LOCUS       NM_133493               9031 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.
ACCESSION   NM_133493
VERSION     NM_133493.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Zhou,F., Wang,L., Ge,H., Zhang,D. and Wang,W.
  TITLE     H3K27 acetylation activated-CD109 evokes 5-fluorouracil resistance
            in gastric cancer via the JNK/MAPK signaling pathway
  JOURNAL   Environ Toxicol 38 (12), 2857-2866 (2023)
   PUBMED   37661780
  REMARK    GeneRIF: H3K27 acetylation activated-CD109 evokes 5-fluorouracil
            resistance in gastric cancer via the JNK/MAPK signaling pathway.
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Kim,J.S., Shin,M.J., Lee,S.Y., Kim,D.K., Choi,K.U., Suh,D.S.,
            Kim,D. and Kim,J.H.
  TITLE     CD109 Promotes Drug Resistance in A2780 Ovarian Cancer Cells by
            Regulating the STAT3-NOTCH1 Signaling Axis
  JOURNAL   Int J Mol Sci 24 (12), 10306 (2023)
   PUBMED   37373457
  REMARK    GeneRIF: CD109 Promotes Drug Resistance in A2780 Ovarian Cancer
            Cells by Regulating the STAT3-NOTCH1 Signaling Axis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9031)
  AUTHORS   Mori,N., Esaki,N., Shimoyama,Y., Shiraki,Y., Asai,N., Sakai,T.,
            Nishida,Y., Takahashi,M., Enomoto,A. and Mii,S.
  TITLE     Significance of expression of CD109 in osteosarcoma and its
            involvement in tumor progression via BMP signaling
  JOURNAL   Pathol Res Pract 245, 154443 (2023)
   PUBMED   37030166
  REMARK    GeneRIF: Significance of expression of CD109 in osteosarcoma and
            its involvement in tumor progression via BMP signaling.
REFERENCE   4  (bases 1 to 9031)
  AUTHORS   Starodubtseva,M.N., Liu,J., Nadyrov,E.A., Shkliarava,N.M.,
            Sadouskaya,A.U., Starodubtsev,I.E., Achinovich,S.L., Meng,X.,
            Zinovkin,D.A. and Pranjol,M.Z.I.
  TITLE     CD109-regulated mechanical properties of endothelial cells
  JOURNAL   Cytoskeleton (Hoboken) 80 (5-6), 123-132 (2023)
   PUBMED   36929132
  REMARK    GeneRIF: CD109-regulated mechanical properties of endothelial
            cells.
REFERENCE   5  (bases 1 to 9031)
  AUTHORS   Solomon,K.R., Sharma,P., Chan,M., Morrison,P.T. and Finberg,R.W.
  TITLE     CD109 represents a novel branch of the
            alpha2-macroglobulin/complement gene family
  JOURNAL   Gene 327 (2), 171-183 (2004)
   PUBMED   14980714
REFERENCE   6  (bases 1 to 9031)
  AUTHORS   Giesert,C., Marxer,A., Sutherland,D.R., Schuh,A.C., Kanz,L. and
            Buhring,H.J.
  TITLE     Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34-
            hematopoietic and mesenchymal stem cell subsets
  JOURNAL   Ann N Y Acad Sci 996, 227-230 (2003)
   PUBMED   12799300
REFERENCE   7  (bases 1 to 9031)
  AUTHORS   Schuh,A.C., Watkins,N.A., Nguyen,Q., Harmer,N.J., Lin,M.,
            Prosper,J.Y., Campbell,K., Sutherland,D.R., Metcalfe,P.,
            Horsfall,W. and Ouwehand,W.H.
  TITLE     A tyrosine703serine polymorphism of CD109 defines the Gov platelet
            alloantigens
  JOURNAL   Blood 99 (5), 1692-1698 (2002)
   PUBMED   11861285
  REMARK    GeneRIF: A tyrosine703serine polymorphism of CD109 defines the Gov
            platelet alloantigens.
REFERENCE   8  (bases 1 to 9031)
  AUTHORS   Lin,M., Sutherland,D.R., Horsfall,W., Totty,N., Yeo,E., Nayar,R.,
            Wu,X.F. and Schuh,A.C.
  TITLE     Cell surface antigen CD109 is a novel member of the alpha(2)
            macroglobulin/C3, C4, C5 family of thioester-containing proteins
  JOURNAL   Blood 99 (5), 1683-1691 (2002)
   PUBMED   11861284
  REMARK    GeneRIF: Cell surface antigen CD109 is a novel member of the
            alpha(2) macroglobulin/C3, C4, C5 family of thioester-containing
            proteins. cDNA sequence determination and protein structure
            analysis given.
REFERENCE   9  (bases 1 to 9031)
  AUTHORS   Lucas,G.F. and Metcalfe,P.
  TITLE     Platelet and granulocyte glycoprotein polymorphisms
  JOURNAL   Transfus Med 10 (3), 157-174 (2000)
   PUBMED   10972910
  REMARK    Review article
REFERENCE   10 (bases 1 to 9031)
  AUTHORS   Kelton,J.G., Smith,J.W., Horsewood,P., Humbert,J.R., Hayward,C.P.
            and Warkentin,T.E.
  TITLE     Gova/b alloantigen system on human platelets
  JOURNAL   Blood 75 (11), 2172-2176 (1990)
   PUBMED   2346781
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF410459.1, AK095888.1,
            AL834478.1, BX646244.1, BX107999.1, CB529476.1, BM722643.1,
            AK130104.1 and CB853216.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_133493.4.
            
            Summary: This gene encodes a glycosyl phosphatidylinositol
            (GPI)-linked glycoprotein that localizes to the surface of
            platelets, activated T-cells, and endothelial cells. The protein
            binds to and negatively regulates signalling by transforming growth
            factor beta (TGF-beta). Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Apr 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF410459.1, EF553520.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000287097.6/ ENSP00000287097.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-657               AF410459.1         100-756
            658-1976            AK095888.1         710-2028
            1977-3734           AF410459.1         2076-3833
            3735-4501           AL834478.1         1949-2715
            4502-5058           BX646244.1         1-557
            5059-5638           BX107999.1         40-619
            5639-5764           CB529476.1         10-135              c
            5765-6251           BM722643.1         123-609
            6252-7586           AK130104.1         429-1763
            7587-9030           AK130104.1         1768-3211
            9031-9031           CB853216.1         22-22               c
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q13"
     gene            1..9031
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /note="CD109 molecule"
                     /db_xref="GeneID:135228"
                     /db_xref="HGNC:HGNC:21685"
                     /db_xref="MIM:608859"
     exon            1..87
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..4351
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; Gov platelet alloantigens; activated T-cell
                     marker CD109; platelet-specific Gov antigen; 150 kDa
                     TGF-beta-1-binding protein; C3 and PZP-like
                     alpha-2-macroglobulin domain-containing protein 7; CD109
                     antigen"
                     /codon_start=1
                     /product="CD109 antigen isoform 1 preproprotein"
                     /protein_id="NP_598000.2"
                     /db_xref="CCDS:CCDS4982.1"
                     /db_xref="GeneID:135228"
                     /db_xref="HGNC:HGNC:21685"
                     /db_xref="MIM:608859"
                     /translation="MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTI
                     GVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIY
                     ELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPY
                     KTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQS
                     FQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGK
                     KKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGI
                     SRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVIT
                     VTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGS
                     KSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVA
                     VGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKV
                     KAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLG
                     MFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGS
                     SPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPV
                     ELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNE
                     INATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGI
                     EKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASL
                     IRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDG
                     SFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDP
                     GRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYT
                     LALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAA
                     YALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTN
                     IQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYN
                     VKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNL
                     LSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDAS
                     VSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFK
                     LLYFMELWL"
     exon            88..260
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            261..289
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            290..520
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            521..646
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            647..686
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            687..771
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            772..868
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1010
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1120
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1345
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1447
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1510
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1687
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1840
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1915
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..1976
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2118
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2236
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2350
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2351..2569
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2714
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2891
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..2973
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            2974..3202
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            3203..3368
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            3369..3559
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3715
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            3716..3781
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            3782..3924
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..4072
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4175
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     exon            4176..9031
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4629..4634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /note="hexamer: AATAAA"
     polyA_site      4649
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
     regulatory      9008..9013
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /note="hexamer: AATAAA"
     polyA_site      9031
                     /gene="CD109"
                     /gene_synonym="CPAMD7; p180; r150"
                     /note="major polyA site"
ORIGIN      
        1 agacgccgtc gagatgcagg gcccaccgct cctgaccgcc gcccacctcc tctgcgtgtg
       61 caccgccgcg ctggccgtgg ctcccgggcc tcggtttctg gtgacagccc cagggatcat
      121 caggcccgga ggaaatgtga ctattggggt ggagcttctg gaacactgcc cttcacaggt
      181 gactgtgaag gcggagctgc tcaagacagc atcaaacctc actgtctctg tcctggaagc
      241 agaaggagtc tttgaaaaag gctcttttaa gacacttact cttccatcac tacctctgaa
      301 cagtgcagat gagatttatg agctacgtgt aaccggacgt acccaggatg agattttatt
      361 ctctaatagt acccgcttat catttgagac caagagaata tctgtcttca ttcaaacaga
      421 caaggcctta tacaagccaa agcaagaagt gaagtttcgc attgttacac tcttctcaga
      481 ttttaagcct tacaaaacct ctttaaacat tctcattaag gaccccaaat caaatttgat
      541 ccaacagtgg ttgtcacaac aaagtgatct tggagtcatt tccaaaactt ttcagctatc
      601 ttcccatcca atacttggtg actggtctat tcaagttcaa gtgaatgacc agacatacta
      661 tcaatcattt caggtttcag aatatgtatt accaaaattt gaagtgactt tgcagacacc
      721 attatattgt tctatgaatt ctaagcattt aaatggtacc atcacggcaa agtatacata
      781 tgggaagcca gtgaaaggag acgtaacgct tacattttta cctttatcct tttggggaaa
      841 gaagaaaaat attacaaaaa catttaagat aaatggatct gcaaacttct cttttaatga
      901 tgaagagatg aaaaatgtaa tggattcttc aaatggactt tctgaatacc tggatctatc
      961 ttcccctgga ccagtagaaa ttttaaccac agtgacagaa tcagttacag gtatttcaag
     1021 aaatgtaagc actaatgtgt tcttcaagca acatgattac atcattgagt tttttgatta
     1081 tactactgtc ttgaagccat ctctcaactt cacagccact gtgaaggtaa ctcgtgctga
     1141 tggcaaccaa ctgactcttg aagaaagaag aaataatgta gtcataacag tgacacagag
     1201 aaactatact gagtactgga gcggatctaa cagtggaaat cagaaaatgg aagctgttca
     1261 gaaaataaat tatactgtcc cccaaagtgg aacttttaag attgaattcc caatcctgga
     1321 ggattccagt gagctacagt tgaaggccta tttccttggt agtaaaagta gcatggcagt
     1381 tcatagtctg tttaagtctc ctagtaagac atacatccaa ctaaaaacaa gagatgaaaa
     1441 tataaaggtg ggatcgcctt ttgagttggt ggttagtggc aacaaacgat tgaaggagtt
     1501 aagctatatg gtagtatcca ggggacagtt ggtggctgta ggaaaacaaa attcaacaat
     1561 gttctcttta acaccagaaa attcttggac tccaaaagcc tgtgtaattg tgtattatat
     1621 tgaagatgat ggggaaatta taagtgatgt tctaaaaatt cctgttcagc ttgtttttaa
     1681 aaataagata aagctatatt ggagtaaagt gaaagctgaa ccatctgaga aagtctctct
     1741 taggatctct gtgacacagc ctgactccat agttgggatt gtagctgttg acaaaagtgt
     1801 gaatctgatg aatgcctcta atgatattac aatggaaaat gtggtccatg agttggaact
     1861 ttataacaca ggatattatt taggcatgtt catgaattct tttgcagtct ttcaggaatg
     1921 tggactctgg gtattgacag atgcaaacct cacgaaggat tatattgatg gtgtttatga
     1981 caatgcagaa tatgctgaga ggtttatgga ggaaaatgaa ggacatattg tagatattca
     2041 tgacttttct ttgggtagca gtccacatgt ccgaaagcat tttccagaga cttggatttg
     2101 gctagacacc aacatgggtt acaggattta ccaagaattt gaagtaactg tacctgattc
     2161 tatcacttct tgggtggcta ctggttttgt gatctctgag gacctgggtc ttggactaac
     2221 aactactcca gtggagctcc aagccttcca accatttttc atttttttga atcttcccta
     2281 ctctgttatc agaggtgaag aatttgcttt ggaaataact atattcaatt atttgaaaga
     2341 tgccactgag gttaaggtaa tcattgagaa aagtgacaaa tttgatattc taatgacttc
     2401 aaatgaaata aatgccacag gccaccagca gacccttctg gttcccagtg aggatggggc
     2461 aactgttctt tttcccatca ggccaacaca tctgggagaa attcctatca cagtcacagc
     2521 tctttcaccc actgcttctg atgctgtcac ccagatgatt ttagtaaagg ctgaaggaat
     2581 agaaaaatca tattcacaat ccatcttatt agacttgact gacaataggc tacagagtac
     2641 cctgaaaact ttgagtttct catttcctcc taatacagtg actggcagtg aaagagttca
     2701 gatcactgca attggagatg ttcttggtcc ttccatcaat ggcttagcct cattgattcg
     2761 gatgccttat ggctgtggtg aacagaacat gataaatttt gctccaaata tttacatttt
     2821 ggattatctg actaaaaaga aacaactgac agataatttg aaagaaaaag ctctttcatt
     2881 tatgaggcaa ggttaccaga gagaacttct ctatcagagg gaagatggct ctttcagtgc
     2941 ttttgggaat tatgaccctt ctgggagcac ttggttgtca gcttttgttt taagatgttt
     3001 ccttgaagcc gatccttaca tagatattga tcagaatgtg ttacacagaa catacacttg
     3061 gcttaaagga catcagaaat ccaacggtga attttgggat ccaggaagag tgattcatag
     3121 tgagcttcaa ggtggcaata aaagtccagt aacacttaca gcctatattg taacttctct
     3181 cctgggatat agaaagtatc agcctaacat tgatgtgcaa gagtctatcc attttttgga
     3241 gtctgaattc agtagaggaa tttcagacaa ttatactcta gcccttataa cttatgcatt
     3301 gtcatcagtg gggagtccta aagcgaagga agctttgaat atgctgactt ggagagcaga
     3361 acaagaaggt ggcatgcaat tctgggtgtc atcagagtcc aaactttctg actcctggca
     3421 gccacgctcc ctggatattg aagttgcagc ctatgcactg ctctcacact tcttacaatt
     3481 tcagacttct gagggaatcc caattatgag gtggctaagc aggcaaagaa atagcttggg
     3541 tggttttgca tctactcagg ataccactgt ggctttaaag gctctgtctg aatttgcagc
     3601 cctaatgaat acagaaagga caaatatcca agtgaccgtg acggggccta gctcaccaag
     3661 tcctgtaaag tttctgattg acacacacaa ccgcttactc cttcagacag cagagcttgc
     3721 tgtggtacag ccaacggcag ttaatatttc cgcaaatggt tttggatttg ctatttgtca
     3781 gctcaatgtt gtatataatg tgaaggcttc tgggtcttct agaagacgaa gatctatcca
     3841 aaatcaagaa gcctttgatt tagatgttgc tgtaaaagaa aataaagatg atctcaatca
     3901 tgtggatttg aatgtgtgta caagcttttc gggcccgggt aggagtggca tggctcttat
     3961 ggaagttaac ctattaagtg gctttatggt gccttcagaa gcaatttctc tgagcgagac
     4021 agtgaagaaa gtggaatatg atcatggaaa actcaacctc tatttagatt ctgtaaatga
     4081 aacccagttt tgtgttaata ttcctgctgt gagaaacttt aaagtttcaa atacccaaga
     4141 tgcttcagtg tccatagtgg attactatga gccaaggaga caggcggtga gaagttacaa
     4201 ctctgaagtg aagctgtcct cctgtgacct ttgcagtgat gtccagggct gccgtccttg
     4261 tgaggatgga gcttcaggct cccatcatca ctcttcagtc atttttattt tctgtttcaa
     4321 gcttctgtac tttatggaac tttggctgtg atttattttt aaaggactct gtgtaacact
     4381 aacatttcca gtagtcacat gtgattgttt tgttttcgta gaagaatact gcttctattt
     4441 tgaaaaaaga gttttttttc tttctatggg gttgcaggga tggtgtacaa caggtcctag
     4501 catgtatagc tgcatagatt tcttcacctg atctttgtgt ggaagatcag aatgaatgca
     4561 gttgtgtgtc tatattttcc cctctcaaaa tcttttagaa tttttttgga ggtgtttgtt
     4621 ttctccagaa taaaggtatt actttagaat aggtattctc ctcattttgt gaaagaaatg
     4681 aacctagatt cttaagcatt attacacatc catgtttgct taaagatgga tttccctggg
     4741 aatgggagaa aacagccagc aggaggagct tcatctgttc ccttcccacc tccaacctag
     4801 ccctactgcc caccccaccc caacccaccc catgcccagt ggtctcagta gatacttctt
     4861 aactggaaat tctttctttt cagaatctag gtggtgaatt ttttttaagt ggcacggtct
     4921 ttttctgctt gaaatctgat cacacccccc agccattgcc ctccctctct ttttcctctg
     4981 tagagaaatg tgaggggcag tacatttact gtgcttttca caccatctca gaggttgagg
     5041 agcatactga aaattgccct ggggggtgct gggtgtgctg tctccttccc acatcctcag
     5101 ccccacacca gctctatttc aggggtgaga gtcagagagc actgcaatat gtgcttcatg
     5161 ggatttcgat tcgaagatcc tagaccaggg agacactgtg agccagggat acaacaaaat
     5221 actaggtaag tcactgcaga ccgacctccc tgcagtttgg gaaagaagct gggtttgtgg
     5281 agaatcagag catcttgaca tgactgctga cctaaagatc cctggcattg gccagggatc
     5341 ctgtggaacc tcttctagtt caggggtgtg agcattagac tgccagttgt ctagtgacat
     5401 ctgatgcttg ctgtgaactt ttaagatccc cgaatcctga gcacctcaat ctttaattgc
     5461 cctgtattcc gaagggtaat ataatttatc tggatggaaa ttttaaagat gaatccccct
     5521 tttttctttt cttctctctt ttctttcctt ctccctttct tctttgcctt ctaaatatac
     5581 tgaaatgatt tagatatgtg tcaacaatta atgatctttt attcaatcta agaaatggtt
     5641 tagtttttct ctttagctct atggcatttc actcaagtgg acaggggaaa aagtaattgc
     5701 catgggctcc aaagaatttg ctttatgttt ttagctattt aaaaataaat ccatcaaaaa
     5761 taaagtatgc aaatgtatct tttaaagtta atttttaaaa atgctcttat tttagtgaat
     5821 tttcagaaat tatagtggaa tggatgctca tatattgctt atggatattt tggataccaa
     5881 agtaggaata actgacattc agtattttaa agctggcaaa cctgtacata gaaaatagat
     5941 ccccagacag tggtctatga agagggcagt taagtatcaa atacttaatt ttcttgcctt
     6001 tttttcttaa gtggggaaaa gtttctagat ctcttacacc tctgacacaa tctgttctaa
     6061 aacaggcact tgtaatgttg gggcctcctt gtaaacgtgt ttttgccctt tactctctgg
     6121 gagttcttta aaggtgaaat catcttacaa agaaattggg ggagggtctt ggcaaaggac
     6181 tttcccctcc tctttcctgg cctgggaacc ttatactgac aatcaatact ttatatttta
     6241 aagtatataa tttatagtta acttctagtg taatatatta ggaaacacta gaatggaaag
     6301 gccattggaa gacaggttgt atctttttta gaccatattt ccttgtttaa aaactatcat
     6361 ttgaatactt ttttggtgaa gaactccatg ttttcaagtt aaaggtcacc tcgtaggcca
     6421 ggcgcagtgg ctcatgcctg taatcccagc actctgggag gctgaggcgg gtgaatcaca
     6481 aggttaggag tttgagacca gcctggccaa tatggtgaaa ccccgtccct actaaaaata
     6541 caaaatttag ccaggcgtgg tggcatgcac ctgtagtccc acctactcgg gaggctgagg
     6601 caggagaatc acttgaacct gagagacaga ggttgcagtg agccgagatc acgccactgc
     6661 actccagcct gggggacaga gtgagattct gtctcaaaaa acaaaaaaca aaaaagtcac
     6721 cttgtaactc atctcttttt attgtaagtt tattaaaaat gaagaggaca acaatgagaa
     6781 ggaacataaa gggttagcta gcactgtctc ctggtgcatg gggctgtgca gatgtcccgg
     6841 ccacttcttc cttcatactt cccttagaga acttgctctg ctacaagcag tgggcttgga
     6901 ctaaaagtga ttaaaatacc acaggcataa ggagaaaagg agtatatgta gtagtaataa
     6961 ttactagtat aaattatttt cttcacatgc tatgagtaat aatattaaaa aactcatttt
     7021 accattaaga ttccttatgc tgaagctctt ccatttagaa tactgtcaat gtcatttact
     7081 ggtatgaact aaagtccccc ttcttttcca ctcactggga accttagtaa aacaccagca
     7141 tatcttacct ctctttctga ctggccgatg cttccagaga ctgaatgttg ggaaaaccta
     7201 gtagccaaac aattctagga cagaataaca tttttatatt tggttccacc atcttattac
     7261 atttagttat agttttaaaa aagaaattca agcccattaa aatatgtctg gtcaatgaaa
     7321 tgcttccttt tattgtgttg tgctattgta ctttgttttt caaaacattg taaaaatagt
     7381 atctttggtt tagtattttg gattatatat tataatctga ggagtgtttt gcttatgtag
     7441 aatccagata tatttctgtt acctaggaga tgttacttac atatgtaata ctgtatcctg
     7501 cacgtggaaa tattcagaat tgtagatagc ataactctcc ctgctcctat tcttttgagc
     7561 ctaggtataa tttttttttt ttttttagaa aaagacatat ttagctttaa tttctattta
     7621 tgctaaacat atttataagt agtctgtcaa tataatacca actattttta tttttacata
     7681 attcaattat ttcatttgac atgtctggca gactcaagac attaagtaaa aaattggaac
     7741 tatgattttt ctttgtcatt ttttaaaaaa gaattatttt attaacctgc tggcatataa
     7801 tctggagttc ttttcacaac cttacttttt ctgatttgct ttattgaatg attgaatact
     7861 catttctttc taaaaatatg ttgtaaattc tcccttggca agatttctcc ctatgagggt
     7921 agttattatt tgagtctgcc aagtggttac catggggcaa ggtgccatga tgtattcttg
     7981 ggtgcattgg ttttttgcgc attgtaaatt taagacactt atagtaagtg gactcattca
     8041 tagatgagtt tcagaacctt ttacgttctc ggtagaggct tctgtcggac aggcagaaga
     8101 gtgtattcct cacttttttt tttgtcttca aattccagta aggcatagca cttttaagaa
     8161 attagaattt ttctatcatc tatgcaaatg atatttatgt taatattaaa tatcttatgt
     8221 tacactggga gtaatttgag gtgcaattat ttttattact actttgaata gaggaccatt
     8281 atccttcttt cttcagaaaa ctaagaagta agtgtaactt ttaaagtaag tatatatcag
     8341 tgagagtagg cttgttttac aactatttct agccagtgag ttgtgttttc atgtctcatc
     8401 aaaagacaat accacattgc atcattttac aaaatatgtt gtcattttca tttcagttgt
     8461 aacataggaa aatagatatt tcctagatga tttctgagtt tcttactgca aagaacagtt
     8521 ataaattggt atacatgtgt ctctgtaata gggataatat tgatatatct gttgctacat
     8581 atttaagaat cattctatct tatgttgtct tgaggccaag atttaccacg tttgcccagt
     8641 gtattgaatt ggtggtagaa ggtagttcca tgttccattt gtagatcttt aagattttat
     8701 ctttgataac tttaatagaa tgtggctcag ttctggtcct tcaagcctgt atggtttgga
     8761 ttttcagtag gggacagttg atgtggagtc aatctctttg gtacacagga agctttataa
     8821 aatttcattc acgaatctct tattttggga agctgttttg catatgagaa gaacactgtt
     8881 gaaataagga actaaagctt tatatattga tcaaggtgat tctgaaagtt ttaattttta
     8941 atgttgtaat gttatgttat tgttaattgt actttattat gtattcaata gaaaatcatg
     9001 atttattaat aaaagcttaa attctcatct a
//
