LOCUS       NM_001394014           10978 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA),
            transcript variant G, mRNA.
ACCESSION   NM_001394014 XM_005273322
VERSION     NM_001394014.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Yu,S., Su,S., Wang,P., Li,J., Chen,C., Xin,H., Gong,Y., Wang,H.,
            Ye,X., Mao,L., Zhou,Z., Zhou,S., Hu,Z. and Huang,X.
  TITLE     Tumor-associated macrophage-induced circMRCKalpha encodes a peptide
            to promote glycolysis and progression in hepatocellular carcinoma
  JOURNAL   Cancer Lett 591, 216872 (2024)
   PUBMED   38642609
  REMARK    GeneRIF: Tumor-associated macrophage-induced circMRCKalpha encodes
            a peptide to promote glycolysis and progression in hepatocellular
            carcinoma.
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 10978)
  AUTHORS   Bruneau,A., Delaunay,J.L., Durand-Schneider,A.M., Vauthier,V., Ben
            Saad,A., Aoudjehane,L., El Mourabit,H., Morichon,R., Falguieres,T.,
            Gautheron,J., Housset,C. and Ait-Slimane,T.
  TITLE     MRCK-Alpha and Its Effector Myosin II Regulatory Light Chain Bind
            ABCB4 and Regulate Its Membrane Expression
  JOURNAL   Cells 11 (4), 617 (2022)
   PUBMED   35203270
  REMARK    GeneRIF: MRCK-Alpha and Its Effector Myosin II Regulatory Light
            Chain Bind ABCB4 and Regulate Its Membrane Expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10978)
  AUTHORS   Liu,J. and Dean,D.A.
  TITLE     Gene transfer of MRCKalpha rescues lipopolysaccharide-induced acute
            lung injury by restoring alveolar capillary barrier function
  JOURNAL   Sci Rep 11 (1), 20862 (2021)
   PUBMED   34675326
  REMARK    GeneRIF: Gene transfer of MRCKalpha rescues
            lipopolysaccharide-induced acute lung injury by restoring alveolar
            capillary barrier function.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10978)
  AUTHORS   Cervan-Martin,M., Bossini-Castillo,L., Rivera-Egea,R., Garrido,N.,
            Lujan,S., Romeu,G., Santos-Ribeiro,S., Castilla,J.A.,
            Gonzalvo,M.D.C., Clavero,A., Vicente,F.J., Guzman-Jimenez,A.,
            Burgos,M., Barrionuevo,F.J., Jimenez,R., Sanchez-Curbelo,J.,
            Lopez-Rodrigo,O., Peraza,M.F., Pereira-Caetano,I., Marques,P.I.,
            Carvalho,F., Barros,A., Bassas,L., Seixas,S., Goncalves,J.,
            Larriba,S., Lopes,A.M., Carmona,F.D. and Palomino-Morales,R.J.
  CONSRTM   IVIRMA Group, Lisbon Clinical Group
  TITLE     Effect and in silico characterization of genetic variants
            associated with severe spermatogenic disorders in a large Iberian
            cohort
  JOURNAL   Andrology 9 (4), 1151-1165 (2021)
   PUBMED   33784440
  REMARK    GeneRIF: Effect and in silico characterization of genetic variants
            associated with severe spermatogenic disorders in a large Iberian
            cohort.
REFERENCE   6  (bases 1 to 10978)
  AUTHORS   Ohashi,K., Nagata,K., Maekawa,M., Ishizaki,T., Narumiya,S. and
            Mizuno,K.
  TITLE     Rho-associated kinase ROCK activates LIM-kinase 1 by
            phosphorylation at threonine 508 within the activation loop
  JOURNAL   J Biol Chem 275 (5), 3577-3582 (2000)
   PUBMED   10652353
REFERENCE   7  (bases 1 to 10978)
  AUTHORS   Aoki,H., Hayashi,J., Moriyama,M., Arakawa,Y. and Hino,O.
  TITLE     Hepatitis C virus core protein interacts with 14-3-3 protein and
            activates the kinase Raf-1
  JOURNAL   J Virol 74 (4), 1736-1741 (2000)
   PUBMED   10644344
REFERENCE   8  (bases 1 to 10978)
  AUTHORS   Edwards,D.C., Sanders,L.C., Bokoch,G.M. and Gill,G.N.
  TITLE     Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase
            signalling to actin cytoskeletal dynamics
  JOURNAL   Nat Cell Biol 1 (5), 253-259 (1999)
   PUBMED   10559936
REFERENCE   9  (bases 1 to 10978)
  AUTHORS   Leung,T., Chen,X.Q., Tan,I., Manser,E. and Lim,L.
  TITLE     Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a
            Cdc42 effector in promoting cytoskeletal reorganization
  JOURNAL   Mol Cell Biol 18 (1), 130-140 (1998)
   PUBMED   9418861
REFERENCE   10 (bases 1 to 10978)
  AUTHORS   Zhao,Y., Loyer,P., Li,H., Valentine,V., Kidd,V. and Kraft,A.S.
  TITLE     Cloning and chromosomal location of a novel member of the myotonic
            dystrophy family of protein kinases
  JOURNAL   J Biol Chem 272 (15), 10013-10020 (1997)
   PUBMED   9092543
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL627308.20, AL451047.7 and
            AL353689.26.
            
            On Mar 26, 2021 this sequence version replaced XM_005273322.4.
            
            Summary: The protein encoded by this gene is a member of the
            serine/threonine protein kinase family. This kinase contains
            multiple functional domains. Its kinase domain is highly similar to
            that of the myotonic dystrophy protein kinase (DMPK). This kinase
            also contains a Rac interactive binding (CRIB) domain, and has been
            shown to bind CDC42. It may function as a CDC42 downstream effector
            mediating CDC42 induced peripheral actin formation, and promoting
            cytoskeletal reorganization. Multiple alternatively spliced
            transcript variants have been described. [provided by RefSeq, Sep
            2018].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000366766.8/ ENSP00000355728.5
            RefSeq Select criteria    :: based on manual assertion
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1488              AL627308.20        110307-111794       c
            1489-1580           AL627308.20        47366-47457         c
            1581-1664           AL627308.20        6438-6521           c
            1665-1760           AL451047.7         67914-68009         c
            1761-1909           AL451047.7         62143-62291         c
            1910-2003           AL451047.7         28900-28993         c
            2004-2204           AL451047.7         15716-15916         c
            2205-2453           AL451047.7         13846-14094         c
            2454-2533           AL451047.7         11302-11381         c
            2534-2700           AL451047.7         7933-8099           c
            2701-2823           AL353689.26        191295-191417       c
            2824-2957           AL353689.26        181990-182123       c
            2958-3200           AL353689.26        174857-175099       c
            3201-3311           AL353689.26        174498-174608       c
            3312-3559           AL353689.26        163178-163425       c
            3560-3665           AL353689.26        154072-154177       c
            3666-3790           AL353689.26        143079-143203       c
            3791-3896           AL353689.26        136445-136550       c
            3897-4045           AL353689.26        136050-136198       c
            4046-4137           AL353689.26        134394-134485       c
            4138-4214           AL353689.26        131963-132039       c
            4215-4319           AL353689.26        114067-114171       c
            4320-4403           AL353689.26        110113-110196       c
            4404-4509           AL353689.26        102317-102422       c
            4510-4646           AL353689.26        97657-97793         c
            4647-4786           AL353689.26        96841-96980         c
            4787-4868           AL353689.26        95520-95601         c
            4869-5085           AL353689.26        93484-93700         c
            5086-5148           AL353689.26        92594-92656         c
            5149-5742           AL353689.26        90843-91436         c
            5743-5840           AL353689.26        88241-88338         c
            5841-5925           AL353689.26        85449-85533         c
            5926-6049           AL353689.26        79113-79236         c
            6050-6167           AL353689.26        78266-78383         c
            6168-6285           AL353689.26        67180-67297         c
            6286-6443           AL353689.26        57009-57166         c
            6444-10978          AL353689.26        52051-56585         c
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.13"
     gene            1..10978
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /note="CDC42 binding protein kinase alpha"
                     /db_xref="GeneID:8476"
                     /db_xref="HGNC:HGNC:1737"
                     /db_xref="MIM:603412"
     exon            1..1488
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     CDS             1311..6575
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /EC_number="2.7.11.1"
                     /note="isoform G is encoded by transcript variant G; CDC42
                     binding protein kinase alpha (DMPK-like); ser-thr protein
                     kinase related to the myotonic dystrophy protein kinase;
                     ser-thr protein kinase PK428; myotonic dystrophy
                     kinase-related CDC42-binding protein kinase alpha;
                     serine/threonine-protein kinase MRCK alpha; myotonic
                     dystrophy protein kinase-like alpha; CDC42 binding protein
                     kinase beta"
                     /codon_start=1
                     /product="serine/threonine-protein kinase MRCK alpha
                     isoform G"
                     /protein_id="NP_001380943.1"
                     /db_xref="CCDS:CCDS91169.1"
                     /db_xref="GeneID:8476"
                     /db_xref="HGNC:HGNC:1737"
                     /db_xref="MIM:603412"
                     /translation="MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNN
                     SPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKV
                     FAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGG
                     DLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLA
                     DFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEML
                     YGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGI
                     EDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAF
                     SGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKR
                     LEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSH
                     LEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELK
                     DAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRT
                     ERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEH
                     QQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILK
                     DKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQ
                     QLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSS
                     LGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKL
                     KDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPV
                     ENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPP
                     KRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPP
                     EQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKAS
                     QPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILML
                     ADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHER
                     IALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSAL
                     DGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEI
                     QVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAIC
                     AVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDI
                     FDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNS
                     RKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDG
                     IQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSAL
                     KREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSN
                     LSSPPSPASPRKTKSLSLESTDRGSWDP"
     exon            1489..1580
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1664
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1760
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            1761..1909
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2003
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2204
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2453
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2533
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..2700
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2823
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2957
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            2958..3200
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            3201..3311
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            3312..3559
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3665
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            3666..3790
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            3791..3896
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            3897..4045
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4046..4137
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4138..4214
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4215..4319
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4320..4403
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4404..4509
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4510..4646
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4647..4786
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4787..4868
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            4869..5085
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            5086..5148
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            5149..5742
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            5743..5840
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            5841..5925
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            5926..6049
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            6050..6167
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            6168..6285
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            6286..6443
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     exon            6444..10978
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8099..8104
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /note="hexamer: AATAAA"
     polyA_site      8122
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
     regulatory      10959..10964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /note="hexamer: AATAAA"
     polyA_site      10978
                     /gene="CDC42BPA"
                     /gene_synonym="MRCK; MRCKA; MRCKalpha; PK428"
                     /note="major polyA site"
ORIGIN      
        1 atattggatc cagactcgtc cgcaagcctc cgcctctgtg cggcgggact ggaggagcct
       61 cgctgagccc agggcgcgag cgcgagagga gagggaaggc gggggagggc tggaagggag
      121 aggaagggag tggttgggag ccgggctgcc gcagcctcta gtctcctcag ccgcggaagc
      181 gacccctcct cctgcgccgg gccgcctccc tcctcgctgt ggaaagatgc ccttagccca
      241 ggggtgtgaa gaagggggag aagtagctgc cagagccgcc cgcgccgccg ccgcagctgc
      301 tgctgcttgt gtcctttgaa ttcagagaag cacccccccg cctggggcgt cgggagcctc
      361 cggcgcggcg gcccggtgcg ggtgtcgggg agaccgggct ctctgcccgg cgcggcgcgg
      421 cgcggctcgg cccacgagcg accaccgaca tggagtgggc tcgggcggcc aagtagccgc
      481 ttctccggag cccggtgcca gtgccgcccg cagcccgcct tccacccccg gccgcgccgc
      541 cggtcaggcc ctagggtgaa gccgggagga aaatgaagag ttttcaccgg aatccgttga
      601 aaataggact gactgcaaag ccttaaagaa agaaggacct cgggaggaga aacgaaaagc
      661 cgcctccggg caagacttgg cgtgctccga gccgaggggc tgcttcaggg acctcgcccc
      721 ctccctttcc cgctggagaa attgccgctg atgcattatc caagtggtgg ttgggaggat
      781 ttgcagcaac atttttggtt ttccctcccc cttctatgca ttctgttttt ttcctccctt
      841 ttctgttttt cttcttcccg ggaagtgaat tgctgatgca aatcggactt tattcattaa
      901 tgatgcaacc ggattcgttt caggattacg ttgcacgagt tgaattttga atgaaggaga
      961 agagtttttt tttttttttt taaagaagtg ttgactctct agttcgttgt acttttaatt
     1021 attattttat ttaaatatac gacttaattg tattctttta aaaatgcatt aagtatatat
     1081 tttatggtaa tttaccctca aaatatatgt atatgggtga aattgaagac gcttcagtta
     1141 agtgaggtta ctggtgtgtt ggatgtttaa ttcagcacca gcattgcatg acagttgttt
     1201 gaataacaag tggtttattt ttaaaaccat accttttaaa atttaggttc agataatagt
     1261 aaaagtcatc ataataattt aaaggaaaac cagcagaaat cgaagcaaac atgtctggag
     1321 aagtgcgttt gaggcagttg gagcagttta ttttggacgg gcccgctcag accaatgggc
     1381 agtgcttcag tgtggagaca ttactggata tactcatctg cctttatgat gaatgcaata
     1441 attctccatt gagaagagag aagaacattc tcgaatacct agaatgggct aaaccattta
     1501 cttctaaagt gaaacaaatg cgattacata gagaagactt tgaaatatta aaggtgattg
     1561 gtcgaggagc ttttggggag gttgctgtag taaaactaaa aaatgcagat aaagtgtttg
     1621 ccatgaaaat attgaataaa tgggaaatgc tgaaaagagc tgagacagca tgttttcgtg
     1681 aagaaaggga tgtattagtg aatggagaca ataaatggat tacaaccttg cactatgctt
     1741 tccaggatga caataactta tacctggtta tggattatta tgttggtggg gatttgctta
     1801 ctctactcag caaatttgaa gatagattgc ctgaagatat ggctagattt tacttggctg
     1861 agatggtgat agcaattgac tcagttcatc agctacatta tgtacacaga gacattaaac
     1921 ctgacaatat actgatggat atgaatggac atattcggtt agcagatttt ggttcttgtc
     1981 tgaagctgat ggaagatgga acggttcagt cctcagtggc tgtaggaact ccagattata
     2041 tctctcctga aatccttcaa gccatggaag atggaaaagg gagatatgga cctgaatgtg
     2101 actggtggtc tttgggggtc tgtatgtatg aaatgcttta cggagaaaca ccattttatg
     2161 cagaatcgct ggtggagaca tacggaaaaa tcatgaacca caaagagagg tttcagtttc
     2221 cagcccaagt gactgatgtg tctgaaaatg ctaaggatct tattcgaagg ctcatttgta
     2281 gcagagaaca tcgacttggt caaaatggaa tagaagactt taagaaacac ccatttttca
     2341 gtggaattga ttgggataat attcggaact gtgaagcacc ttatattcca gaagttagta
     2401 gcccaacaga tacatcgaat tttgatgtag atgatgattg tttaaaaaat tctgaaacga
     2461 tgcccccacc aacacatact gcattttctg gccaccatct gccatttgtt ggttttacat
     2521 atactagtag ctgtgtactt tctgatcgga gctgtttaag agttacggct ggtcccacct
     2581 cactggatct tgatgttaat gttcagagga ctctagacaa caacttagca actgaagctt
     2641 atgaaagaag aattaagcgc cttgagcaag aaaaacttga actcagtaga aaacttcaag
     2701 agtcaacaca gactgtccaa gctctgcagt attcaactgt tgatggtcca ctaacagcaa
     2761 gcaaagattt agaaataaaa aacttaaaag aagaaattga aaaactaaga aaacaagtaa
     2821 cagaatcaag tcatttggaa cagcaacttg aagaagctaa tgctgtgagg caagaactag
     2881 atgatgcttt tagacaaatc aaggcttatg aaaaacaaat caaaacgtta caacaagaaa
     2941 gagaagatct aaataaggaa ctagtccagg ctagtgagcg attaaaaaac caatccaaag
     3001 agctgaaaga cgcacactgt cagaggaaac tggccatgca ggaattcatg gagatcaatg
     3061 agcggctaac agaattgcac acccaaaaac agaaacttgc tcgccatgtc cgagataagg
     3121 aagaagaggt ggacctggtg atgcaaaaag ttgaaagctt aaggcaagaa ctgcgcagaa
     3181 cagaaagagc caaaaaagag ctggaagttc atacagaagc tctagctgct gaagcatcta
     3241 aagacaggaa gctacgtgaa cagagtgagc actattctaa gcaactggaa aatgaattgg
     3301 agggactgaa gcaaaaacaa attagttact caccaggagt atgcagcata gaacatcagc
     3361 aagagataac caaactaaag actgatttgg aaaagaaaag tatcttttat gaagaagaat
     3421 tatctaaaag agaaggaata catgcaaatg aaataaaaaa tcttaagaaa gaactgcatg
     3481 attcagaagg tcagcaactt gctctcaaca aagaaattat gattttaaaa gacaaattgg
     3541 aaaaaaccag aagagaaagt caaagtgaaa gggaggaatt tgaaagtgag ttcaaacaac
     3601 aatatgaacg agaaaaagtg ttgttaactg aagaaaataa aaagctgacg agtgaacttg
     3661 ataagcttac tactttgtat gagaacttaa gtatacacaa ccagcagtta gaagaagagg
     3721 ttaaagatct agcagacaag aaagaatcag ttgcacattg ggaagcccaa atcacagaaa
     3781 taattcagtg ggtcagcgat gaaaaggatg cacgagggta tcttcaggcc ttagcttcta
     3841 aaatgactga agaattggag gcattaagaa attccagctt gggtacacga gcaacagata
     3901 tgccctggaa aatgcgtcgt tttgcgaaac tggatatgtc agctagactg gagttgcagt
     3961 cggctctgga tgcagaaata agagccaaac aggccatcca agaagagttg aataaagtta
     4021 aagcatctaa tatcataaca gaatgtaaac taaaagattc agagaagaag aacttggaac
     4081 tactctcaga aatcgaacag ctgataaagg acactgaaga gcttagatct gaaaagggta
     4141 tagagcacca agactcacag cattctttct tggcattttt gaatacgcct accgatgctc
     4201 tggatcaatt tgaaactgat cccgttgaga acacatatgt atggaacccg agcgtcaagt
     4261 ttcacatcca gtcacggtcc acatctcctt ccacatctag tgaagctgag ccagttaaga
     4321 ctgtagactc cactccactt tcagttcaca caccaacctt aaggaaaaaa ggatgtcctg
     4381 gttcaactgg ctttccacct aagcgcaaga ctcaccagtt ttttgtaaaa tcttttacta
     4441 ctcctaccaa gtgtcatcag tgtacctcct tgatggtggg tttaataaga cagggctgtt
     4501 catgtgaagt gtgtggattc tcatgccata taacttgtgt aaacaaagct ccaaccactt
     4561 gtccagttcc tcctgaacag acaaaaggtc ccctgggtat agatcctcag aaaggaatag
     4621 gaacagcata tgaaggtcat gtcaggattc ctaagccagc tggagtgaag aaagggtggc
     4681 agagagcact ggctatagtg tgtgacttca aactctttct gtacgatatt gctgaaggaa
     4741 aagcatctca gcccagtgtt gtcattagtc aagtgattga catgagggat gaagaatttt
     4801 ctgtgagttc agtcttggct tctgatgtta tccatgcaag tcggaaagat ataccctgta
     4861 tatttagggt cacagcttcc cagctctcag catctaataa caaatgttca atcctgatgc
     4921 tagcagacac tgagaatgag aagaataagt gggtgggagt gctgagtgaa ttgcacaaga
     4981 ttttgaagaa aaacaaattc agagaccgct cagtctatgt tcccaaagag gcttatgaca
     5041 gcactctacc cctcattaaa acaacccagg cagccgcaat catagatcat gaaagaattg
     5101 ctttgggaaa cgaagaaggg ttatttgttg tacatgtcac caaagatgaa attattagag
     5161 ttggtgacaa taagaagatt catcagattg aactcattcc aaatgatcag cttgttgctg
     5221 tgatctcagg acgaaatcgt catgtacgac tttttcctat gtcagcattg gatgggcgag
     5281 agaccgattt ttacaagctg tcagaaacta aagggtgtca aaccgtaact tctggaaagg
     5341 tgcgccatgg agctctcaca tgcctgtgtg tggctatgaa aaggcaggtc ctctgttatg
     5401 aactatttca gagcaagacc cgtcacagaa aatttaaaga aattcaagtc ccatataatg
     5461 tccagtggat ggcaatcttc agtgaacaac tctgtgtggg attccagtca ggatttctaa
     5521 gatacccctt gaatggagaa ggaaatccat acagtatgct ccattcaaat gaccatacac
     5581 tatcatttat tgcacatcaa ccaatggatg ctatctgcgc agttgagatc tccagtaaag
     5641 aatatctgct gtgttttaac agcattggga tatacactga ctgccagggc cgaagatcta
     5701 gacaacagga attgatgtgg ccagcaaatc cttcctcttg ttgttacaat gcaccatatc
     5761 tctcggtgta cagtgaaaat gcagttgata tctttgatgt gaactccatg gaatggattc
     5821 agactcttcc tctcaaaaag gttcgaccct taaacaatga aggatcatta aatcttttag
     5881 ggttggagac cattagatta atatatttca aaaataagat ggcagaaggg gacgaactgg
     5941 tagtacctga aacatcagat aatagtcgga aacaaatggt tagaaacatt aacaataagc
     6001 ggcgttattc cttcagagtc ccagaagagg aaaggatgca gcagaggagg gaaatgctac
     6061 gagatccaga aatgagaaat aaattaattt ctaatccaac taattttaat cacatagcac
     6121 acatgggtcc tggagatgga atacagatcc tgaaagatct gcccatgaac cctcggcctc
     6181 aggaaagtcg gacagtattc agtggctcag tcagtattcc atctatcacc aaatcccgcc
     6241 ctgagccagg ccgctccatg agtgctagca gtggcttgtc agcaaggtca tccgcacaga
     6301 atggcagcgc attaaagagg gaattctctg gaggaagcta cagtgccaag cggcagccca
     6361 tgccctcccc gtcagagggc tctttgtcct ctggaggcat ggaccaagga agtgatgccc
     6421 cagcgaggga ctttgacgga gaggactctg actctccgag gcattccaca gcttccaaca
     6481 gttccaacct aagcagcccc ccaagcccag cttcaccccg aaaaaccaag agcctctccc
     6541 tggagagcac tgaccgcggg agctgggacc cgtgagctgc ctcagcactg ggacctctcg
     6601 ctctccgctc cctgccactc gcctcctctc actttcatct cttccctcca cctcgcctgc
     6661 tcggcctgaa agccaccagg ggctggcagc agtagcagga cagggattca ggagttctga
     6721 cgacacgact ctcagatcca cgcccccagc ctaacagcaa caacaaagac agactttccg
     6781 tagcagctta gattaacgtt gatttcattc catgcactta gagttgcttt cagtaacatt
     6841 ttacccctac tcccaaaggt agcttaaata gacagattac acaaatgtaa gtgataagaa
     6901 taagattaga cagattttgc tttcacagta gagtctcatt atagtcctaa aatagctcat
     6961 gggcttctcc gcatccagaa gggagaattg gtccctggag tggctcacta agctcttaat
     7021 cagcaaacgc agtgagtatc aacctgattg ttgccaggaa atccttatga attaaaacaa
     7081 tgcatatttt actacagtac agagtttaaa tgaatacata aatgtagaag tactgaatgt
     7141 atatatttaa aaggagcctc ttgtattcaa caaaagatgg atgcatatat aagagagatg
     7201 atttaattta aagaaatatg ttgtttcttg tctgtaatgt aatgtaaagg gtggaaaggc
     7261 ctcaagctca catttgtaga gagagagcga gagaaatcag agttcccttt attgccctgt
     7321 cctcaaactg gtcataggct ctagtcacct ggggagctgt agaaaacact tgcagagcca
     7381 ggttttgctg gtttggggca tgccctgggc accagagctt taacatttga agccacttca
     7441 gcagcagcag caaaaggcga actcatctct acccaagatg tttcttttcc tagtggtgga
     7501 atttgaacac ttctcacttt ttattgtatt ttatcttccg cagataaatg tagaaataca
     7561 cgattctgtc acctctgatc ccttccatct gaaaggttac aaggagtgtt gtagcttctg
     7621 aaggtgcaga aaacaatttc taaaaatgct tttattcctg ggctaatcct gtccctccct
     7681 aagtcacagc gaggtgtctg tcccagggct ggagatgctt cccaaggagg agtctgtttt
     7741 gttgagagtg ggcgtgggct tcttcacata agcctgggga aggaagaaaa aacggctttc
     7801 attaccaaat aatgtaaaac ctcaaaagca agggcttcaa cagccttaac caaatattat
     7861 tccccatagc cagtggaaaa tggatgtgac aaccccagtg cgcaggccag agtgagtgag
     7921 cccagcacgg cgctccgact ggcttcctct ctcaggtgct ggattgtggg gttagtggca
     7981 tttccagctg gattcctcct gttgtagttg ccataaggaa atgagatgca gaatcagaag
     8041 gatctatttc tacagaatca tttcaccagt taagcacatg agtagagaaa gagataaaaa
     8101 taaaagtatc tcatgaagga aagagatttt gcctctcttt tacttttcac ctaagtttct
     8161 ctgagaaata gagacaggat tctctcttta aaattcagtg aaaatgaaga aagttttcct
     8221 gcagttgcta acctgagttg cagtgtttaa ggccatcatt tcactgctgc tgtctgtgac
     8281 tccacgtctg tgtcactgag gtgacctgcg tgtcactgag gtggccacca tgctggcctg
     8341 cggcatgtgc agggagctga ggctgtttcc aggtgatgct gctgtgtgga gaaggttctg
     8401 agatgcagtg agggaagaaa ggatcctgct ggggattcca ttgtaagcac ctataatcgg
     8461 gaattttcat gtaacagctt tgacatttaa acattctgag tttggtgcca gctcagattt
     8521 gattatattt tattttggat gggtgtaatt cacagcacag ttctaatctc ccaaatcttt
     8581 ctgcttttta gaatgaagta taaaaatact tttctcacct gaataccaag ggttggccct
     8641 ttagttggat cattgtcata tgacttggta gatccttgtc ctcagcacct cacgtgagag
     8701 aagggagtca gccagccggc cccctgcttg gtgctcgtga ccagctcgca ccccttctgt
     8761 ccacccttct ctcctctcct ccccactctc cccaccctcc tcactctccc caccctcctc
     8821 actctcccca ccctcccctc ctctcctcct cactcttccc accctcccca tccccaccct
     8881 ccccatcctc ctcttccctt tccccttgcc ttctcctctc tcccttctct tctcaggcag
     8941 ggaggaggcc atcccaagcc gagattaaca ggacttgaca taagccatta gtttgtagct
     9001 ttgacaagta attatgaatt tttgttgctt ataggtgctt attttgcaaa ggatgctttt
     9061 aagatcaaaa taataaccct acctaaagtc tagctccact gctatgggtc atactcttca
     9121 gcctcccaac agggcagaga gagagagcta ctgaggcttg tctaggttgc caggctaact
     9181 gggcgacttg tccatattca ccccatggat tgcaccatgg cactctttga tttttccact
     9241 gcaatggcaa gtaatctcat cagtcataat agagcagtcc cgaatgcgtg cagattctaa
     9301 aagcagggct ttaggagaga aacactgcca gggggaatag ttttggggag ggttttccca
     9361 aaataacggt catcctactg ggtttatccc acccttaaat atgaagcctg ttacctccag
     9421 aagcttctga gaagaatgat gtgaaaagac agggagtggg ttctaggcaa agaaaacata
     9481 atgaccattc agaggagtca gtagcacagc tcacagataa agtattttat tactatctga
     9541 agttttcttt tgttttcatg caggacattt taaaaacgta tatggcagca gaaacctgtt
     9601 tctcaataga aaaaatacat tcagaggcat ttctgggata gtctatctgt gttagtattt
     9661 ggtgctatct atgtccagcc aagttatcta ccctcaaatt ctgactaatc atgtttgtgc
     9721 tttgggtatt taaaattaca tacatatata ttctttttgc caaaaacaaa agtcttgctt
     9781 cttgtcaaat gattgctaaa gtagatctta cattttttgt tattatgtat gtatttatac
     9841 acatccccaa cacacttagt gatttctgtt atttcctagg gagcacagct ttaaggctat
     9901 gagatacaac taaaaggagc ccatctattt ggttttccag ccaattattg tactcacatt
     9961 tcaggggaga atctgaaatt cctgtcatgt ttacagcaac aatctatcat tcctggctag
    10021 ctctcagcct ctctctcctt ccataggtta gaattatgtc attttgttac ttagtggcca
    10081 cgtctatttc tgagaaagac tggttacatt tatgtggcat ctcaggtatc attaaggaaa
    10141 agccagagca ggggtgagca gaggtcaaaa ccacagacgc agcagggcca tttgccgcct
    10201 ttggccggga tcacaaccac tgcagtctcc cagcaggtag gccttgccaa gcctaaggct
    10261 ccccatccaa tctagacaga ggggcgctca gagcagactt tgccgtagcc catgtctggt
    10321 gagcacaaca gggaatgaat tgggcactcc actcccccgt ctctctggcc cagccctgaa
    10381 ctagatgagc tgcatttcat ggagcccatt ttaaaatctc tttccttatg actttgttac
    10441 tcaagtccag agttctctgt gcacttctgc tagataagga gtgtaagccc tgccccccag
    10501 cactggcagc acgctgggcc ctccccacac aggacaccgt gcagttccgg gggaagctga
    10561 ctcaaatcaa ccttgaaatc tcatgaaaac aaaatgactt gtctttttat ttgatagtgt
    10621 aatatcattc attttataaa ttttttaggg tttttctcgt aatattgtac agttttgcat
    10681 ggcctggtgt gatcattttt tggttagaat ataatgctga caaatgtgga tggaggggaa
    10741 gatactgctt tagcctatca ctccttattt tattttgttt ggttttatgc cctcagtgtc
    10801 ttagggaact ttttaagaga tcctctgcta ccaaacaatg atgtggattc ttttgcacag
    10861 aaatatttaa ggtgggatgg taaaaaatgt cacaaaagac tcctcaccaa tactttatgt
    10921 tgatatcact taatattaac cagactttgc tgtattgcaa taaaacagag aactgtta
//
