LOCUS       NM_004931               4794 bp    mRNA    linear   PRI 06-JAN-2023
DEFINITION  Homo sapiens CD8 subunit beta (CD8B), transcript variant 5, mRNA.
ACCESSION   NM_004931
VERSION     NM_004931.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Houser MC, Caudle WM, Chang J, Kannarkat GT, Yang Y, Kelly SD,
            Oliver D, Joers V, Shannon KM, Keshavarzian A and Tansey MG.
  TITLE     Experimental colitis promotes sustained, sex-dependent,
            T-cell-associated neuroinflammation and parkinsonian neuropathology
  JOURNAL   Acta Neuropathol Commun 9 (1), 139 (2021)
   PUBMED   34412704
  REMARK    GeneRIF: Experimental colitis promotes sustained, sex-dependent,
            T-cell-associated neuroinflammation and parkinsonian
            neuropathology.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Esgalhado AJ, Reste-Ferreira D, Albino SE, Sousa A, Amaral AP,
            Martinho A, Oliveira IT, Verde I, Lourenco O, Fonseca AM, Cardoso
            EM and Arosa FA.
  TITLE     CD45RA, CD8beta, and IFNgamma Are Potential Immune Biomarkers of
            Human Cognitive Function
  JOURNAL   Front Immunol 11, 592656 (2020)
   PUBMED   33324408
  REMARK    GeneRIF: CD45RA, CD8beta, and IFNgamma Are Potential Immune
            Biomarkers of Human Cognitive Function.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4794)
  AUTHORS   Liu D, Hu Y, Guo Y, Zhu Z, Lu B, Wang X and Huang Y.
  TITLE     Mycoplasma-associated multidrug resistance of hepatocarcinoma cells
            requires the interaction of P37 and Annexin A2
  JOURNAL   PLoS One 12 (10), e0184578 (2017)
   PUBMED   28976984
  REMARK    GeneRIF: results suggest that mycoplasma induces a resistance to
            multiple drugs in hepatocarcinoma cells which required the
            interaction of P37 and Annexin A2. The pathway downstream this
            interaction needs to be explored
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4794)
  AUTHORS   Pascual-Garcia M, Bertolo C, Nieto JC, Serrat N, Espinosa I,
            D'Angelo E, Munoz R, Rovira R, Vidal S and Prat J.
  TITLE     CD8 down-regulation on cytotoxic T lymphocytes of patients with
            endometrioid endometrial carcinomas
  JOURNAL   Hum Pathol 56, 180-188 (2016)
   PUBMED   27346574
  REMARK    GeneRIF: Endometrioid endometrial carcinomas are capable of
            down-regulating CD8 expression by cytotoxic T lymphocytes.
REFERENCE   5  (bases 1 to 4794)
  AUTHORS   Thakral D, Dobbins J, Devine L and Kavathas PB.
  TITLE     Differential expression of the human CD8beta splice variants and
            regulation of the M-2 isoform by ubiquitination
  JOURNAL   J Immunol 180 (11), 7431-7442 (2008)
   PUBMED   18490743
  REMARK    GeneRIF: study demonstrated differential mRNA expression patterns
            of CD8beta splice variants in thymocytes and in resting, memory,
            and activated primary CD8(+) T cells
REFERENCE   6  (bases 1 to 4794)
  AUTHORS   Nakayama K, Kawachi Y, Tokito S, Minami N, Yamamoto R, Imai T,
            Gachelin G and Nakauchi H.
  TITLE     Recent duplication of the two human CD8 beta-chain genes
  JOURNAL   J Immunol 148 (6), 1919-1927 (1992)
   PUBMED   1541829
REFERENCE   7  (bases 1 to 4794)
  AUTHORS   Terry LA, DiSanto JP, Small TN and Flomenberg N.
  TITLE     Differential expression and regulation of the human CD8 alpha and
            CD8 beta chains
  JOURNAL   Tissue Antigens 35 (2), 82-91 (1990)
   PUBMED   2111591
REFERENCE   8  (bases 1 to 4794)
  AUTHORS   Parnes JR.
  TITLE     Molecular biology and function of CD4 and CD8
  JOURNAL   Adv Immunol 44, 265-311 (1989)
   PUBMED   2493728
  REMARK    Review article
REFERENCE   9  (bases 1 to 4794)
  AUTHORS   Norment AM and Littman DR.
  TITLE     A second subunit of CD8 is expressed in human T cells
  JOURNAL   EMBO J 7 (11), 3433-3439 (1988)
   PUBMED   3145195
REFERENCE   10 (bases 1 to 4794)
  AUTHORS   Johnson,P.
  TITLE     A human homolog of the mouse CD8 molecule, Lyt-3: genomic sequence
            and expression
  JOURNAL   Immunogenetics 26 (3), 174-177 (1987)
   PUBMED   3114136
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC405359.1, BC100912.2,
            X13444.1, AC111200.7, DB223179.1 and BP392114.1.
            
            On May 17, 2019 this sequence version replaced NM_004931.4.
            
            Summary: The CD8 antigen is a cell surface glycoprotein found on
            most cytotoxic T lymphocytes that mediates efficient cell-cell
            interactions within the immune system. The CD8 antigen, acting as a
            coreceptor, and the T-cell receptor on the T lymphocyte recognize
            antigens displayed by an antigen presenting cell (APC) in the
            context of class I MHC molecules. The functional coreceptor is
            either a homodimer composed of two alpha chains, or a heterodimer
            composed of one alpha and one beta chain. Both alpha and beta
            chains share significant homology to immunoglobulin variable light
            chains. This gene encodes the CD8 beta chain isoforms. Multiple
            alternatively spliced transcript variants encoding distinct
            membrane associated or secreted isoforms have been described. A
            pseudogene, also located on chromosome 2, has been identified.
            [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (5), also known as M-1, differs in
            the 3' coding region and 3' UTR, compared to variant 1. The
            resulting isoform (2) has a distinct C-terminus and is shorter than
            isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.205552.1,
                                           SRR7410570.205797.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467150 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000390655.12/ ENSP00000375070.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                DC405359.1         39-83
            46-46               BC100912.2         75-75
            47-652              X13444.1           76-681
            653-848             AC111200.7         8227-8422           c
            849-1301            DB223179.1         1-453
            1302-1302           BP392114.1         427-427
            1303-1395           DB223179.1         455-547
            1396-4794           AC111200.7         4281-7679           c
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..4794
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /note="CD8 subunit beta"
                     /db_xref="GeneID:926"
                     /db_xref="HGNC:HGNC:1707"
                     /db_xref="MIM:186730"
     exon            1..64
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..654
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /note="isoform 5 precursor is encoded by transcript
                     variant 5; CD8 antigen, beta polypeptide 1 (p37); T
                     lymphocyte surface glycoprotein beta chain; Ly-3 homolog;
                     CD8b molecule"
                     /codon_start=1
                     /product="T-cell surface glycoprotein CD8 beta chain
                     isoform 5 precursor"
                     /protein_id="NP_004922.1"
                     /db_xref="CCDS:CCDS1997.1"
                     /db_xref="GeneID:926"
                     /db_xref="HGNC:HGNC:1707"
                     /db_xref="MIM:186730"
                     /translation="MRPRLWLLLAAQLTVLHGNSVLQQTPAYIKVQTNKMVMLSCEAK
                     ISLSNMRIYWLRQRQAPSSDSHHEFLALWDSAKGTIHGEEVEQEKIAVFRDASRFILN
                     LTSVKPEDSGIYFCMIVGSPELTFGKGTQLSVVDFLPTTAQPTKKSTLKKRVCRLPRP
                     ETQKGPLCSPITLGLLVAGVLVLLVSLGVAIHLCCRRRRARLRFMKQFYK"
     exon            65..424
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /inference="alignment:Splign:2.1.0"
     exon            425..514
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /inference="alignment:Splign:2.1.0"
     exon            515..604
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /inference="alignment:Splign:2.1.0"
     exon            605..641
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /inference="alignment:Splign:2.1.0"
     exon            642..4794
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1352..1357
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /note="hexamer: AATAAA"
     polyA_site      1375
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /note="major polyA site"
     regulatory      2257..2262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
                     /note="hexamer: AATAAA"
     polyA_site      2280
                     /gene="CD8B"
                     /gene_synonym="CD8B1; CD8beta; LEU2; Ly-3; LY3; LYT3; P37"
ORIGIN      
        1 aggtgtcccg ggcgcgccac gatgcggccg cggctgtggc tcctcttggc cgcgcagctg
       61 acagttctcc atggcaactc agtcctccag cagacccctg catacataaa ggtgcaaacc
      121 aacaagatgg tgatgctgtc ctgcgaggct aaaatctccc tcagtaacat gcgcatctac
      181 tggctgagac agcgccaggc accgagcagt gacagtcacc acgagttcct ggccctctgg
      241 gattccgcaa aagggactat ccacggtgaa gaggtggaac aggagaagat agctgtgttt
      301 cgggatgcaa gccggttcat tctcaatctc acaagcgtga agccggaaga cagtggcatc
      361 tacttctgca tgatcgtcgg gagccccgag ctgaccttcg ggaagggaac tcagctgagt
      421 gtggttgatt tccttcccac cactgcccag cccaccaaga agtccaccct caagaagaga
      481 gtgtgccggt tacccaggcc agagacccag aagggcccac tttgtagccc catcaccctt
      541 ggcctgctgg tggctggcgt cctggttctg ctggtttccc tgggagtggc catccacctg
      601 tgctgccggc ggaggagagc ccggcttcgt ttcatgaaac aattttacaa atgagcagag
      661 aatacggttt tggtgtcctg ctacaaaaag acatcggtca gtaacgagca cgatgtggaa
      721 aaatgagaga agggacacat tcaaccctgg agagttcaat ggctgctgaa gctgcctgct
      781 tttcactgct gcaaggcctt tctgtgtgtg atgtgcatgg gagcaacttg ttcgtgggtc
      841 atcgggaata ctagggagaa ggtttcattg cccccagggc acttcacaga gtgtgctgga
      901 ggactgagta agaaatgctg cccatgccac cgcttccggc tcctgtgctt tccctgaact
      961 gggaccttta gtggtggcca tttagccacc atctttgcag gttgctttgc cctggtaggg
     1021 cagtaacatt gggtcctggg tctttcatgg ggtgatgctg ggctggctcc ctcttggtct
     1081 tcccaggctg gggctgacct tcctcgcaga gaggccaggt gcaggttggg aatgaggctt
     1141 gctgagaggg gctgtccagt tcccagaagg catatcagtc tctgagggct tcctttgggg
     1201 ccgggaactt gcgggtttga ggataggagt tcacttcatc ttctcagctc ccatttctac
     1261 tcttaagttt ctcagctccc atttctactc tcccatggct taatgcttct ttcattttct
     1321 gtttgtttta tacaaatgtc ttagttgtac aaataaagtc ccaggttaaa gataacaaac
     1381 ggctcctgtg acataaacgt gcgaaagccc atctacagca aagacatcag tgctccgtgg
     1441 aacagaaacc agaaggagaa aatttgtaca tcctcctttt gcacctgaga tcctcatttg
     1501 cccgacgttg taggtgtgga gagtcctaga gaggctagga agcgcccagg gcacccagag
     1561 ccaggctgca ggtgcctcag gcccctctcc cagcccactc ccaagctgaa ctagcacgtg
     1621 ttcatttcta ccgcgggttg aaccgcaggg atccctggct tcaagtcagg caccaaacag
     1681 aaggtaggag gcatgagggg ttctgtcaca tgtctcttca ttttcttatt gattcttagc
     1741 cttgacagtt ggagaaggaa aaagcaagga gaaagtgctg agcaggagtg gagagtgaag
     1801 agcaggacct cgtgcctggg tttgaaaaag tgcctgagac ccatcttagc cgcccctacc
     1861 tgagctttag cagcctagga gctattgcac cataaaacac tgcaacctga ggctgcatta
     1921 acagaagcac cacgtccagg tccagggaga atccagcctc acctgctttt cctggccagc
     1981 tcaaagaagg acattgacaa atcaggttat gttcagttac ctgtgacaac cacagaatgt
     2041 aagaaatgtt acaaaaaaaa caggaaagga aagacgactc tggggaaggg gcagtgtgac
     2101 cttctcctgg catctggggt gcggccgcag cagaatgagg tgtggtgggt ggagttagaa
     2161 aggcatagat gcagtgttac cggaaggagg gccttgagtg taagttgtcc aggtccttgg
     2221 cattttgaac aaagaactga acaaaacata caaagtaata aaggaataaa agcagcaaaa
     2281 ggaagaattt attgaagtga gaaagcactc cacatggtag gagtggaccc taagcagata
     2341 gcccatgggc ccaattgcaa agttttctgg gttttaagta ccccttttga ggtacctgtt
     2401 ggctacccct tatctgggtg aaggatttgg tccatggcta atgaaaggct gaggtgaatt
     2461 gacgccctat gcggatgaag ggatggcccg tgcttggtct gtggtcaatc cagggccgtc
     2521 tccctttcca tctgagatgc agtggaaggg ggagggttgt agggagagta gcctttgatc
     2581 cttggttact cagtgtgggg agatggggtt tttccttttg gtgtatgtta attggcctta
     2641 gatgccctgc ccccagaccc aggtgttttc cgtttgctcc agctttgaga agtcagcaca
     2701 aattggcctc agattccctg cccccagacg taggtgtttc tccttcattc agcacgaatt
     2761 ggccttagat gccctgcccc cagacccagg tgttttccat ttgatccagc tttgagaagt
     2821 cagcacaaat tggcctcaga ttccctgccc ccggacatag atgtttctcc ttgattcagc
     2881 acgaattggc cttagattcc ctgcccccag accctagact cctgcctcag caagaggtgt
     2941 tcaaaagaaa caaaagctct gcccatcagc tctgaggaca agggacaggc tgccttccaa
     3001 ggctgagggg aggggtgaaa gaattggggt cagctggacc agactcctct agggttcgtg
     3061 tttcaggtgg gcccctcacc cggcccatca caaacaaata agagattaag gcctgcagtg
     3121 cacatggtct gccctccttg aaggctcaca cccagtgcac agcggaagta agaacaggcc
     3181 acaaggcctc ttgtcccact gcaggctttg aaatttgacc gtctccctgc tgctgtcttt
     3241 gcaattgcat gtacttgttc acaagttctc atggggttgg gctgggggca ggggtggttt
     3301 gcgctcctct gtcagttttc tgtgcagcag gcacctgccg aggcgggctc agctccggct
     3361 gcccagggag ctggagcagg ctgggctgcc aatggtgggg gctgcggtga gaaggtctgc
     3421 acccagcacc tgacctttgt ttgaagaagg agctgagctg ctactacact ctatagggcc
     3481 attatgatga aatatgcccc ccaaactcca tccagctatc ttttgactag caattccact
     3541 tgttggcatt tatctgaaag aaatacatca aaaagtggca tgcacaaaga aatacgcatc
     3601 agattgttcc ttctagtgtt gcttactagc taaaaatggg aaataatcca aatgtccatc
     3661 aaaagggact ggtcagataa attcagacac agtctaataa gggggtttag gcggctgcta
     3721 aggaatgagc tagatccata tatgctgata tcaaatgatg gttatgacaa atgcatatat
     3781 ttttaaaaca tctgtataga ttattcttaa tacaatatat ggcacaatat gtagtcactg
     3841 ttaaaattca agcacaaaag aaaacaagca aaacaaatat atgttgttag aagttaggat
     3901 acagttaccc ttggtccagt ggtggtcatt aggagggagc atgaggtttg aaggtgagca
     3961 tttctgcttc tcaatctggg aatcacttat atgagtgtgt ttcattagta aaagtcatca
     4021 agctgtacac tcagatatgt tgactttaga gctatggata tagatgtaaa ttatacttca
     4081 agaaagagac tttttaaaat ccaagtacag aacattgtag aaatgtgtaa aattggtatt
     4141 tggaagtttc ttcagcaagc acatgggatt acagctcacg cctgtaatcc cagtactttg
     4201 ggaggcccac acagaatgat cacttgagct caggagtttg agagcagcct aggcaacata
     4261 gcaagacctc gactctacaa aaaattttta aatttttttc tgggcatggt ggtgcttgcc
     4321 tgtagtccca gctattaggg aggatgaggt gggaggatct cctgacccca ggagtttcag
     4381 actgcagtga gctgtgatca cgccactgca ctccagcctg ggcaacacag acagaccttg
     4441 tctcaaaaat aaattttttc taaaaagttt cttgtttttt tttcttccca tattttttaa
     4501 aacataaaat tgaaatcttg agaatataat acataaaata gagaatatag aaaattacat
     4561 cttctaatat tgttctgcag atttgatctt ttttctctaa taataaatca aggcctccgg
     4621 gtatatttgt ttgtatatgt acaggaaaag gtctgtaagg atttactttc agctgttaat
     4681 tgcagttact ctgcggaatt tccacttctg ctttgtacag ttccttgtgt gaatttttac
     4741 atcatgtatt acttttgtag ttggggaaaa agcaattaaa agtttggaaa aaaa
//
