LOCUS       NM_003718               9525 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens cyclin dependent kinase 13 (CDK13), transcript variant
            1, mRNA.
ACCESSION   NM_003718
VERSION     NM_003718.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Zhang,Y., Chen,X.N., Zhang,H., Wen,J.K., Gao,H.T., Shi,B.,
            Wang,D.D., Han,Z.W., Gu,J.F., Zhao,C.M., Xue,W.Y., Zhang,Y.P.,
            Qu,C.B. and Yang,Z.
  TITLE     CDK13 promotes lipid deposition and prostate cancer progression by
            stimulating NSUN5-mediated m5C modification of ACC1 mRNA
  JOURNAL   Cell Death Differ 30 (12), 2462-2476 (2023)
   PUBMED   37845385
  REMARK    GeneRIF: CDK13 promotes lipid deposition and prostate cancer
            progression by stimulating NSUN5-mediated m5C modification of ACC1
            mRNA.
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Morison,L.D., van Reyk,O., Forbes,E., Rouxel,F., Faivre,L.,
            Bruinsma,F., Vincent,M., Jacquemont,M.L., Dykzeul,N.L.,
            Genevieve,D., Amor,D.J. and Morgan,A.T.
  TITLE     CDK13-related disorder: a deep characterization of speech and
            language abilities and addition of 33 novel cases
  JOURNAL   Eur J Hum Genet 31 (7), 793-804 (2023)
   PUBMED   36599938
  REMARK    GeneRIF: CDK13-related disorder: a deep characterization of speech
            and language abilities and addition of 33 novel cases.
            Erratum:[Eur J Hum Genet. 2023 Dec 8. doi:
            10.1038/s41431-023-01515-5.. PMID: 38066173]
REFERENCE   3  (bases 1 to 9525)
  AUTHORS   Panzeri,V., Pieraccioli,M., Cesari,E., de la Grange,P. and Sette,C.
  TITLE     CDK12/13 promote splicing of proximal introns by enhancing the
            interaction between RNA polymerase II and the splicing factor SF3B1
  JOURNAL   Nucleic Acids Res 51 (11), 5512-5526 (2023)
   PUBMED   37026485
  REMARK    GeneRIF: CDK12/13 promote splicing of proximal introns by enhancing
            the interaction between RNA polymerase II and the splicing factor
            SF3B1.
REFERENCE   4  (bases 1 to 9525)
  AUTHORS   Insco,M.L., Abraham,B.J., Dubbury,S.J., Kaltheuner,I.H., Dust,S.,
            Wu,C., Chen,K.Y., Liu,D., Bellaousov,S., Cox,A.M., Martin,B.J.E.,
            Zhang,T., Ludwig,C.G., Fabo,T., Modhurima,R., Esgdaille,D.E.,
            Henriques,T., Brown,K.M., Chanock,S.J., Geyer,M., Adelman,K.,
            Sharp,P.A., Young,R.A., Boutz,P.L. and Zon,L.I.
  TITLE     Oncogenic CDK13 mutations impede nuclear RNA surveillance
  JOURNAL   Science 380 (6642), eabn7625 (2023)
   PUBMED   37079685
  REMARK    GeneRIF: Oncogenic CDK13 mutations impede nuclear RNA surveillance.
REFERENCE   5  (bases 1 to 9525)
  AUTHORS   Bartkowiak,B., Liu,P., Phatnani,H.P., Fuda,N.J., Cooper,J.J.,
            Price,D.H., Adelman,K., Lis,J.T. and Greenleaf,A.L.
  TITLE     CDK12 is a transcription elongation-associated CTD kinase, the
            metazoan ortholog of yeast Ctk1
  JOURNAL   Genes Dev 24 (20), 2303-2316 (2010)
   PUBMED   20952539
REFERENCE   6  (bases 1 to 9525)
  AUTHORS   Malumbres,M., Harlow,E., Hunt,T., Hunter,T., Lahti,J.M.,
            Manning,G., Morgan,D.O., Tsai,L.H. and Wolgemuth,D.J.
  TITLE     Cyclin-dependent kinases: a family portrait
  JOURNAL   Nat Cell Biol 11 (11), 1275-1276 (2009)
   PUBMED   19884882
REFERENCE   7  (bases 1 to 9525)
  AUTHORS   Even,Y., Durieux,S., Escande,M.L., Lozano,J.C., Peaucellier,G.,
            Weil,D. and Geneviere,A.M.
  TITLE     CDC2L5, a Cdk-like kinase with RS domain, interacts with the
            ASF/SF2-associated protein p32 and affects splicing in vivo
  JOURNAL   J Cell Biochem 99 (3), 890-904 (2006)
   PUBMED   16721827
  REMARK    GeneRIF: Data demonstrate that CDC2L5 is located in the
            nucleoplasm, where it directly interacts with the
            ASF/SF2-associated protein p32, a protein involved in splicing
            regulation.
REFERENCE   8  (bases 1 to 9525)
  AUTHORS   Marques,F., Moreau,J.L., Peaucellier,G., Lozano,J.C., Schatt,P.,
            Picard,A., Callebaut,I., Perret,E. and Geneviere,A.M.
  TITLE     A new subfamily of high molecular mass CDC2-related kinases with
            PITAI/VRE motifs
  JOURNAL   Biochem Biophys Res Commun 279 (3), 832-837 (2000)
   PUBMED   11162436
REFERENCE   9  (bases 1 to 9525)
  AUTHORS   Bostwick,B.
  TITLE     CDK13-Related Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30702837
REFERENCE   10 (bases 1 to 9525)
  AUTHORS   Lapidot-Lifson,Y., Patinkin,D., Prody,C.A., Ehrlich,G., Seidman,S.,
            Ben-Aziz,R., Benseler,F., Eckstein,F., Zakut,H. and Soreq,H.
  TITLE     Cloning and antisense oligodeoxynucleotide inhibition of a human
            homolog of cdc2 required in hematopoiesis
  JOURNAL   Proc Natl Acad Sci U S A 89 (2), 579-583 (1992)
   PUBMED   1731328
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC072061.8, AK092534.1,
            AJ297709.1, BC080601.1, BC001274.1, AK223053.1, CR748837.1,
            BM699295.1, BM727913.1, BU686489.1 and AC006023.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003718.4.
            
            Summary: The protein encoded by this gene is a member of the
            cyclin-dependent serine/threonine protein kinase family. Members of
            this family are well known for their essential roles as master
            switches in cell cycle control. The exact function of this protein
            has not yet been determined, but it may play a role in mRNA
            processing and may be involved in regulation of hematopoiesis.
            Alternatively spliced transcript variants have been
            described.[provided by RefSeq, Dec 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ297709.1, SRR1803611.88281.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000181839.10/ ENSP00000181839.4
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               AC072061.8         83835-83938
            105-693             AK092534.1         1-589
            694-1015            AJ297709.1         440-761
            1016-1016           BC080601.1         1235-1235
            1017-2396           AJ297709.1         763-2142
            2397-2485           BC001274.1         563-651
            2486-3893           AJ297709.1         2232-3639
            3894-3894           AK223053.1         763-763
            3895-5500           AJ297709.1         3641-5246
            5501-5957           CR748837.1         245-701
            5958-6019           BM699295.1         239-300
            6020-6588           BM727913.1         1-569
            6589-7079           BU686489.1         17-507              c
            7080-9525           AC006023.2         63062-65507
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.1"
     gene            1..9525
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="cyclin dependent kinase 13"
                     /db_xref="GeneID:8621"
                     /db_xref="HGNC:HGNC:1733"
                     /db_xref="MIM:603309"
     exon            1..1597
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     CDS             387..4925
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /EC_number="2.7.11.23"
                     /EC_number="2.7.11.22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CDC2-related protein kinase 5; cholinesterase-related cell
                     division controller; cell division protein kinase 13; cell
                     division cycle 2-like protein kinase 5"
                     /codon_start=1
                     /product="cyclin-dependent kinase 13 isoform 1"
                     /protein_id="NP_003709.3"
                     /db_xref="CCDS:CCDS5461.1"
                     /db_xref="GeneID:8621"
                     /db_xref="HGNC:HGNC:1733"
                     /db_xref="MIM:603309"
                     /translation="MPSSSDTALGGGGGLSWAEKKLEERRKRRRFLSPQQPPLLLPLL
                     QPQLLQPPPPPPPLLFLAAPGTAAAAAAAAAASSSCFSPGPPLEVKRLARGKRRAGGR
                     QKRRRGPRAGQEAEKRRVFSLPQPQQDGGGGASSGGGVTPLVEYEDVSSQSEQGLLLG
                     GASAATAATAAGGTGGSGGSPASSSGTQRRGEGSERRPRRDRRSSSGRSKERHREHRR
                     RDGQRGGSEASKSRSRHSHSGEERAEVAKSGSSSSSGGRRKSASATSSSSSSRKDRDS
                     KAHRSRTKSSKEPPSAYKEPPKAYREDKTEPKAYRRRRSLSPLGGRDDSPVSHRASQS
                     LRSRKSPSPAGGGSSPYSRRLPRSPSPYSRRRSPSYSRHSSYERGGDVSPSPYSSSSW
                     RRSRSPYSPVLRRSGKSRSRSPYSSRHSRSRSRHRLSRSRSRHSSISPSTLTLKSSLA
                     AELNKNKKARAAEAARAAEAAKAAEATKAAEAAAKAAKASNTSTPTKGNTETSASASQ
                     TNHVKDVKKIKIEHAPSPSSGGTLKNDKAKTKPPLQVTKVENNLIVDKATKKAVIVGK
                     ESKSAATKEESVSLKEKTKPLTPSIGAKEKEQHVALVTSTLPPLPLPPMLPEDKEADS
                     LRGNISVKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTATQLHSKRRPKI
                     CGPRYGETKEKDIDWGKRCVDKFDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDN
                     EKEGFPITAIREIKILRQLTHQSIINMKEIVTDKEDALDFKKDKGAFYLVFEYMDHDL
                     MGLLESGLVHFNENHIKSFMRQLMEGLDYCHKKNFLHRDIKCSNILLNNRGQIKLADF
                     GLARLYSSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIF
                     QANQELAQLELISRICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAAL
                     DLFDYMLALDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRRQKQ
                     MGMTDDVSTIKAPRKDLSLGLDDSRTNTPQGVLPSSQLKSQGSSNVAPVKTGPGQHLN
                     HSELAILLNLLQSKTSVNMADFVQVLNIKVNSETQQQLNKINLPAGILATGEKQTDPS
                     TPQQESSKPLGGIQPSSQTIQPKVETDAAQAAVQSAFAVLLTQLIKAQQSKQKDVLLE
                     ERENGSGHEASLQLRPPPEPSTPVSGQDDLIQHQDMRILELTPEPDRPRILPPDQRPP
                     EPPEPPPVTEEDLDYRTENQHVPTTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGG
                     IDYQAGDTYVSTSDYKDNFGSSSFSSAPYVSNDGLGSSSAPPLERRSFIGNSDIQSLD
                     NYSTASSHSGGPPQPSAFSESFPSSVAGYGDIYLNAGPMLFSGDKDHRFEYSHGPIAV
                     LANSSDPSTGPESTHPLPAKMHNYNYGGNLQENPSGPSLMHGQTWTSPAQGPGYSQGY
                     RGHISTSTGRGRGRGLPY"
     exon            1598..2257
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2428
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2568
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..2739
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2929
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            2930..2986
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3088
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            3089..3166
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3283
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            3284..3415
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            3416..3621
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            3622..4074
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     exon            4075..9525
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5454..5459
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="hexamer: AATAAA"
     polyA_site      5477
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="major polyA site"
     regulatory      7055..7060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="hexamer: ATTAAA"
     polyA_site      7079
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="major polyA site"
     regulatory      8986..8991
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="hexamer: AATAAA"
     polyA_site      9007
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
     regulatory      9503..9508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
                     /note="hexamer: AATAAA"
     polyA_site      9525
                     /gene="CDK13"
                     /gene_synonym="CDC2L; CDC2L5; CHDFIDD; CHED; hCDK13"
ORIGIN      
        1 acttccgcgt tgcgctgccc gagccgagag cgcggccaag gccgctcccc cacccccggg
       61 ggcacttgga ggactcggga ctcccccgca ggtcagcgcc cggcgcatct ggtgttttcg
      121 ctgccgagga taggacgacg agcgcaatcg ggagctccgc cgcccggatt cctgcttccc
      181 tggggcccgg aggctgctgc gtaccccact gtgacctgga acccagggac ccgagtcccg
      241 acccggatta tcgtggcgct tttcccggcc ggctctggtg ctcggtgtcc ctccgccgcc
      301 gctcccgttt ccggcggggg agatggccag gatctgaccc gggaggaggc cgcacccgcg
      361 ccgcgctctg cggctggctc taggcgatgc cgagcagctc ggacacggcg ctggggggag
      421 gcgggggcct gagctgggcg gagaagaagt tggaggaacg ccgcaagcgg aggcgattcc
      481 tgtcccctca gcagccgccg ctgctgttgc cgctcctgca gccgcagctc ctgcaaccgc
      541 cgccgccccc gccgcctctg ctcttcctgg ctgctcccgg cacggccgcc gccgcagccg
      601 ccgccgccgc ggcctcctcc tcttgcttca gcccgggccc ccctctggag gtcaagcggc
      661 tggcgagagg caagaggcgc gcaggagggc ggcagaagcg gcgtcgcggg ccccgcgccg
      721 ggcaggaggc ggagaagcgt cgggtcttct cgctgcccca gccgcagcag gacggcggtg
      781 gcggtgctag tagcggcggg ggtgtgaccc cgctggtgga atacgaggat gtgagctccc
      841 agtccgagca ggggctgctg ctgggggggg ccagcgcggc aacggcggcg acggctgccg
      901 ggggaacggg gggcagcggc gggagtccgg cctcctcctc cggcacccag cggcgcgggg
      961 aggggtcgga gcgcaggccc cgccgggacc gccgcagcag cagtggccgc agcaaggagc
     1021 gccaccgcga gcaccggcgg cgggatgggc agcgcggtgg cagcgaggcc tccaagtccc
     1081 gcagccgcca cagccacagc ggcgaggaac gggccgaggt cgccaagagc ggcagcagca
     1141 gcagcagcgg cggccgccgg aaaagcgctt cggccacatc cagcagcagt agcagccgca
     1201 aggaccggga ctcgaaggcc caccgcagcc ggactaagtc gtccaaggag ccgccttcgg
     1261 cctacaagga accgcccaag gcctaccggg aggacaagac cgagcctaag gcctacaggc
     1321 ggcggcggtc cctcagccca ctgggaggcc gggacgacag cccggtgtcc cacagggcct
     1381 ctcagagcct gaggagccgc aagtccccca gcccggcagg aggtggcagc agcccctatt
     1441 ctcggcggct gccgcgctcc ccgagcccct acagtcgccg ccgctccccc agctacagcc
     1501 gccacagctc ctacgagcgg ggcggcgacg tgtcccctag tccctacagc agcagcagct
     1561 ggcgccgctc tcgcagtccc tacagccctg tgctcagacg gtctggaaaa tcccgaagca
     1621 gaagcccgta ttcatctagg cattcaagat ctcgtagcag gcacagattg tctagatcca
     1681 gaagtcgtca ttctagtatt tctcctagca cactaactct gaagagtagc ctggcagctg
     1741 aattgaacaa gaataaaaaa gcacgagcag cagaggcagc aagagccgca gaagcagcga
     1801 aagctgcaga agcaactaag gctgctgagg ctgctgccaa ggctgcaaaa gcttcaaaca
     1861 cttctacacc taccaagggg aacacggaaa ctagtgccag tgcatcacaa acaaaccatg
     1921 tgaaggatgt gaagaaaatt aaaattgaac atgcaccttc tccctcaagt ggtggaactt
     1981 taaaaaatga caaagcaaaa acaaagccac ctcttcaggt aacgaaggtg gaaaataatt
     2041 tgattgtaga taaagccacc aagaaagcag tcatagttgg aaaggagagt aaatctgctg
     2101 ctacaaagga ggaatcagta tctcttaaag agaaaaccaa accacttaca ccaagcatag
     2161 gagccaagga gaaggagcaa catgtagctt tagtcacctc tacattacca ccgttacctt
     2221 tgcctcccat gctgcctgaa gataaagaag ctgatagctt acgaggaaat atttcagtaa
     2281 aagcagttaa aaaagaagta gaaaagaaac tccgatgtct tcttgctgat ttaccgctgc
     2341 cccctgagct accaggagga gatgatcttt caaagagtcc agaggaaaag aaaacagcaa
     2401 cacagttaca tagtaaaagg aggcctaaaa tatgtgggcc tcgctatggt gaaaccaaag
     2461 aaaaagatat tgactgggga aaacgctgcg tggataaatt tgatatcatc ggaattattg
     2521 gagaaggtac ttacggacaa gtttacaaag ccagggataa agacactgga gaaatggtag
     2581 ccttaaaaaa agtacgtctg gataatgaaa aggaaggctt tccaattaca gcaattcgag
     2641 aaattaaaat tctccggcag cttacccatc agagtattat caatatgaag gaaatagtga
     2701 ctgataaaga agatgctttg gatttcaaga aggacaaagg tgcattttat ctggtgtttg
     2761 aatatatgga ccatgatctg atgggactac tggaatcagg cttggttcat tttaatgaaa
     2821 atcacataaa gtcatttatg agacagctca tggagggtct ggattattgt cataagaaga
     2881 actttttgca tagagatatt aaatgttcca atatccttct aaataataga gggcagataa
     2941 aacttgcaga ctttggactt gctcgattgt atagctcaga agaaagtcgg ccgtatacta
     3001 acaaggtaat tactttatgg taccgtccac ctgaactgct actgggagaa gaacgataca
     3061 caccagccat tgatgtatgg agctgtggct gtatccttgg cgaactcttc actaaaaaac
     3121 ctatatttca agcaaatcag gaacttgcac aactagaatt aataagccga atatgtggga
     3181 gtccatgtcc tgcagtgtgg cctgatgtaa tcaaactacc atatttcaac accatgaaac
     3241 caaagaagca atatcgtcga aagttaagag aagaatttgt ttttattcct gcagctgcgc
     3301 tagacttatt tgattacatg cttgccttgg atcctagtaa gcgctgcact gctgaacagg
     3361 ctcttcagtg cgagttcctc cgagatgtgg aaccctcaaa aatgcctcca ccagatctcc
     3421 ctttatggca agattgtcat gagttatgga gtaaaaagcg aagaagacag aagcagatgg
     3481 gcatgactga tgatgtttcc acaattaaag cccccaggaa ggacttgtct ctgggcttgg
     3541 atgacagcag aaccaacaca ccccagggtg tgctgccatc ttcacagctg aaatctcagg
     3601 gcagctcaaa tgtggcacct gtaaaaacag gccctggaca gcacttaaac cacagtgaat
     3661 tggcaattct actaaaccta ctacaatcta aaacaagtgt taatatggct gattttgtcc
     3721 aagtgttgaa cattaaggta aactctgaga ctcaacagca gctaaataaa ataaaccttc
     3781 ctgctggaat tttggcaaca ggtgaaaaac agacagatcc atcaacacca caacaggagt
     3841 cttcgaaacc gttgggagga attcagcctt cttctcagac catccagcct aaagtggaga
     3901 ctgatgctgc ccaggcggct gtgcagagtg catttgcagt tctgttgact cagttaataa
     3961 aggctcagca gtcaaagcag aaagatgtgc tactagaaga gagggaaaat ggatcgggac
     4021 atgaagcgtc attacaactc aggccacctc cagaacctag cactccggtg tcgggacaag
     4081 atgacctcat ccagcatcaa gatatgagga tcttggagct aacgccagaa ccagaccggc
     4141 ctcgaattct gcctcctgac caacgacctc ccgagcctcc tgaaccacca ccagtcactg
     4201 aggaagatct agattatcgg acagaaaacc agcatgtacc caccaccagt tcttcattaa
     4261 ctgaccctca tgccggagtg aaggcagccc tgttacagct gcttgctcag catcagcccc
     4321 aggatgaccc caaaagagaa ggtgggattg attatcaagc aggagacact tacgtgtcca
     4381 cttcagacta caaggacaac tttggatcct cttctttctc ttctgctcct tatgttagca
     4441 atgatggtct aggaagcagt tctgctccac cactagaacg acgtagtttc attggaaatt
     4501 cagatattca gtctttggat aactacagta ctgcttcatc tcattctggt ggtccacctc
     4561 agccttctgc cttttctgag tcatttccca gttcagtagc tggatatgga gacatttacc
     4621 tcaatgctgg tcccatgttg tttagtggag acaaggacca tagatttgaa tatagccatg
     4681 gtcctattgc agtcctggca aacagcagtg acccttccac ggggccagag agtactcatc
     4741 ctttgccagc aaagatgcac aactataact atggtggtaa cttacaggaa aatccgagtg
     4801 gccccagcct catgcatgga cagacctgga cttctcctgc ccaaggacct ggatattcac
     4861 aaggatacag gggacatatt agcacatcaa ctggcagagg cagaggcaga gggttaccat
     4921 actgagtatc tgtttttcct caggcacatc atttttatct ggaaagactt ttctagctgc
     4981 aatttaaggc agcaatccaa gagacttgaa taataataat tcaacaacag ctttattttt
     5041 atgtggagaa gggtcttgca tacaatagtt taaaaaagac aaaaaaaacc tttgcttaaa
     5101 ttcatgctgt tctaaaaact agatcgattg tacatcttca caaattctag ttaacaattt
     5161 tattttgtat tcttgcagtt ttaagtggat gctaatttta ggggcataag ccttttatgg
     5221 ccctcttgca gatcttctga actatgcaca tttgtgcttt ttttgtaagt ttggaccaac
     5281 ttttatgtaa caaacagccc ctccccacct ccagttttac aacaatcaga aagggcactg
     5341 atttatttgg tatttttctt tttacaaagc tacctttagt caaaggtcac tgtcagtctt
     5401 tgcacctgct ttcagtgtta ttgtgaaagg tgtactttgt gctcatttca gaaaataaaa
     5461 cacaaccttt ctcttgatgc aacagtttta taaaaaaaaa aatggtcaac gttatttttg
     5521 ttttgttttg cagattatca aagcctagca aaatgcattt aaatgaaata gtgggtttta
     5581 tatgaaaact atgggtgggg tggggaggga aagtaagtgc cttaacaggt aagcttaagg
     5641 tctgaaaaaa atagttaact tttaccccca tttgtctttt aaagggaatc aatgcattaa
     5701 aaaggcaaga aactctgaag tttcaggggc tgcctgttca gttcctgtta ggtccattcc
     5761 ctctgcttgt cacactttct ccttttctgc ctgtctttgg gctgtgttgc ctttcactac
     5821 caccttctct tgatagggga gtgagagcaa agaaagggag tagaactgca atcagtacat
     5881 attgtttgag aagttctaca aagttttgtc atttaaatgg ttgaaagtaa tagctccagg
     5941 agaattttag agtaccattt tctttcaggc tgccatatta tccctgtttc catttgccat
     6001 ggataattgg agggtcatgg tatagaagca agagttaata acagaacttg cctaaatgtg
     6061 ttaaagttca tgggggaggg gtggcaaata ccgtatttta agttatgcaa ttgccctttt
     6121 aagaaagtca attctattca cttgttaact agaaacattg cttagattga atttattgaa
     6181 gcaaaaagaa aaggcaatga gagagaactt ttatcttcct tcctgtcaca ttctgtaaag
     6241 ttgattatga cttctgccag acttagagca acaactttag ttcattttaa catttttttt
     6301 taatgaagag catcaaagaa tggagtagtc cttatttaaa ctgtaatggt gtcaagtctc
     6361 actgtgtgca cactctcaca catacacaca caattatact aattctagaa aggaaccaaa
     6421 ggtaaataac cagtgtttcc attatcacta aatggaaaaa ttgactggaa gttagagttg
     6481 tgccaattaa tcattttgtt catcaactgt tctggtaact tatttttttt tattggaatc
     6541 ttaaattgga acaactgtta cagaatgtat attgctccag ttctaaagcc agttaaccct
     6601 gtgccctcta acttatgaac cttcatttca aaacagtatg tgggaagtcc tgtagtttga
     6661 taagagtaaa attattttaa agaggataat ttaccatttt actactataa aataagttga
     6721 tatcagttga ccattttatt ttttgatgga ctctttttgt tgggagaaag aaatgaatgg
     6781 aagaaaaaaa tatgttgctt ttatttgaat attgattact aacaacttat aaactacatt
     6841 gcaggaaatg aagaaatatt ttgaaattag caaaatagca acagtattca agtatctgaa
     6901 attttaatat ggtgagtctt agagctttag actttcctat aagtgacagt atctaagtta
     6961 ttcttaatag tcacacttga taattctgct gaccataagc agctaaccta cagttgaaga
     7021 tatttatgtg acatgaatag cctgtgtttt aaaaattaaa tattttatat aaaattgaac
     7081 tgtgtttgga tagtttttct aatcaaacat ttcagttata tatttatgtt gggagagtgg
     7141 ctgggaggac agggaaattg ttgctttgac attgaaaatt tgaactagaa cttctaatat
     7201 tatccttaca cagcactact gtgagacata ctagtctttt atatgtcact tagaagaaaa
     7261 aatgttgcca attaagccac aatacaatgc taagacgtca tcaattgtaa gctgcatccc
     7321 agattcagac atattatatg aaaatatgtc ttataattga tgaaatatga tagtaaaaaa
     7381 ttttaatgct tcccttcata gcatttaata ttttaattca gtgaatttgg attaatagaa
     7441 caaagttggg aaatcactag ttctgtaggt tataacagca gtcatcgctg aaaatttgag
     7501 tgaaatctaa agaacacaaa atattgctta aattttttgg aaaaaagcag gtaagtcaga
     7561 ctgcaagtta agaagacaaa aatataaaag atttgacctg ccaaaagaag aaagtattta
     7621 tcttctccca cgtgaaattc ccttttgtat ttactgacac agcaggtttt tctgcatgca
     7681 actcagtatg tttcctacgt cttcattatt tgttggagag ttgcaagtca tttgtgaaac
     7741 cttaaaatgc caattttaag gagttgtcat ttgtggaatg tgaaataagg taataattct
     7801 agctgtgatt cagagcttta ttctgtcagc aaatacatag tcagtgccta caacatgcca
     7861 ggtagtaatc tagtccttgg ggtacatcaa taaccgtcaa cagtgttaaa tcatactttc
     7921 agtgtgctga cagtgagcaa gattttttta aatgtctgga gaagttagtg gtatttgcta
     7981 ccctcagctg tatctaaatt gcacttaagt gaatgtaggg catgaaaatt tgagtatctt
     8041 tttgaaattt taaattgaaa tttggataga gatggttatg gagagaaatc aaacaactgg
     8101 aatagctgtt tgatatcact taaaagtgat aaaattttaa gttgaatctg gtcagtttgc
     8161 aatggcctat ttgtaagaaa tatcaagact tcttgagaaa aatgaaaagt gaatacataa
     8221 atgcttaaaa tctggtactt ctgagttaag gttttgctct ttgagcttaa tccaatttgg
     8281 gatgattttt catcctaggg ctttttgttt tcctttttta tttttatttt ttcttttttt
     8341 aggggaaggg gacttgcttt cttttccaaa aaggtgaatc cttcttgtag gacataggta
     8401 aaaaaaacaa agctgaaata tatgttttga atatagatag ctaattccct gggatataat
     8461 atcctttcaa tttttttttt ttttttggcc cagtctgcct tttgatgttt taaaagtctg
     8521 aacgagatgt cccagtaacc taaaattatc cagtcggtct tcttacttta caactaagaa
     8581 aaataaggct tagaaagagg gattgccaga aactttggca gctggattgc ctgtgcttgt
     8641 tcctctaagc catacctaaa ttctgcagta aatacttaac tttttaatag ggaaattgct
     8701 tcaagataac ttgaccagtg atacggtaaa ataattagac tattggacta atggtttaac
     8761 acaagtggct ttaaaaagtc tgcttaaaaa acaattttta tttagaaaaa atagaaaaat
     8821 aaaaacatct tcaaaattta ggagcctgaa ggggctgttt gtttcatata tggataatct
     8881 ttgaaaaggc aagtcctgta tgtatttttc atttgttgaa agaagattgg ttatcagtag
     8941 gcttgcaaac ataatttgct tttaagttct ttcaaggttt tatgcaataa aacctattga
     9001 tttggaactt taaaaaaaaa aacaacaaaa aaatactttc agggttttgt aatttcaagt
     9061 ggttttttaa ggggagcaat agtttgccat ttaccaaagg cttctccaga taatttctta
     9121 aatgtttcta cttaaaaata aaagctatta ataataagct gtcatgggat ccatttgaag
     9181 acagggaaaa tagaaaattt ttattgtaaa gggaagaact tatcctttta attttatgga
     9241 ctaacagagt ctgcaggtct taactcattt cagcctgtca aatgtgcaat taaaaatgaa
     9301 ttttctaatt gtattcaaat gaggctctat agtgaataca gaatcactct tctaagtttt
     9361 ttcccagtta atttgtttaa aagtgttgta ctctcttgca agaacgttta aaagttaagt
     9421 cttgtaactg ttaacatcta atgtattaat ataagccatt tgttttttac cattttttta
     9481 aggccgtatt caatagtctg taaataaatt gccgtaacac tccca
//
