LOCUS       NM_000389               2117 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens cyclin dependent kinase inhibitor 1A (CDKN1A),
            transcript variant 1, mRNA.
ACCESSION   NM_000389
VERSION     NM_000389.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Hu,F., Zhao,L., Wang,J., Li,X., Xue,Z., Ma,Y., Zheng,M., Chen,C.,
            Tong,M., Guo,X., Li,H., Jin,H., Xie,Q., Zhang,X., Huang,C. and
            Huang,H.
  TITLE     TRIM40 interacts with ROCK1 directly and inhibits colorectal cancer
            cell proliferation through the c-Myc/p21 axis
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (8), 119855 (2024)
   PUBMED   39357549
  REMARK    GeneRIF: TRIM40 interacts with ROCK1 directly and inhibits
            colorectal cancer cell proliferation through the c-Myc/p21 axis.
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   de Azevedo,S.S.D., Ribeiro-Alves,M., Cortes,F.H., Delatorre,E.,
            Hoagland,B., Villela,L.M., Grinsztejn,B., Veloso,V.G.,
            Morgado,M.G., Souza,T.M.L. and Bello,G.
  TITLE     HIV-1 controllers exhibit an enhanced antiretroviral innate state
            characterised by overexpression of p21 and MCPIP1 and silencing of
            ERVK-6 RNA expression
  JOURNAL   Mem Inst Oswaldo Cruz 119, e240071 (2024)
   PUBMED   39292108
  REMARK    GeneRIF: HIV-1 controllers exhibit an enhanced antiretroviral
            innate state characterised by overexpression of p21 and MCPIP1 and
            silencing of ERVK-6 RNA expression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2117)
  AUTHORS   White-Gilbertson,S., Lu,P., Saatci,O., Sahin,O., Delaney,J.R.,
            Ogretmen,B. and Voelkel-Johnson,C.
  TITLE     Transcriptome analysis of polyploid giant cancer cells and their
            progeny reveals a functional role for p21 in polyploidization and
            depolyploidization
  JOURNAL   J Biol Chem 300 (4), 107136 (2024)
   PUBMED   38447798
  REMARK    GeneRIF: Transcriptome analysis of polyploid giant cancer cells and
            their progeny reveals a functional role for p21 in polyploidization
            and depolyploidization.
REFERENCE   4  (bases 1 to 2117)
  AUTHORS   Shuai,Y., Zhang,H., Liu,C., Wang,J., Jiang,Y., Sun,J., Gao,X.,
            Bo,X., Xiao,X., Liao,X., Huang,C., Chen,H. and Jiang,G.
  TITLE     CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine
            modification of p21 mRNA and promote bladder cancer progression
  JOURNAL   Cell Death Dis 15 (1), 9 (2024)
   PUBMED   38182571
  REMARK    GeneRIF: CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine
            modification of p21 mRNA and promote bladder cancer progression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2117)
  AUTHORS   Manousakis,E., Miralles,C.M., Esquerda,M.G. and Wright,R.H.G.
  TITLE     CDKN1A/p21 in Breast Cancer: Part of the Problem, or Part of the
            Solution?
  JOURNAL   Int J Mol Sci 24 (24), 17488 (2023)
   PUBMED   38139316
  REMARK    GeneRIF: CDKN1A/p21 in Breast Cancer: Part of the Problem, or Part
            of the Solution?
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2117)
  AUTHORS   Kim,K.M., Cho,H. and Kim,Y.K.
  TITLE     The upstream open reading frame of cyclin-dependent kinase
            inhibitor 1A mRNA negatively regulates translation of the
            downstream main open reading frame
  JOURNAL   Biochem Biophys Res Commun 424 (3), 469-475 (2012)
   PUBMED   22771799
  REMARK    GeneRIF: These findings provide biological insights into the
            possible role of nonsense-mediated mRNA decay in diverse biological
            pathways mediated by CDKN1A.
REFERENCE   7  (bases 1 to 2117)
  AUTHORS   Kearsey,J.M., Coates,P.J., Prescott,A.R., Warbrick,E. and Hall,P.A.
  TITLE     Gadd45 is a nuclear cell cycle regulated protein which interacts
            with p21Cip1
  JOURNAL   Oncogene 11 (9), 1675-1683 (1995)
   PUBMED   7478594
REFERENCE   8  (bases 1 to 2117)
  AUTHORS   Hall,M., Bates,S. and Peters,G.
  TITLE     Evidence for different modes of action of cyclin-dependent kinase
            inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to
            cyclins
  JOURNAL   Oncogene 11 (8), 1581-1588 (1995)
   PUBMED   7478582
REFERENCE   9  (bases 1 to 2117)
  AUTHORS   Mousses,S., Ozcelik,H., Lee,P.D., Malkin,D., Bull,S.B. and
            Andrulis,I.L.
  TITLE     Two variants of the CIP1/WAF1 gene occur together and are
            associated with human cancer
  JOURNAL   Hum Mol Genet 4 (6), 1089-1092 (1995)
   PUBMED   7655464
REFERENCE   10 (bases 1 to 2117)
  AUTHORS   Demetrick,D.J., Matsumoto,S., Hannon,G.J., Okamoto,K., Xiong,Y.,
            Zhang,H. and Beach,D.H.
  TITLE     Chromosomal mapping of the genes for the human cell cycle proteins
            cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3)
  JOURNAL   Cytogenet Cell Genet 69 (3-4), 190-192 (1995)
   PUBMED   7698009
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z85996.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_000389.4.
            
            Summary: This gene encodes a potent cyclin-dependent kinase
            inhibitor. The encoded protein binds to and inhibits the activity
            of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4
            complexes, and thus functions as a regulator of cell cycle
            progression at G1. The expression of this gene is tightly
            controlled by the tumor suppressor protein p53, through which this
            protein mediates the p53-dependent cell cycle G1 phase arrest in
            response to a variety of stress stimuli. This protein can interact
            with proliferating cell nuclear antigen, a DNA polymerase accessory
            factor, and plays a regulatory role in S phase DNA replication and
            DNA damage repair. This protein was reported to be specifically
            cleaved by CASP3-like caspases, which thus leads to a dramatic
            activation of cyclin-dependent kinase2, and may be instrumental in
            the execution of apoptosis following caspase activation. Mice that
            lack this gene have the ability to regenerate damaged or missing
            tissue. Multiple alternatively spliced variants have been found for
            this gene. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) differs in the 5' UTR, lacks
            an in-frame portion of the 5' coding region, and initiates
            translation at a downstream start codon, compared to variant 3. The
            resulting isoform (1) has a shorter N-terminus, compared to isoform
            2. Variants 1, 2, 4, 5, and 10 all encode the same isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.421140.1, BC013967.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000244741.10/ ENSP00000244741.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                Z85996.1           3213-3297
            86-535              Z85996.1           8596-9045
            536-2117            Z85996.1           10250-11831
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.2"
     gene            1..2117
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /note="cyclin dependent kinase inhibitor 1A"
                     /db_xref="GeneID:1026"
                     /db_xref="HGNC:HGNC:1784"
                     /db_xref="MIM:116899"
     exon            1..85
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            86..535
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..585
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     melanoma differentiation associated protein 6;
                     CDK-interaction protein 1; wild-type p53-activated
                     fragment 1; DNA synthesis inhibitor; cyclin-dependent
                     kinase inhibitor 1; CDK-interacting protein 1;
                     cyclin-dependent kinase inhibitor 1A (p21, Cip1)"
                     /codon_start=1
                     /product="cyclin-dependent kinase inhibitor 1 isoform 1"
                     /protein_id="NP_000380.1"
                     /db_xref="CCDS:CCDS4824.1"
                     /db_xref="GeneID:1026"
                     /db_xref="HGNC:HGNC:1784"
                     /db_xref="MIM:116899"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQE
                     ARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALL
                     QGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFS
                     KRKP"
     exon            536..2117
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2094..2099
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /note="hexamer: AATAAA"
     polyA_site      2117
                     /gene="CDKN1A"
                     /gene_synonym="CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;
                     SDI1; WAF1"
                     /note="major polyA site"
ORIGIN      
        1 gaggtgtgag cagctgccga agtcagttcc ttgtggagcc ggagctgggc gcggattcgc
       61 cgaggcaccg aggcactcag aggaggcgcc atgtcagaac cggctgggga tgtccgtcag
      121 aacccatgcg gcagcaaggc ctgccgccgc ctcttcggcc cagtggacag cgagcagctg
      181 agccgcgact gtgatgcgct aatggcgggc tgcatccagg aggcccgtga gcgatggaac
      241 ttcgactttg tcaccgagac accactggag ggtgacttcg cctgggagcg tgtgcggggc
      301 cttggcctgc ccaagctcta ccttcccacg gggccccggc gaggccggga tgagttggga
      361 ggaggcaggc ggcctggcac ctcacctgct ctgctgcagg ggacagcaga ggaagaccat
      421 gtggacctgt cactgtcttg tacccttgtg cctcgctcag gggagcaggc tgaagggtcc
      481 ccaggtggac ctggagactc tcagggtcga aaacggcggc agaccagcat gacagatttc
      541 taccactcca aacgccggct gatcttctcc aagaggaagc cctaatccgc ccacaggaag
      601 cctgcagtcc tggaagcgcg agggcctcaa aggcccgctc tacatcttct gccttagtct
      661 cagtttgtgt gtcttaatta ttatttgtgt tttaatttaa acacctcctc atgtacatac
      721 cctggccgcc ccctgccccc cagcctctgg cattagaatt atttaaacaa aaactaggcg
      781 gttgaatgag aggttcctaa gagtgctggg catttttatt ttatgaaata ctatttaaag
      841 cctcctcatc ccgtgttctc cttttcctct ctcccggagg ttgggtgggc cggcttcatg
      901 ccagctactt cctcctcccc acttgtccgc tgggtggtac cctctggagg ggtgtggctc
      961 cttcccatcg ctgtcacagg cggttatgaa attcaccccc tttcctggac actcagacct
     1021 gaattctttt tcatttgaga agtaaacaga tggcactttg aaggggcctc accgagtggg
     1081 ggcatcatca aaaactttgg agtcccctca cctcctctaa ggttgggcag ggtgaccctg
     1141 aagtgagcac agcctagggc tgagctgggg acctggtacc ctcctggctc ttgatacccc
     1201 cctctgtctt gtgaaggcag ggggaaggtg gggtcctgga gcagaccacc ccgcctgccc
     1261 tcatggcccc tctgacctgc actggggagc ccgtctcagt gttgagcctt ttccctcttt
     1321 ggctcccctg taccttttga ggagccccag ctaccctttt tctccagctg ggctctgcaa
     1381 ttcccctctg ctgctgtccc tcccccttgt cctttccctt cagtaccctc tcagctccag
     1441 gtggctctga ggtgcctgtc ccacccccac ccccagctca atggactgga aggggaaggg
     1501 acacacaaga agaagggcac cctagttcta cctcaggcag ctcaagcagc gaccgccccc
     1561 tcctctagct gtgggggtga gggtcccatg tggtggcaca ggcccccttg agtggggtta
     1621 tctctgtgtt aggggtatat gatgggggag tagatctttc taggagggag acactggccc
     1681 ctcaaatcgt ccagcgacct tcctcatcca ccccatccct ccccagttca ttgcactttg
     1741 attagcagcg gaacaaggag tcagacattt taagatggtg gcagtagagg ctatggacag
     1801 ggcatgccac gtgggctcat atggggctgg gagtagttgt ctttcctggc actaacgttg
     1861 agcccctgga ggcactgaag tgcttagtgt acttggagta ttggggtctg accccaaaca
     1921 ccttccagct cctgtaacat actggcctgg actgttttct ctcggctccc catgtgtcct
     1981 ggttcccgtt tctccaccta gactgtaaac ctctcgaggg cagggaccac accctgtact
     2041 gttctgtgtc tttcacagct cctcccacaa tgctgaatat acagcaggtg ctcaataaat
     2101 gattcttagt gacttta
//
