LOCUS       NM_014143               3634 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.
ACCESSION   NM_014143
VERSION     NM_014143.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Murray,C.E., Kornepati,A.V.R., Ontiveros,C., Liao,Y., de la Pena
            Avalos,B., Rogers,C.M., Liu,Z., Deng,Y., Bai,H., Kari,S.,
            Padron,A.S., Boyd,J.T., Reyes,R., Clark,C.A., Svatek,R.S., Li,R.,
            Hu,Y., Wang,M., Conejo-Garcia,J.R., Byers,L.A., Ramkumar,K.,
            Sood,A.K., Lee,J.M., Burd,C.E., Vadlamudi,R.K., Gupta,H.B.,
            Zhao,W., Dray,E., Sung,P. and Curiel,T.J.
  TITLE     Tumour-intrinsic PDL1 signals regulate the Chk2 DNA damage response
            in cancer cells and mediate resistance to Chk1 inhibitors
  JOURNAL   Mol Cancer 23 (1), 242 (2024)
   PUBMED   39478560
  REMARK    GeneRIF: Tumour-intrinsic PDL1 signals regulate the Chk2 DNA damage
            response in cancer cells and mediate resistance to Chk1 inhibitors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Cheng,X., Veverka,V., Radhakrishnan,A., Waters,L.C., Muskett,F.W.,
            Morgan,S.H., Huo,J., Yu,C., Evans,E.J., Leslie,A.J., Griffiths,M.,
            Stubberfield,C., Griffin,R., Henry,A.J., Jansson,A., Ladbury,J.E.,
            Ikemizu,S., Carr,M.D. and Davis,S.J.
  TITLE     Structure and interactions of the human programmed cell death 1
            receptor
  JOURNAL   J Biol Chem 288 (17), 11771-11785 (2013)
   PUBMED   23417675
REFERENCE   3  (bases 1 to 3634)
  AUTHORS   Amarnath,S., Costanzo,C.M., Mariotti,J., Ullman,J.L., Telford,W.G.,
            Kapoor,V., Riley,J.L., Levine,B.L., June,C.H., Fong,T., Warner,N.L.
            and Fowler,D.H.
  TITLE     Regulatory T cells and human myeloid dendritic cells promote
            tolerance via programmed death ligand-1
  JOURNAL   PLoS Biol 8 (2), e1000302 (2010)
   PUBMED   20126379
  REMARK    GeneRIF: Human Tregs can promote immune suppression via DC
            modulation through PD-L1 up-regulation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3634)
  AUTHORS   Lin,D.Y., Tanaka,Y., Iwasaki,M., Gittis,A.G., Su,H.P., Mikami,B.,
            Okazaki,T., Honjo,T., Minato,N. and Garboczi,D.N.
  TITLE     The PD-1/PD-L1 complex resembles the antigen-binding Fv domains of
            antibodies and T cell receptors
  JOURNAL   Proc Natl Acad Sci U S A 105 (8), 3011-3016 (2008)
   PUBMED   18287011
REFERENCE   5  (bases 1 to 3634)
  AUTHORS   Trabattoni,D., Saresella,M., Biasin,M., Boasso,A., Piacentini,L.,
            Ferrante,P., Dong,H., Maserati,R., Shearer,G.M., Chen,L. and
            Clerici,M.
  TITLE     B7-H1 is up-regulated in HIV infection and is a novel surrogate
            marker of disease progression
  JOURNAL   Blood 101 (7), 2514-2520 (2003)
   PUBMED   12468426
  REMARK    GeneRIF: up-regulation in HIV infection, relation to disease
            progression, and possible role in AIDS progression
REFERENCE   6  (bases 1 to 3634)
  AUTHORS   Brown,J.A., Dorfman,D.M., Ma,F.R., Sullivan,E.L., Munoz,O.,
            Wood,C.R., Greenfield,E.A. and Freeman,G.J.
  TITLE     Blockade of programmed death-1 ligands on dendritic cells enhances
            T cell activation and cytokine production
  JOURNAL   J Immunol 170 (3), 1257-1266 (2003)
   PUBMED   12538684
  REMARK    GeneRIF: Blockade of PD-L1 during T cell responses initiated by
            allogenic dendritic cells increases T cell proliferation and
            cytokine production, showing that PD-L1 functions to inhibit T cell
            activation.
REFERENCE   7  (bases 1 to 3634)
  AUTHORS   Mazanet,M.M. and Hughes,C.C.
  TITLE     B7-H1 is expressed by human endothelial cells and suppresses T cell
            cytokine synthesis
  JOURNAL   J Immunol 169 (7), 3581-3588 (2002)
   PUBMED   12244148
  REMARK    GeneRIF: B7-H1 is inducible on human endothelial cells by IFN-gamma
            both in vitro and in vivo; blocking its interaction with its
            receptor (PD-1) on T cells augments T cell cytokine synthesis.
REFERENCE   8  (bases 1 to 3634)
  AUTHORS   Dong,H., Strome,S.E., Salomao,D.R., Tamura,H., Hirano,F.,
            Flies,D.B., Roche,P.C., Lu,J., Zhu,G., Tamada,K., Lennon,V.A.,
            Celis,E. and Chen,L.
  TITLE     Tumor-associated B7-H1 promotes T-cell apoptosis: a potential
            mechanism of immune evasion
  JOURNAL   Nat Med 8 (8), 793-800 (2002)
   PUBMED   12091876
  REMARK    GeneRIF: Cancer cell-associated B7-H1 increases apoptosis of
            antigen-specific human T-cell clones in vitro
            Erratum:[Nat Med 2002 Sep;8(9):1039]
REFERENCE   9  (bases 1 to 3634)
  AUTHORS   Freeman,G.J., Long,A.J., Iwai,Y., Bourque,K., Chernova,T.,
            Nishimura,H., Fitz,L.J., Malenkovich,N., Okazaki,T., Byrne,M.C.,
            Horton,H.F., Fouser,L., Carter,L., Ling,V., Bowman,M.R.,
            Carreno,B.M., Collins,M., Wood,C.R. and Honjo,T.
  TITLE     Engagement of the PD-1 immunoinhibitory receptor by a novel B7
            family member leads to negative regulation of lymphocyte activation
  JOURNAL   J Exp Med 192 (7), 1027-1034 (2000)
   PUBMED   11015443
REFERENCE   10 (bases 1 to 3634)
  AUTHORS   Dong,H., Zhu,G., Tamada,K. and Chen,L.
  TITLE     B7-H1, a third member of the B7 family, co-stimulates T-cell
            proliferation and interleukin-10 secretion
  JOURNAL   Nat Med 5 (12), 1365-1369 (1999)
   PUBMED   10581077
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314567.1, AL162253.17 and
            AI826502.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014143.3.
            
            Summary: This gene encodes an immune inhibitory receptor ligand
            that is expressed by hematopoietic and non-hematopoietic cells,
            such as T cells and B cells and various types of tumor cells. The
            encoded protein is a type I transmembrane protein that has
            immunoglobulin V-like and C-like domains. Interaction of this
            ligand with its receptor inhibits T-cell activation and cytokine
            production. During infection or inflammation of normal tissue, this
            interaction is important for preventing autoimmunity by maintaining
            homeostasis of the immune response. In tumor microenvironments,
            this interaction provides an immune escape for tumor cells through
            cytotoxic T-cell inactivation. Expression of this gene in tumor
            cells is considered to be prognostic in many types of human
            malignancies, including colon cancer and renal cell carcinoma.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK314567.1, SRR1660803.237105.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381577.4/ ENSP00000370989.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-942               AK314567.1         1-942
            943-3399            AL162253.17        13451-15907
            3400-3634           AI826502.1         19-253              c
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p24.1"
     gene            1..3634
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /note="CD274 molecule"
                     /db_xref="GeneID:29126"
                     /db_xref="HGNC:HGNC:17635"
                     /db_xref="MIM:605402"
     exon            1..55
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     exon            56..121
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..942
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; CD274 antigen; programmed cell death 1 ligand
                     1; B7 homolog 1; PDCD1 ligand 1"
                     /codon_start=1
                     /product="programmed cell death 1 ligand 1 isoform a
                     precursor"
                     /protein_id="NP_054862.1"
                     /db_xref="CCDS:CCDS6464.1"
                     /db_xref="GeneID:29126"
                     /db_xref="HGNC:HGNC:17635"
                     /db_xref="MIM:605402"
                     /translation="MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPV
                     EKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQIT
                     DVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGY
                     PKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEE
                     NHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQD
                     TNSKKQSDTHLEET"
     exon            122..463
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     exon            464..751
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     exon            752..859
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..919
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     exon            920..3634
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3539..3544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /note="hexamer: AATAAA"
     polyA_site      3572
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /note="major polyA site"
     regulatory      3609..3614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
                     /note="hexamer: AATAAA"
     polyA_site      3634
                     /gene="CD274"
                     /gene_synonym="B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;
                     PDCD1LG1; PDL1"
ORIGIN      
        1 agttctgcgc agcttcccga ggctccgcac cagccgcgct tctgtccgcc tgcagggcat
       61 tccagaaaga tgaggatatt tgctgtcttt atattcatga cctactggca tttgctgaac
      121 gcatttactg tcacggttcc caaggaccta tatgtggtag agtatggtag caatatgaca
      181 attgaatgca aattcccagt agaaaaacaa ttagacctgg ctgcactaat tgtctattgg
      241 gaaatggagg ataagaacat tattcaattt gtgcatggag aggaagacct gaaggttcag
      301 catagtagct acagacagag ggcccggctg ttgaaggacc agctctccct gggaaatgct
      361 gcacttcaga tcacagatgt gaaattgcag gatgcagggg tgtaccgctg catgatcagc
      421 tatggtggtg ccgactacaa gcgaattact gtgaaagtca atgccccata caacaaaatc
      481 aaccaaagaa ttttggttgt ggatccagtc acctctgaac atgaactgac atgtcaggct
      541 gagggctacc ccaaggccga agtcatctgg acaagcagtg accatcaagt cctgagtggt
      601 aagaccacca ccaccaattc caagagagag gagaagcttt tcaatgtgac cagcacactg
      661 agaatcaaca caacaactaa tgagattttc tactgcactt ttaggagatt agatcctgag
      721 gaaaaccata cagctgaatt ggtcatccca gaactacctc tggcacatcc tccaaatgaa
      781 aggactcact tggtaattct gggagccatc ttattatgcc ttggtgtagc actgacattc
      841 atcttccgtt taagaaaagg gagaatgatg gatgtgaaaa aatgtggcat ccaagataca
      901 aactcaaaga agcaaagtga tacacatttg gaggagacgt aatccagcat tggaacttct
      961 gatcttcaag cagggattct caacctgtgg tttaggggtt catcggggct gagcgtgaca
     1021 agaggaagga atgggcccgt gggatgcagg caatgtggga cttaaaaggc ccaagcactg
     1081 aaaatggaac ctggcgaaag cagaggagga gaatgaagaa agatggagtc aaacagggag
     1141 cctggaggga gaccttgata ctttcaaatg cctgaggggc tcatcgacgc ctgtgacagg
     1201 gagaaaggat acttctgaac aaggagcctc caagcaaatc atccattgct catcctagga
     1261 agacgggttg agaatcccta atttgagggt cagttcctgc agaagtgccc tttgcctcca
     1321 ctcaatgcct caatttgttt tctgcatgac tgagagtctc agtgttggaa cgggacagta
     1381 tttatgtatg agtttttcct atttattttg agtctgtgag gtcttcttgt catgtgagtg
     1441 tggttgtgaa tgatttcttt tgaagatata ttgtagtaga tgttacaatt ttgtcgccaa
     1501 actaaacttg ctgcttaatg atttgctcac atctagtaaa acatggagta tttgtaaggt
     1561 gcttggtctc ctctataact acaagtatac attggaagca taaagatcaa accgttggtt
     1621 gcataggatg tcacctttat ttaacccatt aatactctgg ttgacctaat cttattctca
     1681 gacctcaagt gtctgtgcag tatctgttcc atttaaatat cagctttaca attatgtggt
     1741 agcctacaca cataatctca tttcatcgct gtaaccaccc tgttgtgata accactatta
     1801 ttttacccat cgtacagctg aggaagcaaa cagattaagt aacttgccca aaccagtaaa
     1861 tagcagacct cagactgcca cccactgtcc ttttataata caatttacag ctatatttta
     1921 ctttaagcaa ttcttttatt caaaaaccat ttattaagtg cccttgcaat atcaatcgct
     1981 gtgccaggca ttgaatctac agatgtgagc aagacaaagt acctgtcctc aaggagctca
     2041 tagtataatg aggagattaa caagaaaatg tattattaca atttagtcca gtgtcatagc
     2101 ataaggatga tgcgagggga aaacccgagc agtgttgcca agaggaggaa ataggccaat
     2161 gtggtctggg acggttggat atacttaaac atcttaataa tcagagtaat tttcatttac
     2221 aaagagaggt cggtacttaa aataaccctg aaaaataaca ctggaattcc ttttctagca
     2281 ttatatttat tcctgatttg cctttgccat ataatctaat gcttgtttat atagtgtctg
     2341 gtattgttta acagttctgt cttttctatt taaatgccac taaattttaa attcatacct
     2401 ttccatgatt caaaattcaa aagatcccat gggagatggt tggaaaatct ccacttcatc
     2461 ctccaagcca ttcaagtttc ctttccagaa gcaactgcta ctgcctttca ttcatatgtt
     2521 cttctaaaga tagtctacat ttggaaatgt atgttaaaag cacgtatttt taaaattttt
     2581 ttcctaaata gtaacacatt gtatgtctgc tgtgtacttt gctattttta tttattttag
     2641 tgtttcttat atagcagatg gaatgaattt gaagttccca gggctgagga tccatgcctt
     2701 ctttgtttct aagttatctt tcccatagct tttcattatc tttcatatga tccagtatat
     2761 gttaaatatg tcctacatat acatttagac aaccaccatt tgttaagtat ttgctctagg
     2821 acagagtttg gatttgttta tgtttgctca aaaggagacc catgggctct ccagggtgca
     2881 ctgagtcaat ctagtcctaa aaagcaatct tattattaac tctgtatgac agaatcatgt
     2941 ctggaacttt tgttttctgc tttctgtcaa gtataaactt cactttgatg ctgtacttgc
     3001 aaaatcacat tttctttctg gaaattccgg cagtgtacct tgactgctag ctaccctgtg
     3061 ccagaaaagc ctcattcgtt gtgcttgaac ccttgaatgc caccagctgt catcactaca
     3121 cagccctcct aagaggcttc ctggaggttt cgagattcag atgccctggg agatcccaga
     3181 gtttcctttc cctcttggcc atattctggt gtcaatgaca aggagtacct tggctttgcc
     3241 acatgtcaag gctgaagaaa cagtgtctcc aacagagctc cttgtgttat ctgtttgtac
     3301 atgtgcattt gtacagtaat tggtgtgaca gtgttctttg tgtgaattac aggcaagaat
     3361 tgtggctgag caaggcacat agtctactca gtctattcct aagtcctaac tcctccttgt
     3421 ggtgttggat ttgtaaggca ctttatccct tttgtctcat gtttcatcgt aaatggcata
     3481 ggcagagatg atacctaatt ctgcatttga ttgtcacttt ttgtacctgc attaatttaa
     3541 taaaatattc ttatttattt tgttacttgg tacaccagca tgtccatttt cttgtttatt
     3601 ttgtgtttaa taaaatgttc agtttaacat ccca
//
