LOCUS       NM_006584               1847 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens chaperonin containing TCP1 subunit 6B (CCT6B),
            transcript variant 1, mRNA.
ACCESSION   NM_006584
VERSION     NM_006584.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Gestaut,D., Roh,S.H., Ma,B., Pintilie,G., Joachimiak,L.A.,
            Leitner,A., Walzthoeni,T., Aebersold,R., Chiu,W. and Frydman,J.
  TITLE     The Chaperonin TRiC/CCT Associates with Prefoldin through a
            Conserved Electrostatic Interface Essential for Cellular
            Proteostasis
  JOURNAL   Cell 177 (3), 751-765 (2019)
   PUBMED   30955883
REFERENCE   3  (bases 1 to 1847)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 1847)
  AUTHORS   Love,C., Sun,Z., Jima,D., Li,G., Zhang,J., Miles,R., Richards,K.L.,
            Dunphy,C.H., Choi,W.W., Srivastava,G., Lugar,P.L., Rizzieri,D.A.,
            Lagoo,A.S., Bernal-Mizrachi,L., Mann,K.P., Flowers,C.R.,
            Naresh,K.N., Evens,A.M., Chadburn,A., Gordon,L.I., Czader,M.B.,
            Gill,J.I., Hsi,E.D., Greenough,A., Moffitt,A.B., McKinney,M.,
            Banerjee,A., Grubor,V., Levy,S., Dunson,D.B. and Dave,S.S.
  TITLE     The genetic landscape of mutations in Burkitt lymphoma
  JOURNAL   Nat Genet 44 (12), 1321-1325 (2012)
   PUBMED   23143597
REFERENCE   5  (bases 1 to 1847)
  AUTHORS   Mukherjee,K., Conway de Macario,E., Macario,A.J. and Brocchieri,L.
  TITLE     Chaperonin genes on the rise: new divergent classes and intense
            duplication in human and other vertebrate genomes
  JOURNAL   BMC Evol Biol 10, 64 (2010)
   PUBMED   20193073
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1847)
  AUTHORS   Stirling,P.C., Cuellar,J., Alfaro,G.A., El Khadali,F., Beh,C.T.,
            Valpuesta,J.M., Melki,R. and Leroux,M.R.
  TITLE     PhLP3 modulates CCT-mediated actin and tubulin folding via ternary
            complexes with substrates
  JOURNAL   J Biol Chem 281 (11), 7012-7021 (2006)
   PUBMED   16415341
  REMARK    GeneRIF: antagonistic actions of PhLP3 and prefoldin serve to
            modulate CCT activity and play a key role in establishing a
            functional cytoskeleton in vivo
REFERENCE   7  (bases 1 to 1847)
  AUTHORS   Kubota,H., Hynes,G.M., Kerr,S.M. and Willison,K.R.
  TITLE     Tissue-specific subunit of the mouse cytosolic
            chaperonin-containing TCP-1
  JOURNAL   FEBS Lett 402 (1), 53-56 (1997)
   PUBMED   9013858
  REMARK    GeneRIF: Describes the cloning of a very similar protein in mouse.
REFERENCE   8  (bases 1 to 1847)
  AUTHORS   Ozaki,K., Kuroki,T., Hayashi,S. and Nakamura,Y.
  TITLE     Isolation of three testis-specific genes (TSA303, TSA806, TSA903)
            by a differential mRNA display method
  JOURNAL   Genomics 36 (2), 316-319 (1996)
   PUBMED   8812458
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB032371.1, BC026125.2 and
            AK301773.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006584.3.
            
            Summary: This gene encodes a molecular chaperone that is a member
            of the chaperonin-containing TCP1 complex (CCT), also known as the
            TCP1 ring complex (TRiC). This complex consists of two identical
            stacked rings, each containing eight different proteins. Unfolded
            polypeptides enter the central cavity of the complex and are folded
            in an ATP-dependent manner. The complex folds various proteins,
            including actin and tubulin. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Feb 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.157746.1,
                                           SRR5189667.299527.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314144.10/ ENSP00000327191.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-110               DB032371.1         50-159
            111-272             DB032371.1         161-322
            273-1776            BC026125.2         215-1718
            1777-1847           AK301773.1         1604-1674
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..1847
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /note="chaperonin containing TCP1 subunit 6B"
                     /db_xref="GeneID:10693"
                     /db_xref="HGNC:HGNC:1621"
                     /db_xref="MIM:610730"
     exon            1..204
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..1660
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /note="isoform 1 is encoded by transcript variant 1;
                     testis-specific Tcp20; testis-specific protein TSA303;
                     chaperonin-containing T-complex polypeptide 1, subunit 6B;
                     T-complex protein 1 subunit zeta-2; chaperonin containing
                     TCP1, subunit 6B (zeta 2)"
                     /codon_start=1
                     /product="T-complex protein 1 subunit zeta-2 isoform 1"
                     /protein_id="NP_006575.2"
                     /db_xref="CCDS:CCDS32617.1"
                     /db_xref="GeneID:10693"
                     /db_xref="HGNC:HGNC:1621"
                     /db_xref="MIM:610730"
                     /translation="MAAIKAVNSKAEVARARAALAVNICAARGLQDVLRTNLGPKGTM
                     KMLVSGAGDIKLTKDGNVLLDEMQIQHPTASLIAKVATAQDDVTGDGTTSNVLIIGEL
                     LKQADLYISEGLHPRIIAEGFEAAKIKALEVLEEVKVTKEMKRKILLDVARTSLQTKV
                     HAELADVLTEVVVDSVLAVRRPGYPIDLFMVEIMEMKHKLGTDTKLIQGLVLDHGARH
                     PDMKKRVEDAFILICNVSLEYEKTEVNSGFFYKTAEEKEKLVKAERKFIEDRVQKIID
                     LKDKVCAQSNKGFVVINQKGIDPFSLDSLAKHGIVALRRAKRRNMERLSLACGGMAVN
                     SFEDLTVDCLGHAGLVYEYTLGEEKFTFIEECVNPCSVTLLVKGPNKHTLTQVKDAIR
                     DGLRAIKNAIEDGCMVPGAGAIEVAMAEALVTYKNSIKGRARLGVQAFADALLIIPKV
                     LAQNAGYDPQETLVKVQAEHVESKQLVGVDLNTGEPMVAADAGVWDNYCVKKQLLHSC
                     TVIATNILLVDEIMRAGMSSLK"
     exon            205..268
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            269..403
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            404..577
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            578..681
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            682..792
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            793..952
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1035
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1132
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1280
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1414
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1517
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1590
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1847
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1758..1763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /note="hexamer: AATAAA"
     polyA_site      1789
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /note="major polyA site"
     regulatory      1827..1832
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
                     /note="hexamer: AATAAA"
     polyA_site      1847
                     /gene="CCT6B"
                     /gene_synonym="CCT-zeta-2; CCTZ-2; Cctz2; TCP-1-zeta-2;
                     TSA303"
ORIGIN      
        1 gcattgccgt ttttgctcag aatcgcgact aaggcttttt ttttttctcc ctctgaacgg
       61 ttaggctatg gctgcgataa aggccgtcaa ctccaaggct gaggtggcgc gggcccgggc
      121 agctttggct gtcaatatat gcgccgcccg agggctgcag gatgtgctgc ggaccaactt
      181 gggtcctaaa ggcaccatga aaatgcttgt ttctggtgca ggtgacatca aactcaccaa
      241 agatggcaat gtgctgctcg atgagatgca aattcaacat ccaacagctt ccttgatagc
      301 aaaagtagca acagctcagg atgacgtcac aggagatggt actacttcaa atgttctaat
      361 tattggagag ttattaaaac aagctgacct gtacatttct gagggcctgc accctagaat
      421 aatagctgaa ggatttgaag ctgcaaagat aaaagcactt gaagttttgg aggaagttaa
      481 agtgacaaag gagatgaaaa gaaaaatcct cttagatgta gctagaacat cattacaaac
      541 taaagttcat gctgaactgg ctgatgtctt aacagaggtt gtggtggatt ctgttttggc
      601 tgttagaaga ccaggttacc ctattgatct cttcatggta gaaataatgg agatgaagca
      661 taaattagga acagatacaa agttgatcca aggattagtt ttggatcatg gtgcccgtca
      721 tccagatatg aagaagcgag tagaagatgc atttatcctt atttgcaacg tttcactgga
      781 atatgaaaaa acagaggtga actctggttt cttttataag actgcagaag agaaagagaa
      841 attggtaaaa gctgaaagaa aatttattga agatagagta caaaaaataa tagacctgaa
      901 ggacaaagtc tgtgctcagt caaataaagg atttgtcgtc attaatcaaa agggaattga
      961 tccattttcc ttagattctc ttgcaaaaca tggaatagta gctcttcgca gagcaaaaag
     1021 aagaaatatg gaaagactct ctcttgcttg tggtggaatg gccgtgaatt cttttgaaga
     1081 tctcactgta gattgcttgg gacatgctgg tcttgtgtat gagtatacat taggtgaaga
     1141 aaagttcact tttattgagg agtgtgttaa cccttgctct gttaccttgt tggttaaagg
     1201 accaaataag catactctca cacaagtcaa ggatgccata agagatggac ttcgtgctat
     1261 caaaaatgcc attgaagatg gttgtatggt tcctggagct ggtgcaattg aagtggcaat
     1321 ggctgaagct cttgttacat ataagaacag tataaaagga agagctcgtc ttggagtcca
     1381 agcttttgct gatgccttac tcattattcc caaggttctt gctcagaatg ctggttatga
     1441 cccacaggaa acattagtaa aagttcaggc tgagcatgtc gagtcaaaac aacttgtggg
     1501 cgtagatttg aatacaggtg agccaatggt agcagcagat gcaggagttt gggataatta
     1561 ttgtgtaaaa aaacaacttc ttcactcttg cacagtgatt gccaccaaca ttctcctggt
     1621 tgatgaaatt atgcgagctg ggatgtcttc tctcaaatga tgattgaatt caaaatcaac
     1681 ccttctagaa gatgaaattt agtacacttt acatctgact actattgtgt agcctgagcc
     1741 attctgaatt tctacacaat aaatgcagtt tatgtctttt gggtcttaat agtctcaaaa
     1801 atatttcatc aaggtataaa gaacacaata aatactttta cagtaaa
//
