LOCUS       NM_001780               1023 bp    mRNA    linear   PRI 01-JUL-2024
DEFINITION  Homo sapiens CD63 molecule (CD63), transcript variant 1, mRNA.
ACCESSION   NM_001780 NM_001257405
VERSION     NM_001780.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Suzuki,K., Okawa,Y., Akter,S., Ito,H. and Shiba,Y.
  TITLE     Arf GTPase-Activating proteins ADAP1 and ARAP1 regulate
            incorporation of CD63 in multivesicular bodies
  JOURNAL   Biol Open 13 (5) (2024)
   PUBMED   38682696
  REMARK    GeneRIF: Arf GTPase-Activating proteins ADAP1 and ARAP1 regulate
            incorporation of CD63 in multivesicular bodies.
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Dicenta,V., Pelzer,A., Laspa,Z., Castor,T., Gawaz,M.P. and
            Rohlfing,A.K.
  TITLE     The subtilisin-like protease furin regulates hemin-induced CD63
            surface expression on platelets
  JOURNAL   Biochem Biophys Res Commun 701, 149629 (2024)
   PUBMED   38330730
  REMARK    GeneRIF: The subtilisin-like protease furin regulates hemin-induced
            CD63 surface expression on platelets.
REFERENCE   3  (bases 1 to 1023)
  AUTHORS   Fan,Y., Pionneau,C., Cocozza,F., Boelle,P.Y., Chardonnet,S.,
            Charrin,S., Thery,C., Zimmermann,P. and Rubinstein,E.
  TITLE     Differential proteomics argues against a general role for CD9, CD81
            or CD63 in the sorting of proteins into extracellular vesicles
  JOURNAL   J Extracell Vesicles 12 (8), e12352 (2023)
   PUBMED   37525398
  REMARK    GeneRIF: Differential proteomics argues against a general role for
            CD9, CD81 or CD63 in the sorting of proteins into extracellular
            vesicles.
REFERENCE   4  (bases 1 to 1023)
  AUTHORS   Tognoli,M.L., Dancourt,J., Bonsergent,E., Palmulli,R., de
            Jong,O.G., Van Niel,G., Rubinstein,E., Vader,P. and Lavieu,G.
  TITLE     Lack of involvement of CD63 and CD9 tetraspanins in the
            extracellular vesicle content delivery process
  JOURNAL   Commun Biol 6 (1), 532 (2023)
   PUBMED   37198427
  REMARK    GeneRIF: Lack of involvement of CD63 and CD9 tetraspanins in the
            extracellular vesicle content delivery process.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1023)
  AUTHORS   Imai,T., Fukudome,K., Takagi,S., Nagira,M., Furuse,M., Fukuhara,N.,
            Nishimura,M., Hinuma,Y. and Yoshie,O.
  TITLE     C33 antigen recognized by monoclonal antibodies inhibitory to human
            T cell leukemia virus type 1-induced syncytium formation is a
            member of a new family of transmembrane proteins including CD9,
            CD37, CD53, and CD63
  JOURNAL   J Immunol 149 (9), 2879-2886 (1992)
   PUBMED   1401919
REFERENCE   6  (bases 1 to 1023)
  AUTHORS   Hotta,H., Miyamoto,H., Hara,I., Takahashi,N. and Homma,M.
  TITLE     Genomic structure of the ME491/CD63 antigen gene and functional
            analysis of the 5'-flanking regulatory sequences
  JOURNAL   Biochem Biophys Res Commun 185 (1), 436-442 (1992)
   PUBMED   1599482
REFERENCE   7  (bases 1 to 1023)
  AUTHORS   Wang,M.X., Earley,J.J. Jr., Shields,J.A. and Donoso,L.A.
  TITLE     An ocular melanoma-associated antigen. Molecular characterization
  JOURNAL   Arch Ophthalmol 110 (3), 399-404 (1992)
   PUBMED   1339263
REFERENCE   8  (bases 1 to 1023)
  AUTHORS   Fukami,M.H.
  TITLE     Isolation of dense granules from human platelets
  JOURNAL   Methods Enzymol 215, 36-42 (1992)
   PUBMED   1435334
REFERENCE   9  (bases 1 to 1023)
  AUTHORS   Horejsi,V. and Vlcek,C.
  TITLE     Novel structurally distinct family of leucocyte surface
            glycoproteins including CD9, CD37, CD53 and CD63
  JOURNAL   FEBS Lett 288 (1-2), 1-4 (1991)
   PUBMED   1879540
  REMARK    Review article
REFERENCE   10 (bases 1 to 1023)
  AUTHORS   Metzelaar,M.J., Wijngaard,P.L., Peters,P.J., Sixma,J.J.,
            Nieuwenhuis,H.K. and Clevers,H.C.
  TITLE     CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a
            screening procedure for intracellular antigens in eukaryotic cells
  JOURNAL   J Biol Chem 266 (5), 3239-3245 (1991)
   PUBMED   1993697
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from EB386351.1, BU960599.1 and
            AC009779.18.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_001780.5.
            
            Summary: The protein encoded by this gene is a member of the
            transmembrane 4 superfamily, also known as the tetraspanin family.
            Most of these members are cell-surface proteins that are
            characterized by the presence of four hydrophobic domains. The
            proteins mediate signal transduction events that play a role in the
            regulation of cell development, activation, growth and motility.
            The encoded protein is a cell surface glycoprotein that is known to
            complex with integrins. It may function as a blood platelet
            activation marker. Deficiency of this protein is associated with
            Hermansky-Pudlak syndrome. Also this gene has been associated with
            tumor progression. Alternative splicing results in multiple
            transcript variants encoding different protein isoforms. [provided
            by RefSeq, Apr 2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (A). Variants 1, 3-5, and 10-15 all encode the same isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK311893.1, CD050041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257857.9/ ENSP00000257857.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-743               EB386351.1         65-807
            744-903             BU960599.1         249-408
            904-1023            AC009779.18        11756-11875
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..1023
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /note="CD63 molecule"
                     /db_xref="GeneID:967"
                     /db_xref="HGNC:HGNC:1692"
                     /db_xref="MIM:155740"
     exon            1..57
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     exon            58..134
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..785
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /note="isoform A is encoded by transcript variant 1; CD63
                     antigen (melanoma 1 antigen); ocular melanoma-associated
                     antigen; melanoma-associated antigen ME491; limp1;
                     granulophysin; melanoma-associated antigen MLA1;
                     tetraspanin-30; tspan-30; AD1 antigen"
                     /codon_start=1
                     /product="CD63 antigen isoform A"
                     /protein_id="NP_001771.1"
                     /db_xref="CCDS:CCDS8890.1"
                     /db_xref="GeneID:967"
                     /db_xref="HGNC:HGNC:1692"
                     /db_xref="MIM:155740"
                     /translation="MAVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTII
                     QGATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAI
                     AGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSM
                     SKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEV
                     LGIVFACCLVKSIRSGYEVM"
     exon            135..323
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     exon            324..398
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     exon            399..494
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     exon            495..635
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     exon            636..719
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     exon            720..1023
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      847..852
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /note="hexamer: ATTAAA"
     polyA_site      876
                     /gene="CD63"
                     /gene_synonym="AD1; HOP-26; ME491; MLA1; OMA81H; Pltgp40;
                     TSPAN30"
                     /note="major polyA site"
ORIGIN      
        1 agagagcccc ggagccgcgg cgggagagga acgcgcagcc agccttggga agcccaggcc
       61 cggcagccat ggcggtggaa ggaggaatga aatgtgtgaa gttcttgctc tacgtcctcc
      121 tgctggcctt ttgcgcctgt gcagtgggac tgattgccgt gggtgtcggg gcacagcttg
      181 tcctgagtca gaccataatc cagggggcta cccctggctc tctgttgcca gtggtcatca
      241 tcgcagtggg tgtcttcctc ttcctggtgg cttttgtggg ctgctgcggg gcctgcaagg
      301 agaactattg tcttatgatc acgtttgcca tctttctgtc tcttatcatg ttggtggagg
      361 tggccgcagc cattgctggc tatgtgttta gagataaggt gatgtcagag tttaataaca
      421 acttccggca gcagatggag aattacccga aaaacaacca cactgcttcg atcctggaca
      481 ggatgcaggc agattttaag tgctgtgggg ctgctaacta cacagattgg gagaaaatcc
      541 cttccatgtc gaagaaccga gtccccgact cctgctgcat taatgttact gtgggctgtg
      601 ggattaattt caacgagaag gcgatccata aggagggctg tgtggagaag attgggggct
      661 ggctgaggaa aaatgtgctg gtggtagctg cagcagccct tggaattgct tttgtcgagg
      721 ttttgggaat tgtctttgcc tgctgcctcg tgaagagtat cagaagtggc tacgaggtga
      781 tgtaggggtc tggtctcctc agcctcctca tctgggggag tggaatagta tcctccaggt
      841 ttttcaatta aacggattat tttttcagac cgaaaagaga tggtctgagt ttgtcttaga
      901 gtgatgcttg attccttcct ttccttactg atgttccctg tcctctggga ccttaatgca
      961 tgtgtacttc gaggtctatt ttggggggtg tttgggaaag gaggactttg cgaaggtgtt
     1021 ggt
//
