatagga aaaatgagtt ggaacagcta aaggaagcat ttgcaaagga acaccaagaa
     3421 ttcttaacaa aattagcatt tgctgaagaa agaaatcaga atctgatgct agagttggag
     3481 acagtgcagc aagctctgag atctgagatg acagataacc aaaacaattc taagagcgag
     3541 gctggtggtt taaagcaaga aatcatgact ttaaaggaag aacaaaacaa aatgcaaaag
     3601 gaagttaatg acttattaca agagaatgaa cagctgatga aggtaatgaa gactaaacat
     3661 gaatgtcaaa atctagaatc agaaccaatt aggaactctg tgaaagaaag agagagtgag
     3721 agaaatcaat gtaattttaa acctcagatg gatcttgaag ttaaagaaat ttctctagat
     3781 agttataatg cgcagttggt gcaattagaa gctatgctaa gaaataagga attaaaactt
     3841 caggaaagtg agaaggagaa ggagtgcctg cagcatgaat tacagacaat tagaggagat
     3901 cttgaaacca gcaatttgca agacatgcag tcacaagaaa ttagtggcct taaagactgt
     3961 gaaatagatg cggaagaaaa gtatatttca gggcctcatg agttgtcaac aagtcaaaac
     4021 gacaatgcac accttcagtg ctctctgcaa acaacaatga acaagctgaa tgagctagag
     4081 aaaatatgtg aaatactgca ggctgaaaag tatgaactcg taactgagct gaatgattca
     4141 aggtcagaat gtatcacagc aactaggaaa atggcagaag aggtagggaa actactaaat
     4201 gaagttaaaa tattaaatga tgacagtggt cttctccatg gtgagttagt ggaagacata
     4261 ccaggaggtg aatttggtga acaaccaaat gaacagcacc ctgtgtcttt ggctccattg
     4321 gacgagagta attcctacga gcacttgaca ttgtcagaca aagaagttca aatgcacttt
     4381 gccgaattgc aagagaaatt cttatcttta caaagtgaac acaaaatttt acatgatcag
     4441 cactgtcaga tgagctctaa aatgtcagag ctgcagacct atgttgactc attaaaggcc
     4501 gaaaatttgg tcttgtcaac gaatctgaga aactttcaag gtgacttggt gaaggagatg
     4561 cagctgggct tggaggaggg gctcgttcca tccctgtcat cctcttgtgt gcctgacagc
     4621 tctagtctta gcagtttggg agactcctcc ttttacagag ctcttttaga acagacagga
     4681 gatatgtctc ttttgagtaa tttagaaggg gctgtttcag caaaccagtg cagtgtagat
     4741 gaagtatttt gcagcagtct gcaggaggag aatctgacca ggaaagaaac cccttcggcc
     4801 ccagcgaagg gtgttgaaga gcttgagtcc ctctgtgagg tgtaccggca gtccctcgag
     4861 aagctagaag agaaaatgga aagtcaaggg attatgaaaa ataaggaaat tcaagagctc
     4921 gagcagttat taagttctga aaggcaagag cttgactgcc ttaggaagca gtatttgtca
     4981 gaaaatgaac agtggcaaca gaagctgaca agcgtgactc tggagatgga gtccaagttg
     5041 gcggcagaaa agaaacagac ggaacaactg tcacttgagc tggaagtagc acgactccag
     5101 ctacaaggtc tggacttaag ttctcggtct ttgcttggca tcgacacaga agatgctatt
     5161 caaggccgaa atgagagctg tgacatatca aaagaacata cttcagaaac tacagaaaga
     5221 acaccaaagc atgatgttca tcagatttgt gataaagatg ctcagcagga cctcaatcta
     5281 gacattgaga aaataactga gactggtgca gtgaaaccca caggagagtg ctctggggaa
     5341 cagtccccag ataccaatta tgagcctcca ggggaagata aaacccaggg ctcttcagaa
     5401 tgcatttctg aattgtcatt ttctggtcct aatgctttgg tacctatgga tttcctgggg
     5461 aatcaggaag atatccataa tcttcaactg cgggtaaaag agacatcaaa tgagaatttg
     5521 agattacttc atgtgataga ggaccgtgac agaaaagttg aaagtttgct aaatgaaatg
     5581 aaagaattag actcaaaact ccatttacag gaggtacaac taatgaccaa aattgaagca
     5641 tgcatagaat tggaaaaaat agttggggaa cttaagaaag aaaactcaga tttaagtgaa
     5701 aaattggaat atttttcttg tgatcaccag gagttactcc agagagtaga aacttctgaa
     5761 ggcctcaatt ctgatttaga aatgcatgca gataaatcat cacgtgaaga tattggagat
     5821 aatgtggcca aggtgaatga cagctggaag gagagatttc ttgatgtgga aaatgagctg
     5881 agtaggatca gatcggagaa agctagcatt gagcatgaag ccctctacct ggaggctgac
     5941 ttagaggtag ttcaaacaga gaagctatgt ttagaaaaag acaatgaaaa taagcagaag
     6001 gttattgtct gccttgaaga agaactctca gtggtcacaa gtgagagaaa ccagcttcgt
     6061 ggagaattag atactatgtc aaaaaaaacc acggcactgg atcagttgtc tgaaaaaatg
     6121 aaggagaaaa cacaagagct tgagtctcat caaagtgagt gtctccattg cattcaggtg
     6181 gcagaggcag aggtgaagga aaagacggaa ctccttcaga ctttgtcctc tgatgtgagt
     6241 gagctgttaa aagacaaaac tcatctccag gaaaagctgc agagtttgga aaaggactca
     6301 caggcactgt ctttgacaaa atgtgagctg gaaaaccaaa ttgcacaact gaataaagag
     6361 aaagaattgc ttgtcaagga atctgaaagc ctgcaggcca gactgagtga atcagattat
     6421 gaaaagctga atgtctccaa ggccttggag gccgcactgg tggagaaagg tgagttcgca
     6481 ttgaggctga gctcaacaca ggaggaagtg catcagctga gaagaggcat cgagaaactg
     6541 agagttcgca ttgaggccga tgaaaagaag cagctgcaca tcgcagagaa actgaaagaa
     6601 cgcgagcggg agaatgattc acttaaggat aaagttgaga accttgaaag ggaattgcag
     6661 atgtcagaag aaaaccagga gctagtgatt cttgatgccg agaattccaa agcagaagta
     6721 gagactctaa aaacacaaat agaagagatg gccagaagcc tgaaagtttt tgaattagac
     6781 cttgtcacgt taaggtctga aaaagaaaat ctgacaaaac aaatacaaga aaaacaaggt
     6841 cagttgtcag aactagacaa gttactctct tcatttaaaa gtctgttaga agaaaaggag
     6901 caagcagaga tacagatcaa agaagaatct aaaactgcag tggagatgct tcagaatcag
     6961 ttaaaggagc taaatgaggc agtagcagcc ttgtgtggtg accaagaaat tatgaaggcc
     7021 acagaacaga gtctagaccc accaatagag gaagagcatc agctgagaaa tagcattgaa
     7081 aagctgagag cccgcctaga agctgatgaa aagaagcagc tctgtgtctt acaacaactg
     7141 aaggaaagtg agcatcatgc agatttactt aagggtagag tggagaacct tgaaagagag
     7201 ctagagatag ccaggacaaa ccaagagcat gcagctcttg aggcagagaa ttccaaagga
     7261 gaggtagaga ccctaaaagc aaaaatagaa gggatgaccc aaagtctgag aggtctggaa
     7321 ttagatgttg ttactataag gtcagaaaaa gaaaatctga caaatgaatt acaaaaagag
     7381 caagagcgaa tatctgaatt agaaataata aattcatcat ttgaaaatat tttgcaagaa
     7441 aaagagcaag agaaagtaca gatgaaagaa aaatcaagca ctgccatgga gatgcttcaa
     7501 acacaattaa aagagctcaa tgagagagtg gcagccctgc ataatgacca agaagcctgt
     7561 aaggccaaag agcagaatct tagtagtcaa gtagagtgtc ttgaacttga gaaggctcag
     7621 ttgctacaag gccttgatga ggccaaaaat aattatattg ttttgcaatc ttcagtgaat
     7681 ggcctcattc aagaagtaga agatggcaag cagaaactgg agaagaagga tgaagaaatc
     7741 agtagactga aaaatcaaat tcaagaccaa gagcagcttg tctctaaact gtcccaggtg
     7801 gaaggagagc accaactttg gaaggagcaa aacttagaac tgagaaatct gacagtggaa
     7861 ttggagcaga agatccaagt gctacaatcc aaaaatgcct ctttgcagga cacattagaa
     7921 gtgctgcaga gttcttacaa gaatctagag aatgagcttg aattgacaaa aatggacaaa
     7981 atgtcctttg ttgaaaaagt aaacaaaatg actgcaaagg aaactgagct gcagagggaa
     8041 atgcatgaga tggcacagaa aacagcagag ctgcaagaag aactcagtgg agagaaaaat
     8101 aggctagctg gagagttgca gttactgttg gaagaaataa agagcagcaa agatcaattg
     8161 aaggagctca cactagaaaa tagtgaattg aagaagagcc tagattgcat gcacaaagac
     8221 caggtggaaa aggaagggaa agtgagagag gaaatagctg aatatcagct acggcttcat
     8281 gaagctgaaa agaaacacca ggctttgctt ttggacacaa acaaacagta tgaagtagaa
     8341 atccagacat accgagagaa attgacttct aaagaagaat gtctcagttc acagaagctg
     8401 gagatagacc ttttaaagtc tagtaaagaa gagctcaata attcattgaa agctactact
     8461 cagattttgg aagaattgaa gaaaaccaag atggacaatc taaaatatgt aaatcagttg
     8521 aagaaggaaa atgaacgtgc ccaggggaaa atgaagttgt tgatcaaatc ctgtaaacag
     8581 ctggaagagg aaaaggagat actgcagaaa gaactctctc aacttcaagc tgcacaggag
     8641 aagcagaaaa caggtactgt tatggatacc aaggtcgatg aattaacaac tgagatcaaa
     8701 gaactgaaag aaactcttga agaaaaaacc aaggaggcag atgaatactt ggataagtac
     8761 tgttccttgc ttataagcca tgaaaagtta gagaaagcta aagagatgtt agagacacaa
     8821 gtggcccatc tgtgttcaca gcaatctaaa caagattccc gagggtctcc tttgctaggt
     8881 ccagttgttc caggaccatc tccaatccct tctgttactg aaaagaggtt atcatctggc
     8941 caaaataaag cttcaggcaa gaggcaaaga tccagtggaa tatgggagaa tggtagagga
     9001 ccaacacctg ctaccccaga gagcttttct aaaaaaagca agaaagcagt catgagtggt
     9061 attcaccctg cagaagacac ggaaggtact gagtttgagc cagagggact tccagaagtt
     9121 gtaaagaaag ggtttgctga catcccgaca ggaaagacta gcccatatat cctgcgaaga
     9181 acaaccatgg caactcggac cagcccccgc ctggctgcac agaagttagc gctatcccca
     9241 ctgagtctcg gcaaagaaaa tcttgcagag tcctccaaac caacagctgg tggcagcaga
     9301 tcacaaaagg tcaaagttgc tcagcggagc ccagtagatt caggcaccat cctccgagaa
     9361 cccaccacga aatccgtccc agtcaataat cttcctgaga gaagtccgac tgacagcccc
     9421 agagagggcc tgagggtcaa gcgaggccga cttgtcccca gccccaaagc tggactggag
     9481 tccaacggca gtgagaactg taaggtccag tgaaggcact ttgtgtgtca gtacccctgg
     9541 gaggtgccag tcattgaata gataaggctg tgcctacagg acttctcttt agtcagggca
     9601 tgctttatta gtgaggagaa aacaattcct tagaagtctt aaatatattg tactctttag
     9661 atctcccatg tgtaggtatt gaaaaagttt ggaagcactg atcacctgtt agcattgcca
     9721 ttcctctact gcaatgtaaa tagtataaag ctatgtatat aaagcttttt ggtaatatgt
     9781 tacaattaaa atgacaagca ctatatcaca atctctgttt gtatgtgggt tttacactaa
     9841 aaaaatgcaa aacacatttt attcttctaa ttaacagctc ctaggaaaat gtagactttt
     9901 gctttatgat attctatctg tagtatgagg catggaatag ttttgtatcg ggaatttctc
     9961 agagctgagt aaaatgaagg aaaagcatgt tatgtgtttt taaggaaaat gtgcacacat
    10021 atacatgtag gagtgtttat ctttctctta caatctgttt tagacatctt tgcttatgaa
    10081 acctgtacat atgtgtgtgt gggtatgtgt ttatttccag tgagggctgc aggcttccta
    10141 gaggtgtgct ataccatgcg tctgtcgttg tgcttttttc tgtttttaga ccaatttttt
    10201 acagttcttt ggtaagcatt gtcgtatctg gtgatggatt aacatatagc ctttgttttc
    10261 taataaaata gtcgccttcg ttttctgtaa
//
