LOCUS       NM_001039803            2149 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens cyclin dependent kinase 20 (CDK20), transcript variant
            3, mRNA.
ACCESSION   NM_001039803
VERSION     NM_001039803.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Chivukula,S. and Malkhed,V.
  TITLE     The Role of CDK20 Protein in Carcinogenesis
  JOURNAL   Curr Drug Targets 24 (10), 790-796 (2023)
   PUBMED   37469151
  REMARK    GeneRIF: The Role of CDK20 Protein in Carcinogenesis.
            Review article
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Satoda,Y., Noguchi,T., Fujii,T., Taniguchi,A., Katoh,Y. and
            Nakayama,K.
  TITLE     BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36
            contributes to intraflagellar transport turnaround involving
            ICK/CILK1
  JOURNAL   Mol Biol Cell 33 (9), ar79 (2022)
   PUBMED   35609210
  REMARK    GeneRIF: BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36
            contributes to intraflagellar transport turnaround involving
            ICK/CILK1.
REFERENCE   3  (bases 1 to 2149)
  AUTHORS   Zhou,J., Liu,M., Sun,H., Feng,Y., Xu,L., Chan,A.W.H., Tong,J.H.,
            Wong,J., Chong,C.C.N., Lai,P.B.S., Wang,H.K., Tsang,S.W.,
            Goodwin,T., Liu,R., Huang,L., Chen,Z., Sung,J.J., Chow,K.L.,
            To,K.F. and Cheng,A.S.
  TITLE     Hepatoma-intrinsic CCRK inhibition diminishes myeloid-derived
            suppressor cell immunosuppression and enhances immune-checkpoint
            blockade efficacy
  JOURNAL   Gut 67 (5), 931-944 (2018)
   PUBMED   28939663
  REMARK    GeneRIF: Results delineate an immunosuppressive mechanism of the
            hepatoma-intrinsic CCRK signalling and highlight an overexpressed
            kinase target whose inhibition might empower hepatocellular
            carcinoma immunotherapy
REFERENCE   4  (bases 1 to 2149)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   5  (bases 1 to 2149)
  AUTHORS   Feng,H., Yu,Z., Tian,Y., Lee,Y.Y., Li,M.S., Go,M.Y., Cheung,Y.S.,
            Lai,P.B., Chan,A.M., To,K.F., Chan,H.L., Sung,J.J. and Cheng,A.S.
  TITLE     A CCRK-EZH2 epigenetic circuitry drives hepatocarcinogenesis and
            associates with tumor recurrence and poor survival of patients
  JOURNAL   J Hepatol 62 (5), 1100-1111 (2015)
   PUBMED   25500144
  REMARK    GeneRIF: A CCRK-EZH2 epigenetic circuitry drives
            hepatocarcinogenesis and associates with tumor recurrence and poor
            survival of patient.
REFERENCE   6  (bases 1 to 2149)
  AUTHORS   Wu,G.Q., Xie,D., Yang,G.F., Liao,Y.J., Mai,S.J., Deng,H.X., Sze,J.,
            Guan,X.Y., Zeng,Y.X., Lin,M.C. and Kung,H.F.
  TITLE     Cell cycle-related kinase supports ovarian carcinoma cell
            proliferation via regulation of cyclin D1 and is a predictor of
            outcome in patients with ovarian carcinoma
  JOURNAL   Int J Cancer 125 (11), 2631-2642 (2009)
   PUBMED   19672860
  REMARK    GeneRIF: Overexpression of CCRK is associated with ovarian
            carcinoma.
REFERENCE   7  (bases 1 to 2149)
  AUTHORS   Malumbres,M., Harlow,E., Hunt,T., Hunter,T., Lahti,J.M.,
            Manning,G., Morgan,D.O., Tsai,L.H. and Wolgemuth,D.J.
  TITLE     Cyclin-dependent kinases: a family portrait
  JOURNAL   Nat Cell Biol 11 (11), 1275-1276 (2009)
   PUBMED   19884882
REFERENCE   8  (bases 1 to 2149)
  AUTHORS   Qiu,H., Dai,H., Jain,K., Shah,R., Hong,C., Pain,J., Tian,B.,
            Vatner,D.E., Vatner,S.F. and Depre,C.
  TITLE     Characterization of a novel cardiac isoform of the cell
            cycle-related kinase that is regulated during heart failure
  JOURNAL   J Biol Chem 283 (32), 22157-22165 (2008)
   PUBMED   18508765
REFERENCE   9  (bases 1 to 2149)
  AUTHORS   Wohlbold,L., Larochelle,S., Liao,J.C., Livshits,G., Singer,J.,
            Shokat,K.M. and Fisher,R.P.
  TITLE     The cyclin-dependent kinase (CDK) family member PNQALRE/CCRK
            supports cell proliferation but has no intrinsic CDK-activating
            kinase (CAK) activity
  JOURNAL   Cell Cycle 5 (5), 546-554 (2006)
   PUBMED   16552187
  REMARK    GeneRIF: Depletion of PNQALRE by >80% due to RNA interference
            (RNAi) impairs cell proliferation, but fails to arrest the cell
            cycle at a discrete point.
REFERENCE   10 (bases 1 to 2149)
  AUTHORS   Liu,Y., Wu,C. and Galaktionov,K.
  TITLE     p42, a novel cyclin-dependent kinase-activating kinase in mammalian
            cells
  JOURNAL   J Biol Chem 279 (6), 4507-4514 (2004)
   PUBMED   14597612
  REMARK    GeneRIF: identification of p42, a novel cyclin-dependent
            kinase-activating kinase (CAK) activity in human cells; p42 is
            essential for the phosphorylation of Thr-160 and activation of CDK2
            and is indispensable for cell growth
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353572.13, AI479563.1 and
            BM982394.1.
            
            On Feb 27, 2020 this sequence version replaced NM_001039803.2.
            
            Summary: The protein encoded by this gene contains a kinase domain
            most closely related to the cyclin-dependent protein kinases. The
            encoded kinase may activate cyclin-dependent kinase 2 and is
            involved in cell growth. Alternatively spliced transcript variants
            encoding distinct isoforms have been reported. [provided by RefSeq,
            Dec 2009].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 5' coding region and contains an alternate,
            in-frame exon in the middle portion of the coding region, compared
            to variant 1. This results in a longer protein (isoform 3),
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.3123880.1,
                                           SRR18074969.3611155.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158188, SAMN03267762
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325303.9/ ENSP00000322343.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AL353572.13        72375-72536         c
            163-276             AL353572.13        71925-72038         c
            277-465             AL353572.13        69150-69338         c
            466-587             AL353572.13        68779-68900         c
            588-650             AL353572.13        68571-68633         c
            651-774             AL353572.13        67799-67922         c
            775-930             AL353572.13        67197-67352         c
            931-1956            AL353572.13        64637-65662         c
            1957-2144           AI479563.1         1-188               c
            2145-2149           BM982394.1         12-16               c
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.1"
     gene            1..2149
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /note="cyclin dependent kinase 20"
                     /db_xref="GeneID:23552"
                     /db_xref="HGNC:HGNC:21420"
                     /db_xref="MIM:610076"
     exon            1..162
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1128
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /EC_number="2.7.11.22"
                     /note="isoform 3 is encoded by transcript variant 3; cell
                     division protein kinase 20; CAK-kinase p42; CDK-activating
                     kinase p42; cyclin-kinase-activating kinase p42;
                     cyclin-dependent protein kinase H; cell cycle-related
                     kinase"
                     /codon_start=1
                     /product="cyclin-dependent kinase 20 isoform 3"
                     /protein_id="NP_001034892.1"
                     /db_xref="CCDS:CCDS35060.1"
                     /db_xref="GeneID:23552"
                     /db_xref="HGNC:HGNC:21420"
                     /db_xref="MIM:610076"
                     /translation="MDQYCILGRIGEGAHGIVFKAKHVETGEIVALKKVALRRLEDGF
                     PNQALREIKALQEMEDNQYVVQLKAVFPHGGGFVLAFEFMLSDLAEVVRHAQRPLAQA
                     QVKSYLQMLLKGVAFCHANNIVHRDLKPANLLISASGQLKIADFGLARVFSPDGSRLY
                     THQVATRWYRAPELLYGARQYDQGVDLWSVGCIMGELLNGSPLFPGKNDIEQLCYVLR
                     ILGTPNPQVWPELTELPDYNKISFKEQVPMPLEEVLPDVSPQALDLLGQFLLYPPHQR
                     IAASKALLHQYFFTAPLPAHPSELPIPQRLGGPAPKAHPGPPHIHDFHVDRPLEESLL
                     NPELIRPFILEG"
     exon            163..276
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     exon            277..465
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     exon            466..587
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     exon            588..650
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     exon            651..774
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     exon            775..930
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     exon            931..2149
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1958..1963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /note="hexamer: AATAAA"
     polyA_site      1979
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /note="major polyA site"
     regulatory      2116..2121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /note="hexamer: AATAAA"
     polyA_site      2149
                     /gene="CDK20"
                     /gene_synonym="CCRK; CDCH; P42; PNQALRE"
                     /note="major polyA site"
ORIGIN      
        1 gtgcgagctc agatcagcgt ggggtggagg agaagtggag tttggaagtt caggggcaca
       61 ggggcacagg cccacgactg cagcgggatg gaccagtact gcatcctggg ccgcatcggg
      121 gagggcgccc acggcatcgt cttcaaggcc aagcacgtgg agactggcga gatagttgcc
      181 ctcaagaagg tggccctaag gcggttggag gacggcttcc ctaaccaggc cctgcgggag
      241 attaaggctc tgcaggagat ggaggacaat cagtatgtgg tacaactgaa ggctgtgttc
      301 ccacacggtg gaggctttgt gctggccttt gagttcatgc tgtcggatct ggccgaggtg
      361 gtgcgccatg cccagaggcc actagcccag gcacaggtca agagctacct gcagatgctg
      421 ctcaagggtg tcgccttctg ccatgccaac aacattgtac atcgggacct gaaacctgcc
      481 aacctgctca tcagcgcctc aggccagctc aagatagcgg actttggcct ggctcgagtc
      541 ttttccccag acggcagccg cctctacaca caccaggtgg ccaccaggtg gtaccgagcc
      601 cccgagctcc tgtatggtgc ccgccagtat gaccagggcg tcgatctgtg gtctgtgggc
      661 tgcatcatgg gggagctgtt gaatgggtcc ccccttttcc cgggcaagaa cgatattgaa
      721 cagctttgct atgtgcttcg catcttgggc accccaaacc ctcaagtctg gccggagctc
      781 actgagctgc cggactacaa caagatctcc tttaaggagc aggtgcccat gcccctggag
      841 gaggtgctgc ctgacgtctc tccccaggca ttggatctgc tgggtcaatt ccttctctac
      901 cctcctcacc agcgcatcgc agcttccaag gctctcctcc atcagtactt cttcacagct
      961 cccctgcctg cccatccatc tgagctgccg attcctcagc gtctaggggg acctgccccc
     1021 aaggcccatc cagggccccc ccacatccat gacttccacg tggaccggcc tcttgaggag
     1081 tcgctgttga acccagagct gattcggccc ttcatcctgg aggggtgaga agttggccct
     1141 ggtcccgtct gcctgctcct caggaccact cagtccacct gttcctctgc cacctgcctg
     1201 gcttcaccct ccaaggcctc cccatggcca cagtgggccc acaccacacc ctgcccctta
     1261 gcccttgcga gggttggtct cgaggcagag gtcatgttcc cagccaagag tatgagaaca
     1321 tccagtcgag cagaggagat tcatggcctg tgctcggtga gccttacctt ctgtgtgcta
     1381 ctgacgtacc catcaggaca gtgagctctg ctgccagtca aggcctgcat atgcagaatg
     1441 acgatgcctg ccttggtgct gcttccccga gtgctgcctc ctggtcaagg agaagtgcag
     1501 agagtaaggt gtccttatgt tggaaactca agtggaagga agatttggtt tggttttatt
     1561 ctcagagcca ttaaacacta gttcagtatg tgagatatag attctaaaaa cctcaggtgg
     1621 ctctgcctta tgtctgttcc tccttcattt ctctcaaggg aaatggctaa ggtggcattg
     1681 tctcatggct ctcgtttttg gggtcatggg gagggtagca ccaggcatag ccacttttgc
     1741 cctgagggac tcctgtgtac ttcacatcac tgagcactca tttagaagtg agggagacag
     1801 aagtctaggc ccagggatgg ctccagttgg ggatccagca ggagaccctc tgcacatgag
     1861 gctggtttac caacatctac tccctcagga tgagcgtgag ccagaagcag ctgtgtattt
     1921 aaggaaacaa gcgttcctgg aattaattta taaatttaat aaatcccaat ataatcccag
     1981 ctagtgcttt ttccttatta taatttgata aggtgattat aaaagataca tggaaggaag
     2041 tggaaccaga tgcagaagag gaaatgatgg aaggacttac ggtatcagat accaatattt
     2101 aaaagtttgt ataataataa agagtatgat tgtggttcaa ggataaaaa
//
