LOCUS       NM_006585               1854 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens chaperonin containing TCP1 subunit 8 (CCT8),
            transcript variant 1, mRNA.
ACCESSION   NM_006585 XM_375936
VERSION     NM_006585.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1854)
  AUTHORS   Gestaut,D., Roh,S.H., Ma,B., Pintilie,G., Joachimiak,L.A.,
            Leitner,A., Walzthoeni,T., Aebersold,R., Chiu,W. and Frydman,J.
  TITLE     The Chaperonin TRiC/CCT Associates with Prefoldin through a
            Conserved Electrostatic Interface Essential for Cellular
            Proteostasis
  JOURNAL   Cell 177 (3), 751-765 (2019)
   PUBMED   30955883
REFERENCE   4  (bases 1 to 1854)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   5  (bases 1 to 1854)
  AUTHORS   Yang,X., Ren,H., Shao,Y., Sun,Y., Zhang,L., Li,H., Zhang,X.,
            Yang,X., Yu,W. and Fu,J.
  TITLE     Chaperonin-containing T-complex protein 1 subunit 8 promotes cell
            migration and invasion in human esophageal squamous cell carcinoma
            by regulating alpha-actin and beta-tubulin expression
  JOURNAL   Int J Oncol 52 (6), 2021-2030 (2018)
   PUBMED   29620162
  REMARK    GeneRIF: Study demonstrated that CCT8 expression is widely
            upregulated in esophageal squamous cell carcinoma (ESCC), and
            enhanced the migration and invasion of ESCC cells, indicating that
            ectopic CCT8 expression contributes to the highly aggressive nature
            of ESCC probably by increasing alpha-actin and beta-tubulin
            expression.
REFERENCE   6  (bases 1 to 1854)
  AUTHORS   Yokota,S., Yanagi,H., Yura,T. and Kubota,H.
  TITLE     Cytosolic chaperonin-containing t-complex polypeptide 1 changes the
            content of a particular subunit species concomitant with substrate
            binding and folding activities during the cell cycle
  JOURNAL   Eur J Biochem 268 (17), 4664-4673 (2001)
   PUBMED   11532003
REFERENCE   7  (bases 1 to 1854)
  AUTHORS   Hynes,G.M. and Willison,K.R.
  TITLE     Individual subunits of the eukaryotic cytosolic chaperonin mediate
            interactions with binding sites located on subdomains of beta-actin
  JOURNAL   J Biol Chem 275 (25), 18985-18994 (2000)
   PUBMED   10748209
REFERENCE   8  (bases 1 to 1854)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
REFERENCE   9  (bases 1 to 1854)
  AUTHORS   Yamazaki,M., Ono,A., Watanabe,K., Sasaki,K., Tashiro,H. and
            Nomura,T.
  TITLE     Nucleotide sequence surrounding the locus marker D21S246 on human
            chromosome 21
  JOURNAL   DNA Res 2 (4), 187-189 (1995)
   PUBMED   8590283
REFERENCE   10 (bases 1 to 1854)
  AUTHORS   Kubota,H., Hynes,G. and Willison,K.
  TITLE     The eighth Cct gene, Cctq, encoding the theta subunit of the
            cytosolic chaperonin containing TCP-1
  JOURNAL   Gene 154 (2), 231-236 (1995)
   PUBMED   7890169
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC072001.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006585.3.
            
            Summary: This gene encodes the theta subunit of the CCT chaperonin,
            which is abundant in the eukaryotic cytosol and may be involved in
            the transport and assembly of newly synthesized proteins.
            Alternative splicing results in multiple transcript variants of
            this gene. A pseudogene related to this gene is located on
            chromosome 1. [provided by RefSeq, Sep 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC072001.1, SRR1660807.58830.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000286788.9/ ENSP00000286788.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1854              BC072001.1         8-1861
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q21.3"
     gene            1..1854
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /note="chaperonin containing TCP1 subunit 8"
                     /db_xref="GeneID:10694"
                     /db_xref="HGNC:HGNC:1623"
                     /db_xref="MIM:617786"
     exon            1..118
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1705
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /note="isoform 1 is encoded by transcript variant 1;
                     T-complex protein 1 subunit theta; CCT-theta; TCP-1-theta;
                     renal carcinoma antigen NY-REN-15; chaperonin containing
                     TCP1, subunit 8 (theta); chaperonin containing T-complex
                     polypeptide 1 subunit 8"
                     /codon_start=1
                     /product="T-complex protein 1 subunit theta isoform 1"
                     /protein_id="NP_006576.2"
                     /db_xref="CCDS:CCDS33528.1"
                     /db_xref="GeneID:10694"
                     /db_xref="HGNC:HGNC:1623"
                     /db_xref="MIM:617786"
                     /translation="MALHVPKAPGFAQMLKEGAKHFSGLEEAVYRNIQACKELAQTTR
                     TAYGPNGMNKMVINHLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVGDGTN
                     FVLVFAGALLELAEELLRIGLSVSEVIEGYEIACRKAHEILPNLVCCSAKNLRDIDEV
                     SSLLRTSIMSKQYGNEVFLAKLIAQACVSIFPDSGHFNVDNIRVCKILGSGISSSSVL
                     HGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLM
                     DAQVKAIADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALP
                     RLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAV
                     DDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPR
                     ALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAI
                     KLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND"
     exon            119..209
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            210..289
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            290..439
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            440..620
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            621..682
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            683..820
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            821..999
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1066
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1154
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1270
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1342
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1507
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1627
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1854
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1831..1836
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /note="hexamer: AATAAA"
     polyA_site      1854
                     /gene="CCT8"
                     /gene_synonym="C21orf112; Cctq; D21S246; PRED71"
                     /note="major polyA site"
ORIGIN      
        1 cctcgcttcc tccgcggtct tccgagcggt cgcgtgaact gcttcctgca ggctggccat
       61 ggcgcttcac gttcccaagg ctccgggctt tgcccagatg ctcaaggagg gagcgaaaca
      121 cttttcagga ttagaagagg ctgtgtatag aaacatacaa gcttgcaagg agcttgccca
      181 aaccactcgt acagcatatg gaccaaatgg aatgaacaaa atggttatca accacttgga
      241 gaagttgttt gtgacaaacg atgcagcaac tattttaaga gaactagaag tacagcatcc
      301 tgctgcaaaa atgattgtaa tggcttctca tatgcaagag caagaagttg gagatggcac
      361 aaactttgtt ctggtatttg ctggagctct cctggaatta gctgaagaac ttctgaggat
      421 tggcctgtca gtttcagagg tcatagaagg ttatgaaata gcctgcagaa aagctcatga
      481 gattcttcct aatttggtat gttgttctgc aaaaaacctt cgagatattg atgaagtctc
      541 atctctactt cgtacctcca taatgagtaa acaatatggt aatgaagtat ttctggccaa
      601 gcttattgct caggcatgcg tatctatttt tcctgattcc ggccatttca atgttgataa
      661 catcagagtt tgtaaaattc tgggctctgg tatcagttcc tcttcagtat tgcatggcat
      721 ggtttttaag aaggaaaccg aaggtgatgt aacatctgtc aaagatgcaa aaatagcagt
      781 gtactcttgt ccttttgatg gcatgataac agaaactaag ggaacagtgt tgataaagac
      841 tgctgaagaa ttgatgaatt ttagtaaggg agaagaaaac ctcatggatg cacaagtcaa
      901 agctattgct gatactggtg caaatgtcgt agtaacaggt ggcaaagtgg cagacatggc
      961 tcttcattat gcaaataaat ataatatcat gttagtgagg ctaaactcaa aatgggatct
     1021 ccgaagactt tgtaaaactg ttggtgctac agctcttcct agattgacac ctcctgtcct
     1081 tgaagaaatg ggacactgtg acagtgttta cctctcagaa gttggagata ctcaggtggt
     1141 ggtttttaag catgaaaagg aagatggcgc catttctacc atagtacttc gaggctctac
     1201 agacaatctg atggatgaca tagaaagggc agtagacgat ggtgttaata ctttcaaagt
     1261 tcttacaagg gataaacgtc ttgtacccgg aggtggagca acagaaattg aattagccaa
     1321 acagatcaca tcatatggag agacatgtcc tggacttgaa cagtatgcta ttaagaagtt
     1381 tgctgaggca tttgaagcta ttccccgcgc actggcagaa aactctggag ttaaggccaa
     1441 tgaagtaatc tctaaacttt atgcagtaca tcaagaagga aataaaaacg ttggattaga
     1501 tattgaggct gaagtccctg ctgtaaagga catgctggaa gctggtattc tagatactta
     1561 cctgggaaaa tattgggcta tcaaactcgc tactaatgct gcagtcactg tacttagagt
     1621 ggatcagatc atcatggcaa aaccagctgg tgggcccaag cctccaagtg ggaagaaaga
     1681 ctgggatgat gaccaaaatg attgaaattg gcttaatttt tactgtaggt gaaggctgta
     1741 tttgtagtag tactcaagaa tcacctgatg ttttcttatt ctccttaaat taagagttat
     1801 tttgtgtttg tattcttggc tggatgttat aataaacata ttgttactgt caaa
//
