LOCUS       NM_006431               1916 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens chaperonin containing TCP1 subunit 2 (CCT2),
            transcript variant 1, mRNA.
ACCESSION   NM_006431
VERSION     NM_006431.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Liu,W., Lin,R., Zhu,C., Chen,Y., Gao,Q. and Zhong,J.
  TITLE     CCT2 Regulates ZEB1-Induced EMT Gene Transcription to Promote the
            Metastasis and Tumorigenesis of Papillary Thyroid Carcinoma
  JOURNAL   Discov Med 36 (188), 1819-1830 (2024)
   PUBMED   39327245
  REMARK    GeneRIF: CCT2 Regulates ZEB1-Induced EMT Gene Transcription to
            Promote the Metastasis and Tumorigenesis of Papillary Thyroid
            Carcinoma.
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Chen,J., Hu,Q., Zhou,C. and Jin,D.
  TITLE     CCT2 prevented beta-catenin proteasomal degradation to sustain
            cancer stem cell traits and promote tumor progression in epithelial
            ovarian cancer
  JOURNAL   Mol Biol Rep 51 (1), 54 (2024)
   PUBMED   38165547
  REMARK    GeneRIF: CCT2 prevented beta-catenin proteasomal degradation to
            sustain cancer stem cell traits and promote tumor progression in
            epithelial ovarian cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1916)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 1916)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1916)
  AUTHORS   Okitsu,Y., Nagano,M., Yamagata,T., Ito,C., Toshimori,K., Dohra,H.,
            Fujii,W. and Yogo,K.
  TITLE     Dlec1 is required for spermatogenesis and male fertility in mice
  JOURNAL   Sci Rep 10 (1), 18883 (2020)
   PUBMED   33144677
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1916)
  AUTHORS   Yokota,S., Yanagi,H., Yura,T. and Kubota,H.
  TITLE     Cytosolic chaperonin-containing t-complex polypeptide 1 changes the
            content of a particular subunit species concomitant with substrate
            binding and folding activities during the cell cycle
  JOURNAL   Eur J Biochem 268 (17), 4664-4673 (2001)
   PUBMED   11532003
REFERENCE   7  (bases 1 to 1916)
  AUTHORS   Hynes,G.M. and Willison,K.R.
  TITLE     Individual subunits of the eukaryotic cytosolic chaperonin mediate
            interactions with binding sites located on subdomains of beta-actin
  JOURNAL   J Biol Chem 275 (25), 18985-18994 (2000)
   PUBMED   10748209
REFERENCE   8  (bases 1 to 1916)
  AUTHORS   Llorca,O., Smyth,M.G., Carrascosa,J.L., Willison,K.R.,
            Radermacher,M., Steinbacher,S. and Valpuesta,J.M.
  TITLE     3D reconstruction of the ATP-bound form of CCT reveals the
            asymmetric folding conformation of a type II chaperonin
  JOURNAL   Nat Struct Biol 6 (7), 639-642 (1999)
   PUBMED   10404219
REFERENCE   9  (bases 1 to 1916)
  AUTHORS   Won,K.A., Schumacher,R.J., Farr,G.W., Horwich,A.L. and Reed,S.I.
  TITLE     Maturation of human cyclin E requires the function of eukaryotic
            chaperonin CCT
  JOURNAL   Mol Cell Biol 18 (12), 7584-7589 (1998)
   PUBMED   9819444
REFERENCE   10 (bases 1 to 1916)
  AUTHORS   Kubota,H., Hynes,G., Carne,A., Ashworth,A. and Willison,K.
  TITLE     Identification of six Tcp-1-related genes encoding divergent
            subunits of the TCP-1-containing chaperonin
  JOURNAL   Curr Biol 4 (2), 89-99 (1994)
   PUBMED   7953530
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB138063.1, AF026166.1 and
            AA401491.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006431.2.
            
            Summary: The protein encoded by this gene is a molecular chaperone
            that is a member of the chaperonin containing TCP1 complex (CCT),
            also known as the TCP1 ring complex (TRiC). This complex consists
            of two identical stacked rings, each containing eight different
            proteins. Unfolded polypeptides enter the central cavity of the
            complex and are folded in an ATP-dependent manner. The complex
            folds various proteins, including actin and tubulin. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.241223.1,
                                           SRR3476690.731237.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299300.11/ ENSP00000299300.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 CB138063.1         37-42
            7-1868              AF026166.1         1-1862
            1869-1916           AA401491.1         16-63               c
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q15"
     gene            1..1916
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /note="chaperonin containing TCP1 subunit 2"
                     /db_xref="GeneID:10576"
                     /db_xref="HGNC:HGNC:1615"
                     /db_xref="MIM:605139"
     exon            1..66
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1671
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /note="isoform 1 is encoded by transcript variant 1;
                     chaperonin containing t-complex polypeptide 1, beta
                     subunit; chaperonin containing t-complex polypeptide 1,
                     subunit 2; T-complex protein 1, beta subunit; T-complex
                     protein 1 subunit beta; epididymis secretory sperm binding
                     protein Li 100n; chaperonin containing TCP1, subunit 2
                     (beta)"
                     /codon_start=1
                     /product="T-complex protein 1 subunit beta isoform 1"
                     /protein_id="NP_006422.1"
                     /db_xref="CCDS:CCDS8991.1"
                     /db_xref="GeneID:10576"
                     /db_xref="HGNC:HGNC:1615"
                     /db_xref="MIM:605139"
                     /translation="MASLSLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLG
                     PKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLVDMSRVQDDEVGDGTTSV
                     TVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFRQDLM
                     NIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGF
                     LLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMK
                     EKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIAST
                     FDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHD
                     ALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTII
                     ADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAA
                     EAAEVILRVDNIIKAAPRKRVPDHHPC"
     exon            67..141
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            142..207
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            208..319
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            320..396
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            397..509
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            510..712
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            713..813
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            814..941
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1045
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1165
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1294
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1398
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1498
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1640
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1916
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1897..1902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /note="hexamer: AATAAA"
     polyA_site      1916
                     /gene="CCT2"
                     /gene_synonym="99D8.1; CCT-beta; CCTB; HEL-S-100n;
                     PRO1633; TCP-1-beta"
                     /note="major polyA site"
ORIGIN      
        1 agtccgctgg tcccgagcac gagctgtgag gggattcact tgtgtgcgga actcctcgga
       61 accatggcgt ccctttccct tgcacctgtt aacatcttta aggcaggagc tgatgaagag
      121 agagcagaga cagctcgtct gacttctttt attggtgcca tcgccattgg agacttggta
      181 aagagcacct tgggacccaa aggcatggac aaaattcttc taagcagtgg acgagatgcc
      241 tctcttatgg taaccaatga tggtgccact attctaaaaa acattggtgt tgacaatcca
      301 gcagctaaag ttttagttga tatgtcaagg gttcaagatg atgaagttgg tgatggcact
      361 acctctgtta ccgttttagc agcagaatta ttaagggaag cagaatcttt aattgcaaaa
      421 aagattcatc cacagaccat catagcgggt tggagagaag ccacgaaggc tgcaagagag
      481 gcgctgttga gttctgcagt tgatcatggt tccgatgaag ttaaattccg tcaagattta
      541 atgaatattg cgggcacaac attatcctca aaacttctta ctcatcacaa agaccacttt
      601 acaaagttag ctgtagaagc agttctcaga ctgaaaggct ctggcaacct ggaggcaatt
      661 catattatca agaagctagg aggaagtttg gcagattcct atttagatga aggcttcctg
      721 ttggataaaa aaattggagt aaatcaacca aaacgaattg aaaatgctaa aattcttatt
      781 gcaaatactg gtatggatac agacaaaata aagatatttg gttcccgggt aagagttgac
      841 tctacagcaa aggttgcaga aatagaacat gcggaaaagg aaaaaatgaa ggagaaagtt
      901 gaacgtattc ttaagcatgg aataaattgc tttattaaca ggcaattaat ttataattat
      961 cctgaacagc tctttggtgc tgctggtgtc atggctattg agcatgcaga ttttgcaggt
     1021 gtggaacgcc tagctcttgt cacaggtggt gaaattgcct ctacctttga tcacccagaa
     1081 ctggtgaagc ttggaagttg caaacttatc gaggaagtca tgattggaga agacaaactc
     1141 attcactttt ctggggttgc ccttggtgag gcttgtacca ttgttttgcg tggtgccact
     1201 caacaaattt tagatgaagc agaaagatca ttgcatgatg ctctttgtgt tcttgcgcaa
     1261 actgtaaagg actctagaac agtttatgga ggaggctgtt ctgagatgtt gatggctcat
     1321 gctgtgacac agcttgccaa tagaacacca ggcaaagaag ctgttgcaat ggagtcttat
     1381 gctaaagcac tgagaatgtt gccaaccatc atagctgaca atgcaggcta tgacagtgca
     1441 gacctggtgg cacagctcag ggctgctcac agtgaaggca ataccactgc tggattggat
     1501 atgagggaag gcaccattgg agatatggct atcctgggta taacagaaag ttttcaagtg
     1561 aagcgacagg ttcttctgag tgcagctgaa gcagcagagg tgattctgcg tgtggacaac
     1621 atcatcaaag cggcacccag gaaacgtgtc cctgatcacc acccctgtta agcattccca
     1681 cgtgctgtcg atctttggac cagtttctag caaagttgtg tttgaaagat actctattaa
     1741 agaagactgt ggaatctgtt tatcggtgcc cattatatcc ttaagtttgg atatttagct
     1801 gaccttcgct ttaacatagg tctaatttat ttgccgtgtc attttccata caaatcagtt
     1861 gatttaaaaa agttcatttc tcatactgtg cattaaaata aaaatttgaa caatta
//
