LOCUS       NM_003504               1879 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens cell division cycle 45 (CDC45), transcript variant 2,
            mRNA.
ACCESSION   NM_003504
VERSION     NM_003504.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Schoch,K., Ruegg,M.S.G., Fellows,B.J., Cao,J., Uhrig,S.,
            Einsele-Scholz,S., Biskup,S., Hawarden,S.R.A., Salpietro,V.,
            Capra,V., Brown,C.M., Accogli,A., Shashi,V. and Bicknell,L.S.
  CONSRTM   Undiagnosed Diseases Network
  TITLE     A second hotspot for pathogenic exon-skipping variants in CDC45
  JOURNAL   Eur J Hum Genet 32 (7), 786-794 (2024)
   PUBMED   38467731
  REMARK    GeneRIF: A second hotspot for pathogenic exon-skipping variants in
            CDC45.
REFERENCE   3  (bases 1 to 1879)
  AUTHORS   Wu,L., Gao,G., Mi,H., Luo,Z., Wang,Z., Liu,Y., Wu,L., Long,H. and
            Shen,Y.
  TITLE     Validation of CDC45 as a novel biomarker for diagnosis and
            prognosis of gastric cancer
  JOURNAL   PeerJ 12, e17130 (2024)
   PUBMED   38515458
  REMARK    GeneRIF: Validation of CDC45 as a novel biomarker for diagnosis and
            prognosis of gastric cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1879)
  AUTHORS   Wu,T., Liao,L., Wu,T., Chen,S., Yi,Q. and Xu,M.
  TITLE     IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness
            by stabilizing CDC45 mRNA via m6A modification
  JOURNAL   Cell Cycle 22 (20), 2245-2263 (2023)
   PUBMED   37985379
  REMARK    GeneRIF: IGF2BP2 promotes glycolysis and hepatocellular carcinoma
            stemness by stabilizing CDC45 mRNA via m6A modification.
REFERENCE   5  (bases 1 to 1879)
  AUTHORS   Lu,Y., Chen,X., Liu,F., Yu,H., Zhang,Y., Du,K., Nan,Y. and Huang,Q.
  TITLE     Systematic pan-cancer analysis identifies CDC45 as having an
            oncogenic role in human cancers
  JOURNAL   Oncol Rep 48 (4) (2022)
   PUBMED   36082823
  REMARK    GeneRIF: Systematic pancancer analysis identifies CDC45 as having
            an oncogenic role in human cancers.
REFERENCE   6  (bases 1 to 1879)
  AUTHORS   Kukimoto,I., Igaki,H. and Kanda,T.
  TITLE     Human CDC45 protein binds to minichromosome maintenance 7 protein
            and the p70 subunit of DNA polymerase alpha
  JOURNAL   Eur J Biochem 265 (3), 936-943 (1999)
   PUBMED   10518787
REFERENCE   7  (bases 1 to 1879)
  AUTHORS   Shaikh,T.H., Gottlieb,S., Sellinger,B., Chen,F., Roe,B.A.,
            Oakey,R.J., Emanuel,B.S. and Budarf,M.L.
  TITLE     Characterization of CDC45L: a gene in the 22q11.2 deletion region
            expressed during murine and human development
  JOURNAL   Mamm Genome 10 (3), 322-326 (1999)
   PUBMED   10051334
REFERENCE   8  (bases 1 to 1879)
  AUTHORS   Leatherwood,J.
  TITLE     Emerging mechanisms of eukaryotic DNA replication initiation
  JOURNAL   Curr Opin Cell Biol 10 (6), 742-748 (1998)
   PUBMED   9914182
  REMARK    Review article
REFERENCE   9  (bases 1 to 1879)
  AUTHORS   McKie,J.M., Wadey,R.B., Sutherland,H.F., Taylor,C.L. and
            Scambler,P.J.
  TITLE     Direct selection of conserved cDNAs from the DiGeorge critical
            region: isolation of a novel CDC45-like gene
  JOURNAL   Genome Res 8 (8), 834-841 (1998)
   PUBMED   9724329
REFERENCE   10 (bases 1 to 1879)
  AUTHORS   Saha,P., Thome,K.C., Yamaguchi,R., Hou,Z., Weremowicz,S. and
            Dutta,A.
  TITLE     The human homolog of Saccharomyces cerevisiae CDC45
  JOURNAL   J Biol Chem 273 (29), 18205-18209 (1998)
   PUBMED   9660782
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC000087.2, AC000088.2 and
            AC000082.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 4, 2019 this sequence version replaced NM_003504.4.
            
            Summary: The protein encoded by this gene was identified by its
            strong similarity with Saccharomyces cerevisiae Cdc45, an essential
            protein required to the initiation of DNA replication. Cdc45 is a
            member of the highly conserved multiprotein complex including
            Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA
            polymerase, which is important for early steps of DNA replication
            in eukaryotes. This protein has been shown to interact with MCM7
            and DNA polymerase alpha. Studies of the similar gene in Xenopus
            suggested that this protein play a pivotal role in the loading of
            DNA polymerase alpha onto chromatin. Alternate splicing results in
            multiple transcript variants. [provided by RefSeq, Jul 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF062495.1, BC010022.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263201.7/ ENSP00000263201.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC000087.2         22013-22112
            101-160             AC000087.2         22251-22310
            161-253             AC000087.2         23046-23138
            254-391             AC000087.2         24783-24920
            392-535             AC000087.2         25955-26098
            536-591             AC000087.2         36420-36475
            592-640             AC000087.2         38074-38122
            641-702             AC000087.2         39479-39540
            703-753             AC000087.2         41194-41244
            754-873             AC000088.2         9626-9745
            874-1005            AC000088.2         11650-11781
            1006-1104           AC000088.2         12030-12128
            1105-1266           AC000088.2         12794-12955
            1267-1405           AC000088.2         19013-19151
            1406-1489           AC000088.2         19229-19312
            1490-1608           AC000088.2         20791-20909
            1609-1685           AC000088.2         21081-21157
            1686-1751           AC000088.2         23108-23173
            1752-1879           AC000082.4         1343-1470
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.21"
     gene            1..1879
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /note="cell division cycle 45"
                     /db_xref="GeneID:8318"
                     /db_xref="HGNC:HGNC:1739"
                     /db_xref="MIM:603465"
     exon            1..100
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..1750
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /note="isoform 2 is encoded by transcript variant 2; cell
                     division cycle 45-like 2; CDC45 cell division cycle
                     45-like; CDC45-related protein; cell division control
                     protein 45 homolog; CDC45 cell division cycle 45 homolog;
                     human CDC45"
                     /codon_start=1
                     /product="cell division control protein 45 homolog isoform
                     2"
                     /protein_id="NP_003495.1"
                     /db_xref="CCDS:CCDS13762.1"
                     /db_xref="GeneID:8318"
                     /db_xref="HGNC:HGNC:1739"
                     /db_xref="MIM:603465"
                     /translation="MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDH
                     VQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHR
                     PVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRL
                     EEEIVEQTMRRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLW
                     WAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDL
                     RLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDI
                     SLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGS
                     GTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSL
                     MEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVG
                     IPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS
                     "
     exon            101..160
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            161..253
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            254..391
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            392..535
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..591
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            592..640
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            641..702
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            703..753
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            754..873
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1005
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1104
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1266
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1405
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1489
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1608
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1685
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1751
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1879
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1853..1858
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /note="hexamer: AATAAA"
     polyA_site      1879
                     /gene="CDC45"
                     /gene_synonym="CDC45L; CDC45L2; MGORS7; PORC-PI-1"
                     /note="major polyA site"
ORIGIN      
        1 gggctcttgg tacctcagcg cgagcgccag gcgtccggcc gccgtggcta tgttcgtgtc
       61 cgatttccgc aaagagttct acgaggtggt ccagagccag agggtccttc tcttcgtggc
      121 ctcggacgtg gatgctctgt gtgcgtgcaa gatccttcag gccttgttcc agtgtgacca
      181 cgtgcaatat acgctggttc cagtttctgg gtggcaagaa cttgaaactg catttcttga
      241 gcataaagaa cagtttcatt attttattct cataaactgt ggagctaatg tagacctatt
      301 ggatattctt caacctgatg aagacactat attctttgtg tgtgacaccc ataggccagt
      361 caatgtcgtc aatgtataca acgataccca gatcaaatta ctcattaaac aagatgatga
      421 ccttgaagtt cccgcctatg aagacatctt cagggatgaa gaggaggatg aagagcattc
      481 aggaaatgac agtgatgggt cagagccttc tgagaagcgc acacggttag aagaggagat
      541 agtggagcaa accatgcgga ggaggcagcg gcgagagtgg gaggcccgga gaagagacat
      601 cctctttgac tacgagcagt atgaatatca tgggacatcg tcagccatgg tgatgtttga
      661 gctggcttgg atgctgtcca aggacctgaa tgacatgctg tggtgggcca tcgttggact
      721 aacagaccag tgggtgcaag acaagatcac tcaaatgaaa tacgtgactg atgttggtgt
      781 cctgcagcgc cacgtttccc gccacaacca ccggaacgag gatgaggaga acacactctc
      841 cgtggactgc acacggatct cctttgagta tgacctccgc ctggtgctct accagcactg
      901 gtccctccat gacagcctgt gcaacaccag ctataccgca gccaggttca agctgtggtc
      961 tgtgcatgga cagaagcggc tccaggagtt ccttgcagac atgggtcttc ccctgaagca
     1021 ggtgaagcag aagttccagg ccatggacat ctccttgaag gagaatttgc gggaaatgat
     1081 tgaagagtct gcaaataaat ttgggatgaa ggacatgcgc gtgcagactt tcagcattca
     1141 ttttgggttc aagcacaagt ttctggccag cgacgtggtc tttgccacca tgtctttgat
     1201 ggagagcccc gagaaggatg gctcagggac agatcacttc atccaggctc tggacagcct
     1261 ctccaggagt aacctggaca agctgtacca tggcctggaa ctcgccaaga agcagctgcg
     1321 agccacccag cagaccattg ccagctgcct ttgcaccaac ctcgtcatct cccaggggcc
     1381 tttcctgtac tgctctctca tggagggcac tccagatgtc atgctgttct ctaggccggc
     1441 atccctaagc ctgctcagca aacacctgct caagtccttt gtgtgttcga caaagaaccg
     1501 gcgctgcaaa ctgctgcccc tggtgatggc tgcccccctg agcatggagc atggcacagt
     1561 gaccgtggtg ggcatccccc cagagaccga cagctcggac aggaagaact tttttgggag
     1621 ggcgtttgag aaggcagcgg aaagcaccag ctcccggatg ctgcacaacc attttgacct
     1681 ctcagtaatt gagctgaaag ctgaggatcg gagcaagttt ctggacgcac ttatttccct
     1741 cctgtcctag gaatttgatt cttccagaat gaccttctta tttatgtaac tggctttcat
     1801 ttagattgta agttatggac atgatttgag atgtagaagc cattttttat taaataaaat
     1861 gcttatttta ggctccgtc
//
