LOCUS       NM_001260               3065 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens cyclin dependent kinase 8 (CDK8), transcript variant
            1, mRNA.
ACCESSION   NM_001260
VERSION     NM_001260.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Comeau,D., Belliveau,J., Bouhamdani,N. and Amor,M.B.
  TITLE     Expanding the phenotypic spectrum for CDK8-related disease: A case
            report
  JOURNAL   Am J Med Genet A 194 (5), e63537 (2024)
   PUBMED   38193604
  REMARK    GeneRIF: Expanding the phenotypic spectrum for CDK8-related
            disease: A case report.
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Choi,H.Y., Zhu,Y., Zhao,X., Mehta,S., Hernandez,J.C., Lee,J.J.,
            Kou,Y., Machida,R., Giacca,M., Del Sal,G., Ray,R., Eoh,H.,
            Tahara,S.M., Chen,L., Tsukamoto,H. and Machida,K.
  TITLE     NOTCH localizes to mitochondria through the TBC1D15-FIS1
            interaction and is stabilized via blockade of E3 ligase and CDK8
            recruitment to reprogram tumor-initiating cells
  JOURNAL   Exp Mol Med 56 (2), 461-477 (2024)
   PUBMED   38409448
  REMARK    GeneRIF: NOTCH localizes to mitochondria through the TBC1D15-FIS1
            interaction and is stabilized via blockade of E3 ligase and CDK8
            recruitment to reprogram tumor-initiating cells.
            Erratum:[Exp Mol Med. 2024 Oct;56(10):2323. doi:
            10.1038/s12276-024-01325-9.. PMID: 39465353]
REFERENCE   3  (bases 1 to 3065)
  AUTHORS   Chen,M., Li,J., Zhang,L., Wang,L., Cheng,C., Ji,H., Altilia,S.,
            Ding,X., Cai,G., Altomare,D., Shtutman,M., Byrum,S.D.,
            Mackintosh,S.G., Feoktistov,A., Soshnikova,N., Mogila,V.A.,
            Tatarskiy,V., Erokhin,M., Chetverina,D., Prawira,A., Ni,Y.,
            Urban,S., McInnes,C., Broude,E.V. and Roninson,I.B.
  TITLE     CDK8 and CDK19: positive regulators of signal-induced transcription
            and negative regulators of Mediator complex proteins
  JOURNAL   Nucleic Acids Res 51 (14), 7288-7313 (2023)
   PUBMED   37378433
  REMARK    GeneRIF: CDK8 and CDK19: positive regulators of signal-induced
            transcription and negative regulators of Mediator complex proteins.
REFERENCE   4  (bases 1 to 3065)
  AUTHORS   Li,J., Bai,Y., Liu,Y., Song,Z., Yang,Y. and Zhao,Y.
  TITLE     Transcriptome-based chemical screens identify CDK8 as a common
            barrier in multiple cell reprogramming systems
  JOURNAL   Cell Rep 42 (6), 112566 (2023)
   PUBMED   37235474
  REMARK    GeneRIF: Transcriptome-based chemical screens identify CDK8 as a
            common barrier in multiple cell reprogramming systems.
REFERENCE   5  (bases 1 to 3065)
  AUTHORS   Rzymski,T., Mikula,M., Wiklik,K. and Brzozka,K.
  TITLE     CDK8 kinase--An emerging target in targeted cancer therapy
  JOURNAL   Biochim Biophys Acta 1854 (10 Pt B), 1617-1629 (2015)
   PUBMED   26006748
  REMARK    GeneRIF: Current state of the art confirms that further development
            of CDK8 inhibitors will translate into targeted therapies in
            oncology
            Review article
REFERENCE   6  (bases 1 to 3065)
  AUTHORS   Nemet,J., Jelicic,B., Rubelj,I. and Sopta,M.
  TITLE     The two faces of Cdk8, a positive/negative regulator of
            transcription
  JOURNAL   Biochimie 97, 22-27 (2014)
   PUBMED   24139904
  REMARK    GeneRIF: CDK8 regulates the transcription factors turnover,
            C-terminal domain phosphorylation, and regulates the activator or
            repressor functions. (Review)
            Review article
REFERENCE   7  (bases 1 to 3065)
  AUTHORS   Cujec,T.P., Cho,H., Maldonado,E., Meyer,J., Reinberg,D. and
            Peterlin,B.M.
  TITLE     The human immunodeficiency virus transactivator Tat interacts with
            the RNA polymerase II holoenzyme
  JOURNAL   Mol Cell Biol 17 (4), 1817-1823 (1997)
   PUBMED   9121429
REFERENCE   8  (bases 1 to 3065)
  AUTHORS   Rickert,P., Seghezzi,W., Shanahan,F., Cho,H. and Lees,E.
  TITLE     Cyclin C/CDK8 is a novel CTD kinase associated with RNA polymerase
            II
  JOURNAL   Oncogene 12 (12), 2631-2640 (1996)
   PUBMED   8700522
REFERENCE   9  (bases 1 to 3065)
  AUTHORS   Tassan,J.P., Jaquenoud,M., Leopold,P., Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of human cyclin-dependent kinase 8, a putative
            protein kinase partner for cyclin C
  JOURNAL   Proc Natl Acad Sci U S A 92 (19), 8871-8875 (1995)
   PUBMED   7568034
REFERENCE   10 (bases 1 to 3065)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC094784.1, BC104492.1 and
            BM021447.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001260.2.
            
            Summary: This gene encodes a member of the cyclin-dependent protein
            kinase (CDK) family. CDK family members are known to be important
            regulators of cell cycle progression. This kinase and its
            regulatory subunit, cyclin C, are components of the Mediator
            transcriptional regulatory complex, involved in both
            transcriptional activation and repression by phosphorylation of the
            carboxy-terminal domain of the largest subunit of RNA polymerase
            II. This kinase regulates transcription by targeting the
            cyclin-dependent kinase 7 subunits of the general transcription
            initiation factor IIH, thus providing a link between the Mediator
            complex and the basal transcription machinery. Multiple pseudogenes
            of this gene have been identified. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1113401.1,
                                           SRR3476690.615108.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381527.8/ ENSP00000370938.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-490               BC094784.1         26-515
            491-2007            BC104492.1         4-1520
            2008-3055           BC094784.1         1975-3022
            3056-3065           BM021447.1         380-389
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.13"
     gene            1..3065
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /note="cyclin dependent kinase 8"
                     /db_xref="GeneID:1024"
                     /db_xref="HGNC:HGNC:1779"
                     /db_xref="MIM:603184"
     exon            1..641
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     CDS             514..1908
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /EC_number="2.7.11.23"
                     /EC_number="2.7.11.22"
                     /note="isoform 1 is encoded by transcript variant 1; CDK8
                     protein kinase; protein kinase K35; cell division protein
                     kinase 8; mediator complex subunit CDK8; mediator of RNA
                     polymerase II transcription subunit CDK8"
                     /codon_start=1
                     /product="cyclin-dependent kinase 8 isoform 1"
                     /protein_id="NP_001251.1"
                     /db_xref="CCDS:CCDS9317.1"
                     /db_xref="GeneID:1024"
                     /db_xref="HGNC:HGNC:1779"
                     /db_xref="MIM:603184"
                     /translation="MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGK
                     DDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEH
                     DLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVM
                     GEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIW
                     AIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEH
                     STLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPY
                     FLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQD
                     SSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSAT
                     SQQPPQYSHQTHRY"
     exon            642..717
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            718..828
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            829..969
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1027
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1159
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1303
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1373
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1446
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1544
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1623
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1782
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..3065
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3039..3044
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /note="hexamer: AATAAA"
     polyA_site      3065
                     /gene="CDK8"
                     /gene_synonym="IDDHBA; K35"
                     /note="major polyA site"
ORIGIN      
        1 gaggaggtac cggctgttgt gcggctctgc ccttctgttt gagtgtatgg gagagtgagt
       61 gagtgagtga gtgtgagcgt gtgtgtgaga gcgtgaggcg tgagtgcgcg tgtgagagga
      121 cgagagcccg cctggccgcc ccgccgctcc cgccgcagca ggagcagaac gcgcggccgg
      181 agagagcggc ggagccggcg cccagggagc ccgcggggac aagggcagag acaccgctcc
      241 ccacccccag ccctcgtccc tcggctctcc ttcgccgggg gatcctcccc gttcctccac
      301 ccccggccgg cctctgcccc gccgtccccc tggatgtccc tggcgctttc gcggggcctc
      361 ctcctgctct tgccgcatca gtcgggctgg tgctgcggcc ggcgggcgta gagcgggcgg
      421 gttcccgggg gctgcggctg cccgtgcttc cccggtcccc acccctgccc cccggccccc
      481 cgacccagct ctccggcctc agaggctgtg acaatggact atgactttaa agtgaagctg
      541 agcagcgagc gggagcgggt cgaggacctg tttgaatacg agggctgcaa agttggccga
      601 ggcacttatg gtcacgtcta caaagccaag aggaaagatg ggaaggatga taaagactat
      661 gctttaaaac aaatagaagg aactgggatc tctatgtcgg catgtagaga aatagcatta
      721 cttcgagagc ttaagcatcc aaacgtcatt tctcttcaaa aggtgtttct gtctcatgct
      781 gataggaagg tgtggcttct gtttgactat gctgaacatg acctctggca tataatcaag
      841 tttcacagag cttctaaagc aaacaagaag ccagttcagt tacctcgggg aatggtgaag
      901 tcactattat atcagatcct agatggtatt cactacctgc atgctaactg ggtgttgcac
      961 agagatttga aacctgctaa tattttagtt atgggtgaag gtcctgagcg aggaagagta
     1021 aaaattgctg acatgggctt tgcccgatta tttaattcac ctttgaagcc tttagcagat
     1081 ttggatccag tggttgttac attctggtac cgagcccctg aactacttct tggagcaagg
     1141 cattatacca aagctattga tatttgggct atagggtgta tatttgcaga actactaacg
     1201 tcagaaccaa tatttcactg tcgacaagag gacatcaaaa ctagtaatcc ttatcaccat
     1261 gaccagctgg acagaatatt caatgtaatg ggatttcctg cagataaaga ttgggaagat
     1321 ataaaaaaga tgcctgaaca ttcaacatta atgaaagatt tcagaagaaa tacgtatacc
     1381 aactgcagcc ttatcaagta tatggaaaaa cataaagtta aaccagatag taaagcattc
     1441 cacttgcttc agaagctgct taccatggac ccaataaagc gaattacctc agaacaggct
     1501 atgcaggacc cctatttctt agaagaccca cttcctacat cagacgtttt tgccggttgt
     1561 caaatccctt acccaaaacg agaattttta acggaagaag aacctgatga caaaggagac
     1621 aaaaagaacc agcagcagca gcagggcaat aaccacacta atggaactgg ccacccaggg
     1681 aatcaagaca gcagtcacac acagggaccc ccgttgaaga aagtgagagt tgttcctcct
     1741 accactacct caggtggact tatcatgacc tcagactatc agcgttccaa tccacatgct
     1801 gcctatccca accctggacc aagcacatca cagccgcaga gcagcatggg atactcagct
     1861 acctcccagc agcctccaca gtactcacat cagacacatc ggtactgagc tgcatcggaa
     1921 tcttgtccat gcactgttgc gaatgctgca gggctgactg tgcagctctc tgcgggaacc
     1981 tggtatgggc catgagaatg tactgtacaa ccacatcttc aaaatgtcca gtagccaagt
     2041 tccaccactt ttcacagatt ggggtagtgg cttccaagtt gtacctattt tggagttaga
     2101 cttgaaaaga aagtgctagc acagtttgtg ttgtggattt gctacttcca tagtttactt
     2161 gacatggttc agactgacca atgcattttt ttcagtgaca gtctgtagca gttgaagctg
     2221 tgaatgtgct aggggcaagc atttgtcttt gtatgtggtg aattttttca gtgtaacaac
     2281 attatctgac caatagtaca cacacagaca caaagtttaa ctggtacttg aaacatacag
     2341 tatatgttaa cgaaataacc aagactcgaa atgagattat tttggtacac ctttcttttt
     2401 agtgtcttat cagtgggctg attcattttc tacattaatc agtgttttct gaccaagaat
     2461 attgcttgga tttttttgaa agtacaaaaa gccacatagt ttttccagaa aggtttcaaa
     2521 actcccaaag attaacttcc aacttataag tttgttttta ttttcaatct atgacttgac
     2581 tggtattaaa gctgctattt gatagtaatt aaatatgttg tcattgatat aaacctgttt
     2641 ggttcagcaa acaaactaaa atgattgtca tagacagtgt tttatttttc ctgttggtgt
     2701 tgctgatttg tgagcatgct ttaagatgaa aaaagcatga atgataactt ccttaaaaag
     2761 gtgcggcatc caattcaaat attttcgtcc tgattttaaa gctggttggt gtagtgctat
     2821 taaaatttcg ttcagttaat tttccttttg aaaacttgtt cgcacgttgt ttagggtgcc
     2881 cttacttcag caaaggagaa ggagtaggag agccttagaa tttttgagga aaaaaaaacc
     2941 tataacatac aatgtactgt atcaaactat tttacatgaa tgacacaagt attctgaata
     3001 aaaaataatt gaacattgtt aaaaacaagg tgttatgtaa taaatttatt tttcataaat
     3061 caaaa
//
