LOCUS       NM_012073               3293 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens chaperonin containing TCP1 subunit 5 (CCT5),
            transcript variant 1, mRNA.
ACCESSION   NM_012073
VERSION     NM_012073.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wu,S. and Peng,L.
  TITLE     Increased CCT5 expression is a potential unfavourable factor
            promoting the growth of nasopharyngeal carcinoma
  JOURNAL   J Int Med Res 52 (9), 3000605241271754 (2024)
   PUBMED   39286844
  REMARK    GeneRIF: Increased CCT5 expression is a potential unfavourable
            factor promoting the growth of nasopharyngeal carcinoma.
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Scalia,F., Lo Bosco,G., Paladino,L., Vitale,A.M., Noori,L., Conway
            de Macario,E., Macario,A.J.L., Bucchieri,F., Cappello,F. and Lo
            Celso,F.
  TITLE     Structural and Dynamic Disturbances Revealed by Molecular Dynamics
            Simulations Predict the Impact on Function of CCT5 Chaperonin
            Mutations Associated with Rare Severe Distal Neuropathies
  JOURNAL   Int J Mol Sci 24 (3), 2018 (2023)
   PUBMED   36768350
  REMARK    GeneRIF: Structural and Dynamic Disturbances Revealed by Molecular
            Dynamics Simulations Predict the Impact on Function of CCT5
            Chaperonin Mutations Associated with Rare Severe Distal
            Neuropathies.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3293)
  AUTHORS   Li,Y., Liu,C., Zhang,X., Huang,X., Liang,S., Xing,F. and Tian,H.
  TITLE     CCT5 induces epithelial-mesenchymal transition to promote gastric
            cancer lymph node metastasis by activating the Wnt/beta-catenin
            signalling pathway
  JOURNAL   Br J Cancer 126 (12), 1684-1694 (2022)
   PUBMED   35194191
  REMARK    GeneRIF: CCT5 induces epithelial-mesenchymal transition to promote
            gastric cancer lymph node metastasis by activating the
            Wnt/beta-catenin signalling pathway.
REFERENCE   4  (bases 1 to 3293)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 3293)
  AUTHORS   Sergeeva,O.A., Tran,M.T., Haase-Pettingell,C. and King,J.A.
  TITLE     Biochemical characterization of mutants in chaperonin proteins CCT4
            and CCT5 associated with hereditary sensory neuropathy
  JOURNAL   J Biol Chem 289 (40), 27470-27480 (2014)
   PUBMED   25124038
  REMARK    GeneRIF: H147R CCT5 was not as efficient in chaperoning these
            substrates as wild type CCT5.
REFERENCE   6  (bases 1 to 3293)
  AUTHORS   Bouhouche,A., Benomar,A., Bouslam,N., Chkili,T. and Yahyaoui,M.
  TITLE     Mutation in the epsilon subunit of the cytosolic
            chaperonin-containing t-complex peptide-1 (Cct5) gene causes
            autosomal recessive mutilating sensory neuropathy with spastic
            paraplegia
  JOURNAL   J Med Genet 43 (5), 441-443 (2006)
   PUBMED   16399879
  REMARK    GeneRIF: A missense mutation within the CCT5 gene is associated
            with autosomal recessive mutilating sensory neuropathy with spastic
            paraplegia.
REFERENCE   7  (bases 1 to 3293)
  AUTHORS   Roperch,J.P., Lethrone,F., Prieur,S., Piouffre,L., Israeli,D.,
            Tuynder,M., Nemani,M., Pasturaud,P., Gendron,M.C., Dausset,J.,
            Oren,M., Amson,R.B. and Telerman,A.
  TITLE     SIAH-1 promotes apoptosis and tumor suppression through a network
            involving the regulation of protein folding, unfolding, and
            trafficking: identification of common effectors with p53 and
            p21(Waf1)
  JOURNAL   Proc Natl Acad Sci U S A 96 (14), 8070-8073 (1999)
   PUBMED   10393949
REFERENCE   8  (bases 1 to 3293)
  AUTHORS   Liou,A.K. and Willison,K.R.
  TITLE     Elucidation of the subunit orientation in CCT (chaperonin
            containing TCP1) from the subunit composition of CCT
            micro-complexes
  JOURNAL   EMBO J 16 (14), 4311-4316 (1997)
   PUBMED   9250675
REFERENCE   9  (bases 1 to 3293)
  AUTHORS   Roobol,A., Holmes,F.E., Hayes,N.V., Baines,A.J. and Carden,M.J.
  TITLE     Cytoplasmic chaperonin complexes enter neurites developing in vitro
            and differ in subunit composition within single cells
  JOURNAL   J Cell Sci 108 (Pt 4), 1477-1488 (1995)
   PUBMED   7615668
REFERENCE   10 (bases 1 to 3293)
  AUTHORS   Kubota,H., Hynes,G., Carne,A., Ashworth,A. and Willison,K.
  TITLE     Identification of six Tcp-1-related genes encoding divergent
            subunits of the TCP-1-containing chaperonin
  JOURNAL   Curr Biol 4 (2), 89-99 (1994)
   PUBMED   7953530
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from FJ224303.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012073.4.
            
            Summary: The protein encoded by this gene is a molecular chaperone
            that is a member of the chaperonin containing TCP1 complex (CCT),
            also known as the TCP1 ring complex (TRiC). This complex consists
            of two identical stacked rings, each containing eight different
            proteins. Unfolded polypeptides enter the central cavity of the
            complex and are folded in an ATP-dependent manner. The complex
            folds various proteins, including actin and tubulin. Mutations in
            this gene cause hereditary sensory and autonomic neuropathy with
            spastic paraplegia (HSNSP). Alternative splicing results in
            multiple transcript variants. Related pseudogenes have been
            identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FJ224303.1, SRR1803615.113869.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000280326.9/ ENSP00000280326.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3293              FJ224303.1         111-3403
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.2"
     gene            1..3293
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="chaperonin containing TCP1 subunit 5"
                     /db_xref="GeneID:22948"
                     /db_xref="HGNC:HGNC:1618"
                     /db_xref="MIM:610150"
     exon            1..166
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1687
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="isoform a is encoded by transcript variant 1;
                     T-complex protein 1 subunit epsilon; epididymis secretory
                     protein Li 69; chaperonin containing TCP1, subunit 5
                     (epsilon); chaperonin containing T-complex polypeptide 1
                     subunit 5"
                     /codon_start=1
                     /product="T-complex protein 1 subunit epsilon isoform a"
                     /protein_id="NP_036205.1"
                     /db_xref="CCDS:CCDS3877.1"
                     /db_xref="GeneID:22948"
                     /db_xref="HGNC:HGNC:1618"
                     /db_xref="MIM:610150"
                     /translation="MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAV
                     ANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEI
                     GDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIK
                     DTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGR
                     LEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKA
                     LQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIA
                     IATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNK
                     MIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRA
                     FADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIE
                     TLIGKKQQISLATQMVRMILKIDDIRKPGESEE"
     exon            167..227
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            228..392
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            393..591
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            592..784
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            785..934
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1054
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1240
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1378
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1559
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..3293
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1895..1900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="hexamer: ATTAAA"
     polyA_site      1917
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="major polyA site"
     regulatory      1963..1968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="hexamer: ATTAAA"
     polyA_site      1984
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="major polyA site"
     regulatory      3274..3279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
                     /note="hexamer: AATAAA"
     polyA_site      3293
                     /gene="CCT5"
                     /gene_synonym="CCT-epsilon; CCTE; HEL-S-69; HSNSP;
                     PNAS-102; TCP-1-epsilon"
ORIGIN      
        1 gcattctcgc ttccgtagcg gtctccgccg gttgggggga agtaattccg gttgttgcac
       61 catggcgtcc atggggaccc tcgccttcga tgaatatggg cgccctttcc tcatcatcaa
      121 ggatcaggac cgcaagtccc gtcttatggg acttgaggcc ctcaagtctc atataatggc
      181 agcaaaggct gtagcaaata caatgagaac atcacttgga ccaaatgggc ttgataagat
      241 gatggtggat aaggatggag atgtgactgt aactaatgat ggggccacca tcttaagcat
      301 gatggatgtt gatcatcaga ttgccaagct gatggtggaa ctgtccaagt ctcaggatga
      361 tgaaattgga gatggaacca caggagtggt tgtcctggct ggtgccttgt tagaagaagc
      421 ggagcaattg ctagaccgag gcattcaccc aatcagaata gccgatggct atgagcaggc
      481 tgctcgtgtt gctattgaac acctggacaa gatcagcgat agcgtccttg ttgacataaa
      541 ggacaccgaa cccctgattc agacagcaaa aaccacgctg ggctccaaag tggtcaacag
      601 ttgtcaccga cagatggctg agattgctgt gaatgccgtc ctcactgtag cagatatgga
      661 gcggagagac gttgactttg agcttatcaa agtagaaggc aaagtgggcg gcaggctgga
      721 ggacactaaa ctgattaagg gcgtgattgt ggacaaggat ttcagtcacc cacagatgcc
      781 aaaaaaagtg gaagatgcga agattgcaat tctcacatgt ccatttgaac cacccaaacc
      841 aaaaacaaag cataagctgg atgtgacctc tgtcgaagat tataaagccc ttcagaaata
      901 cgaaaaggag aaatttgaag agatgattca acaaattaaa gagactggtg ctaacctagc
      961 aatttgtcag tggggctttg atgatgaagc aaatcactta cttcttcaga acaacttgcc
     1021 tgcggttcgc tgggtaggag gacctgaaat tgagctgatt gccatcgcaa caggagggcg
     1081 gatcgtcccc aggttctcag agctcacagc cgagaagctg ggctttgctg gtcttgtaca
     1141 ggagatctca tttgggacaa ctaaggataa aatgctggtc atcgagcagt gtaagaactc
     1201 cagagctgta accattttta ttagaggagg aaataagatg atcattgagg aggcgaaacg
     1261 atcccttcac gatgctttgt gtgtcatccg gaacctcatc cgcgataatc gtgtggtgta
     1321 tggaggaggg gctgctgaga tatcctgtgc cctggcagtt agccaagagg cggataagtg
     1381 ccccacctta gaacagtatg ccatgagagc gtttgccgac gcactggagg tcatccccat
     1441 ggccctctct gaaaacagtg gcatgaatcc catccagact atgaccgaag tccgagccag
     1501 acaggtgaag gagatgaacc ctgctcttgg catcgactgt ttgcacaagg ggacaaatga
     1561 tatgaagcaa cagcatgtca tagaaacctt gattggcaaa aagcaacaga tatctcttgc
     1621 aacacaaatg gttagaatga ttttgaagat tgatgacatt cgtaagcctg gagaatctga
     1681 agaatgaaga cattgagaaa actatgtagc aagatccact tctgtgatta agtaaatgga
     1741 tgtctcgtga tgcatctaca gttatttatt gttacatcct tttccagaca ctgtagatgc
     1801 tataataaaa atagctgttt ggtaaccata gtttcacttg ttcaaagctg tgtaatcgtg
     1861 ggggtaccat ctcaactgct tttgtattca ttgtattaaa agaatctgtt taaacaacct
     1921 ttatcttctc ttcgggttta agaaacgttt attgtaacag taattaaatg ctgccttaat
     1981 tgaaggggtt tgggtggatt tttttttctc aaaataagct gtagggacta ttttaacagc
     2041 ttaaacagga gctctcaaga tgcactttca tattgagagg aatatgggct tgatcctctt
     2101 cctatctaaa tgggtgggcc atttgattgt agagggtcca ccacagaatt atgggatgcc
     2161 ttaagtgctg ttactaggtt gctcacagcc taacctggcg tgttgtttag ggctgatgga
     2221 gacccatgtg agcctttgct ttcctctggc cccggcccca ccctgaacac agctcataca
     2281 cagaatcagg accagcatgt gcagagctgg ccaccagcac aggcttaggg cagttcagaa
     2341 cccacttgtt tccctatcag agggacacag tgaagtggag gttaaagtaa attacaggaa
     2401 ataagggaga aatcttgcag ttaccatgtt cagatagagt gactgaaatt aattgtactt
     2461 actaaagtat taactagcta acagtgatgg gccaagacgc tccgagaact ctaccgggat
     2521 tgtctgttct gacaacccag tgaggcagat acactttctt actgctcaca tcttacaggt
     2581 gagtactcat aattggccag catctcacca ccagcaagta gtagggccag gtcaatccca
     2641 ggcagtctga ccccagagtg gcccagctca tcccctactc tgttatttgc ttgttaatga
     2701 ttctctagat tttctaaaat aatgtttctt agcattgtga tgataaagct catgatgaac
     2761 tttatcacta gttatgccac cttaactagt cagatttcct agaattagga aatggtgact
     2821 cttgtctaaa tttggttaag tgatgaattt gggttaccgt ctcatgtgaa cctggagatt
     2881 caccagtctt aacttttggg tcattgtgtt ttctctacat tcatgcattg gatgttttgc
     2941 taaataactc ctgtggattt aggaatgtgt gctaatagca atcttcctaa ttttcatgtt
     3001 tatatggaac tatgcagttg agtattgaaa gctttaaact gagtttattt acaaggactg
     3061 agtctagcct acagagaaca tacagcagcc ttctttggac cacagtctta tccgaggggt
     3121 ctgtggttgt atcagaagag ccactaaacc aatccccctt tccaaaattg aacctcacag
     3181 acgttcctgt tttttgtgat tgagaaactg gtcaatgaac aggaagactt agagatgttt
     3241 cacaagcctt tgatttttgt ttcatttatt tgtaataaac ctcttccacg tga
//
