LOCUS       NM_005747                906 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.
ACCESSION   NM_005747
VERSION     NM_005747.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 906)
  AUTHORS   Parniczky,A., Hegyi,E., Toth,A.Z., Szucs,A., Szentesi,A.,
            Vincze,A., Izbeki,F., Nemeth,B.C., Hegyi,P. and Sahin-Toth,M.
  TITLE     Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B
            (CELA3A and CELA3B) to Assess the Role of Complex Formation between
            Proelastases and Procarboxypeptidases in Chronic Pancreatitis
  JOURNAL   Int J Mol Sci 17 (12), 2148 (2016)
   PUBMED   27999401
  REMARK    GeneRIF: variants affecting amino-acid position 241 in CELA3A and
            CELA3B are not associated with chronic pancreatitis, indicating
            that changes in complex formation between proelastases and
            procarboxypeptidases do not alter pancreatitis risk.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 906)
  AUTHORS   Szabo,A., Pilsak,C., Bence,M., Witt,H. and Sahin-Toth,M.
  TITLE     Complex Formation of Human Proelastases with Procarboxypeptidases
            A1 and A2
  JOURNAL   J Biol Chem 291 (34), 17706-17716 (2016)
   PUBMED   27358403
  REMARK    GeneRIF: Complex Formation of Human Proelastases with
            Procarboxypeptidases A1 and A2.
REFERENCE   4  (bases 1 to 906)
  AUTHORS   Shimada,S., Yamaguchi,K., Takahashi,M. and Ogawa,M.
  TITLE     Pancreatic elastase IIIA and its variants are expressed in
            pancreatic carcinoma cells
  JOURNAL   Int J Mol Med 10 (5), 599-603 (2002)
   PUBMED   12373299
  REMARK    GeneRIF: expression of PE IIIA and its splicing variants in
            pancreatic carcinoma cells
REFERENCE   5  (bases 1 to 906)
  AUTHORS   Shirasu,Y., Takemura,K., Yoshida,H., Sato,Y., Iijima,H.,
            Shimada,Y., Mikayama,T., Ozawa,T., Ikeda,N., Ishida,A. et al.
  TITLE     Molecular cloning of complementary DNA encoding one of the human
            pancreatic protease E isozymes
  JOURNAL   J Biochem 104 (2), 259-264 (1988)
   PUBMED   2460440
REFERENCE   6  (bases 1 to 906)
  AUTHORS   Tani,T., Ohsumi,J., Mita,K. and Takiguchi,Y.
  TITLE     Identification of a novel class of elastase isozyme, human
            pancreatic elastase III, by cDNA and genomic gene cloning
  JOURNAL   J Biol Chem 263 (3), 1231-1239 (1988)
   PUBMED   2826474
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC015103.1, AW583482.1 and
            AK225620.1.
            
            On May 17, 2019 this sequence version replaced NM_005747.4.
            
            Summary: Elastases form a subfamily of serine proteases that
            hydrolyze many proteins in addition to elastin. Humans have six
            elastase genes which encode the structurally similar proteins
            elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase
            3A has little elastolytic activity. Like most of the human
            elastases, elastase 3A is secreted from the pancreas as a zymogen
            and, like other serine proteases such as trypsin, chymotrypsin and
            kallikrein, it has a digestive function in the intestine. Elastase
            3A preferentially cleaves proteins after alanine residues. Elastase
            3A may also function in the intestinal transport and metabolism of
            cholesterol. Both elastase 3A and elastase 3B have been referred to
            as protease E and as elastase 1. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225620.1, BC007028.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000290122.8/ ENSP00000290122.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-739               BC015103.1         6-744
            740-771             AW583482.1         135-166             c
            772-895             BC015103.1         777-900
            896-906             AK225620.1         893-903
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.12"
     gene            1..906
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /note="chymotrypsin like elastase 3A"
                     /db_xref="GeneID:10136"
                     /db_xref="HGNC:HGNC:15944"
                     /db_xref="MIM:618693"
     exon            1..61
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..831
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /EC_number="3.4.21.70"
                     /note="protease E; elastase 1; elastase-3A; elastase IIIA;
                     chymotrypsin like elastase family member 3A; elastase 3A,
                     pancreatic"
                     /codon_start=1
                     /product="chymotrypsin-like elastase family member 3A
                     preproprotein"
                     /protein_id="NP_005738.4"
                     /db_xref="CCDS:CCDS220.1"
                     /db_xref="GeneID:10136"
                     /db_xref="HGNC:HGNC:15944"
                     /db_xref="MIM:618693"
                     /translation="MMLRLLSSLLLVAVASGYGPPSSHSSSRVVHGEDAVPYSWPWQV
                     SLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPI
                     NSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYIT
                     GWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSG
                     GPLNCPTEDGGWQVHGVTSFVSAFGCNFIWKPTVFTRVSAFIDWIEETIASH"
     exon            62..147
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     exon            148..245
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     exon            246..380
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     exon            381..517
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     exon            518..660
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     exon            661..813
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
     exon            814..906
                     /gene="CELA3A"
                     /gene_synonym="ELA3; ELA3A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cctatcatca caaaactcat gatgctccgg ctgctcagtt ccctcctcct tgtggccgtt
       61 gcctcaggct atggcccacc ttcctctcac tcttccagcc gcgttgtcca tggtgaggat
      121 gcggtcccct acagctggcc ctggcaggtt tccctgcagt atgagaaaag tggaagcttc
      181 taccacacgt gtggcggtag cctcatcgcc cccgattggg ttgtgactgc cggccactgc
      241 atctcgaggg atctgaccta ccaggtggtg ttgggtgagt acaaccttgc tgtgaaggag
      301 ggccccgagc aggtgatccc catcaactct gaggagctgt ttgtgcatcc actctggaac
      361 cgctcgtgtg tggcctgtgg caatgacatc gccctcatca agctctcacg cagcgcccag
      421 ctgggagatg ccgtccagct cgcctcactc cctcccgctg gtgacatcct tcccaacaag
      481 acaccctgct acatcaccgg ctggggccgt ctctatacca atgggccact cccagacaag
      541 ctgcagcagg cccggctgcc cgtggtggac tataagcact gctccaggtg gaactggtgg
      601 ggttccaccg tgaagaaaac catggtgtgt gctggagggt acatccgctc cggctgcaac
      661 ggtgactctg gaggacccct caactgcccc acagaggatg gtggctggca ggtccacggt
      721 gtgaccagct ttgtttctgc ctttggctgc aacttcatct ggaagcccac ggtgttcact
      781 cgagtctccg ccttcatcga ctggattgag gagaccatag caagccacta gaaccaaggc
      841 ccagctggca gtgctgatcg atcccacatc ctgaataaag aataaagatc tctcagaaaa
      901 ttccaa
//
