LOCUS       NM_001256               5830 bp    mRNA    linear   PRI 28-APR-2025
DEFINITION  Homo sapiens cell division cycle 27 (CDC27), transcript variant 2,
            mRNA.
ACCESSION   NM_001256
VERSION     NM_001256.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Song,W., Xia,X., Fan,Y., Zhang,B. and Chen,X.
  TITLE     Functional and Genetic Analyses Unveil the Implication of CDC27 in
            Hemifacial Microsomia
  JOURNAL   Int J Mol Sci 25 (9), 4707 (2024)
   PUBMED   38731925
  REMARK    GeneRIF: Functional and Genetic Analyses Unveil the Implication of
            CDC27 in Hemifacial Microsomia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Zhao,C., Sun,J., Dang,Z., Su,Q. and Yang,J.
  TITLE     Circ_0000775 promotes the migration, invasion and EMT of hepatic
            carcinoma cells by recruiting IGF2BP2 to stabilize CDC27
  JOURNAL   Pathol Res Pract 235, 153908 (2022)
   PUBMED   35561648
  REMARK    GeneRIF: Circ_0000775 promotes the migration, invasion and EMT of
            hepatic carcinoma cells by recruiting IGF2BP2 to stabilize CDC27.
REFERENCE   3  (bases 1 to 5830)
  AUTHORS   Shang,S., Zhou,Y., Chen,K., Chen,L., Li,P., Li,D., Cui,S.,
            Zhang,M.J., Chen,X. and Li,Q.
  TITLE     A Novel Gene CDC27 Causes SLE and Is Associated With the Disease
            Activity
  JOURNAL   Front Immunol 13, 876963 (2022)
   PUBMED   35418986
  REMARK    GeneRIF: A Novel Gene CDC27 Causes SLE and Is Associated With the
            Disease Activity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5830)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5830)
  AUTHORS   Yu,H., Peters,J.M., King,R.W., Page,A.M., Hieter,P. and
            Kirschner,M.W.
  TITLE     Identification of a cullin homology region in a subunit of the
            anaphase-promoting complex
  JOURNAL   Science 279 (5354), 1219-1222 (1998)
   PUBMED   9469815
REFERENCE   6  (bases 1 to 5830)
  AUTHORS   Ollendorff,V. and Donoghue,D.J.
  TITLE     The serine/threonine phosphatase PP5 interacts with CDC16 and
            CDC27, two tetratricopeptide repeat-containing subunits of the
            anaphase-promoting complex
  JOURNAL   J Biol Chem 272 (51), 32011-32018 (1997)
   PUBMED   9405394
REFERENCE   7  (bases 1 to 5830)
  AUTHORS   Ho,P.P., Couch,F.J., Brody,L.C., Abel,K.J., Boehnke,M.,
            Shearon,T.H., Chandrasekharappa,S.C., Collins,F.S. and Weber,B.L.
  TITLE     Localization of the human homolog of the yeast cell division
            control 27 gene (CDC27) proximal to ITGB3 on human chromosome
            17q21.3
  JOURNAL   Somat Cell Mol Genet 21 (5), 351-355 (1995)
   PUBMED   8619132
REFERENCE   8  (bases 1 to 5830)
  AUTHORS   Tugendreich,S., Tomkiel,J., Earnshaw,W. and Hieter,P.
  TITLE     CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic
            spindle and is essential for the metaphase to anaphase transition
  JOURNAL   Cell 81 (2), 261-268 (1995)
   PUBMED   7736578
REFERENCE   9  (bases 1 to 5830)
  AUTHORS   Chen,P.L., Ueng,Y.C., Durfee,T., Chen,K.C., Yang-Feng,T. and
            Lee,W.H.
  TITLE     Identification of a human homologue of yeast nuc2 which interacts
            with the retinoblastoma protein in a specific manner
  JOURNAL   Cell Growth Differ 6 (2), 199-210 (1995)
   PUBMED   7756179
REFERENCE   10 (bases 1 to 5830)
  AUTHORS   Tugendreich,S., Boguski,M.S., Seldin,M.S. and Hieter,P.
  TITLE     Linking yeast genetics to mammalian genomes: identification and
            mapping of the human homolog of CDC27 via the expressed sequence
            tag (EST) data base
  JOURNAL   Proc Natl Acad Sci U S A 90 (21), 10031-10035 (1993)
   PUBMED   8234252
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY023336.1, S78234.1,
            AC002558.2 and AA927469.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001256.5.
            
            Summary: The protein encoded by this gene shares strong similarity
            with Saccharomyces cerevisiae protein Cdc27, and the gene product
            of Schizosaccharomyces pombe nuc 2. This protein is a component of
            the anaphase-promoting complex (APC), which is composed of eight
            protein subunits and is highly conserved in eukaryotic cells. This
            complex catalyzes the formation of cyclin B-ubiquitin conjugate,
            which is responsible for the ubiquitin-mediated proteolysis of
            B-type cyclins. The protein encoded by this gene and three other
            members of the APC complex contain tetratricopeptide (TPR) repeats,
            which are important for protein-protein interactions. This protein
            was shown to interact with mitotic checkpoint proteins including
            Mad2, p55CDC and BUBR1, and it may thus be involved in controlling
            the timing of mitosis. Alternative splicing of this gene results in
            multiple transcript variants. Related pseudogenes have been
            identified on chromosomes 2, 22 and Y. [provided by RefSeq, May
            2014].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the central coding region, compared to variant 1,
            resulting in an isoform (2) that is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853558.10627.1,
                                           SRR14038193.4312364.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000066544.8/ ENSP00000066544.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                HY023336.1         19-70
            53-2892             S78234.1           30-2869
            2893-5798           AC002558.2         78261-81166
            5799-5830           AA927469.1         11-42               c
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     gene            1..5830
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="cell division cycle 27"
                     /db_xref="GeneID:996"
                     /db_xref="HGNC:HGNC:1728"
                     /db_xref="MIM:116946"
     exon            1..150
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..2598
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     anaphase promoting complex subunit 3; nuc2 homolog;
                     anaphase-promoting complex, protein 3; cell division cycle
                     27 homolog; D0S1430E, D17S978E"
                     /codon_start=1
                     /product="cell division cycle protein 27 homolog isoform
                     2"
                     /protein_id="NP_001247.3"
                     /db_xref="CCDS:CCDS11509.1"
                     /db_xref="GeneID:996"
                     /db_xref="HGNC:HGNC:1728"
                     /db_xref="MIM:116946"
                     /translation="MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLL
                     ATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKS
                     HDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGE
                     KPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLE
                     SSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALS
                     PLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFS
                     QSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKEN
                     SKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQ
                     AFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRA
                     YFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNS
                     PEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACF
                     RNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHAL
                     KKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVY
                     FLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIM
                     GTDESQESSMTDADDTQLHAAESDEF"
     exon            151..226
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            227..374
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            375..500
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            501..598
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            599..753
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            754..965
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1080
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1193
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1293
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1501
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1674
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1827
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..2036
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2154
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2283
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2358
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2515
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..5830
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3072..3077
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="hexamer: AATAAA"
     polyA_site      3098
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="major polyA site"
     regulatory      5309..5314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="hexamer: ATTAAA"
     polyA_site      5336
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
     regulatory      5566..5571
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="hexamer: ATTAAA"
     polyA_site      5589
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="major polyA site"
     regulatory      5808..5813
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
                     /note="hexamer: AATAAA"
     polyA_site      5830
                     /gene="CDC27"
                     /gene_synonym="ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E;
                     H-NUC; HNUC; NUC2"
ORIGIN      
        1 gctcggcctc ccccatcccc cggtaacggt cgctggtgag tttaaatgag caggggctgg
       61 ccgggccgga gccgctacag ggggggcctg aggcactgca gaaagtgggc ctgagcctcg
      121 aggatgacgg tgctgcagga acccgtccag gctgctatat ggcaagcact aaaccactat
      181 gcttaccgag atgcggtttt cctcgcagaa cgcctttatg cagaagtaca ctcagaagaa
      241 gccttgtttt tactggcaac ctgttattac cgctcaggaa aggcatataa agcatataga
      301 ctcttgaaag gacacagttg tactacaccg caatgcaaat acctgcttgc aaaatgttgt
      361 gttgatctca gcaagcttgc agaaggggaa caaatcttat ctggtggagt gtttaataag
      421 cagaaaagcc atgatgatat tgttactgag tttggtgatt cagcttgctt tactctttca
      481 ttgttgggac atgtatattg caagacagat cggcttgcca aaggatcaga atgttaccaa
      541 aagagcctta gtttaaatcc tttcctctgg tctccctttg aatcattatg tgaaataggt
      601 gaaaagccag atcctgacca aacatttaaa ttcacatctt tacagaactt tagcaactgt
      661 ctgcccaact cttgcacaac acaagtacct aatcatagtt tatctcacag acagcctgag
      721 acagttctta cggaaacacc ccaggacaca attgaattaa acagattgaa tttagaatct
      781 tccaattcaa agtactcctt gaatacagat tcctcagtgt cttatattga ttcagctgta
      841 atttcacctg atactgtccc actgggaaca ggaacttcca tattatctaa acaggttcaa
      901 aataaaccaa aaactggtcg aagtttatta ggaggaccag cagctcttag tccattaacc
      961 ccaagttttg ggattttgcc attagaaacc ccaagtcctg gagatggatc ctatttacaa
     1021 aactacacta atacacctcc tgtaattgat gtgccatcca ccggagcccc ttcaaaaaag
     1081 tctgttgcca gaatcggcca aactggaaca aagtctgtct tctcacagag tggaaatagc
     1141 cgagaggtaa ctccaattct tgcacaaaca caaagttctg gtccacaaac aagtacaaca
     1201 cctcaggtat tgagccccac tattacatct cccccaaacg cactgcctcg aagaagttca
     1261 cgactcttta ctagtgacag ctccacaacc aaggagaata gcaaaaaatt aaaaatgaag
     1321 tttccaccta aaatcccaaa cagaaaaaca aaaagtaaaa ctaataaagg aggaataact
     1381 caacctaaca taaatgatag cctggaaatt acaaaattgg actcttccat catttcagaa
     1441 gggaaaatat ccacaatcac acctcagatt caggccttta atctacaaaa agcagcagca
     1501 gaaggtttga tgagccttct tcgtgaaatg gggaaaggtt atttagcttt gtgttcatac
     1561 aactgcaaag aagctataaa tattttgagc catctacctt ctcaccacta caatactggt
     1621 tgggtactgt gccaaattgg aagggcctat tttgaacttt cagagtacat gcaagctgaa
     1681 agaatattct cagaggttag aaggattgag aattatagag ttgaaggcat ggagatctac
     1741 tctacaacac tttggcatct tcaaaaagat gttgctcttt cagttctgtc aaaagactta
     1801 acagacatgg ataaaaattc gccagaggcc tggtgtgctg cagggaactg tttcagtctg
     1861 caacgggaac atgatattgc aattaaattc ttccagagag ctatccaagt tgatccaaat
     1921 tacgcttatg cctatactct attagggcat gagtttgtct taactgaaga attggacaaa
     1981 gcattagctt gttttcgaaa tgctatcaga gtcaatccta gacattataa tgcatggtat
     2041 ggtttaggaa tgatttatta caagcaagaa aaattcagcc ttgcagaaat gcatttccaa
     2101 aaagcgcttg atatcaaccc tcaaagttca gttttacttt gccacattgg agtagttcaa
     2161 catgcactga aaaaatcaga gaaggctttg gataccctaa acaaagccat tgtcattgat
     2221 cccaagaacc ctctatgcaa atttcacaga gcctcagttt tatttgcaaa tgaaaaatat
     2281 aagtctgctt tacaagaact tgaagaattg aaacaaattg ttcccaaaga atccctcgtt
     2341 tacttcttaa taggaaaggt ttacaagaag ttaggtcaaa cgcacctcgc cctgatgaat
     2401 ttctcttggg ctatggattt agatcctaaa ggagccaata accagattaa agaggcaatt
     2461 gataagcgtt atcttccaga tgatgaggag ccaataaccc aagaagaaca gatcatggga
     2521 acagatgaat cccaggagag cagcatgaca gatgcggatg acacacaact tcatgcagct
     2581 gaaagtgatg aattttaact tctggaaatc agacttttac aactggatgt gtgactagtg
     2641 ctgacgtgtt tcttgtccct ctgtatactg agtctttact cttgagctgg cggtgtcatc
     2701 gtccgtcact tataccatga gtgtgccact ttcattggac cctgactgta tacagaatga
     2761 aaggcagtgc aatatttagc tgctaacaag actggctctt ttaccagtat gaatgacaat
     2821 ttatgggggg tagggtgggg aactttcttt tctgtttttc tttaatctcc ctttgttgga
     2881 aagtatcatg aaaggaagag ttatgcttta tcttgaagga accattagat atggaaaata
     2941 gtgatgaacc agagtttctt ggttgctttt tcaaaaattt gtttttattt ggttctgttc
     3001 ctgataaaca gagtaactga ccttcatttc taggttcttc aagaatggtg tttgcaagtg
     3061 ccagatggaa caataaaaga cgttgcctat aacagtgact tgattgccaa ggaatgtaaa
     3121 ttaccttaaa cttgcagtat ctcccataaa caaatgtaat gggcatattg ggactcgtat
     3181 gtaggaatca aatatccctc catacagtgt actcttattc ttggcaagaa tgctttaatg
     3241 tctaaccaag aattttaatt tattcatctt gcttcaaagt gttgatcatt gttgtcttgg
     3301 tattgcaaac tttaaaattg tttcttacca tattgcctct tctctttgag ctctgtggga
     3361 tcacctttaa cattcagaga tgatagagtg gttccccact gagaagccaa aacaaggccc
     3421 ttaataaccc cttaagttca ccatatgaat cagaaggaga acactaaagt ggagagactt
     3481 ttaagagatc atgatagtga aagccttaat ataatcagaa ttgtaccata accttgaatc
     3541 tatatttgtt caaaacatcc ctttgacttt tctaagtgtt tgcttaagca gagtgtaaga
     3601 atgtgtggtt acctttggtt gagatccttt ccattctttt tgactctctg cttcagattt
     3661 tttcagtagt gtgagtcacc aaaacattta ctaagagtaa ttgggtttag gatgttggaa
     3721 atttttagct tgggggaaaa aacattctta tgaaggagat aggttctctt ctgagtttgt
     3781 cataatatag attggtgtct ttggaaaatg gccacaattt taagaattca attatgcata
     3841 taaaatgata attattggaa ttccacagta acagatttaa acagtcttaa attgtttatc
     3901 tcctttactg taatgtattg aaatttttag agaaatttta gttgttaaca ttttattaag
     3961 tgccagtgtc agaatataac aaattatagt ttcttatgaa tgacaggcct acagttatta
     4021 ttctggatta tttgatggag gacaaactta cctgtatttg ttagtcaagc tgtgaaaata
     4081 aggtggatta caaaagatgt gaaaaaaatt ttagtctgta gactcagtaa ttttctataa
     4141 tttactgtta atctcatttg aacatggatt aggtacaatt tataaattaa ttcaagtcag
     4201 ggtctttagg tatcaggtgc cagagagata tttaacagat ttccctacct aaatttatgt
     4261 atatgtactg tctaaaacaa tactttttta aaaaaaagga acagttggga gaaaataaat
     4321 ataatgaaaa attcccagag gctagcactt ggattctaac acgtatgcta ttgtattatc
     4381 cattagttct gtaatattta attttagatt cttttatttt tttaattggc aaagcacaag
     4441 gtgctgtata acagtgtcat ttagagtttt atagaaagct tcaacctgag ttctgcgtta
     4501 taaagcctgg agaaagctaa gcttagaaca taacttgctg aagtataatt atctttttgt
     4561 agcaggaatt tatgtgccag aggtgagagt ctttctggta ctgatttttt gagaccaagg
     4621 ataaaaggat cgttttgtaa gacatgccat ggcaatggct ggttggggga cagtttccgc
     4681 ccaagcttgg cctattttat ttttcctcat acctactttc aaagtcattt aggtatttga
     4741 agccttattt cccacgtagt aacactttct ggcttttgca gtttcttttt ttgtttggtt
     4801 ttgttttttg catggaatgg ggatcaaaca acccgaagaa gaacacattt tgatcaagca
     4861 aaatgtttgc ttcaaatttc agaagtttat tttacagaaa ttaaattaag tagtttgaca
     4921 tccttttctc tgtttcacac atatattagg ttggtgcata agtaattgtg gtttttgcca
     4981 tgacttttat ggcaaaacct gcaattactt ttgcaccaac ttaatacatc tatatacata
     5041 tatatatacg cgcacacact tgttcagaag ttatgttgtg gccttggatt tgtttttccc
     5101 cttggaaatg gttcttaact ctgggatttt agaaggttag aatatttttt caagagaaca
     5161 gtggtactca aaagaatgaa aggtggtccc tacattttct gtattcatca cttaaaattt
     5221 ttaatttttc cgagaactac aagtaacatt tgaaccatgc tgctgttgta ccttaaacaa
     5281 aaactcagtg ataaccagta tttagtctat taaaaatgct ctttttgaag aaaaaagttt
     5341 ggaagtctct gattagccag agtatagtat gagtcttcac tgagaaatat ggtgacccat
     5401 tttctttgtg aaaacctggg aaaatgcaag tgtgggtatg aagtgtgtgt tctgtttgca
     5461 ttgaaacaat gtaattttgt gtcttctttt tgctttaact gacttatttc agaaattgta
     5521 cagtgttgag ggggaaagtc ttttctgtta atatatttgc aattcattaa aggatatgga
     5581 aaatccaaat aagttacttt tgaaagcaat ttataatcaa gttgttaaag ctatatgaaa
     5641 gctgtgccat ctgtatttta ataagaaggc acagaaaaac agagatacag ttttatgttt
     5701 cacaaaaaac tagagcaatg tttatctaat tttttaaaac ttacactctt aatatattta
     5761 tatttttaaa cggtcatact cttttttagt attatttgga atatcaaaat aaatactatg
     5821 acaaaaacaa
//
