LOCUS       NM_004359               1418 bp    mRNA    linear   PRI 02-FEB-2025
DEFINITION  Homo sapiens cell division cycle 34, ubiquitin conjugating enzyme
            (CDC34), mRNA.
ACCESSION   NM_004359
VERSION     NM_004359.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Zhang,S. and Sun,Y.
  TITLE     Targeting CDC34 E2 ubiquitin conjugating enzyme for lung cancer
            therapy
  JOURNAL   EBioMedicine 54, 102718 (2020)
   PUBMED   32268269
  REMARK    GeneRIF: Targeting CDC34 E2 ubiquitin conjugating enzyme for lung
            cancer therapy.
REFERENCE   3  (bases 1 to 1418)
  AUTHORS   Zhao,X.C., Wang,G.Z., Wen,Z.S., Zhou,Y.C., Hu,Q., Zhang,B.,
            Qu,L.W., Gao,S.H., Liu,J., Ma,L., Zhang,Y.F., Zhang,C., Yu,H.,
            Zhang,D.L., Wang,M., Wang,C.L., Huang,Y.C., Liu,Z.H., Zhao,Y.,
            Chen,L. and Zhou,G.B.
  TITLE     Systematic identification of CDC34 that functions to stabilize EGFR
            and promote lung carcinogenesis
  JOURNAL   EBioMedicine 53, 102689 (2020)
   PUBMED   32114396
  REMARK    GeneRIF: Systematic identification of CDC34 that functions to
            stabilize EGFR and promote lung carcinogenesis.
REFERENCE   4  (bases 1 to 1418)
  AUTHORS   Hill,S., Reichermeier,K., Scott,D.C., Samentar,L.,
            Coulombe-Huntington,J., Izzi,L., Tang,X., Ibarra,R., Bertomeu,T.,
            Moradian,A., Sweredoski,M.J., Caberoy,N., Schulman,B.A.,
            Sicheri,F., Tyers,M. and Kleiger,G.
  TITLE     Robust cullin-RING ligase function is established by a multiplicity
            of poly-ubiquitylation pathways
  JOURNAL   Elife 8, e51163 (2019)
   PUBMED   31868589
  REMARK    GeneRIF: UBE2R2 alone had negligible ubiquitylation activity at
            physiological concentrations and the ablation of UBE2R1/2 had no
            effect on the stability of SCF substrates in cells. A genome-wide
            CRISPR screen revealed that an additional E2 enzyme, UBE2G1,
            buffers against the loss of UBE2R1/2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1418)
  AUTHORS   Liu,X., Zhang,Y., Hu,Z., Li,Q., Yang,L. and Xu,G.
  TITLE     The Catalytically Inactive Mutation of the Ubiquitin-Conjugating
            Enzyme CDC34 Affects its Stability and Cell Proliferation
  JOURNAL   Protein J 37 (2), 132-143 (2018)
   PUBMED   29564676
  REMARK    GeneRIF: study finds that mutation of the catalytically active
            cysteine to serine (C93S) results in the reduced ubiquitination,
            increased stability, and attenuated degradation rate of CDC34;
            Through semi-quantitative proteomics, identified the
            CDC34-interacting proteins and discovered that the wild-type and
            mutant proteins have many differentially interacted proteins
REFERENCE   6  (bases 1 to 1418)
  AUTHORS   Song,A., Wang,Q., Goebl,M.G. and Harrington,M.A.
  TITLE     Phosphorylation of nuclear MyoD is required for its rapid
            degradation
  JOURNAL   Mol Cell Biol 18 (9), 4994-4999 (1998)
   PUBMED   9710583
REFERENCE   7  (bases 1 to 1418)
  AUTHORS   Lisztwan,J., Marti,A., Sutterluty,H., Gstaiger,M., Wirbelauer,C.
            and Krek,W.
  TITLE     Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34
            with the F-box protein p45(SKP2): evidence for evolutionary
            conservation in the subunit composition of the CDC34-SCF pathway
  JOURNAL   EMBO J 17 (2), 368-383 (1998)
   PUBMED   9430629
REFERENCE   8  (bases 1 to 1418)
  AUTHORS   Pagano,M.
  TITLE     Cell cycle regulation by the ubiquitin pathway
  JOURNAL   FASEB J 11 (13), 1067-1075 (1997)
   PUBMED   9367342
  REMARK    Review article
REFERENCE   9  (bases 1 to 1418)
  AUTHORS   King,R.W., Deshaies,R.J., Peters,J.M. and Kirschner,M.W.
  TITLE     How proteolysis drives the cell cycle
  JOURNAL   Science 274 (5293), 1652-1659 (1996)
   PUBMED   8939846
  REMARK    Review article
REFERENCE   10 (bases 1 to 1418)
  AUTHORS   Plon,S.E., Leppig,K.A., Do,H.N. and Groudine,M.
  TITLE     Cloning of the human homolog of the CDC34 cell cycle gene by
            complementation in yeast
  JOURNAL   Proc Natl Acad Sci U S A 90 (22), 10484-10488 (1993)
   PUBMED   8248134
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011531.7 and AC011556.9.
            
            On Nov 25, 2018 this sequence version replaced NM_004359.1.
            
            Summary: The protein encoded by this gene is a member of the
            ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme
            catalyzes the covalent attachment of ubiquitin to other proteins.
            This protein is a part of the large multiprotein complex, which is
            required for ubiquitin-mediated degradation of cell cycle G1
            regulators, and for the initiation of DNA replication. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC009850.2, SRR1163655.656271.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000215574.9/ ENSP00000215574.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-349               AC011531.7         21249-21597
            350-436             AC011531.7         25326-25412
            437-534             AC011531.7         25732-25829
            535-669             AC011531.7         26502-26636
            670-1418            AC011556.9         9821-10569
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1418
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /note="cell division cycle 34, ubiquitin conjugating
                     enzyme"
                     /db_xref="GeneID:997"
                     /db_xref="HGNC:HGNC:1734"
                     /db_xref="MIM:116948"
     exon            1..349
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..883
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /EC_number="2.3.2.23"
                     /EC_number="2.3.2.24"
                     /note="ubiquitin-conjugating enzyme E2-32 KDA
                     complementing; ubiquitin carrier protein;
                     ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme
                     E2-CDC34; cell division cycle 34 homolog; E2
                     ubiquitin-conjugating enzyme R1; (E3-independent) E2
                     ubiquitin-conjugating enzyme R1; cell division cycle 34,
                     ubiqiutin conjugating enzyme"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 R1"
                     /protein_id="NP_004350.1"
                     /db_xref="CCDS:CCDS12030.1"
                     /db_xref="GeneID:997"
                     /db_xref="HGNC:HGNC:1734"
                     /db_xref="MIM:116948"
                     /translation="MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVA
                     IFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVD
                     DPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDRE
                     YTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEE
                     EADSCFGDDEDDSGTEES"
     exon            350..436
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /inference="alignment:Splign:2.1.0"
     exon            437..534
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /inference="alignment:Splign:2.1.0"
     exon            535..669
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /inference="alignment:Splign:2.1.0"
     exon            670..1418
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1399..1404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /note="hexamer: AATAAA"
     polyA_site      1418
                     /gene="CDC34"
                     /gene_synonym="E2-CDC34; UBC3; UBCH3; UBE2R1"
                     /note="major polyA site"
ORIGIN      
        1 agagctgctg gagcgctcgg ggtccccggg cggcggcggc ggcgcagagg aggaggcagg
       61 cggcggcccc ggtggctccc ccccggacgg tgcgcggccc ggcccgtctc gcgaactcgc
      121 ggtggtcgcg cggccccgcg ctgctccgac cccgggcccc tccgccgccg ccatggctcg
      181 gccgctagtg cccagctcgc agaaggcgct gctgctggag ctcaaggggc tgcaggaaga
      241 gccggtcgag ggattccgcg tgacactggt ggacgagggc gatctataca actgggaggt
      301 ggccatcttc gggcccccca acacctacta cgagggcggc tacttcaagg cgcgcctcaa
      361 gttccccatc gactacccat actctccacc agcctttcgg ttcctgacca agatgtggca
      421 ccctaacatc tacgagacgg gggacgtgtg tatctccatc ctccacccgc cggtggacga
      481 cccccagagc ggggagctgc cctcagagag gtggaacccc acgcagaacg tcaggaccat
      541 tctcctgagt gtgatctccc tcctgaacga gcccaacacc ttctcgcccg caaacgtgga
      601 cgcctccgtg atgtacagga agtggaaaga gagcaagggg aaggatcggg agtacacaga
      661 catcatccgg aagcaggtcc tggggaccaa ggtggacgcg gagcgtgacg gcgtgaaggt
      721 gcccaccacg ctggccgagt actgcgtgaa gaccaaggcg ccggcgcccg acgagggctc
      781 agacctcttc tacgacgact actacgagga cggcgaggtg gaggaggagg ccgacagctg
      841 cttcggggac gatgaggatg actctggcac ggaggagtcc tgacaccacc agaataaact
      901 tgccgagttt acctcactag ggccggaccc gtggctcctt agacgacaga ctacctcacg
      961 gaggttttgt gctggtcccc gtctcctctg gttgtttcgt tttggctttt tctccctccc
     1021 catgtctgtt ctgggttttc acgtgcttca gagaagaggg gctgccccac cgccactcac
     1081 gtcactcggg gctcggtgga cgggcccagg gtgggagcgg ccggcccacc tgtcccctcg
     1141 ggaggggagc tgagcccgac ttctaccggg gtcccccagc ttccggactg gccgcacccc
     1201 ggaggagcca cgggggcgct gctgggaacg tgggcggggg gccgtttcct gacactacca
     1261 gcctgggagg cccaggtgta gcggtccgag gggcccggtc ctgcctgtca gctccaggtc
     1321 ctggagccac gtccagcaca gagtggacgg attcaccgtg gccgactctt ttccctgctt
     1381 tggtttgttt gaaatctaaa taaaactact ttatgaga
//
