LOCUS       NM_001303618           12518 bp    mRNA    linear   PRI 06-DEC-2024
DEFINITION  Homo sapiens CD226 molecule (CD226), transcript variant 2, mRNA.
ACCESSION   NM_001303618 XM_006722373
VERSION     NM_001303618.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Morandi,E., Adoue,V., Bernard,I., Friebel,E., Nunez,N., Aubert,Y.,
            Masson,F., Dejean,A.S., Becher,B., Astier,A., Martinet,L. and
            Saoudi,A.
  TITLE     Impact of the Multiple Sclerosis-Associated Genetic Variant CD226
            Gly307Ser on Human CD8 T-Cell Functions
  JOURNAL   Neurol Neuroimmunol Neuroinflamm 11 (6), e200306 (2024)
   PUBMED   39231385
  REMARK    GeneRIF: Impact of the Multiple Sclerosis-Associated Genetic
            Variant CD226 Gly307Ser on Human CD8 T-Cell Functions.
REFERENCE   2  (bases 1 to 12518)
  AUTHORS   Baker,F.L., Smith,K.A., Mylabathula,P.L., Zuniga,T.M., Diak,D.M.,
            Batatinha,H., Niemiro,G.M., Seckeler,M.D., Pedlar,C.R.,
            O'Connor,D.P., Colombo,J., Katsanis,E. and Simpson,R.J.
  TITLE     Exercise-induced beta2-adrenergic Receptor Activation Enhances the
            Antileukemic Activity of Expanded gammadelta T-Cells via DNAM-1
            Upregulation and PVR/Nectin-2 Recognition
  JOURNAL   Cancer Res Commun 4 (5), 1253-1267 (2024)
   PUBMED   38592213
  REMARK    GeneRIF: Exercise-induced beta2-adrenergic Receptor Activation
            Enhances the Antileukemic Activity of Expanded gammadelta T-Cells
            via DNAM-1 Upregulation and PVR/Nectin-2 Recognition.
REFERENCE   3  (bases 1 to 12518)
  AUTHORS   Kari,S., Bucciarelli,F., Angles,T., Oster,A.C., Cauboue,P.,
            Laviolette,K., Mougenot,M., Morandi,E., Bernard,I., Pignolet,B.,
            Bost,C., Thomas,J., Nogueira,L., Saoudi,A., Liblau,R. and
            Astier,A.L.
  TITLE     Increased levels of circulating soluble CD226 in multiple sclerosis
  JOURNAL   Mult Scler 30 (6), 654-663 (2024)
   PUBMED   38424741
  REMARK    GeneRIF: Increased levels of circulating soluble CD226 in multiple
            sclerosis.
REFERENCE   4  (bases 1 to 12518)
  AUTHORS   Tahara,S., Okumura,G., Matsuo,T., Shibuya,A. and Shibuya,K.
  TITLE     Essential role of CD155 glycosylation in functional binding to
            DNAM-1 on natural killer cells
  JOURNAL   Int Immunol 36 (6), 317-325 (2024)
   PUBMED   38289706
  REMARK    GeneRIF: Essential role of CD155 glycosylation in functional
            binding to DNAM-1 on natural killer cells.
REFERENCE   5  (bases 1 to 12518)
  AUTHORS   Jian,J.L., Zhu,C.S., Xu,Z.W., Ouyang,W.M., Ma,D.C., Zhang,Y.,
            Chen,L.J., Yang,A.G. and Jin,B.Q.
  TITLE     Identification and characterization of the CD226 gene promoter
  JOURNAL   J Biol Chem 281 (39), 28731-28736 (2006)
   PUBMED   16887814
  REMARK    GeneRIF: CD226 promoters P1 and P2 are regulated by Ets-1 and AP-1
REFERENCE   6  (bases 1 to 12518)
  AUTHORS   Shibuya,K., Shirakawa,J., Kameyama,T., Honda,S., Tahara-Hanaoka,S.,
            Miyamoto,A., Onodera,M., Sumida,T., Nakauchi,H., Miyoshi,H. and
            Shibuya,A.
  TITLE     CD226 (DNAM-1) is involved in lymphocyte function-associated
            antigen 1 costimulatory signal for naive T cell differentiation and
            proliferation
  JOURNAL   J Exp Med 198 (12), 1829-1839 (2003)
   PUBMED   14676297
  REMARK    GeneRIF: CD226 is involved in LFA-1-mediated costimulatory signals
            for triggering naive T cell differentiation and proliferation.
REFERENCE   7  (bases 1 to 12518)
  AUTHORS   Kojima,H., Kanada,H., Shimizu,S., Kasama,E., Shibuya,K.,
            Nakauchi,H., Nagasawa,T. and Shibuya,A.
  TITLE     CD226 mediates platelet and megakaryocytic cell adhesion to
            vascular endothelial cells
  JOURNAL   J Biol Chem 278 (38), 36748-36753 (2003)
   PUBMED   12847109
  REMARK    GeneRIF: CD226 mediates platelet and megakaryocytic cell adhesion
            to vascular endothelial cells
REFERENCE   8  (bases 1 to 12518)
  AUTHORS   Bottino,C., Castriconi,R., Pende,D., Rivera,P., Nanni,M.,
            Carnemolla,B., Cantoni,C., Grassi,J., Marcenaro,S., Reymond,N.,
            Vitale,M., Moretta,L., Lopez,M. and Moretta,A.
  TITLE     Identification of PVR (CD155) and Nectin-2 (CD112) as cell surface
            ligands for the human DNAM-1 (CD226) activating molecule
  JOURNAL   J Exp Med 198 (4), 557-567 (2003)
   PUBMED   12913096
  REMARK    GeneRIF: Data show that both PVR and Nectin-2 represent specific
            ligands for the DNAM-1 triggering receptor.
REFERENCE   9  (bases 1 to 12518)
  AUTHORS   Shibuya,K., Lanier,L.L., Phillips,J.H., Ochs,H.D., Shimizu,K.,
            Nakayama,E., Nakauchi,H. and Shibuya,A.
  TITLE     Physical and functional association of LFA-1 with DNAM-1 adhesion
            molecule
  JOURNAL   Immunity 11 (5), 615-623 (1999)
   PUBMED   10591186
REFERENCE   10 (bases 1 to 12518)
  AUTHORS   Shibuya,A., Campbell,D., Hannum,C., Yssel,H., Franz-Bacon,K.,
            McClanahan,T., Kitamura,T., Nicholl,J., Sutherland,G.R.,
            Lanier,L.L. and Phillips,J.H.
  TITLE     DNAM-1, a novel adhesion molecule involved in the cytolytic
            function of T lymphocytes
  JOURNAL   Immunity 4 (6), 573-581 (1996)
   PUBMED   8673704
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091137.23, AK313199.1,
            U56102.1 and AC090231.7.
            
            On Nov 22, 2018 this sequence version replaced NM_001303618.1.
            
            Summary: This gene encodes a glycoprotein expressed on the surface
            of NK cells, platelets, monocytes and a subset of T cells. It is a
            member of the Ig-superfamily containing 2 Ig-like domains of the
            V-set. The protein mediates cellular adhesion of platelets and
            megakaryocytic cells to vascular endothelial cells. The protein
            also plays a role in megakaryocytic cell maturation. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jan 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.200985.1,
                                           SRR1163658.61089.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2155751 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000582621.6/ ENSP00000461947.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AC091137.23        98189-98208         c
            21-129              AK313199.1         1-109
            130-1478            AK313199.1         111-1459
            1479-2588           U56102.1           1220-2329
            2589-2589           AC090231.7         28429-28429
            2590-2836           U56102.1           2331-2577
            2837-12518          AC090231.7         28677-38358
FEATURES             Location/Qualifiers
     source          1..12518
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q22.2"
     gene            1..12518
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /note="CD226 molecule"
                     /db_xref="GeneID:10666"
                     /db_xref="HGNC:HGNC:16961"
                     /db_xref="MIM:605397"
     exon            1..513
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             468..1478
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /note="isoform a precursor is encoded by transcript
                     variant 2; platelet and T cell activation antigen 1; DNAX
                     accessory molecule-1; T lineage-specific activation
                     antigen 1 antigen; CD226 antigen; adhesion glycoprotein"
                     /codon_start=1
                     /product="CD226 antigen isoform a precursor"
                     /protein_id="NP_001290547.1"
                     /db_xref="CCDS:CCDS11997.1"
                     /db_xref="GeneID:10666"
                     /db_xref="HGNC:HGNC:16961"
                     /db_xref="MIM:605397"
                     /translation="MDYPTLLLALLHVYRALCEEVLWHTSVPFAENMSLECVYPSMGI
                     LTQVEWFKIGTQQDSIAIFSPTHGMVIRKPYAERVYFLNSTMASNNMTLFFRNASEDD
                     VGYYSCSLYTYPQGTWQKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPV
                     QAVRWEKIQPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSG
                     LYRCYLQASAGENETFVMRLTVAEGKTDNQYTLFVAGGTVLLLLFVISITTIIVIFLN
                     RRRRRERRDLFTESWDTQKAPNNYRSPISTSQPTNQSMDDTREDIYVNYPTFSRRPKT
                     RV"
     exon            514..849
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            850..1194
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1297
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1352
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..12518
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5540..5545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /note="hexamer: AATAAA"
     polyA_site      5564
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /note="major polyA site"
     regulatory      12499..12504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /note="hexamer: AATAAA"
     polyA_site      12518
                     /gene="CD226"
                     /gene_synonym="DNAM-1; DNAM1; PTA1; TLiSA1"
                     /note="major polyA site"
ORIGIN      
        1 agtgaggact tctctgctcc atgcatgcac cacctttcac ttattattta tatttttaca
       61 tctttatttt agtttctgcc tcctccacat agaatgtaag ttctatcatg gcaatatttt
      121 tttttttttg tattattgta tcctcattgc ctacagctga gttaggcaca gagaatgcat
      181 gcagcacatg tttgttgaat gaatgactcc ttgtagaaaa tgaaagcccg aaaataagcc
      241 gactggttcc ccgagagaga agaaactcaa atctgcccat cctcataaag gagcaaacct
      301 gtcttttaag ctaaggcctt tttcctgtgt tcatacttca gaaaaagaag acacgactga
      361 taacatctga gaggaaggaa actgcattgt gctggggatc tgcatgtcac attataaaaa
      421 gcaatttttt tttttaatag aaccagcctt tcaaacagtt tccagagatg gattatccta
      481 ctttactttt ggctcttctt catgtataca gagctctatg tgaagaggtg ctttggcata
      541 catcagttcc ctttgccgag aacatgtctc tagaatgtgt gtatccatca atgggcatct
      601 taacacaggt ggagtggttc aagatcggga cccagcagga ttccatagcc attttcagcc
      661 ctactcatgg catggtcata aggaagccct atgctgagag ggtttacttt ttgaattcaa
      721 cgatggcttc caataacatg actcttttct ttcggaatgc ctctgaagat gatgttggct
      781 actattcctg ctctctttac acttacccac agggaacttg gcagaaggtg atacaggtgg
      841 ttcagtcaga tagttttgag gcagctgtgc catcaaatag ccacattgtt tcggaacctg
      901 gaaagaatgt cacactcact tgtcagcctc agatgacgtg gcctgtgcag gcagtgaggt
      961 gggaaaagat ccagccccgt cagatcgacc tcttaactta ctgcaacttg gtccatggca
     1021 gaaatttcac ctccaagttc ccaagacaaa tagtgagcaa ctgcagccac ggaaggtgga
     1081 gcgtcatcgt catccccgat gtcacagtct cagactcggg gctttaccgc tgctacttgc
     1141 aggccagcgc aggagaaaac gaaaccttcg tgatgagatt gactgtagcc gagggtaaaa
     1201 ccgataacca atataccctc tttgtggctg gagggacagt tttattgttg ttgtttgtta
     1261 tctcaattac caccatcatt gtcattttcc ttaacagaag gagaaggaga gagagaagag
     1321 atctatttac agagtcctgg gatacacaga aggcacccaa taactataga agtcccatct
     1381 ctaccagtca acctaccaat caatccatgg atgatacaag agaggatatt tatgtcaact
     1441 atccaacctt ctctcgcaga ccaaagacta gagtttaagc ttattcttga catgagtgca
     1501 ttagtaatga ctcttatgta ctcatgcatg gatctttatg caattttttt ccactaccca
     1561 aggtctacct tagatactag ttgtctgaat tgagttactt tgataggaaa aatacttcat
     1621 tacctaaaat catttttcat agaactgttt cagaaaacct gactctaact ggtttatata
     1681 caaaagaaaa cttactgtat catataacag aatgatccag gggagattaa gctttgggca
     1741 agggctattt accagggctt aaatgttgtg tctagaatta agtatgggca taaactggct
     1801 tctgaatccc tttccagagt gttggatcca tttccctggt cttggcctca ctctcatgca
     1861 ggctttcctc ttgtgttggc aagatggctg ccaactcttg gcaattcata catccttgtt
     1921 tctgtctggt agagagtttg cttctcaaat ggagcaaaca aatttgatta ttttttcatt
     1981 gttaaatagg caacatgacc agaaaggatg gaatggctta agtaaactaa gggttcactt
     2041 ctagagctga gaagcagggt caaagcacaa tactgggcaa ttcagagcat ggttagaaga
     2101 ggaaagggga gtctcaaagc tggagagttt accaacaaat attgactgca gtgattaacc
     2161 aagacatttt tgttaactaa aaagtgaaat atgggatgga ttctagaaat ggggtatctc
     2221 tgtccatact tctagaatcc actctatcag catagtccag aagaatacct ggcagtagaa
     2281 gaaatgaata ttcaagagga agataaatgc gagagggcaa tcctttacta ttctcatatt
     2341 tatttatctc tcattctgta tagaattctt gccgccatcc caggtctagc cttaggagca
     2401 aatgtagtag atagtcgaat aataaataac ttaatgtttt ggacatattt tgtctacttt
     2461 tgagaattat ttttaatatg taaattctct caaaagggtc aggcacctag ttattatttt
     2521 ttaatgatta tgtgaaagtt gaatataata taccactaaa agtgacagtt gaaagtggtg
     2581 gcataggacg gtagggtaga aatttgggag ggaaaaaaga aattgggagg gtacaggcaa
     2641 caggagaaag gaatcaaacc acagaaaaat acaaagggaa acttctgctt cactattcag
     2701 acaaagacag ccctaatgac atcaccaaca gtcaaagcaa ttagagacca tacctaatat
     2761 tgtttaaatt ctagatgtag gctaacaatg aaaagtattt gccaaactga ataaaactgt
     2821 catggttacc ttgaaaggac aatggttatt gttaaatata gtgatcattc atgtctaaaa
     2881 gattcattat ttatctctaa agatttctaa agaccaccat ctagaaaaga ttcattatga
     2941 aggctgtatt taaatatcaa agttgtggac ttcatgataa tcttaaataa agcaaatcca
     3001 aattctcctg ttgcctagac agattctaag atgtaattta cacttttaag ctaattagtg
     3061 agtattttat gattttagcc ttaaacacca tgtatgccaa ataatgcact tgttttgtga
     3121 attacagaaa tggtaagtgc ccacatttct gtgaattata aaatttgtga gtttctttta
     3181 acccttttca ggagtgaaaa aataaaaacg accatttcct ggttgtgctt aagtatatgc
     3241 aagaagggta aactctcatt tttattatgt ttgcttaaag atctttttat acctggattc
     3301 atgaaatgtt tccacaaata tattagtgta acaaacttga aaggcagttt acaagaaagc
     3361 actctactat cagatcaatc aaagattctg tgagtgaatt tattggtttg catggtgaag
     3421 caagcttagc atcaattaaa aggtaaataa tttcttttct gaatggtaaa gacaatcaaa
     3481 atattacttt ctggaaaact ccaataacca aattctcaat gattagtgta tgtgagcagg
     3541 aaaacatttt tacagttgta gtatggggaa atataaatcc aattttaaga gagaaaatta
     3601 tgactgggtg tggaagggac agtatagtca gataccattg tcatggtggt ttttactggg
     3661 aacttcatga aagacttttg tagcaaacca ctgcagtatt gcaaagcctc cagaacattt
     3721 ggaacttgtc tctttttcct tgtgtgtgtt tgtgtttttg gtctctcatt caaaatattg
     3781 atgagaacta tttactctgt cctttcttct ctatatattc ttcctctaca gagtgtaggg
     3841 ttttttcagg aatttggagc catctgaagt cctcccaaaa attctctgac gtcttctgat
     3901 gctcctgtta taccctcagg ggtaatgctt gtgaaattcc attcattcat tttctttctc
     3961 tggacatctt tacttaccaa agcactttca ttgtcatctt tttaacatca ttcttaattc
     4021 gtgatagttt tgggactctc cctagtgtat gtttctcccc ctctactctt ttgcacctat
     4081 gattctgatt gttactaaga aagcagatga aaaacagatc cacagaataa acgatcagaa
     4141 ttccagtaaa ttctatttta aatacagata ctttttacaa gttgctgctt tggaagcaaa
     4201 atgcttctta agttttacat atatatatat atatatacat atatatatac acatataatt
     4261 tatatcgatg gataatacat taagaatcta tgcttccttt gaatgccatt aatatttatg
     4321 ttaaagtaac caatgaaagg aaattacttt gttataataa gataggaaga cttgttaatg
     4381 gagtacacag ttttgtcagg gaaagaacac atcttattga actatgatga ctatgcattg
     4441 actatattat tataagagat accttcaaac tttatttaaa gaactttagg tataatatgt
     4501 tgagaaaata aaatagaaat ttcatttact tgtaatcatg cttaaaatgg gaggcaggta
     4561 ggtgaagata taatttttag taaaaactcc aatttatgtt ttaagtaatt cagtgtatta
     4621 ctaaaatact atatatataa acttaaaata catgggttat caatttaaaa gacaaagtaa
     4681 gtaaaaatac ttttagtagg cattcgtgga ttgtgaacat ccaagttata ttggtttgta
     4741 tagaatggca ttaagtaaaa attacagctg tataacagta gttttctaaa ttgagagagt
     4801 ccacattgta attagagatc actgtgacca aaatgcttct ccttgattta taatgatgta
     4861 ctgtattttg tactgcttat atgaaatttc agcaagattg acgatattat aaagatgctt
     4921 ataaagtgta agtggagacg ctaaattgtg agtacaaagt ttctttttca caacagtgat
     4981 aagaaaatat ctttaaaaaa tataagacaa tataaacatg tcatcattag tttagctact
     5041 attaaaatgt aacatctaga aagtactgat ctccaccttc agacttctgt ataagtatat
     5101 tttttcactg atctgttcat tagagttctt ccagccaaga ctctgggctc ttaaaacatg
     5161 tatctgaaaa ctaaaaacaa gttaattttt ttaaaagctt ctctatttct agtgattcaa
     5221 taggtagaaa aatagcttct agaattaact gcaatgcttt ctaaggaaat tttataaatc
     5281 ctcaaggtcg gtttacacat atttttccag attcagagca ctaactatct tgtaagatgt
     5341 aagaaaaggt ccatttggaa gtatgagtaa taaatgtctg ggataattct ggtttatttc
     5401 gtattatcct tgttagaata agttatatgg tcaacctgtt cagaacactt tttctagtgt
     5461 tagtgtgtac ttttggattt ttggttcttg tagggtatag aaatattttc ctttgtcttg
     5521 tattctgttg ttttgaatga ataaaacaca atgtttcacg atcactactt tcatttgcca
     5581 tggagaaata gcagggaaaa atttctacag aataaaatta actgatgaat tacatgcaga
     5641 aaaaattcaa atcaatgata cattgtaatt tttatctcaa tgcaatgttc tttgtatttt
     5701 attttattat tatttttttg agacggagtt tcacttttgt tgcccgggct ggagtgcaat
     5761 ggcacaatct cggctcacca caacctctgc ctcccggatt caagtgattc tcctgcctca
     5821 gcctcctgaa tagctgggat tacaggcata tgccaacatg cctggctaat tttgtatttt
     5881 tagtggagac ggggtttctc cacgttggtc agacttgtct tgaactctgg acctcaggtg
     5941 atccacctgc ctcagcctcc taaattgctg ggattacagg catgagcgac cactcctggc
     6001 cttgttcttt gtattttata agtgcatgta gtgcaaaggg tcaaagggct ttacaggttt
     6061 tttgtttgtt tgtttttgtt tttcccgaaa catagtagtc ccttgccctt cctcattttt
     6121 gttaccttga gacaacaaat tttactactt ctaactcatt attttattta tgttcacttt
     6181 tctgaatagc atgcttatga cactaatact ttttttttca attttagaca ttcattattc
     6241 atttagatgt ctttctctcc ccaaactcac cacataaaat actcttctca tgtctctttc
     6301 agaaatattt gtattaaaat atgattatat caatatttgg catttatttc ttatgacctt
     6361 gccagtactc ttagttaaac tacatggtaa aaatgatttt gctttccctc ctacataact
     6421 ttttttccac ctagagctaa taattgtcat tctggggact gactttttct gtatttacca
     6481 taaattgacc tgaaactccc ctgtgatgca gcaggaattc taccaacgtc aacttcctta
     6541 gaaagactcc attagaagct tgacttgggg ctagaaggag aggcacacaa ctgccatcct
     6601 ggtgtctccc ttcatccaga aaaaggggga ggaatacatg aaacctagaa tccactctaa
     6661 aacattttcc agaacaaaag gacatgtgtt tccgtgttgt aaatgtttaa cgagtgccca
     6721 taacaaggaa taataagtct attatgtttg cttttgtgtc tgtaaaagtt gggggtattg
     6781 gttgtaagca cgaaaacaga tactgactgt tgaagaaaaa aaaaaatacg aggtcaggag
     6841 tttgagacca acttggccaa tatggtgaaa ccctgtctta gtaaaaatag aaaaattagc
     6901 caggcctggt ggcacgcacc tgtagtccca gctacttggg aggctgaggc agaagaatcg
     6961 cttgaacccg ggaggcagag gttgcagtga gccaagatcg caccactgca ctccaccctg
     7021 ggcaacagag cgagactccg tctcaaaaaa aaaaaaaaaa aaaaaaaaaa aaagttaagt
     7081 atttgaacat aggggtggct catagaattc ccaggacacc cgatggagta ggcttgcaaa
     7141 acacaacatg tggcaactcc agtgggaaac gaggcaggaa acactcgttt cctgcagaaa
     7201 gcaacaattt gggcttcgat accctcccta gaacacaggg cagtgaatct gagcagcatc
     7261 agtaccccac gttcggatga gtcctgagcc cctattttta ttcactgact tattccaaaa
     7321 tcagtgtctc ttaaatatat ctggaaggca gcagcttgta tctccccctt cagcttccat
     7381 agtggcagtc agggtacaac ttactttcca aacagaacac actgcgacat tccctccagg
     7441 ctcgttgaag aacttcaact gacaaatgtc cctcctcgac cagatgatag ttttcttaaa
     7501 ggcagggttt aatataccct tttataaatg tttcaaggcc ctgtgtaata cctgagttta
     7561 ttccagatgt aactaaatat atccaagatt gttttaaaat aaattgctga aaaaacaaat
     7621 aaatacagtt agtatctata tcaatattct cagttggcag ttttgcaata atggccgata
     7681 gttcattttt agtaacacta ttgacattgc atttggatat tagggtttac taatcatccg
     7741 catgtataca ttgcatattt ttctagactt taactttatt caaatctatt gatttttaaa
     7801 cctgcaactt atgtctagac acaggtatac ctttacaaga actaccattt tttttggtaa
     7861 catactacct ccaaaatttc aagtaagaag ttgatttttg tccattttta aatggaaaac
     7921 ttgtaatcaa aatgccacaa aattatactg tgtatcattt gacctataga aaccaatatt
     7981 attacaggaa gaaagcagag ccaatcttct acctgtggtc aaataagtgg aggccctttc
     8041 tagactaagt tctcatgagt ttaaaatacc aagcataagt tctccaaatt cctgaaaagg
     8101 aagccttgtg ttgtattgcc cagccatatt tgtaagacat aaaaataaaa cttgagaaga
     8161 agctatgata acttactttc ttcattcttc aaaatttaca taatctcaac tgattttatg
     8221 tttttatgaa aatgcattct taagatatat ccttattcaa tcatgtattc attacatcct
     8281 ttatgccagg tatccaaaag tacttacagt gactaagacc attattcttt gatcagctgc
     8341 ctgagtaaga ctttgagctc tccaatatac tctcagtgat actaagtttt ctgagtaaca
     8401 gctttggatg tggcttcagt tgagctgatt tatcccacac tttattttta tcgtataatg
     8461 gtcctcagaa gcaaattttg attttagctc acataaaaaa tgtacaaaga aatgtaatgg
     8521 ctcagtagct tctagagata gagattactc ttctaacctt tctgtaattt tgtatgtcta
     8581 ttttataatt ctttcaatgt ctaatgaata gctatctttt tttgagacgg agtctcgctc
     8641 tgtcgcccag gctggagtgc agtggtgcga cctcggctca ccgcaagctg cgtcttccag
     8701 gttcacgcca ttctcctgcc tcagcctccc gagtagctgg gacttcaggc gcccaccacc
     8761 atgcccagct aatttttttg tatttttagt agagacgggg tttcaccgtg ttagccaggg
     8821 tggtctcgat ctcctgacct cgtgatccgc ccgcctcggc ctcccaacgt gctgggatta
     8881 caggagtgag ccaccgcgcc cggcctcctt agtttcttaa ggtggaagcc tagattattg
     8941 attttatatg ttgttttctt ttccaatagt ggcacttaat gctataaatt tcactttgtt
     9001 ccacaagttt tggtaagctc tatttttatt ttcatttagt ccaaaatatt ttaaaatttc
     9061 ttttgatatt tcttctttga gccatgaatt atttacaatg tgttgtttaa tctctatata
     9121 ttttgggatt tttctacttt atatctctta cagatttcta acttaatttc atcatgtttt
     9181 aaaaacattc tttgtataat ttctattctt ttaaattttt caggtgtatt ttatggccca
     9241 gaatatggtc tatcttgtag aatgtttcat gtgatcttaa gaagaatgtt cattctgctg
     9301 ttgagtgtaa tattctacaa atgtccatta gattaaactg attgatacca ccgttcagat
     9361 tatctatatc ctttctgatt ttccctcttc ttgatctatc acatactgac agatcaagtg
     9421 atcaagtctc gttaaagact gcaagtaaaa tagtggattt ttctatttct ccttgcagtt
     9481 ttgttagttt ttgtctcatg tatcttgata ctcttgttag tacatatact ttcagaatcg
     9541 ttaggttttc ttggagaatt gaccccttta ccacatgtaa tgtccctttt attcttgata
     9601 atctttcttg ttctgtctgc tttttctgat attaacataa ctttcagttt tttaaaaaat
     9661 taacattagc atctcacatc tttatccttt taattttaaa ttatctaaat atttatattt
     9721 aatgtgcctt tcttatagac aatgtatagt tgcgtctatt tgtaatttcc ccacttttct
     9781 tacttaaaaa tgttgtagat atataggagt tgtatatatt tggggggtac atgtgatgtt
     9841 ttgatacctg tatacaatat gtaatgatca tattgggtaa tcgtgatatc tgtcacctct
     9901 aacattcatc ttttttgtgt gtttaaaccc accacttcta attggtacat ttagattatt
     9961 caaatttaag tgattattga tatagttgga ttaatatcta ctatgtttgt aacttttcta
    10021 tccttgcact cgttctttct tttttatcct cctttttctg tgttctctga ttttaactgg
    10081 ggtttttaca tgatttaatt ttctctcgtg gcatatcttt cattgatcaa cctaggtttt
    10141 tctccttttc ccctcttttt tttggtattt attctattta gtgttatctg agctacctga
    10201 gttggtgtct atcactaatt ttggcaagtt cccagacgtt attacttcta acattctttt
    10261 gctccattct ttcttcttct tcaattattc catagtcttg aatattctgg gtttttccca
    10321 ctctttgaat tttagtttga aaagtttcta ttggcctagc ttcaaagtca ttcattcttc
    10381 cttcggggtt ccaagtcaac tgataattgc atcaaagata tccttccttt ctattactat
    10441 gttttttatt gctaccattt cttttttatt ccttcttagt gtttccatct ttcttcttac
    10501 attatccatc tgttgtctat ttttttcatg agagctctta acatattaat gataagttcc
    10561 atgtctgata attctgacac gtgtcatgtc tctatctggt tccaatgatt gctttatctc
    10621 ttcagaccat gacttttctt gccttttgac gttctttgac attttttttg aattttttgt
    10681 tgcaagccag atctggtgtg ttatgtaata ggaacaggta aataagtctt tagcttgcag
    10741 acttatctta atctgactaa ctattagact gtgtttaaag tctgttataa ccataggtgc
    10801 taaatttctt caaattcctc tagtgtcttt gttttgtttg ttcatgtgtt tttccccttc
    10861 ttgagttcag gcttccctaa gtgctcctct tcagagagac tttctgtctt tcagctcttt
    10921 cctctgcaat tcactgttac tatactggag ccctgttggt gtagtactaa gctgtgggaa
    10981 aggagagtgc tctgtaatct tacagtgaaa tctcagtctt ttagtgggtc tgtgtctggg
    11041 acattcacag agcttctcca gtggtattgc ttcctcatcc tcaactctct ttcctggctg
    11101 cagcattccc aatgtatttc tttgaaggcc tgccccctgt tgactgttat tttccctctt
    11161 tccttaagtg ggacagggag acttcagggg ctgggatgag gtttgggaat tgtgcttggc
    11221 agagtccttt ccatctttgt taccaagaag gttcatggct tatttctcaa tggatgtccc
    11281 tctctatctg ttgccagagc cacgaggaaa tttttcttgg atcctcataa tgagaacctt
    11341 ggagtttcct actggaaaag cccttgaatg tgtggagtgc ctcaagagca cagcccccat
    11401 gggtttcttg ctcacaccag tccacaaaca gatgccagca attcacccaa cttaccatat
    11461 aaaggctcat actagtttat ggctccagtg ctttgactcc agataaatgg ctattggttg
    11521 cgtatctctc tggatgtatc tgtatctcca gattttgggg tggcagtttg ctcaggacct
    11581 tggttctcta ataggtctaa taagaaaagt cattgatttt cagctttcca actttccagc
    11641 tttgtcttgt tataagcatg gcagcaacat cttccatgcc ttaacatgat gacactaaag
    11701 gcagaagtcg atctccatgt ataaacattt taacacatat gttttttgtt atcgtggttt
    11761 ctgacctgtc tctttgccct gactttctga tactgcacta gggttcctgt tgctggactc
    11821 cattccatat gacttgctct cgtctaggct gctctttggc tcatctttat aaatcatgat
    11881 ccaaaatgaa gcacatattt attttttaaa taaatatgaa atgaagtata gacatcaaac
    11941 tgaagatgag tagatcatac tgagtttcac tgtctgtgct tggatcaaca tcaggcctta
    12001 tacaaatatt caagtccaga ggcaaaaggt aataaggaaa atttgtagca caagccacaa
    12061 ggagataaca tgtcaagtct atgcgattgg aaataaacta aagatgaact gctggggatg
    12121 ctcactcatc acagagctca gtctaaagca ccagatttca caagcatttt ttgggggaaa
    12181 ttctgttaaa atgaaatatg agtcacatgg tggtgtttca ctcatcatat gtgttcaata
    12241 ttaattcatt ttaaggttta gttgcacaaa aggtaaatga gaattagaag actccatggg
    12301 taagaggagc cacagaagta aagcattgtc aagggttcta tgtctatata tttagatatt
    12361 aggcttctga gaaaaaaaca caataggaag gaagatgaac acaacagagg gcagaaggtc
    12421 tatacgtcct gaggcctttt atgcaacgtt tgtttgtgga atgtttttta agaatgtgtg
    12481 agagtcattt taatgtgaaa taaagaccta cgtctaca
//
