LOCUS       NM_001258307            1329 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens coiled-coil domain containing 74B (CCDC74B),
            transcript variant 2, mRNA.
ACCESSION   NM_001258307
VERSION     NM_001258307.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1329)
  AUTHORS   Zhou,H., Zheng,T., Wang,T., Li,Q., Wang,F., Liang,X., Chen,J. and
            Teng,J.
  TITLE     CCDC74A/B are K-fiber crosslinkers required for chromosomal
            alignment
  JOURNAL   BMC Biol 17 (1), 73 (2019)
   PUBMED   31521166
  REMARK    GeneRIF: The authors characterized CCDC74A and CCDC74B as
            microtubule-associated proteins that localize to spindles and are
            important K-fiber crosslinkers required for bipolar spindle
            formation and chromosome alignment.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018804.9.
            
            On Jan 16, 2020 this sequence version replaced NM_001258307.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the coding region, but maintains the reading frame, compared to
            variant 1. The encoded isoform (2) is shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC067771.1, SRR14038192.689328.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409943.8/ ENSP00000386294.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-366               AC018804.9         93316-93681
            367-411             AC018804.9         95115-95159
            412-462             AC018804.9         96245-96295
            463-601             AC018804.9         97132-97270
            602-794             AC018804.9         98057-98249
            795-868             AC018804.9         98332-98405
            869-925             AC018804.9         98481-98537
            926-1329            AC018804.9         98738-99141
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.1"
     gene            1..1329
                     /gene="CCDC74B"
                     /note="coiled-coil domain containing 74B"
                     /db_xref="GeneID:91409"
                     /db_xref="HGNC:HGNC:25267"
     exon            1..366
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1061
                     /gene="CCDC74B"
                     /note="isoform 2 is encoded by transcript variant 2;
                     coiled-coil domain-containing protein 74B"
                     /codon_start=1
                     /product="coiled-coil domain-containing protein 74B
                     isoform 2"
                     /protein_id="NP_001245236.1"
                     /db_xref="CCDS:CCDS58726.1"
                     /db_xref="GeneID:91409"
                     /db_xref="HGNC:HGNC:25267"
                     /translation="MSGAGVAAGTRPPSSPTPGSRRRRQRPSVGVQSLRPQSPQLRQS
                     DPQKRNLDLEKSLQFLQQQHSEMLAKLHEEIEHLKRENKDLRYKLIMNQTSQKKDSLS
                     TSSFQSVKSISNSGKARPQPGSFNKQDSKADVPQKADLEEEPLLHNSKLDKVPGVQGQ
                     ARKEKAEASNAGAACMGNSQHQGRQMGAAAHPPMILPLPLRKPTTLRQCEVLIRELWN
                     TNLLQTQELQHLKSLLEGSQRPQAVPEEASFPRDQEATHFPKVSTKSLSKKCLLLSPP
                     VAERAILPALKQTPKNNFAERQKRLQAMQKRRLHRSVL"
     exon            367..411
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     exon            412..462
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     exon            463..601
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     exon            602..794
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     exon            795..868
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     exon            869..925
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1329
                     /gene="CCDC74B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1308..1313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CCDC74B"
                     /note="hexamer: AATAAA"
     polyA_site      1329
                     /gene="CCDC74B"
                     /note="major polyA site"
ORIGIN      
        1 caaacgcctg gcgccctccg tcgcccggtt tccatggtga cggggcgcca ggctagggcg
       61 gcctggccac tgagccgggg tgcagtggca gcgggagagt acctggcgat ggcgatatga
      121 gcggtgcggg ggtggcggct gggacgcggc cccccagctc gccgaccccg ggctctcggc
      181 gccggcgcca gcgcccctct gtgggcgtcc agtccttgag gccgcagagc ccgcagctca
      241 ggcagagcga cccgcagaaa cggaacctgg acctggagaa gagcctacag ttcctgcagc
      301 agcagcactc ggagatgctg gccaagctcc atgaggagat cgagcatctg aagcgggaaa
      361 acaaggatct ccgttacaag ctcataatga atcagacatc acagaagaaa gacagcctct
      421 ccacgtcaag cttccagtct gtcaagtcca tctctaattc aggcaaggcc aggccccagc
      481 ccggctcctt caacaagcaa gattcaaaag ctgacgtccc ccagaaggcg gacctggaag
      541 aggagcccct acttcacaac agcaagctgg acaaggttcc tggggtacaa gggcaggcca
      601 gaaaggagaa agcagaggcc tctaacgcag gagctgcctg tatggggaac agccagcacc
      661 agggcaggca gatgggggcg gcggcacacc ccccaatgat cctgcccctt cccctgcgaa
      721 agcccaccac acttaggcag tgcgaagtgc tcatccgcga gctgtggaat accaacctcc
      781 tgcagaccca agagctgcag cacctcaagt ccctcctgga agggagccag aggccccagg
      841 cagtcccgga ggaagctagc tttcccaggg accaagaagc cacgcatttc cccaaggtct
      901 ccaccaagag cctctccaag aaatgcctgc ttctgagccc acctgtggcg gagcgtgcca
      961 tcctgcccgc actgaagcag accccgaaga acaactttgc cgagaggcag aagaggctgc
     1021 aggcaatgca gaaacggcgc ctgcatcgct cggtgctttg agctacccgc atctggtcag
     1081 tgccaggccc accaacctgc agctggagat tggctctcta tagcatttcc tgacacttcc
     1141 gctactttta ggcctggcta aattccaaga tagataacac tcaagataga taaagtactt
     1201 gatctccaaa ctgacaaact gtttattttc tagctgttat tttgctattt ggcatttaca
     1261 taaaagcaca cgatgaagca ggtatcgcct tacctgttga aactgaaaat aaagcttgtt
     1321 tatttccaa
//
