LOCUS       NP_112190                546 aa            linear   PRI 27-APR-2025
DEFINITION  DNA replication factor Cdt1 [Homo sapiens].
ACCESSION   NP_112190
VERSION     NP_112190.2
DBSOURCE    REFSEQ: accession NM_030928.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 546)
  AUTHORS   Mi,Y., Li,X., Wang,C., Lin,C., Zhao,Z., Yan,X., Shi,P. and Wang,L.
  TITLE     [miR-2110 Affects the Biological Behaviors of Lung Adenocarcinoma
            by Regulating CDT1]
  JOURNAL   Sichuan Da Xue Xue Bao Yi Xue Ban 55 (5), 1202-1209 (2024)
   PUBMED   39507968
  REMARK    GeneRIF: [miR-2110 Affects the Biological Behaviors of Lung
            Adenocarcinoma by Regulating CDT1].
REFERENCE   2  (residues 1 to 546)
  AUTHORS   Moriyama,M., Kanda,T., Midorikawa,Y., Matsumura,H., Masuzaki,R.,
            Nakamura,H., Ogawa,M., Matsuoka,S., Shibata,T., Yamazaki,M.,
            Kuroda,K., Nakayama,H., Higaki,T., Kanemaru,K., Miki,T.,
            Sugitani,M. and Takayama,T.
  TITLE     The proliferation of atypical hepatocytes and CDT1 expression in
            noncancerous tissue are associated with the postoperative
            recurrence of hepatocellular carcinoma
  JOURNAL   Sci Rep 12 (1), 20508 (2022)
   PUBMED   36443564
  REMARK    GeneRIF: The proliferation of atypical hepatocytes and CDT1
            expression in noncancerous tissue are associated with the
            postoperative recurrence of hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 546)
  AUTHORS   Fu,Q., Wang,F., Yang,J., Sun,W., Hu,Z., Xu,L., Chu,H., Wang,X. and
            Zhang,W.
  TITLE     Long non-coding RNA-PCGEM1 contributes to prostate cancer
            progression by sponging microRNA miR-129-5p to enhance chromatin
            licensing and DNA replication factor 1 expression
  JOURNAL   Bioengineered 13 (4), 9411-9424 (2022)
   PUBMED   35412947
  REMARK    GeneRIF: Long non-coding RNA-PCGEM1 contributes to prostate cancer
            progression by sponging microRNA miR-129-5p to enhance chromatin
            licensing and DNA replication factor 1 expression.
REFERENCE   4  (residues 1 to 546)
  AUTHORS   Shi,H.X., Huang,S.W., Luo,W.J., Pan,F., Jin,H.J. and Wei,W.
  TITLE     Elevated expression of CDT1 in childhood acute lymphoblastic
            leukemia promotes cell proliferation, invasion and migration
            through activation of EMT
  JOURNAL   J Biol Regul Homeost Agents 35 (Special Issue on Internal Medicine
            n.1) (2021)
   PUBMED   34348833
  REMARK    GeneRIF: Elevated expression of CDT1 in childhood acute
            lymphoblastic leukemia promotes cell proliferation, invasion and
            migration through activation of EMT.
REFERENCE   5  (residues 1 to 546)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (residues 1 to 546)
  AUTHORS   Wohlschlegel,J.A., Dwyer,B.T., Dhar,S.K., Cvetic,C., Walter,J.C.
            and Dutta,A.
  TITLE     Inhibition of eukaryotic DNA replication by geminin binding to Cdt1
  JOURNAL   Science 290 (5500), 2309-2312 (2000)
   PUBMED   11125146
REFERENCE   7  (residues 1 to 546)
  AUTHORS   Izumi,M., Yanagi,K., Mizuno,T., Yokoi,M., Kawasaki,Y., Moon,K.Y.,
            Hurwitz,J., Yatagai,F. and Hanaoka,F.
  TITLE     The human homolog of Saccharomyces cerevisiae Mcm10 interacts with
            replication factors and dissociates from nuclease-resistant nuclear
            structures in G(2) phase
  JOURNAL   Nucleic Acids Res 28 (23), 4769-4777 (2000)
   PUBMED   11095689
REFERENCE   8  (residues 1 to 546)
  AUTHORS   Whittaker,A.J., Royzman,I. and Orr-Weaver,T.L.
  TITLE     Drosophila double parked: a conserved, essential replication
            protein that colocalizes with the origin recognition complex and
            links DNA replication with mitosis and the down-regulation of S
            phase transcripts
  JOURNAL   Genes Dev 14 (14), 1765-1776 (2000)
   PUBMED   10898791
REFERENCE   9  (residues 1 to 546)
  AUTHORS   Nishitani,H., Lygerou,Z., Nishimoto,T. and Nurse,P.
  TITLE     The Cdt1 protein is required to license DNA for replication in
            fission yeast
  JOURNAL   Nature 404 (6778), 625-628 (2000)
   PUBMED   10766248
REFERENCE   10 (residues 1 to 546)
  AUTHORS   Herbig,U., Marlar,C.A. and Fanning,E.
  TITLE     The Cdc6 nucleotide-binding site regulates its activity in DNA
            replication in human cells
  JOURNAL   Mol Biol Cell 10 (8), 2631-2645 (1999)
   PUBMED   10436018
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB053172.1, AC092384.5 and
            BC000137.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 16, 2008 this sequence version replaced NP_112190.1.
            
            Summary: The protein encoded by this gene is involved in the
            formation of the pre-replication complex that is necessary for DNA
            replication. The encoded protein can bind geminin, which prevents
            replication and may function to prevent this protein from
            initiating replication at inappropriate origins. Phosphorylation of
            this protein by cyclin A-dependent kinases results in degradation
            of the protein. [provided by RefSeq, Mar 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB053172.1, SRR1163655.442740.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301019.9/ ENSP00000301019.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q24.3"
     Protein         1..546
                     /product="DNA replication factor Cdt1"
                     /note="Double parked, Drosophila, homolog of"
                     /calculated_mol_wt=60259
     Region          1..118
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          1..23
                     /region_name="PIP-box K+4 motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          <18..>169
                     /region_name="PRK07003"
                     /note="DNA polymerase III subunit gamma/tau"
                     /db_xref="CDD:235906"
     Site            29
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by MAPK8.
                     /evidence=ECO:0000269|PubMed:21856198; propagated from
                     UniProtKB/Swiss-Prot (Q9H211.3)"
     Site            31
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          68..70
                     /region_name="Cyclin-binding motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     Site            93
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by MAPK8.
                     /evidence=ECO:0000269|PubMed:21856198; propagated from
                     UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          143..165
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          150..190
                     /region_name="Interaction with GMNN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          168..352
                     /region_name="Cdt1_m"
                     /note="The middle winged helix fold of replication
                     licensing factor Cdt1 binds geminin to inhibit binding of
                     the MCM complex to origins of replication and DNA;
                     cd08674"
                     /db_xref="CDD:176571"
     Site            order(168..170,172..173,176..179,182,198,228,287..289,292,
                     295,314,323,327..334)
                     /site_type="active"
                     /note="Cdt1-Geminin inhibitory interaction site [active]"
                     /db_xref="CDD:176571"
     Site            order(169..170,172..173,176..179,181,323,327..334,336)
                     /site_type="active"
                     /note="Cdt1-Geminin permissive interaction site [active]"
                     /db_xref="CDD:176571"
     Site            318
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by MAPK8.
                     /evidence=ECO:0000269|PubMed:21856198,
                     ECO:0007744|PubMed:19690332; propagated from
                     UniProtKB/Swiss-Prot (Q9H211.3)"
     Site            380
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          383..415
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     Site            394
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648; propagated from
                     UniProtKB/Swiss-Prot (Q9H211.3)"
     Region          415..537
                     /region_name="Cdt1_c"
                     /note="The C-terminal fold of replication licensing factor
                     Cdt1 is essential for Cdt1 activity and directly interacts
                     with MCM2-7 helicase; cd08767"
                     /db_xref="CDD:176572"
     Site            order(429,433,469..470,474,496,500,504,511,513,516,534,
                     537)
                     /site_type="active"
                     /note="putative Cdt1-MCM complex binding site [active]"
                     /db_xref="CDD:176572"
     Region          451..546
                     /region_name="Interaction with LRWD1.
                     /evidence=ECO:0000269|PubMed:22645314"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H211.3)"
     CDS             1..546
                     /gene="CDT1"
                     /gene_synonym="DUP; RIS2"
                     /coded_by="NM_030928.4:44..1684"
                     /db_xref="CCDS:CCDS32510.1"
                     /db_xref="GeneID:81620"
                     /db_xref="HGNC:HGNC:24576"
                     /db_xref="MIM:605525"
ORIGIN      
        1 meqrrvtdff arrrpgppri appklacrtp sparpalrap asatsgsrkr arppaapgrd
       61 qarpparrrl rlsvdevssp stpeapdipa cpspgqkikk stpaagqpph ltsaqdqdti
      121 selasclqra relgarvral kasaqdages ctpeaegrpe epcgekapay qrfhalaqpg
      181 lpglvlpyky qvlaemfrsm dtivgmlhnr setptfakvq rgvqdmmrrr feecnvgqik
      241 tvypasyrfr qersvptfkd gtrrsdyqlt ieplleqead gaapqltasr llqrrqifsq
      301 klvehvkehh kaflaslspa mvvpedqltr whprfnvdev pdiepaalpq ppatekltta
      361 qevlararnl isprmekals qlalrsaaps spgsprpalp atppatppaa spsalkgvsq
      421 dllerirake aqkqlaqmtr cpeqeqrlqr lerlpelarv lrsvfvserk palsmevaca
      481 rmvgscctim spgemekhll llsellpdwl slhrirtdty vkldkaadla hitarlahqt
      541 raeegl
//
