LOCUS       NM_002984                660 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens C-C motif chemokine ligand 4 (CCL4), mRNA.
ACCESSION   NM_002984
VERSION     NM_002984.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Deng,H., Xue,P., Zhou,X., Wang,Y. and Liu,W.
  TITLE     CCL4/CCR5 regulates chondrocyte biology and OA progression
  JOURNAL   Cytokine 183, 156746 (2024)
   PUBMED   39236430
  REMARK    GeneRIF: CCL4/CCR5 regulates chondrocyte biology and OA
            progression.
REFERENCE   2  (bases 1 to 660)
  AUTHORS   Chang,T.T., Lin,L.Y., Chen,C. and Chen,J.W.
  TITLE     CCL4 contributes to aging related angiogenic insufficiency through
            activating oxidative stress and endothelial inflammation
  JOURNAL   Angiogenesis 27 (3), 475-499 (2024)
   PUBMED   38739303
  REMARK    GeneRIF: CCL4 contributes to aging related angiogenic insufficiency
            through activating oxidative stress and endothelial inflammation.
REFERENCE   3  (bases 1 to 660)
  AUTHORS   Gong,W., Zhou,J., Hou,Y., Zhang,J., He,P. and Yu,Q.
  TITLE     The causal relationship between immune cells mediating FIT3L, CCL4,
            OSM, and skin-derived deteriorated tumors
  JOURNAL   Skin Res Technol 30 (7), e13774 (2024)
   PUBMED   38953214
  REMARK    GeneRIF: The causal relationship between immune cells mediating
            FIT3L, CCL4, OSM, and skin-derived deteriorated tumors.
REFERENCE   4  (bases 1 to 660)
  AUTHORS   Xu,X., Zhao,Y., Ying,Y., Zhu,H., Luo,J., Mou,T. and Zhang,Z.
  TITLE     m7G-related genes-NCBP2 and EIF4E3 determine immune contexture in
            head and neck squamous cell carcinoma by regulating CCL4/CCL5
            expression
  JOURNAL   Mol Carcinog 62 (8), 1091-1106 (2023)
   PUBMED   37067401
  REMARK    GeneRIF: m7G-related genes-NCBP2 and EIF4E3 determine immune
            contexture in head and neck squamous cell carcinoma by regulating
            CCL4/CCL5 expression.
REFERENCE   5  (bases 1 to 660)
  AUTHORS   Chen,R., Ma,L., Jiang,C. and Zhang,S.
  TITLE     Expression and potential role of CCL4 in CD8+T cells in NSCLC
  JOURNAL   Clin Transl Oncol 24 (12), 2420-2431 (2022)
   PUBMED   35964269
  REMARK    GeneRIF: Expression and potential role of CCL4 in CD8+T cells in
            NSCLC.
REFERENCE   6  (bases 1 to 660)
  AUTHORS   Napolitano,M., Modi,W.S., Cevario,S.J., Gnarra,J.R., Seuanez,H.N.
            and Leonard,W.J.
  TITLE     The gene encoding the Act-2 cytokine. Genomic structure, HTLV-I/Tax
            responsiveness of 5' upstream sequences, and chromosomal
            localization
  JOURNAL   J Biol Chem 266 (26), 17531-17536 (1991)
   PUBMED   1894635
REFERENCE   7  (bases 1 to 660)
  AUTHORS   Baixeras,E., Roman-Roman,S., Jitsukawa,S., Genevee,C., Mechiche,S.,
            Viegas-Pequignot,E., Hercend,T. and Triebel,F.
  TITLE     Cloning and expression of a lymphocyte activation gene (LAG-1)
  JOURNAL   Mol Immunol 27 (11), 1091-1102 (1990)
   PUBMED   2247088
REFERENCE   8  (bases 1 to 660)
  AUTHORS   Irving,S.G., Zipfel,P.F., Balke,J., McBride,O.W., Morton,C.C.,
            Burd,P.R., Siebenlist,U. and Kelly,K.
  TITLE     Two inflammatory mediator cytokine genes are closely linked and
            variably amplified on chromosome 17q
  JOURNAL   Nucleic Acids Res 18 (11), 3261-3270 (1990)
   PUBMED   1972563
REFERENCE   9  (bases 1 to 660)
  AUTHORS   Brown,K.D., Zurawski,S.M., Mosmann,T.R. and Zurawski,G.
  TITLE     A family of small inducible proteins secreted by leukocytes are
            members of a new superfamily that includes leukocyte and
            fibroblast-derived inflammatory agents, growth factors, and
            indicators of various activation processes
  JOURNAL   J Immunol 142 (2), 679-687 (1989)
   PUBMED   2521353
REFERENCE   10 (bases 1 to 660)
  AUTHORS   Lipes,M.A., Napolitano,M., Jeang,K.T., Chang,N.T. and Leonard,W.J.
  TITLE     Identification, cloning, and characterization of an immune
            activation gene
  JOURNAL   Proc Natl Acad Sci U S A 85 (24), 9704-9708 (1988)
   PUBMED   2462251
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY766446.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002984.3.
            
            Summary: The protein encoded by this gene is a mitogen-inducible
            monokine and is one of the major HIV-suppressive factors produced
            by CD8+ T-cells. The encoded protein is secreted and has
            chemokinetic and inflammatory functions. [provided by RefSeq, Dec
            2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY766446.1, SRR5189652.231329.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000615863.2/ ENSP00000482259.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-660               AY766446.1         1-660
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..660
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /note="C-C motif chemokine ligand 4"
                     /db_xref="GeneID:6351"
                     /db_xref="HGNC:HGNC:10630"
                     /db_xref="MIM:182284"
     exon            1..155
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..358
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /note="small inducible cytokine A4 (homologous to mouse
                     Mip-1b); secreted protein G-26; PAT 744; SIS-gamma;
                     MIP-1-beta(1-69); T-cell activation protein 2; G-26
                     T-lymphocyte-secreted protein; lymphocyte activation gene
                     1 protein; macrophage inflammatory protein 1-beta;
                     chemokine (C-C motif) ligand 4"
                     /codon_start=1
                     /product="C-C motif chemokine 4 precursor"
                     /protein_id="NP_002975.1"
                     /db_xref="CCDS:CCDS11308.1"
                     /db_xref="GeneID:6351"
                     /db_xref="HGNC:HGNC:10630"
                     /db_xref="MIM:182284"
                     /translation="MKLCVTVLSLLMLVAAFCSPALSAPMGSDPPTACCFSYTARKLP
                     RNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN"
     exon            156..270
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /inference="alignment:Splign:2.1.0"
     exon            271..660
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      637..642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /note="hexamer: AATAAA"
     polyA_site      660
                     /gene="CCL4"
                     /gene_synonym="ACT2; AT744.1; G-26; HC21; LAG-1; LAG1;
                     MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4"
                     /note="major polyA site"
ORIGIN      
        1 agcacaggac acagctgggt tctgaagctt ctgagttctg cagcctcacc tctgagaaaa
       61 cctcttttcc accaatacca tgaagctctg cgtgactgtc ctgtctctcc tcatgctagt
      121 agctgccttc tgctctccag cgctctcagc accaatgggc tcagaccctc ccaccgcctg
      181 ctgcttttct tacaccgcga ggaagcttcc tcgcaacttt gtggtagatt actatgagac
      241 cagcagcctc tgctcccagc cagctgtggt attccaaacc aaaagaagca agcaagtctg
      301 tgctgatccc agtgaatcct gggtccagga gtacgtgtat gacctggaac tgaactgagc
      361 tgctcagaga caggaagtct tcagggaagg tcacctgagc ccggatgctt ctccatgaga
      421 cacatctcct ccatactcag gactcctctc cgcagttcct gtcccttctc ttaatttaat
      481 cttttttatg tgccgtgtta ttgtattagg tgtcatttcc attatttata ttagtttagc
      541 caaaggataa gtgtccccta tggggatggt ccactgtcac tgtttctctg ctgttgcaaa
      601 tacatggata acacatttga ttctgtgtgt tttcataata aaactttaaa ataaaatgca
//
