LOCUS       NM_015849                915 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens chymotrypsin like elastase 2B (CELA2B), mRNA.
ACCESSION   NM_015849
VERSION     NM_015849.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 915)
  AUTHORS   Hosgood,H.D. 3rd, Zhang,L., Shen,M., Berndt,S.I., Vermeulen,R.,
            Li,G., Yin,S., Yeager,M., Yuenger,J., Rothman,N., Chanock,S.,
            Smith,M. and Lan,Q.
  TITLE     Association between genetic variants in VEGF, ERCC3 and
            occupational benzene haematotoxicity
  JOURNAL   Occup Environ Med 66 (12), 848-853 (2009)
   PUBMED   19773279
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   3  (bases 1 to 915)
  AUTHORS   Macaulay,I.C., Tijssen,M.R., Thijssen-Timmer,D.C., Gusnanto,A.,
            Steward,M., Burns,P., Langford,C.F., Ellis,P.D., Dudbridge,F.,
            Zwaginga,J.J., Watkins,N.A., van der Schoot,C.E. and Ouwehand,W.H.
  TITLE     Comparative gene expression profiling of in vitro differentiated
            megakaryocytes and erythroblasts identifies novel activatory and
            inhibitory platelet membrane proteins
  JOURNAL   Blood 109 (8), 3260-3269 (2007)
   PUBMED   17192395
REFERENCE   4  (bases 1 to 915)
  AUTHORS   Szepessy,E. and Sahin-Toth,M.
  TITLE     Inactivity of recombinant ELA2B provides a new example of
            evolutionary elastase silencing in humans
  JOURNAL   Pancreatology 6 (1-2), 117-122 (2006)
   PUBMED   16327289
REFERENCE   5  (bases 1 to 915)
  AUTHORS   Fletcher,T.S., Shen,W.F. and Largman,C.
  TITLE     Primary structure of human pancreatic elastase 2 determined by
            sequence analysis of the cloned mRNA
  JOURNAL   Biochemistry 26 (23), 7256-7261 (1987)
   PUBMED   3427074
REFERENCE   6  (bases 1 to 915)
  AUTHORS   Kawashima,I., Tani,T., Shimoda,K. and Takiguchi,Y.
  TITLE     Characterization of pancreatic elastase II cDNAs: two elastase II
            mRNAs are expressed in human pancreas
  JOURNAL   DNA 6 (2), 163-172 (1987)
   PUBMED   3646943
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL512883.5.
            
            On May 17, 2019 this sequence version replaced NM_015849.2.
            
            Summary: Elastases form a subfamily of serine proteases that
            hydrolyze many proteins in addition to elastin. Humans have six
            elastase genes which encode the structurally similar proteins
            elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human
            elastases, elastase 2B is secreted from the pancreas as a zymogen.
            In other species, elastase 2B has been shown to preferentially
            cleave proteins after leucine, methionine, and phenylalanine
            residues. [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: DRR138526.135323.1,
                                           DRR138526.54578.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375910.8/ ENSP00000365075.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                AL512883.5         44494-44555
            63-151              AL512883.5         44847-44935
            152-249             AL512883.5         49488-49585
            250-378             AL512883.5         50655-50783
            379-515             AL512883.5         51654-51790
            516-661             AL512883.5         54291-54436
            662-814             AL512883.5         55675-55827
            815-915             AL512883.5         59685-59785
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.21"
     gene            1..915
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /note="chymotrypsin like elastase 2B"
                     /db_xref="GeneID:51032"
                     /db_xref="HGNC:HGNC:29995"
                     /db_xref="MIM:609444"
     exon            1..62
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..832
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /EC_number="3.4.21.71"
                     /note="elastase 2B; pancreatic elastase IIB (ELA2B);
                     RP11-265F14.2; chymotrypsin like elastase family member
                     2B"
                     /codon_start=1
                     /product="chymotrypsin-like elastase family member 2B
                     preproprotein"
                     /protein_id="NP_056933.3"
                     /db_xref="CCDS:CCDS30605.1"
                     /db_xref="GeneID:51032"
                     /db_xref="HGNC:HGNC:29995"
                     /db_xref="MIM:609444"
                     /translation="MIRTLLLSTLVAGALSCGVSTYAPDMSRMLGGEEARPNSWPWQV
                     SLQYSSNGQWYHTCGGSLIANSWVLTAAHCISSSGIYRVMLGQHNLYVAESGSLAVSV
                     SKIVVHKDWNSDQVSKGNDIALLKLANPVSLTDKIQLACLPPAGTILPNNYPCYVTGW
                     GRLQTNGALPDDLKQGQLLVVDYATCSSSGWWGSTVKTNMICAGGDGVICTCNGDSGG
                     PLNCQASDGRWEVHGIGSLTSVLGCNYYYKPSIFTRVSNYNDWINSVIANN"
     exon            63..151
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     exon            152..249
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     exon            250..378
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     exon            379..515
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     exon            516..661
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     exon            662..814
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     exon            815..915
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      894..899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /note="hexamer: TATAAA"
     polyA_site      915
                     /gene="CELA2B"
                     /gene_synonym="ELA2B"
                     /note="major polyA site"
ORIGIN      
        1 acagaactcc cacggacaca ccatgattag gaccctgctg ctgtccactt tggtggctgg
       61 agccctcagt tgtggggtct ccacttacgc gcctgatatg tctaggatgc ttggaggtga
      121 agaagcgagg cccaacagct ggccctggca ggtctccctg cagtacagct ccaatggcca
      181 gtggtaccac acctgcggag ggtccctgat agccaacagc tgggtcctga cggctgccca
      241 ctgcatcagc tcctccggga tctaccgcgt gatgctgggc cagcataacc tctacgttgc
      301 agagtccggc tcgctggccg tcagtgtctc taagattgtg gtgcacaagg actggaactc
      361 cgaccaggtc tccaaaggga acgacattgc cctgctcaaa ctggctaacc ccgtctccct
      421 caccgacaag atccagctgg cctgcctccc tcctgccggc accattctac ccaacaacta
      481 cccctgctac gtcacgggct ggggaaggct gcagaccaac ggggctctcc ctgatgacct
      541 gaagcagggc cagttgctgg ttgtggacta tgccacctgc tccagctctg gctggtgggg
      601 cagcaccgtg aagacgaata tgatctgtgc tgggggtgat ggcgtgatat gcacctgcaa
      661 cggagactcc ggtgggccgc tgaactgtca ggcatctgac ggccggtggg aggtgcatgg
      721 catcggcagc ctcacgtcgg tccttggttg caactactac tacaagccct ccatcttcac
      781 gcgggtctcc aactacaacg actggatcaa ttcggtgatt gcaaataact aaccaaaaga
      841 agtccctggg actgtttcag acttggaaag gtcacagaag gaaaataata ttatataaag
      901 tgacaactat gcaaa
//
