LOCUS       NM_001813               8541 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens centromere protein E (CENPE), transcript variant 1,
            mRNA.
ACCESSION   NM_001813
VERSION     NM_001813.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Lakshmi,R.B., Nayak,P., Raz,L., Sarkar,A., Saroha,A., Kumari,P.,
            Nair,V.M., Kombarakkaran,D.P., Sajana,S., M,G. S, Agasti,S.S.,
            Paul,R., Ben-David,U. and Manna,T.K.
  TITLE     CKAP5 stabilizes CENP-E at kinetochores by regulating
            microtubule-chromosome attachments
  JOURNAL   EMBO Rep 25 (4), 1909-1935 (2024)
   PUBMED   38424231
  REMARK    GeneRIF: CKAP5 stabilizes CENP-E at kinetochores by regulating
            microtubule-chromosome attachments.
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Wu,J., Raas,M.W.D., Alcaraz,P.S., Vos,H.R., Tromer,E.C., Snel,B.
            and Kops,G.J.P.L.
  TITLE     A farnesyl-dependent structural role for CENP-E in expansion of the
            fibrous corona
  JOURNAL   J Cell Biol 223 (1) (2024)
   PUBMED   37934467
  REMARK    GeneRIF: A farnesyl-dependent structural role for CENP-E in
            expansion of the fibrous corona.
REFERENCE   3  (bases 1 to 8541)
  AUTHORS   Eibes,S., Rajendraprasad,G., Guasch-Boldu,C., Kubat,M.,
            Steblyanko,Y. and Barisic,M.
  TITLE     CENP-E activation by Aurora A and B controls kinetochore fibrous
            corona disassembly
  JOURNAL   Nat Commun 14 (1), 5317 (2023)
   PUBMED   37658044
  REMARK    GeneRIF: CENP-E activation by Aurora A and B controls kinetochore
            fibrous corona disassembly.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8541)
  AUTHORS   Shi,K., Zhu,X., Wu,J., Chen,Y., Zhang,J. and Sun,X.
  TITLE     Centromere protein E as a novel biomarker and potential therapeutic
            target for retinoblastoma
  JOURNAL   Bioengineered 12 (1), 5950-5970 (2021)
   PUBMED   34482803
  REMARK    GeneRIF: Centromere protein E as a novel biomarker and potential
            therapeutic target for retinoblastoma.
REFERENCE   5  (bases 1 to 8541)
  AUTHORS   Cilluffo,D., Chiavetta,R.F., Bivona,S., Contino,F., Coronnello,C.,
            Feo,S., Di Leonardo,A. and Barra,V.
  TITLE     Transcriptomic Changes Following Partial Depletion of CENP-E in
            Normal Human Fibroblasts
  JOURNAL   Genes (Basel) 12 (9), 1322 (2021)
   PUBMED   34573304
  REMARK    GeneRIF: Transcriptomic Changes Following Partial Depletion of
            CENP-E in Normal Human Fibroblasts.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8541)
  AUTHORS   Wood,K.W., Sakowicz,R., Goldstein,L.S. and Cleveland,D.W.
  TITLE     CENP-E is a plus end-directed kinetochore motor required for
            metaphase chromosome alignment
  JOURNAL   Cell 91 (3), 357-366 (1997)
   PUBMED   9363944
REFERENCE   7  (bases 1 to 8541)
  AUTHORS   Thrower,D.A., Jordan,M.A., Schaar,B.T., Yen,T.J. and Wilson,L.
  TITLE     Mitotic HeLa cells contain a CENP-E-associated minus end-directed
            microtubule motor
  JOURNAL   EMBO J 14 (5), 918-926 (1995)
   PUBMED   7889940
REFERENCE   8  (bases 1 to 8541)
  AUTHORS   Testa,J.R., Zhou,J.Y., Bell,D.W. and Yen,T.J.
  TITLE     Chromosomal localization of the genes encoding the kinetochore
            proteins CENPE and CENPF to human chromosomes 4q24-->q25 and
            1q32-->q41, respectively, by fluorescence in situ hybridization
  JOURNAL   Genomics 23 (3), 691-693 (1994)
   PUBMED   7851898
REFERENCE   9  (bases 1 to 8541)
  AUTHORS   Yen,T.J., Li,G., Schaar,B.T., Szilak,I. and Cleveland,D.W.
  TITLE     CENP-E is a putative kinetochore motor that accumulates just before
            mitosis
  JOURNAL   Nature 359 (6395), 536-539 (1992)
   PUBMED   1406971
REFERENCE   10 (bases 1 to 8541)
  AUTHORS   Yen,T.J., Compton,D.A., Wise,D., Zinkowski,R.P., Brinkley,B.R.,
            Earnshaw,W.C. and Cleveland,D.W.
  TITLE     CENP-E, a novel human centromere-associated protein required for
            progression from metaphase to anaphase
  JOURNAL   EMBO J 10 (5), 1245-1254 (1991)
   PUBMED   2022189
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU100312.1, Z15005.1,
            BQ434767.1, BQ231878.1, CD644410.1, AC079919.5, AL596934.1,
            AW499901.1, CN418651.1, AA210858.1, AA210728.1 and BQ447425.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 12, 2019 this sequence version replaced NM_001813.2.
            
            Summary: Centrosome-associated protein E (CENPE) is a kinesin-like
            motor protein that accumulates in the G2 phase of the cell cycle.
            Unlike other centrosome-associated proteins, it is not present
            during interphase and first appears at the centromere region of
            chromosomes during prometaphase. This protein is required for
            stable spindle microtubule capture at kinetochores which is a
            necessary step in chromosome alignment during prometaphase. This
            protein also couples chromosome position to microtubule
            depolymerizing activity. Alternative splicing results in multiple
            transcript variants encoding distinct protein isoforms. [provided
            by RefSeq, Nov 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265148.9/ ENSP00000265148.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-266               AU100312.1         8-273
            267-899             Z15005.1           333-965
            900-1186            BQ434767.1         382-668
            1187-1719           Z15005.1           1253-1785
            1720-2050           BQ231878.1         22-352              c
            2051-2727           Z15005.1           2117-2793
            2728-3123           CD644410.1         306-701
            3124-3912           Z15005.1           3190-3978
            3913-4158           AC079919.5         47376-47621         c
            4159-4626           Z15005.1           4225-4692
            4627-5102           AL596934.1         102-577
            5103-5648           Z15005.1           5169-5714
            5649-5864           AW499901.1         371-586
            5865-6045           Z15005.1           5931-6111
            6046-6292           CN418651.1         155-401
            6293-6542           AA210858.1         110-359
            6543-6592           Z15005.1           6495-6544
            6593-7035           AA210728.1         1-443               c
            7036-8305           Z15005.1           6988-8257
            8306-8541           BQ447425.1         24-259              c
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q24"
     gene            1..8541
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /note="centromere protein E"
                     /db_xref="GeneID:1062"
                     /db_xref="HGNC:HGNC:1856"
                     /db_xref="MIM:117143"
     exon            1..80
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..8130
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; Centromere autoantigen E (312kD); kinesin
                     family member 10; centromere-associated protein E;
                     kinesin-related protein CENPE; protein phosphatase 1,
                     regulatory subunit 61; centromere protein E, 312kDa;
                     kinesin-7"
                     /codon_start=1
                     /product="centromere-associated protein E isoform 1
                     precursor"
                     /protein_id="NP_001804.2"
                     /db_xref="CCDS:CCDS34042.1"
                     /db_xref="GeneID:1062"
                     /db_xref="HGNC:HGNC:1856"
                     /db_xref="MIM:117143"
                     /translation="MAEEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGS
                     KSFNFDRVFHGNETTKNVYEEIAAPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSEDH
                     LGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNR
                     NVYVADLTEEVVYTSEMALKWITKGEKSRHYGETKMNQRSSRSHTIFRMILESREKGE
                     PSNCEGSVKVSHLNLVDLAGSERAAQTGAAGVRLKEGCNINRSLFILGQVIKKLSDGQ
                     VGGFINYRDSKLTRILQNSLGGNAKTRIICTITPVSFDETLTALQFASTAKYMKNTPY
                     VNEVSTDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQNE
                     KIENLTRMLVTSSSLTLQQELKAKRKRRVTWCLGKINKMKNSNYADQFNIPTNITTKT
                     HKLSINLLREIDESVCSESDVFSNTLDTLSEIEWNPATKLLNQENIESELNSLRADYD
                     NLVLDYEQLRTEKEEMELKLKEKNDLDEFEALERKTKKDQEMQLIHEISNLKNLVKHA
                     EVYNQDLENELSSKVELLREKEDQIKKLQEYIDSQKLENIKMDLSYSLESIEDPKQMK
                     QTLFDAETVALDAKRESAFLRSENLELKEKMKELATTYKQMENDIQLYQSQLEAKKKM
                     QVDLEKELQSAFNEITKLTSLIDGKVPKDLLCNLELEGKITDLQKELNKEVEENEALR
                     EEVILLSELKSLPSEVERLRKEIQDKSEELHIITSEKDKLFSEVVHKESRVQGLLEEI
                     GKTKDDLATTQSNYKSTDQEFQNFKTLHMDFEQKYKMVLEENERMNQEIVNLSKEAQK
                     FDSSLGALKTELSYKTQELQEKTREVQERLNEMEQLKEQLENRDSTLQTVEREKTLIT
                     EKLQQTLEEVKTLTQEKDDLKQLQESLQIERDQLKSDIHDTVNMNIDTQEQLRNALES
                     LKQHQETINTLKSKISEEVSRNLHMEENTGETKDEFQQKMVGIDKKQDLEAKNTQTLT
                     ADVKDNEIIEQQRKIFSLIQEKNELQQMLESVIAEKEQLKTDLKENIEMTIENQEELR
                     LLGDELKKQQEIVAQEKNHAIKKEGELSRTCDRLAEVEEKLKEKSQQLQEKQQQLLNV
                     QEEMSEMQKKINEIENLKNELKNKELTLEHMETERLELAQKLNENYEEVKSITKERKV
                     LKELQKSFETERDHLRGYIREIEATGLQTKEELKIAHIHLKEHQETIDELRRSVSEKT
                     AQIINTQDLEKSHTKLQEEIPVLHEEQELLPNVKEVSETQETMNELELLTEQSTTKDS
                     TTLARIEMERLRLNEKFQESQEEIKSLTKERDNLKTIKEALEVKHDQLKEHIRETLAK
                     IQESQSKQEQSLNMKEKDNETTKIVSEMEQFKPKDSALLRIEIEMLGLSKRLQESHDE
                     MKSVAKEKDDLQRLQEVLQSESDQLKENIKEIVAKHLETEEELKVAHCCLKEQEETIN
                     ELRVNLSEKETEISTIQKQLEAINDKLQNKIQEIYEKEEQFNIKQISEVQEKVNELKQ
                     FKEHRKAKDSALQSIESKMLELTNRLQESQEEIQIMIKEKEEMKRVQEALQIERDQLK
                     ENTKEIVAKMKESQEKEYQFLKMTAVNETQEKMCEIEHLKEQFETQKLNLENIETENI
                     RLTQILHENLEEMRSVTKERDDLRSVEETLKVERDQLKENLRETITRDLEKQEELKIV
                     HMHLKEHQETIDKLRGIVSEKTNEISNMQKDLEHSNDALKAQDLKIQEELRIAHMHLK
                     EQQETIDKLRGIVSEKTDKLSNMQKDLENSNAKLQEKIQELKANEHQLITLKKDVNET
                     QKKVSEMEQLKKQIKDQSLTLSKLEIENLNLAQKLHENLEEMKSVMKERDNLRRVEET
                     LKLERDQLKESLQETKARDLEIQQELKTARMLSKEHKETVDKLREKISEKTIQISDIQ
                     KDLDKSKDELQKKIQELQKKELQLLRVKEDVNMSHKKINEMEQLKKQFEAQNLSMQSV
                     RMDNFQLTKKLHESLEEIRIVAKERDELRRIKESLKMERDQFIATLREMIARDRQNHQ
                     VKPEKRLLSDGQQHLTESLREKCSRIKELLKRYSEMDDHYECLNRLSLDLEKEIEFQK
                     ELSMRVKANLSLPYLQTKHIEKLFTANQRCSMEFHRIMKKLKYVLSYVTKIKEEQHES
                     INKFEMDFIDEVEKQKELLIKIQHLQQDCDVPSRELRDLKLNQNMDLHIEEILKDFSE
                     SEFPSIKTEFQQVLSNRKEMTQFLEEWLNTRFDIEKLKNGIQKENDRICQVNNFFNNR
                     IIAIMNESTEFEERSATISKEWEQDLKSLKEKNEKLFKNYQTLKTSLASGAQVNPTTQ
                     DNKNPHVTSRATQLTTEKIRELENSLHEAKESAMHKESKIIKMQKELEVTNDIIAKLQ
                     AKVHESNKCLEKTKETIQVLQDKVALGAKPYKEEIEDLKMKLVKIDLEKMKNAKEFEK
                     EISATKATVEYQKEVIRLLRENLRRSQQAQDTSVISEHTDPQPSNKPLTCGGGSGIVQ
                     NTKALILKSEHIRLEKEISKLKQQNEQLIKQKNELLSNNQHLSNEVKTWKERTLKREA
                     HKQVTCENSPKSPKVTGTASKKKQITPSQCKERNLQDPVPKESPKSCFFDSRSKSLPS
                     PHPVRYFDNSSLGLCPEVQNAGAESVDSQPGPWHASSGKDVPECKTQ"
     exon            81..172
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            173..262
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            263..381
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            382..501
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            502..583
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            584..651
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            652..717
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            718..769
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            770..857
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            858..987
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1107
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1266
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1414
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1503
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1671
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1746
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..1866
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..1989
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2155
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..2310
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2625
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2898
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            2899..3057
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            3058..3261
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3420
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3711
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            3712..3867
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..4158
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4437
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4596
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            4597..4881
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            4882..5169
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            5170..5328
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            5329..5487
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            5488..5778
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            5779..5937
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            5938..6228
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            6229..6327
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            6328..6546
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            6547..6744
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            6745..6948
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            6949..7167
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            7168..7353
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            7354..7466
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            7467..7564
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            7565..7748
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            7749..8035
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     exon            8036..8541
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8390..8395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /note="hexamer: AATAAA"
     polyA_site      8409
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
     regulatory      8519..8524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /note="hexamer: AATAAA"
     polyA_site      8541
                     /gene="CENPE"
                     /gene_synonym="CENP-E; KIF10; MCPH13; PPP1R61"
                     /note="major polyA site"
ORIGIN      
        1 ctgggaccag ttcagcctga taggatggcg gaggaaggag ccgtggccgt ctgcgtgcga
       61 gtgcggccgc tgaacagcag agaagaatca cttggagaaa ctgcccaagt ttactggaaa
      121 actgacaata atgtcattta tcaagttgat ggaagtaaat ccttcaattt tgatcgtgtc
      181 tttcatggta atgaaactac caaaaatgtg tatgaagaaa tagcagcacc aatcatcgat
      241 tctgccatac aaggctacaa tggtactata tttgcctatg gacagactgc ttcaggaaaa
      301 acatatacca tgatgggttc agaagatcat ttgggagtta tacccagggc aattcatgac
      361 attttccaaa aaattaagaa gtttcctgat agggaatttc tcttacgtgt atcttacatg
      421 gaaatataca atgaaaccat tacagattta ctctgtggca ctcaaaaaat gaaaccttta
      481 attattcgag aagatgtcaa taggaatgtg tatgttgctg atctcacaga agaagttgta
      541 tatacatcag aaatggcttt gaaatggatt acaaagggag aaaagagcag gcattatgga
      601 gaaacaaaaa tgaatcaaag aagcagtcgt tctcatacca tctttaggat gattttggaa
      661 agcagagaga agggtgaacc ttctaattgt gaaggatctg ttaaggtatc ccatttgaat
      721 ttggttgatc ttgcaggcag tgaaagagct gctcaaacag gcgctgcagg tgtgcggctc
      781 aaggaaggct gtaatataaa tcgaagctta tttattttgg gacaagtgat caagaaactt
      841 agtgatggac aagttggtgg tttcataaat tatcgagata gcaagttaac acgaattctc
      901 cagaattcct tgggaggaaa tgcaaagaca cgtattatct gcacaattac tccagtatct
      961 tttgatgaaa cacttactgc tctccagttt gccagtactg ctaaatatat gaagaatact
     1021 ccttatgtta atgaggtatc aactgatgaa gctctcctga aaaggtatag aaaagaaata
     1081 atggatctta aaaaacaatt agaggaggtt tctttagaga cgcgggctca ggcaatggaa
     1141 aaagaccaat tggcccaact tttggaagaa aaagatttgc ttcagaaagt acagaatgag
     1201 aaaattgaaa acttaacacg gatgctggtg acctcttctt ccctcacgtt gcaacaggaa
     1261 ttaaaggcta aaagaaaacg aagagttact tggtgccttg gcaaaattaa caaaatgaag
     1321 aactcaaact atgcagatca atttaatata ccaacaaata taacaacaaa aacacataag
     1381 ctttctataa atttattacg agaaattgat gaatctgtct gttcagagtc tgatgttttc
     1441 agtaacactc ttgatacatt aagtgagata gaatggaatc cagcaacaaa gctactaaat
     1501 caggagaata tagaaagtga gttgaactca cttcgtgctg actatgataa tctggtatta
     1561 gactatgaac aactacgaac agaaaaagaa gaaatggaat tgaaattaaa agaaaagaat
     1621 gatttggatg aatttgaggc tctagaaaga aaaactaaaa aagatcaaga gatgcaacta
     1681 attcatgaaa tttcgaactt aaagaattta gttaagcatg cagaagtata taatcaagat
     1741 cttgagaatg aactcagttc aaaagtagag ctgcttagag aaaaggaaga ccagattaag
     1801 aagctacagg aatacataga ctctcaaaag ctagaaaata taaaaatgga cttgtcatac
     1861 tcattggaaa gcattgaaga cccaaaacaa atgaagcaga ctctgtttga tgctgaaact
     1921 gtagcccttg atgccaagag agaatcagcc tttcttagaa gtgaaaatct ggagctgaag
     1981 gagaaaatga aagaacttgc aactacatac aagcaaatgg aaaatgatat tcagttatat
     2041 caaagccagt tggaggcaaa aaagaaaatg caagttgatc tggagaaaga attacaatct
     2101 gcttttaatg agataacaaa actcacctcc cttatagatg gcaaagttcc aaaagatttg
     2161 ctctgtaatt tggaattgga aggaaagatt actgatcttc agaaagaact aaataaagaa
     2221 gttgaagaaa atgaagcttt gcgggaagaa gtcattttgc tttcagaatt gaaatcttta
     2281 ccttctgaag tagaaaggct gaggaaagag atacaagaca aatctgaaga gctccatata
     2341 ataacatcag aaaaagataa attgttttct gaagtagttc ataaggagag tagagttcaa
     2401 ggtttacttg aagaaattgg gaaaacaaaa gatgacctag caactacaca gtcgaattat
     2461 aaaagcactg atcaagaatt ccaaaatttc aaaacccttc atatggactt tgagcaaaag
     2521 tataagatgg tccttgagga gaatgagaga atgaatcagg aaatagttaa tctctctaaa
     2581 gaagcccaaa aatttgattc gagtttgggt gctttgaaga ccgagctttc ttacaagacc
     2641 caagaacttc aggagaaaac acgtgaggtt caagaaagac taaatgagat ggaacagctg
     2701 aaggaacaat tagaaaatag agattctacg ctgcaaactg tagaaaggga gaaaacactg
     2761 attactgaga aactgcagca aactttagaa gaagtaaaaa ctttaactca agaaaaagat
     2821 gatctaaaac aactccaaga aagcttgcaa attgagaggg accaactcaa aagtgatatt
     2881 cacgatactg ttaacatgaa tatagatact caagaacaat tacgaaatgc tcttgagtct
     2941 ctgaaacaac atcaagaaac aattaataca ctaaaatcga aaatttctga ggaagtttcc
     3001 aggaatttgc atatggagga aaatacagga gaaactaaag atgaatttca gcaaaagatg
     3061 gttggcatag ataaaaaaca ggatttggaa gctaaaaata cccaaacact aactgcagat
     3121 gttaaggata atgagataat tgagcaacaa aggaagatat tttctttaat acaggagaaa
     3181 aatgaactcc aacaaatgtt agagagtgtt atagcagaaa aggaacaatt gaagactgac
     3241 ctaaaggaaa atattgaaat gaccattgaa aaccaggaag aattaagact tcttggggat
     3301 gaacttaaaa agcaacaaga gatagttgca caagaaaaga accatgccat aaagaaagaa
     3361 ggagagcttt ctaggacctg tgacagactg gcagaagttg aagaaaaact aaaggaaaag
     3421 agccagcaac tccaagaaaa acagcaacaa cttcttaatg tacaagaaga gatgagtgag
     3481 atgcagaaaa agattaatga aatagagaat ttaaagaatg aattaaagaa caaagaattg
     3541 acattggaac atatggaaac agagaggctt gagttggctc agaaacttaa tgaaaattat
     3601 gaggaagtga aatctataac caaagaaaga aaagttctaa aggaattaca gaagtcattt
     3661 gaaacagaga gagaccacct tagaggatat ataagagaaa ttgaagctac aggcctacaa
     3721 accaaagaag aactaaaaat tgctcatatt cacctaaaag aacaccaaga aactattgat
     3781 gaactaagaa gaagcgtatc tgagaagaca gctcaaataa taaatactca ggacttagaa
     3841 aaatcccata ccaaattaca agaagagatc ccagtgcttc atgaggaaca agagttactg
     3901 cctaatgtga aagaagtcag tgagactcag gaaacaatga atgaactgga gttattaaca
     3961 gaacagtcca caaccaagga ctcaacaaca ctggcaagaa tagaaatgga aaggctcagg
     4021 ttgaatgaaa aatttcaaga aagtcaggaa gagataaaat ctctaaccaa ggaaagagac
     4081 aaccttaaaa cgataaaaga agcccttgaa gttaaacatg accagctgaa agaacatatt
     4141 agagaaactt tggctaaaat ccaggagtct caaagcaaac aagaacagtc cttaaatatg
     4201 aaagaaaaag acaatgaaac taccaaaatc gtgagtgaga tggagcaatt caaacccaaa
     4261 gattcagcac tactaaggat agaaatagaa atgctcggat tgtccaaaag acttcaagaa
     4321 agtcatgatg aaatgaaatc tgtagctaag gagaaagatg acctacagag gctgcaagaa
     4381 gttcttcaat ctgaaagtga ccagctcaaa gaaaacataa aagaaattgt agctaaacac
     4441 ctggaaactg aagaggaact taaagttgct cattgttgcc tgaaagaaca agaggaaact
     4501 attaatgagt taagagtgaa tctttcagag aaggaaactg aaatatcaac cattcaaaag
     4561 cagttagaag caatcaatga taaattacag aacaagatcc aagagattta tgagaaagag
     4621 gaacaattta atataaaaca aattagtgag gttcaggaaa aagtgaatga actgaaacaa
     4681 ttcaaggagc atcgcaaagc caaggattca gcactacaaa gtatagaaag taagatgctc
     4741 gagttgacca acagacttca agaaagtcaa gaagaaatac aaattatgat taaggaaaaa
     4801 gaggaaatga aaagagtaca ggaggccctt cagatagaga gagaccaact gaaagaaaac
     4861 actaaagaaa ttgtagctaa aatgaaagaa tctcaagaaa aagaatatca gtttcttaag
     4921 atgacagctg tcaatgagac tcaggagaaa atgtgtgaaa tagaacactt gaaggagcaa
     4981 tttgagaccc agaagttaaa cctggaaaac atagaaacgg agaatataag gttgactcag
     5041 atactacatg aaaaccttga agaaatgaga tctgtaacaa aagaaagaga tgaccttagg
     5101 agtgtggagg agactctcaa agtagagaga gaccagctca aggaaaacct tagagaaact
     5161 ataactagag acctagaaaa acaagaggag ctaaaaattg ttcacatgca tctgaaggag
     5221 caccaagaaa ctattgataa actaagaggg attgtttcag agaaaacaaa tgaaatatca
     5281 aatatgcaaa aggacttaga acactcaaat gatgccttaa aagcacagga tctgaaaata
     5341 caagaggaac taagaattgc tcacatgcat ctgaaagagc agcaggaaac tattgacaaa
     5401 ctcagaggaa ttgtttctga gaagacagat aaactatcaa atatgcaaaa agatttagaa
     5461 aattcaaatg ctaaattaca agaaaagatt caagaactta aggcaaatga acatcaactt
     5521 attacgttaa aaaaagatgt caatgagaca cagaaaaaag tgtctgaaat ggagcaacta
     5581 aagaaacaaa taaaagacca aagcttaact ctgagtaaat tagaaataga gaatttaaat
     5641 ttggctcaga aacttcatga aaaccttgaa gaaatgaaat ctgtaatgaa agaaagagat
     5701 aatctaagaa gagtagagga gacactcaaa ctggagagag accaactcaa ggaaagcctg
     5761 caagaaacca aagctagaga tctggaaata caacaggaac taaaaactgc tcgtatgcta
     5821 tcaaaagaac acaaagaaac tgttgataaa cttagagaaa aaatttcaga aaagacaatt
     5881 caaatttcag acattcaaaa ggatttagat aaatcaaaag atgaattaca gaaaaagatc
     5941 caagaacttc agaaaaaaga acttcaactg cttagagtga aagaagatgt caatatgagt
     6001 cataaaaaaa ttaatgaaat ggaacagttg aagaagcaat ttgaggccca aaacttatct
     6061 atgcaaagtg tgagaatgga taacttccag ttgactaaga aacttcatga aagccttgaa
     6121 gaaataagaa ttgtagctaa agaaagagat gagctaagga ggataaaaga atctctcaaa
     6181 atggaaaggg accaattcat agcaacctta agggaaatga tagctagaga ccgacagaac
     6241 caccaagtaa aacctgaaaa aaggttacta agtgatggac aacagcacct tacggaaagc
     6301 ctgagagaaa agtgctctag aataaaagag cttttgaaga gatactcaga gatggatgat
     6361 cattatgagt gcttgaatag attgtctctt gacttggaga aggaaattga attccaaaaa
     6421 gagctttcaa tgagagttaa agcaaacctc tcacttccct atttacaaac caaacacatt
     6481 gaaaaacttt ttactgcaaa ccagagatgc tccatggaat tccacagaat catgaagaaa
     6541 ctgaagtatg tgttaagcta tgttacaaaa ataaaagaag aacaacatga atccatcaat
     6601 aaatttgaaa tggattttat tgatgaagtg gaaaagcaaa aggaattgct aattaaaata
     6661 cagcaccttc aacaagattg tgatgtacca tccagagaat taagggatct caaattgaac
     6721 cagaatatgg atctacatat tgaggaaatt ctcaaagatt tctcagaaag tgagttccct
     6781 agcataaaga ctgaatttca acaagtacta agtaatagga aagaaatgac acagtttttg
     6841 gaagagtggt taaatactcg ttttgatata gaaaagctta aaaatggcat ccagaaagaa
     6901 aatgatagga tttgtcaagt gaataacttc tttaataaca gaataattgc cataatgaat
     6961 gaatcaacag agtttgagga aagaagtgct accatatcca aagagtggga acaggacctg
     7021 aaatcactga aagagaaaaa tgaaaaacta tttaaaaact accaaacatt gaagacttcc
     7081 ttggcatctg gtgcccaggt taatcctacc acacaagaca ataagaatcc tcatgttaca
     7141 tcaagagcta cacagttaac cacagagaaa attcgagagc tggaaaattc actgcatgaa
     7201 gctaaagaaa gtgctatgca taaggaaagc aagattataa agatgcagaa agaacttgag
     7261 gtgactaatg acataatagc aaaacttcaa gccaaagttc atgaatcaaa taaatgcctt
     7321 gaaaaaacaa aagagacaat tcaagtactt caggacaaag ttgctttagg agctaagcca
     7381 tataaagaag aaattgaaga tctcaaaatg aagcttgtga aaatagacct agagaaaatg
     7441 aaaaatgcca aagaatttga aaaggaaatc agtgctacaa aagccactgt agaatatcaa
     7501 aaggaagtta taaggctatt gagagaaaat ctcagaagaa gtcaacaggc ccaagatacc
     7561 tcagtgatat cagaacatac tgatcctcag ccttcaaata aacccttaac ttgtggaggt
     7621 ggcagcggca ttgtacaaaa cacaaaagct cttattttga aaagtgaaca tataaggcta
     7681 gaaaaagaaa tttctaagtt aaagcagcaa aatgaacagc taataaaaca aaagaatgaa
     7741 ttgttaagca ataatcagca tctttccaat gaggtcaaaa cttggaagga aagaaccctt
     7801 aaaagagagg ctcacaaaca agtaacttgt gagaattctc caaagtctcc taaagtgact
     7861 ggaacagctt ctaaaaagaa acaaattaca ccctctcaat gcaaggaacg gaatttacaa
     7921 gatcctgtgc caaaggaatc accaaaatct tgtttttttg atagccgatc aaagtcttta
     7981 ccatcacctc atccagttcg ctattttgat aactcaagtt taggcctttg tccagaggtg
     8041 caaaatgcag gagcagagag tgtggattct cagccaggtc cttggcacgc ctcctcaggc
     8101 aaggatgtgc ctgagtgcaa aactcagtag actcctcttt gtcacttctc tggagatcca
     8161 gcattcctta tttggaaatg actttgttta tgtgtctatc cctggtaatg atgttgtagt
     8221 gcagcttaat ttcaattcag tctttacttt gccactagag ttgaaagata agggaacagg
     8281 aaatgaatgc attgtggtaa tttagaatgg tgatagcaat accttcttct tgcatatggt
     8341 aatactttta aaagttgaat tgttttattt atttgtatat tttgtaaaga ataaagttat
     8401 tgaaagaaat gtaaagttat ctacatgact tagcatattc caaagcataa tacatacatt
     8461 aatataaaac atcattttat taacaaaatt gtaaatgttt ttaatacctt acacattcaa
     8521 taaatgttta gtagttctga a
//
