LOCUS       NM_212502               2986 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens cyclin dependent kinase 18 (CDK18), transcript variant
            2, mRNA.
ACCESSION   NM_212502
VERSION     NM_212502.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Earley,E.J., Kelly,S., Fang,F., Alencar,C.S., Rodrigues,D.O.W.,
            Soares Cruz,D.T., Flanagan,J.M., Ware,R.E., Zhang,X., Gordeuk,V.,
            Gladwin,M., Zhang,Y., Nouraie,M., Nekhai,S., Sabino,E., Custer,B.,
            Dinardo,C. and Page,G.P.
  CONSRTM   International Component of the NHLBI Recipient Epidemiology and
            Donor Evaluation Study (REDS-III) and the NHLBI Trans-Omics for
            Precision Medicine (TOPMed) Consortium
  TITLE     Genome-wide association study of early ischaemic stroke risk in
            Brazilian individuals with sickle cell disease implicates ADAMTS2
            and CDK18 and uncovers novel loci
  JOURNAL   Br J Haematol 201 (2), 343-352 (2023)
   PUBMED   36602125
  REMARK    GeneRIF: Genome-wide association study of early ischaemic stroke
            risk in Brazilian individuals with sickle cell disease implicates
            ADAMTS2 and CDK18 and uncovers novel loci.
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 2986)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2986)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 2986)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2986)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   7  (bases 1 to 2986)
  AUTHORS   Herskovits,A.Z. and Davies,P.
  TITLE     Cloning and expression analysis of two novel PCTAIRE 3 transcripts
            from human brain
  JOURNAL   Gene 328, 59-67 (2004)
   PUBMED   15019984
  REMARK    GeneRIF: Expression of PCTAIRE 3a (474 amino acids) was evident
            throughout the brain and in the majority of tissues analyzed, while
            spliced isoform PCTAIRE 3b (504 amino acids) was limited to several
            subcortical nuclei of the basal gangli and the spinal cord.
REFERENCE   8  (bases 1 to 2986)
  AUTHORS   Okuda,T., Valentine,V.A., Shapiro,D.N. and Downing,J.R.
  TITLE     Cloning of genomic loci and chromosomal localization of the human
            PCTAIRE-1 and -3 protein kinase genes
  JOURNAL   Genomics 21 (1), 217-221 (1994)
   PUBMED   8088790
REFERENCE   9  (bases 1 to 2986)
  AUTHORS   Okuda,T., Cleveland,J.L. and Downing,J.R.
  TITLE     PCTAIRE-1 and PCTAIRE-3, two members of a novel cdc2/CDC28-related
            protein kinase gene family
  JOURNAL   Oncogene 7 (11), 2249-2258 (1992)
   PUBMED   1437147
REFERENCE   10 (bases 1 to 2986)
  AUTHORS   Meyerson,M., Enders,G.H., Wu,C.L., Su,L.K., Gorka,C., Nelson,C.,
            Harlow,E. and Tsai,L.H.
  TITLE     A family of human cdc2-related protein kinases
  JOURNAL   EMBO J 11 (8), 2909-2917 (1992)
   PUBMED   1639063
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY353237.1, AB209307.1 and BM695474.1.
            
            On Jun 2, 2019 this sequence version replaced NM_212502.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in a coding exon compared to variant 1. The resulting
            isoform (b) has the same N- and C-termini but is shorter compared
            to isoform a. Variants 2 and 3 both encode isoform b.
            
            CCDS Note: This CCDS representation uses the 5'-most in-frame start
            codon found in the transcript. It should be noted that this start
            codon has a weak Kozak signal, and its conservation is restricted
            to primate species, squirrel, rock hyrax and armadillo. Two better
            conserved potential start codons with stronger Kozak signals are
            located two and five codons downstream, respectively. It is
            possible that leaky scanning by ribosomes would allow one of the
            downstream start codons to be used some of the time. There is no
            experimental evidence indicating which start codon is
            preferentially used in vivo.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.189512.1,
                                           SRR18074969.1538134.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000429964.7/ ENSP00000399082.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1574              AY353237.1         29-1602
            1575-2981           AB209307.1         2350-3756
            2982-2986           BM695474.1         484-488
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..2986
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /note="cyclin dependent kinase 18"
                     /db_xref="GeneID:5129"
                     /db_xref="HGNC:HGNC:8751"
                     /db_xref="MIM:169190"
     exon            1..128
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            129..279
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1574
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /EC_number="2.7.11.22"
                     /note="isoform b is encoded by transcript variant 2;
                     PCTAIRE protein kinase 3; cell division protein kinase 18;
                     PCTAIRE-motif protein kinase 3; serine/threonine-protein
                     kinase PCTAIRE-3"
                     /codon_start=1
                     /product="cyclin-dependent kinase 18 isoform b"
                     /protein_id="NP_997667.1"
                     /db_xref="CCDS:CCDS44300.1"
                     /db_xref="GeneID:5129"
                     /db_xref="HGNC:HGNC:8751"
                     /db_xref="MIM:169190"
                     /translation="MIMNKMKNFKRRFSLSVPRTETIEESLAEFTEQFNQLHNRRNEN
                     LQLGPLGRDPPQECSTFSPTDSGEEPGQLSPGVQFQRRQNQRRFSMEDVSKRLSLPMD
                     IRLPQEFLQKLQMESPDLPKPLSRMSRRASLSDIGFGKLETYVKLDKLGEGTYATVFK
                     GRSKLTENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANIVTLHDLIHTDRSLTLV
                     FEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRGLAYCHHRKILHRDLKPQNLLINER
                     GELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYSTPIDMWGVGCIHYEM
                     ATGRPLFPGSTVKEELHLIFRLLGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINHAP
                     RLDTDGIHLLSSLLLYESKSRMSAEAALSHSYFRSLGERVHQLEDTASIFSLKEIQLQ
                     KDPGYRGLAFQQPGRGKNRRQSIF"
     exon            280..422
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            423..548
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            549..605
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            606..720
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            721..815
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            816..878
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1002
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1123
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1221
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1327
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1370
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1461
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1539
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..2986
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2962..2967
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /note="hexamer: AATATA"
     polyA_site      2986
                     /gene="CDK18"
                     /gene_synonym="PCTAIRE; PCTAIRE3; PCTK3"
                     /note="major polyA site"
ORIGIN      
        1 ccttctacct cctcctcggc cagctcaggt tgcagcttct ctggggaact gctcaccttt
       61 ccggagcagg ggaagctgcc ccgtgcccgg gagggagcgg gcgcaccgcg gcccccagga
      121 cacgcgctga cccggctgcc cagtccctca tgatcatgaa caagatgaag aactttaagc
      181 gccgtttctc cctgtcagtg ccccgcactg agaccattga agaatccttg gctgaattca
      241 cggagcaatt caaccagctc cacaaccggc ggaatgagaa cttgcagctc ggtcctcttg
      301 gcagagaccc cccgcaggag tgcagcacct tctccccaac agacagcggg gaggagccgg
      361 ggcagctctc ccctggcgtg cagttccagc ggcggcagaa ccagcgccgc ttctccatgg
      421 aggacgtcag caagaggctc tctctgccca tggatatccg cctgccccag gaattcctac
      481 agaagctaca gatggagagc ccagatctgc ccaagccgct cagccgcatg tcccgccggg
      541 cctccctgtc agacattggc tttgggaaac tggaaacata cgtgaaactg gacaaactgg
      601 gagagggcac ctatgccaca gtcttcaaag ggcgcagcaa actgacggag aaccttgtgg
      661 ccctgaaaga gatccggctg gagcacgagg agggagcgcc ctgcactgcc atccgagagg
      721 tgtctctgct gaagaacctg aagcacgcca atattgtgac cctgcatgac ctcatccaca
      781 cagatcggtc cctcaccctg gtgtttgagt acctggacag tgacctgaag cagtatctgg
      841 accactgtgg gaacctcatg agcatgcaca acgtcaagat tttcatgttc cagctgctcc
      901 ggggcctcgc ctactgtcac caccgcaaga tcctgcaccg ggacctgaag ccccagaacc
      961 tgctcatcaa cgagaggggg gagctgaagc tggccgactt tggactggcc agggccaagt
     1021 cagtgcccac aaagacttac tccaatgagg tggtgaccct gtggtacagg ccccccgatg
     1081 tgctgctggg atccacagag tactccaccc ccattgatat gtggggcgtg ggctgcatcc
     1141 actacgagat ggccacaggg aggcccctct tcccgggctc cacagtcaag gaggagctgc
     1201 acctcatctt tcgcctcctc gggaccccca cagaagagac gtggcccggc gtgaccgcct
     1261 tctctgagtt ccgcacctac agcttcccct gctacctccc gcagccgctc atcaaccacg
     1321 cgcccaggtt ggatacggat ggcatccacc tcctgagcag cctgctcctg tatgaatcca
     1381 agagtcgcat gtcagcagag gctgccctga gtcactccta cttccggtct ctgggagagc
     1441 gtgtgcacca gcttgaagac actgcctcca tcttctccct gaaggagatc cagctccaga
     1501 aggacccagg ctaccgaggc ttggccttcc agcagccagg acgagggaag aacaggcggc
     1561 agagcatctt ctgagccacg cccaccttgc tgtggccaag ggacaagaga tcacatggag
     1621 cacaaattcg ggtaggatgg agcctgtgtg gccctcggag gactgaagaa cgagggctga
     1681 cagccagcct ggaagaccgc ttggcagccc ttctggccac ggctgtttct tctttgtgct
     1741 tcccgtgtgc ctccccagta gccctcacct gcataccaac ccctccttta cccacgttgg
     1801 ggctggcata agctgcttcc ctgagaggac atgagggggg ggcggtcctc gtaccctctc
     1861 ccaccctggt gtttgggcac ctgcgtggga tgcacacgga tgacagaatc aaggcgccag
     1921 gatgggcact ctgccctgga tacaggctct accctcctcc cccaggacct gcctagtgcc
     1981 agtttggtag tccccctttc tggccccttg gagcccacac acgtttcatc tttttcccct
     2041 ctgagagcaa gaagagacat ggcatgttct ctgggaccct ggaatcctag gtacccacat
     2101 gtgtgccaaa gcctacccca cctggcaggt gtcccacagc aacagaagga atagtagtcc
     2161 ccactctttc catcagccct accctaccct cattccccga caccctctgg cttgaaccat
     2221 ggctgagcag tgccggcata cgctttgctg gcatgcttgg atgcccagct gtgtccagag
     2281 gtggcctggg accgccagtt gcacgcctgc cacctcagcc agcccccgcc cagctcatca
     2341 gtctgaatgg agttgcctta aattggcagg tggtaccgta ctcactgccc ttggagctgt
     2401 gaccggctcc tgcctgtcca ccccttcccg aggtggctcc tgcttacctt atcatcccag
     2461 ggctctgatt agccaggcct ggtcagggtc ctggggacgg cacccagata tgcagagtca
     2521 ccctgacact ggtgccaggc tgacctcagc tcccgaaggc tcgcacagcc tccccatcct
     2581 tccttcccag cccttgtggc tctgtccacc tgatcccaat accagcttcc cccagcccct
     2641 gccaccccag agggcggcca cgacagggag aggtgtagat gccaccatct gagggagagg
     2701 aacgtggaac aggagcaggc tctgatgctg agaggcttgc ctccgggggc tggaagcctg
     2761 ggtggccggg gcccctgaag aaggctcccc tctgtatccc ccaggtctcc tcaacactgg
     2821 gctgatcctg aatggcacag gccaagggga ggccagcctc gcctttctac ccaggccccc
     2881 tgccctgccc acctcaggcc cccaccctcc actcctcccc acggtactgt gaacgtcgtg
     2941 tgactcagtg cagagacaga taatatattt aattcatgta cagaaa
//
