LOCUS       NM_001258               1620 bp    mRNA    linear   PRI 01-MAY-2025
DEFINITION  Homo sapiens cyclin dependent kinase 3 (CDK3), mRNA.
ACCESSION   NM_001258
VERSION     NM_001258.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1620)
  AUTHORS   Liu,Z.H., Yang,S.Z., Li,W.Y., Dong,S.Y., Zhou,S.Y. and Xu,S.
  TITLE     circRNA_141539 can serve as an oncogenic factor in esophageal
            squamous cell carcinoma by sponging miR-4469 and activating CDK3
            gene
  JOURNAL   Aging (Albany NY) 13 (8), 12179-12193 (2021)
   PUBMED   33504681
  REMARK    GeneRIF: circRNA_141539 can serve as an oncogenic factor in
            esophageal squamous cell carcinoma by sponging miR-4469 and
            activating CDK3 gene.
REFERENCE   4  (bases 1 to 1620)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1620)
  AUTHORS   Zhang,Y., Yang,L., Ling,C. and Heng,W.
  TITLE     HuR facilitates cancer stemness of lung cancer cells via regulating
            miR-873/CDK3 and miR-125a-3p/CDK3 axis
  JOURNAL   Biotechnol Lett 40 (4), 623-631 (2018)
   PUBMED   29344850
  REMARK    GeneRIF: HuR facilitated lung cancer stemness dependent on CDK3
            expression. miR-873 or miR-125a-3p level was negatively correlated
            with HuR and CDK3 expression levels in lung cancer tissues.  HuR
            facilitates lung cancer stemness via regulating miR-873/CDK3 and
            miR-125a-3p/CDK3 axis.
REFERENCE   6  (bases 1 to 1620)
  AUTHORS   Meikrantz,W. and Schlegel,R.
  TITLE     Suppression of apoptosis by dominant negative mutants of
            cyclin-dependent protein kinases
  JOURNAL   J Biol Chem 271 (17), 10205-10209 (1996)
   PUBMED   8626584
REFERENCE   7  (bases 1 to 1620)
  AUTHORS   Sasaguri,T., Ishida,A., Kosaka,C., Nojima,H. and Ogata,J.
  TITLE     Phorbol ester inhibits the phosphorylation of the retinoblastoma
            protein without suppressing cyclin D-associated kinase in vascular
            smooth muscle cells
  JOURNAL   J Biol Chem 271 (14), 8345-8351 (1996)
   PUBMED   8626531
REFERENCE   8  (bases 1 to 1620)
  AUTHORS   Bullrich,F., MacLachlan,T.K., Sang,N., Druck,T., Veronese,M.L.,
            Allen,S.L., Chiorazzi,N., Koff,A., Heubner,K., Croce,C.M. et al.
  TITLE     Chromosomal mapping of members of the cdc2 family of protein
            kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor,
            p27Kip1, to regions involved in human cancer
  JOURNAL   Cancer Res 55 (6), 1199-1205 (1995)
   PUBMED   7882308
REFERENCE   9  (bases 1 to 1620)
  AUTHORS   Gyuris,J., Golemis,E., Chertkov,H. and Brent,R.
  TITLE     Cdi1, a human G1 and S phase protein phosphatase that associates
            with Cdk2
  JOURNAL   Cell 75 (4), 791-803 (1993)
   PUBMED   8242750
REFERENCE   10 (bases 1 to 1620)
  AUTHORS   Meyerson,M., Enders,G.H., Wu,C.L., Su,L.K., Gorka,C., Nelson,C.,
            Harlow,E. and Tsai,L.H.
  TITLE     A family of human cdc2-related protein kinases
  JOURNAL   EMBO J 11 (8), 2909-2917 (1992)
   PUBMED   1639063
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC040980.14.
            
            On Apr 29, 2021 this sequence version replaced NM_001258.3.
            
            Summary: This gene encodes a member of the cyclin-dependent protein
            kinase family. The protein promotes entry into S phase, in part by
            activating members of the E2F family of transcription factors. The
            protein also associates with cyclin C and phosphorylates the
            retinoblastoma 1 protein to promote exit from G0. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: A downstream translational start codon is selected
            for this RefSeq based on its better conservation in mammalian
            species and on a strong Kozak signal. An upstream in-frame start
            codon is also present but has a weaker Kozak signal and is poorly
            conserved. Use of the upstream start codon would result in a
            protein that is 20 aa longer at the N-terminus. Leaky scanning by
            ribosomes may allow translation initiation at the downstream start
            codon.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.814957.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000448471.3/
                                                ENSP00000400088.1
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               AC040980.14        72549-72661         c
            114-243             AC040980.14        71975-72104         c
            244-321             AC040980.14        71565-71642         c
            322-442             AC040980.14        71374-71494         c
            443-613             AC040980.14        71098-71268         c
            614-715             AC040980.14        70904-71005         c
            716-919             AC040980.14        70118-70321         c
            920-1620            AC040980.14        67518-68218         c
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..1620
                     /gene="CDK3"
                     /note="cyclin dependent kinase 3"
                     /db_xref="GeneID:1018"
                     /db_xref="HGNC:HGNC:1772"
                     /db_xref="MIM:123828"
     exon            1..113
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     exon            114..243
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..1045
                     /gene="CDK3"
                     /EC_number="2.7.11.22"
                     /note="cell division protein kinase 3"
                     /codon_start=1
                     /product="cyclin-dependent kinase 3"
                     /protein_id="NP_001249.1"
                     /db_xref="CCDS:CCDS11736.1"
                     /db_xref="GeneID:1018"
                     /db_xref="HGNC:HGNC:1772"
                     /db_xref="MIM:123828"
                     /translation="MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGV
                     PSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLH
                     LIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYT
                     HEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRM
                     LGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRIT
                     AKTALAHPYFSSPEPSPAARQYVLQRFRH"
     exon            244..321
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     exon            322..442
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     exon            443..613
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     exon            614..715
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     exon            716..919
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1620
                     /gene="CDK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1599..1604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK3"
                     /note="hexamer: AATAAA"
     polyA_site      1620
                     /gene="CDK3"
                     /note="major polyA site"
ORIGIN      
        1 agaccctcct gacccctgac ctctgccccc agtggccctg gcccctgggc agcccttcct
       61 ggccgccatg tgtacccaga gcctgggact ggctgggctg ggcagtgacc caggcagctc
      121 tgtggccatg gatatgttcc agaaggtaga gaagatcgga gagggcacct atggggtggt
      181 gtacaaggcc aagaacaggg agacagggca gctggtggcc ctgaagaaga tcagactgga
      241 tttggagatg gagggggtcc caagcactgc catcagggag atctcgctgc tcaaggaact
      301 gaagcacccc aacatcgtcc gactgctgga cgtggtgcac aacgagagga agctctatct
      361 ggtgtttgag ttcctcagcc aggacctgaa gaagtacatg gactccaccc caggctcaga
      421 gctccccctg cacctcatca agagctacct cttccagctg ctgcaggggg tgagtttctg
      481 ccactcacat cgggtcatcc accgagacct gaagccccag aacctgctca tcaatgagtt
      541 gggtgccatc aagctggctg acttcggcct ggctcgcgcc ttcggggtgc ccctgcgcac
      601 ctacacccat gaggtggtga cactgtggta tcgcgccccc gagattctct tgggcagcaa
      661 gttctatacc acagctgtgg atatctggag cattggttgc atctttgcag agatggtgac
      721 tcgaaaagcc ctgtttcctg gtgactctga gattgaccag ctctttcgta tctttcgtat
      781 gctggggaca cccagcgaag acacatggcc cggggtcacc cagctgcctg actataaggg
      841 cagcttccct aagtggacca ggaagggact ggaagagatt gtgcccaatc tggagccaga
      901 gggcagggac ctgctcatgc aactcctgca gtatgacccc agccagcgga tcacagccaa
      961 gactgccctg gcccacccgt acttctcatc ccctgagccc tccccagctg cccgccagta
     1021 tgtgctgcag cgattccgcc attgagaatg tcaaggccac actcagatcc tttctcgagc
     1081 agctgctgcc ccagctgcct cctacccatt gccaagagag gatgcatctg gggagagcaa
     1141 agcactaagg aattcagcat cagcctgcag agggctgagt ctgggttagt cctgcccgca
     1201 ggttagcgag atcctgtgtt gttttttggg ttggacggtg ccgtttcaaa atagaggcac
     1261 agatgctcca tgcacgaggg gtccccgggc actggaacaa gtgccaagtt gaaggcaggg
     1321 ggcctgccag agctgggtgt gggtattcag gccctcacct gccctgcctg cagggccaga
     1381 ccctgaggaa agggcgcccc ctgctggtct ttttggattt aaaatgtttg ggggaagagt
     1441 tgagttccag ttcagtccca agttaaacag gagggagtga gggagaggag agagggcgtt
     1501 ccccggatcc caggagagct gggctatgac tgcaataagg agttgttcct tcacctgaga
     1561 tgtgcttctt ttggttcatt tctggcttga caacaataaa taaacgtggt atgttcctga
//
