LOCUS       NM_001376376            8132 bp    mRNA    linear   PRI 24-JUN-2024
DEFINITION  Homo sapiens CUGBP Elav-like family member 1 (CELF1), transcript
            variant 14, mRNA.
ACCESSION   NM_001376376
VERSION     NM_001376376.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Kikuchi,M., Viet,J., Nagata,K., Sato,M., David,G., Audic,Y.,
            Silverman,M.A., Yamamoto,M., Akatsu,H., Hashizume,Y., Takeda,S.,
            Akamine,S., Miyamoto,T., Uozumi,R., Gotoh,S., Mori,K., Ikeda,M.,
            Paillard,L. and Morihara,T.
  TITLE     Gene-gene functional relationships in Alzheimer's disease: CELF1
            regulates KLC1 alternative splicing
  JOURNAL   Biochem Biophys Res Commun 721, 150025 (2024)
   PUBMED   38768546
  REMARK    GeneRIF: Gene-gene functional relationships in Alzheimer's disease:
            CELF1 regulates KLC1 alternative splicing.
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   David,G., Reboutier,D., Deschamps,S., Mereau,A., Taylor,W.,
            Padilla-Parra,S., Tramier,M., Audic,Y. and Paillard,L.
  TITLE     The RNA-binding proteins CELF1 and ELAVL1 cooperatively control the
            alternative splicing of CD44
  JOURNAL   Biochem Biophys Res Commun 626, 79-84 (2022)
   PUBMED   35973378
  REMARK    GeneRIF: The RNA-binding proteins CELF1 and ELAVL1 cooperatively
            control the alternative splicing of CD44.
REFERENCE   3  (bases 1 to 8132)
  AUTHORS   Zhao,X., Wang,J., Zhu,R., Zhang,J. and Zhang,Y.
  TITLE     DLX6-AS1 activated by H3K4me1 enhanced secondary cisplatin
            resistance of lung squamous cell carcinoma through modulating
            miR-181a-5p/miR-382-5p/CELF1 axis
  JOURNAL   Sci Rep 11 (1), 21014 (2021)
   PUBMED   34697393
  REMARK    GeneRIF: DLX6-AS1 activated by H3K4me1 enhanced secondary cisplatin
            resistance of lung squamous cell carcinoma through modulating
            miR-181a-5p/miR-382-5p/CELF1 axis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8132)
  AUTHORS   Liu,C., Wang,H., Tang,L., Huang,H., Xu,M., Lin,Y., Zhou,L., Ho,L.,
            Lu,J. and Ai,X.
  TITLE     LncRNA BACE1-AS enhances the invasive and metastatic capacity of
            hepatocellular carcinoma cells through mediating miR-377-3p/CELF1
            axis
  JOURNAL   Life Sci 275, 119288 (2021)
   PUBMED   33667514
  REMARK    GeneRIF: LncRNA BACE1-AS enhances the invasive and metastatic
            capacity of hepatocellular carcinoma cells through mediating
            miR-377-3p/CELF1 axis.
REFERENCE   5  (bases 1 to 8132)
  AUTHORS   Jin,H., Liang,G., Yang,L., Liu,L., Wang,B. and Yan,F.
  TITLE     SP1-induced AFAP1-AS1 contributes to proliferation and invasion by
            regulating miR-497-5p/CELF1 pathway in nasopharyngeal carcinoma
  JOURNAL   Hum Cell 34 (2), 491-501 (2021)
   PUBMED   33400247
  REMARK    GeneRIF: SP1-induced AFAP1-AS1 contributes to proliferation and
            invasion by regulating miR-497-5p/CELF1 pathway in nasopharyngeal
            carcinoma.
REFERENCE   6  (bases 1 to 8132)
  AUTHORS   Michalowski,S., Miller,J.W., Urbinati,C.R., Paliouras,M.,
            Swanson,M.S. and Griffith,J.
  TITLE     Visualization of double-stranded RNAs from the myotonic dystrophy
            protein kinase gene and interactions with CUG-binding protein
  JOURNAL   Nucleic Acids Res 27 (17), 3534-3542 (1999)
   PUBMED   10446244
REFERENCE   7  (bases 1 to 8132)
  AUTHORS   Roberts,R., Timchenko,N.A., Miller,J.W., Reddy,S., Caskey,C.T.,
            Swanson,M.S. and Timchenko,L.T.
  TITLE     Altered phosphorylation and intracellular distribution of a (CUG)n
            triplet repeat RNA-binding protein in patients with myotonic
            dystrophy and in myotonin protein kinase knockout mice
  JOURNAL   Proc Natl Acad Sci U S A 94 (24), 13221-13226 (1997)
   PUBMED   9371827
REFERENCE   8  (bases 1 to 8132)
  AUTHORS   Timchenko,L.T., Miller,J.W., Timchenko,N.A., DeVore,D.R.,
            Datar,K.V., Lin,L., Roberts,R., Caskey,C.T. and Swanson,M.S.
  TITLE     Identification of a (CUG)n triplet repeat RNA-binding protein and
            its expression in myotonic dystrophy
  JOURNAL   Nucleic Acids Res 24 (22), 4407-4414 (1996)
   PUBMED   8948631
REFERENCE   9  (bases 1 to 8132)
  AUTHORS   Bhagavati,S., Ghatpande,A. and Leung,B.
  TITLE     Identification of two nuclear proteins which bind to RNA CUG
            repeats: significance for myotonic dystrophy
  JOURNAL   Biochem Biophys Res Commun 228 (1), 55-62 (1996)
   PUBMED   8912635
  REMARK    Erratum:[Biochem Biophys Res Commun. 2008 Jun;370(3):530. Bhagwati,
            S [corrected to Bhagavati, S]]
REFERENCE   10 (bases 1 to 8132)
  AUTHORS   Timchenko,L.T., Timchenko,N.A., Caskey,C.T. and Roberts,R.
  TITLE     Novel proteins with binding specificity for DNA CTG repeats and RNA
            CUG repeats: implications for myotonic dystrophy
  JOURNAL   Hum Mol Genet 5 (1), 115-121 (1996)
   PUBMED   8789448
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090559.7.
            
            Summary: Members of the CELF/BRUNOL protein family contain two
            N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM
            domain, and a divergent segment of 160-230 aa between the second
            and third RRM domains. Members of this protein family regulate
            pre-mRNA alternative splicing and may also be involved in mRNA
            editing, and translation. This gene may play a role in myotonic
            dystrophy type 1 (DM1) via interactions with the dystrophia
            myotonica-protein kinase (DMPK) gene. Alternative splicing results
            in multiple transcript variants encoding different isoforms.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.4687.1,
                                           SRR14038194.1491841.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000687097.1/ ENSP00000508525.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AC090559.7         177465-177605       c
            142-213             AC090559.7         125334-125405       c
            214-365             AC090559.7         123926-124077       c
            366-553             AC090559.7         113310-113497       c
            554-636             AC090559.7         111632-111714       c
            637-685             AC090559.7         111223-111271       c
            686-820             AC090559.7         108862-108996       c
            821-900             AC090559.7         107926-108005       c
            901-1062            AC090559.7         107168-107329       c
            1063-1138           AC090559.7         103350-103425       c
            1139-1267           AC090559.7         101770-101898       c
            1268-1381           AC090559.7         101319-101432       c
            1382-1567           AC090559.7         99809-99994         c
            1568-1711           AC090559.7         97561-97704         c
            1712-8132           AC090559.7         90410-96830         c
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p11.2"
     gene            1..8132
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /note="CUGBP Elav-like family member 1"
                     /db_xref="GeneID:10658"
                     /db_xref="HGNC:HGNC:2549"
                     /db_xref="MIM:601074"
     exon            1..141
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            142..213
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            214..365
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     CDS             295..1839
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /note="isoform 6 is encoded by transcript variant 14; CUG
                     RNA-binding protein; embryo deadenylation element binding
                     protein; nuclear polyadenylated RNA-binding protein,
                     50-kD; bruno-like 2; CUG-BP- and ETR-3-like factor 1;
                     EDEN-BP homolog; bruno-like protein 2; deadenylation
                     factor CUG-BP; RNA-binding protein BRUNOL-2; CUG triplet
                     repeat RNA-binding protein 1; 50 kDa nuclear
                     polyadenylated RNA-binding protein; embryo deadenylation
                     element-binding protein homolog"
                     /codon_start=1
                     /product="CUGBP Elav-like family member 1 isoform 6"
                     /protein_id="NP_001363305.1"
                     /db_xref="CCDS:CCDS81565.1"
                     /db_xref="GeneID:10658"
                     /db_xref="HGNC:HGNC:2549"
                     /db_xref="MIM:601074"
                     /translation="MAAFKLDFLPEMMVDHCSLNSSPVSKKMNGTLDHPDQPDLDAIK
                     MFVGQVPRTWSEKDLRELFEQYGAVYEINVLRDRSQNPPQSKGCCFVTFYTRKAALEA
                     QNALHNMKVLPGMHHPIQMKPADSEKNNAVEDRKLFIGMISKKCTENDIRVMFSSFGQ
                     IEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPMVVKFADTQKD
                     KEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTASSGNLNTLSS
                     LHPMGGLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSAGSSPSSSSS
                     NSVNPIASLGALQTLAGATAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYS
                     GIQQYAAAALPTLYNQNLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFM
                     PFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSK
                     NDSKPY"
     exon            366..553
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            554..636
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            637..685
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            686..820
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            821..900
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1062
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1138
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1267
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1381
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1567
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1711
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..8132
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8113..8118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /note="hexamer: ATTAAA"
     polyA_site      8132
                     /gene="CELF1"
                     /gene_synonym="BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP;
                     hNab50; NAB50; NAPOR"
                     /note="major polyA site"
ORIGIN      
        1 gtgttctgct ctggcggcag cggcagcggc ggcgggacgc ggaggctccc ccgggattcg
       61 gcctcagcag cgaggcggcg gcggcggctg cggaggcgca ggcagcaact gaggcagcgg
      121 caggctcagg tgcagccgct ggctgaacat aacttctttt tttcaaagtt ccctgtgttg
      181 tgaactgagc ttgggtgaag tgaaatcttt gaggaagcta aagctgtgtg tatttgtgga
      241 tcagacaagt gcagcaagtt aatatcattg gcttctgaag gggagagtga ggtgatggct
      301 gcgtttaagt tggatttcct tccagaaatg atggtggatc attgctcttt gaattccagt
      361 cccgtctcaa agaaaatgaa cggcaccctg gaccacccag accaaccaga tcttgatgct
      421 atcaagatgt ttgtgggcca ggttccaagg acctggtctg aaaaggactt gcgggaactc
      481 ttcgaacagt atggtgctgt gtatgaaatc aacgtcctaa gggataggag ccaaaacccg
      541 cctcagagca aagggtgctg ttttgttaca ttttacaccc gtaaagctgc attagaagct
      601 cagaatgctc ttcacaacat gaaagtcctc ccagggatgc atcaccctat acagatgaaa
      661 cctgctgaca gtgagaagaa caatgcagtg gaagacagga agctgtttat tggtatgatt
      721 tccaagaagt gcactgaaaa tgacatccga gtcatgttct cttcgtttgg acagattgaa
      781 gaatgccgga tattgcgggg acctgatggc ctgagccgag gttgtgcatt tgtgactttt
      841 acaacaagag ccatggcaca gacggctatc aaggcaatgc accaagcaca gaccatggag
      901 ggttgctcat cacccatggt ggtaaaattt gctgatacac agaaggacaa agaacagaag
      961 agaatggccc agcagctcca gcagcagatg cagcaaatca gcgcagcatc tgtgtgggga
     1021 aaccttgctg gtctaaatac tcttggaccc cagtatttag cactttattt gcagctcctt
     1081 cagcagactg cctcctctgg gaacctcaac accctgagca gcctccaccc aatgggaggg
     1141 ttgaatgcaa tgcagttaca gaatttggct gcactagctg ctgcagctag tgcagctcag
     1201 aacacaccaa gtggtaccaa tgctctcact acatccagca gtcccctcag cgtgctcact
     1261 agttcagcag ggtcctcacc tagctctagc agcagtaatt ctgtcaaccc catagcctca
     1321 cttggagccc tgcagacatt agctggagca acggctggcc tcaatgttgg ctctttggca
     1381 ggaatggctg ctttaaatgg tggcctgggc agcagtggcc tttccaatgg caccgggagc
     1441 accatggagg ccctcactca ggcctactcg ggtatccagc aatatgctgc tgctgcgctc
     1501 cccactctgt acaaccagaa tcttctgaca cagcagagta ttggtgctgc tggaagccag
     1561 aaggaaggtc cagagggagc caacctgttc atctaccacc tgccccagga gtttggtgat
     1621 caggacctgc tgcagatgtt tatgcccttt gggaatgtcg tgtctgccaa ggttttcata
     1681 gacaagcaga caaacctgag caagtgtttt ggttttgtaa gttacgacaa tcctgtttcg
     1741 gcccaagctg ccatccagtc catgaacggc tttcagattg gcatgaagcg gcttaaagtg
     1801 cagctcaaac gttcgaagaa tgacagcaag ccctactgag cgtgctcccc tctgagactg
     1861 gagtgagagg gtcttctggt aagtggggga ggagcaccct taatgattcg aagccctgag
     1921 gctgtgtgtt gacagccctg gaccctgatc cctgccactc tcgcaggcac agcttgccct
     1981 gaagactcgg ctactgcctt ctgtgggagt ttcgcttcgt acagaggaca agtttgtgct
     2041 ttggtttcca gtgttttact ttggagttta ggtgccatat cctgaggttt tttttgtttt
     2101 tgtttttgtt tctcctttat ttaaagtttg ctgtgtttgt aaccagtgtg tgttgagaag
     2161 gaccaacacc aaaccaccct ggggaagggg gacagggaaa catttaacac agacgatgat
     2221 caggatttgc cccaaaccac cccaagagag aggactgagt ggaacaaaaa agtgaccccc
     2281 agaacctctc ttagtgtgag ggtgggtaga atgagaactg acacctggga gctgtgggga
     2341 gcagagcggc tttggggaag agtggagtga ctagacacct aatgccctgg cagctggaga
     2401 ctcaaactcc tgtacagcta ccttctggga aatagttttt gacacctatt tttcagattc
     2461 ttgtccggaa tttctgctgc tcttttcaaa aaagggcatt aacaattctc tggaaataaa
     2521 gcacctgtta gcctgacata tgcaaaaagc agggcggcat cacccattac gagctccccc
     2581 agccagcagt cagtattgga ttggccttgc ctggctggtg gcagtttggg agaaacagcc
     2641 aaagaggtta gtttatttca acaccaaatt agaccatggc ccatttccaa cgggtctctt
     2701 taaaggcctc tatggaatac attgcctggt ttccttcttt gcagttcacc acagcaagga
     2761 atgtcacctc catcccaagc caccattttc tcatgaaggc aaatccaaga agggcttgca
     2821 gttcttgctg aagggggtac catttgtggg cagagtgatc aataccatct agttggggga
     2881 ggaggagctt atttcttggt gtacttgaat cagaaggtcc ctgcaagcca gtatgcttca
     2941 tttgccagtg gccagaaatt ctcccttgcc tccttgattg aggtgtccca gatgtagtat
     3001 tcccacaggg gtctggcagg cccctcctgt aaccactcca gtcacatttt ctgctcttga
     3061 ggcagaggtg acatcaggac gtttacagcc tccacatgaa ttgagtgttc atttacctca
     3121 gtattaccgt gttcattttt gtcctcgtgc tacagttagc tccctgccgc ctcttgcagg
     3181 tcttacttca gctcttgcct gtgacccaca cagcttctgg gctctgcctc tgctctaaga
     3241 agtgctgtgg gggtagagaa ccaggaagga catgctgttg ggaatgtaca cctgggcaga
     3301 ggtggccctg ttaagatgtg actgtagcca cagccaggga tgggatccct gggtctctgg
     3361 aatctgaaac ctcaacaaat accagtcttg acccctagca gcagagataa gaataaaggg
     3421 gttggtttgc tattaagtca gatgggggct ctctccttgt catgctgtcc ctgtgggtaa
     3481 cagacaggga ttgaccatct tactgttgta caggtagctg gtggtgttgg attatcccat
     3541 tgaagctggg acagctgttc ctttctcata gtgataactt gggtctgttg tcctctaagg
     3601 aacgtgaaac atacacttct taagctgaac cacgtaaact tgaaattctg cgcactggga
     3661 gaaatgtgtc ctgtgtttca aaggataaaa ctgttctaaa ggcttccagg gtcatgatgg
     3721 gattttttaa ataaatgcaa aaaataaaaa taaaaaagaa aaaagaaaac aaaaaaaaaa
     3781 aagaaaaaaa tgctaggttg gggaggcgct gttctgaatc ccaaccggct ggaaccaaga
     3841 atgtcgtttc tatttttata gaagttttat acagctccgg ggtggagaat atttattacc
     3901 taaattatat ctctggaaaa ggccaggatt ttgtagaatc cagaatgtga ttgttataca
     3961 cacagggtgc tgtgtatgat tgaaactact gactttctgt ggcccgtttg cagcccagct
     4021 aacctgccca aggggaaggt gttaatgctg tgaattggca gaggagagag ctgtgctcca
     4081 tagggtgctg ccggtgctgt gctccctagc cttctgttct gtcttcctcc ttagccagaa
     4141 cgccactccc ttcccacatc cccttcttct ctccgccttc ccccatctca ccattatttg
     4201 tccatggatt tgtcctgggc tctgggacct tatgaaaccc ctttcctaat gacataagag
     4261 gccaaggtgc aatccaccca cctttgatac cagaccctga gggcttcata cctgctgatg
     4321 ggttttcttt tctaaaagga atgctcgccc cagcagggtc ctgggctgct tgagcagcca
     4381 gctggtgaga ccatgcactt ctctgttctc tcctccccct gcccagtgag ttagcacagc
     4441 agtagcactg cccttgagca cagttctttc cccaggccaa agatggtttt gtgaagaagc
     4501 tgctccccta caagtctcac gtgtgaaagg gctcagctca gagtggagac tcgctgtcaa
     4561 gtcttaagca atccttttct gttgtgtgaa ggtttcactt acaatgtgtt ttctgctgta
     4621 gttttgtttc ttacttgggt tacatcatga aattgatttg ccttgaatgc agccagacca
     4681 ctgtctcctg ggttcccctt gagtggccag ggcctggatg gaagctaaga gcttggattt
     4741 ctgggaacaa agggccatcc ccataagatt gagcagaaat tctggtcctc ttccctgaaa
     4801 aattttcccc accccagcca gcatcttctg cttccccttg ggttgtgacc agggagtggg
     4861 gagagaggct gaggctctgc tcccctggag accttctctt aggagttggg tgcttcatgg
     4921 aaaatccaag ccttaggttc ccttctgtct ctggcaatta gatgtgttct tggtgtcctc
     4981 catgtccttt ttgacttttc cctgtgtcca ttgttagcat gtgcaaaagt tccctgtcat
     5041 tacccaaccc ctgcccgccc acctcatttc agggctgtac acacagtgag tgttcctgtt
     5101 ctctctctct ttgtttggat gtattgctct gtgtaactca gtgggtctcc ctctttctgg
     5161 tttgtttttt tttctagatt cctgccgttt gttcatcgtt gtgcctaaag catgtcgatg
     5221 tggcgtcaag tacatcgtcc aaatccctgt ctcttcagct tctctgatgc ttgaactctc
     5281 acctttgacc ttgtgttgac ctttgatgct gatgtgtatt tttattatgt ttgtttcttt
     5341 cttcgttttt ttttcttttt ttctttcctt tttttttcct tttgtgctgc caaattggtt
     5401 ttgctagaac gactgctgaa ggggaaatat ttaaacttgc atttgaatat aaaaaaaatc
     5461 tatttttcta gaacttcata agataaccac ttgattttgt gattccaatt ctttgtaatt
     5521 gtcttcagag cagccctact agcacatacc gcgtggtgtt tgtatttctg tgaacacaca
     5581 gccagtccgt ttctaggctt tgtttctctg tgtgcttagt tttaaagaca actttgaagt
     5641 aaacaatgaa ataaaagatg tcactaaaac ctctgaggct cctgagcaca ttttgctgat
     5701 acagtctgtg gggcttgagg agaccgcatg tattgttctt tcttttgttt ttcttctgag
     5761 ttctcaactg cggagagcac ctgaaccccc tttccttttt gaccgcaggc tgcactttgg
     5821 gccccagcca gccctttttc tttttctttt tcttttgtgg ttcttccctg gagcgactct
     5881 ggggagtcct ggatatcccg cctgcccctt cccctcagcc ccatgcttgt tccaacagtc
     5941 tccacagcaa aatgtgatgc tttgattttt ttgttgttgt ttttgttttt gtattgtttt
     6001 tgtgtttttg ttttgaattt tttcctttcc tactaagatt atgcccagaa aaaagttttg
     6061 catgtttcct gctgtttctt cacaccttcg tatatatcac cttcacctct ctgttttcta
     6121 tagtttgtgc aaaaactgat cgatttaaaa gggtttcaaa gaagctgttt taaattgttg
     6181 tagggttgat tatttttttc aagattgtat tgtttaattt tgaagtggca actttctcct
     6241 ctattgccct tagagcgttt gcctgtgcac ttagactgtc acttcgtgtg gcctccaggt
     6301 cttaccgggg cttccgggag gctggctgct ttgctcagag agggtgggaa gggggcctgg
     6361 agagacacga gaagcagagg tagagcctag aaggtggcag caggtgggta agaggcttat
     6421 ttagcacatt aggggcagtg agcacctgga ggaaggaggg cgctcccaat cacccgtagg
     6481 aggccatctg cacaccaagc ggcaattcac ctgctggcgc ttttcctagg tgacaagcac
     6541 aatactacag tcttcacact gtttacagcc ctgggcacca gccacccggc actggctctt
     6601 catcacagct ctgctcttgc ttagctagtg gggtggggga aagggcaggg atttgttttt
     6661 ttaattgggt ggagagccaa acagctactg tccctgggtg ccaagcaagc cagttttttg
     6721 gttccctgag ggaaactgac cctcctctct tgtggcacca tccagcctca gggtcttgga
     6781 gacttgagta agaatgtgag tggaggggga gaggtgagga gaggagcaca gggtggatct
     6841 gtggagggaa gaggttacag ggggaggagc tgatgataga tcccacccag acttaagctg
     6901 ctggtgggtg ggtgagctgg gaagtaggac tgtccaggga agggtggaga gatgtagcta
     6961 ggggctgggg agggggaggt ggaagcgcta ttgagcatcc tccacaccaa ggttgatgaa
     7021 ggaagggatc ccagcagggt ttctgctctg gggctggcag gttgcctggt attatgccca
     7081 aggccgctct gcctggggga aagggcagcc aggcagaggc ccagtgtctg gtaggctgct
     7141 gaatttcctg gaaggggtga ttggatggaa agaggccaga aaccccagcc tgagagactg
     7201 ctgtgcaccc cacagtctga ctgcacagag ccgcctctgt tggcaggagg cactgaggct
     7261 ccccttcctg tgtattgaga agcagtgttt gccaatatat tttgctttca attccaagag
     7321 gagctctggg aaaacctgtg gataaaacca aatgccaaat gttggacgtt gtttcctttt
     7381 ccttttctct ctctgattgt tttaattgtt ctgtggtggt tttaatggat ttgagaccct
     7441 ggagcggcag ctgcctttct gatttccagc tgctttttgt gaataattta aaaagaaaaa
     7501 aaaaaagaaa ctttacattt tggagacaaa cctgtgtgag ttttttattg gtacaaacgt
     7561 tgtatttaac actaggggtt ttgtacagtt ttttgccttt tctactagaa aacaatgtaa
     7621 agtgatttca caatgtgaag agaaaaaaaa attgccacta tgaccaaacg cacagtctgt
     7681 tctgcagcaa caacgggatt caatcaactc agtcgtgatt cagccgtaga aatgcttttc
     7741 ctttatcttg tttgagcttt tcctttcttt cctgttttga tttgcaaaag aaaatgtctt
     7801 ttttgtgtga acttgtgttg tactctgtag aaaattatgg attttacttt aatggtttaa
     7861 aaaaaggcaa ggagagccct cgtcgctttt cttacctaat cacagagttt gtgtagtgaa
     7921 tttaaaaaga aaaaaaaatt gttataagtt tggagcaagg gagtatgtgt ttcaaaggaa
     7981 tctccttcct ttttttgtgt gtttttcctt ttgtcccaat ggggaaccta aatctgtttt
     8041 aattgcacag acacatggac aaaaagtcat tttgtatctg ccaagtgtgg taccttcctt
     8101 tgtttatttg ctattaaact gtttgagaag aa
//
